Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,901,405	C→T	L272L (CTG→CTA)	bglH ←	carbohydrate‑specific outer membrane porin, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,901,405	0	C	T	100.0%	277.8 / NA	73	L272L (CTG→CTA)	bglH	carbohydrate‑specific outer membrane porin, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/36);  total (37/36)

TTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACCAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGC  >  NC_000913/3901258‑3901552                                                                                                                                                    |                                                                                                                                                    ttCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAg                                                                                                                                                   <  1:203774/150‑1 (MQ=255)      ttGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCCACATCTTCGCGAACTAGCGCGat                                                                                                                                             >  2:975987/1‑151 (MQ=255)          cGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCa                                                                                                                                         <  1:109403/151‑1 (MQ=255)          cGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCa                                                                                                                                         <  1:597773/151‑1 (MQ=255)             gACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAAt                                                                                                                                       <  1:696656/150‑1 (MQ=255)               cATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAAtttt                                                                                                                                    <  1:779160/151‑1 (MQ=255)                aTAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTa                                                                                                                                   <  1:517438/151‑1 (MQ=255)                  aaCGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTAcc                                                                                                                                 >  2:690799/1‑151 (MQ=255)                  aaCGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTAc                                                                                                                                  <  1:1110740/150‑1 (MQ=255)                    cGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCg                                                                                                                               >  1:1129425/1‑151 (MQ=255)                     gACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCgg                                                                                                                              >  1:1059022/1‑151 (MQ=255)                             aCCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACt                                                                                                                       <  1:489166/150‑1 (MQ=255)                              ccATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACt                                                                                                                       <  2:43419/149‑1 (MQ=255)                               cATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTc                                                                                                                    <  1:306934/151‑1 (MQ=255)                                   aaGGTGGCTTTATCCCATAACGGGATCTCTTTATAGCGTAAATCTATGGAATTGGCATTAATCTGCTGTTTGTATTGCACGCTGCGATCGTAATCATCGACATCTTCTCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCa                                                                                                                  >  1:907677/1‑149 (MQ=255)                                   aaGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGt                                                                                                                >  1:732729/1‑151 (MQ=255)                                      gTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGtttt                                                                                                             >  2:1107003/1‑151 (MQ=255)                                        ggCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTcc                                                                                                           <  2:986038/151‑1 (MQ=255)                                        ggCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTcc                                                                                                           <  1:357076/151‑1 (MQ=255)                                           tttATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTcc                                                                                                           >  2:337884/1‑148 (MQ=255)                                             tATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGAcc                                                                                                      >  2:127204/1‑151 (MQ=255)                                              aTCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCt                                                                                                     >  2:159266/1‑151 (MQ=255)                                                cccATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTac                                                                                                   <  2:279403/151‑1 (MQ=255)                                                  cATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTacac                                                                                                 >  1:509851/1‑151 (MQ=255)                                                   aTAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACAcc                                                                                                >  2:510653/1‑151 (MQ=255)                                                         ggATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGc                                                                                          >  2:743783/1‑151 (MQ=255)                                                          gATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCg                                                                                         >  2:325204/1‑151 (MQ=255)                                                            tatCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGc                                                                                       >  2:859137/1‑151 (MQ=255)                                                             atCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGc                                                                                       <  2:1201383/150‑1 (MQ=255)                                                                 ttATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCa                                                                                      <  2:803924/147‑1 (MQ=255)                                                                 ttATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATc                                                                                    <  2:454011/149‑1 (MQ=255)                                                                 ttATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCa                                                                                   <  1:404026/150‑1 (MQ=255)                                                                       cGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCt                                                                            <  2:684615/151‑1 (MQ=255)                                                                       cGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCt                                                                            >  1:454082/1‑151 (MQ=255)                                                                         tAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTGCGCTg                                                                           <  2:764223/150‑1 (MQ=255)                                                                         tAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGc                                                                          >  2:215444/1‑151 (MQ=255)                                                                            aTCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGtt                                                                       >  1:282225/1‑151 (MQ=255)                                                                            aTCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGtt                                                                       >  2:864610/1‑151 (MQ=255)                                                                              cAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGtttt                                                                     <  2:794681/151‑1 (MQ=255)                                                                              cAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGttt                                                                      <  2:546317/150‑1 (MQ=255)                                                                                     attggtattAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTc                                                               >  2:892239/1‑150 (MQ=255)                                                                                     attggtattAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTc                                                               <  2:440510/150‑1 (MQ=255)                                                                                      ttggtattAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTc                                                               >  1:1146037/1‑149 (MQ=255)                                                                                       tggtattAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCa                                                              >  1:841243/1‑149 (MQ=255)                                                                                        ggtattAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGc                                                           >  1:625945/1‑151 (MQ=255)                                                                                        ggtattAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGc                                                           <  2:1027342/151‑1 (MQ=255)                                                                                           attAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCAt                                                         <  1:1040096/150‑1 (MQ=255)                                                                                           attAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATc                                                        >  1:123221/1‑151 (MQ=255)                                                                                               aTCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTgg                                                     >  2:363758/1‑150 (MQ=255)                                                                                                   gctgTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTc                                                >  1:763867/1‑151 (MQ=255)                                                                                                       tttgtttTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAAtt                                            >  1:991396/1‑151 (MQ=255)                                                                                                           ttttGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCg                                        >  2:299737/1‑151 (MQ=255)                                                                                                            tttGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCg                                        <  1:306349/150‑1 (MQ=255)                                                                                                              tGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGgc                                      <  1:221569/150‑1 (MQ=255)                                                                                                               gCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGgcgc                                    <  1:1161853/151‑1 (MQ=255)                                                                                                                cAGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGgcgc                                    >  1:487113/1‑150 (MQ=255)                                                                                                                 aGGCTGCGATCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCAc                                  >  1:836672/1‑151 (MQ=255)                                                                                                                         aTCGTAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTa                          <  2:945563/151‑1 (MQ=255)                                                                                                                            gTAATCATCGACATCTTCGCGAACTAGCGCGATATCACTTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTaccc                       >  2:215594/1‑151 (MQ=255)                                                                                                                             tAATCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTaccc                       >  2:477045/1‑150 (MQ=255)                                                                                                                              aaTCATCGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTaccca                      <  2:785260/150‑1 (MQ=255)                                                                                                                               atcatcGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTaccca                      <  2:192300/149‑1 (MQ=255)                                                                                                                                 catcGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTacccaccc                   >  2:773937/1‑150 (MQ=255)                                                                                                                                   tcGACATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAg                 <  1:80112/150‑1 (MQ=255)                                                                                                                                      aCATCTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAAt             >  2:670267/1‑151 (MQ=255)                                                                                                                                          cTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGc         >  2:294262/1‑151 (MQ=255)                                                                                                                                          cTTCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGCCGCACCGTGTTTACCCACCCAGAAATCAGc         >  1:179302/1‑151 (MQ=255)                                                                                                                                            tCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCa           <  2:1184707/147‑1 (MQ=255)                                                                                                                                            tCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAg          <  2:710124/148‑1 (MQ=255)                                                                                                                                            tCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAg          <  2:1015747/148‑1 (MQ=255)                                                                                                                                            tCGCGAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAg          <  2:189442/148‑1 (MQ=255)                                                                                                                                                gAACTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTgg   >  1:20824/1‑151 (MQ=255)                                                                                                                                                 aaCTAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGgc  <  1:869089/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TTCGTTTGCCGTGACATAACGACCACTTACCATTAAGGTGGCTTTATCCCATAACGGGATATCTTTATAGCGTAAATCAATGGTATTGGTATTAATCTGCTGTTTGTTTTGCAGGCTGCGATCGTAATCATCGACATCTTCGCGAACCAGCGCGATATCAATTTTACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGC  >  NC_000913/3901258‑3901552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑