Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,951,681 T→C pseudogene (140/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,6810TC95.3% 297.9 / 3.5 86pseudogene (140/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base C (48/34);  total (48/38)
Fisher's exact test for biased strand distribution p-value = 3.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

AAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCT  >  NC_000913/3951534‑3951827
                                                                                                                                                   |                                                                                                                                                  
aaaTCAATGACTGGCAGCAACACGGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCggg                                                                                                                                                 <  2:974459/151‑1 (MQ=255)
    cAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGgca                                                                                                                                              <  2:334054/150‑1 (MQ=255)
    cAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGgca                                                                                                                                              <  1:843014/150‑1 (MQ=255)
    cAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGgc                                                                                                                                               >  2:195328/1‑149 (MQ=255)
          cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCAcc                                                                                                                                         >  2:434066/1‑149 (MQ=255)
            ggCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCTTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGAt                                                                                                                                     <  1:512865/151‑1 (MQ=255)
                 caACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTggg                                                                                                                                >  1:198266/1‑151 (MQ=255)
                     acTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTgc                                                                                                                            >  1:1017771/1‑151 (MQ=255)
                        gcgcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCgca                                                                                                                         <  2:563021/151‑1 (MQ=255)
                        gcgcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCgca                                                                                                                         <  1:448533/151‑1 (MQ=255)
                           cgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCgcagc                                                                                                                       >  2:702056/1‑150 (MQ=255)
                             cAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGcaca                                                                                                                    <  1:292746/151‑1 (MQ=255)
                             cAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGcac                                                                                                                     >  2:1141457/1‑150 (MQ=255)
                             cAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGcac                                                                                                                     >  2:224667/1‑150 (MQ=255)
                                  gCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCg                                                                                                                 <  2:1053561/149‑1 (MQ=255)
                                     tgatgaACATTCCTGTCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCcac                                                                                                            >  1:664109/1‑151 (MQ=255)
                                      gatgaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCcaca                                                                                                           <  2:1144212/151‑1 (MQ=255)
                                         gaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACt                                                                                                         <  2:880600/150‑1 (MQ=255)
                                         gaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTc                                                                                                        <  2:270188/151‑1 (MQ=255)
                                                 cTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCgg                                                                                                  >  2:313866/1‑149 (MQ=255)
                                                   ggCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGaa                                                                                                <  2:375769/149‑1 (MQ=255)
                                                      gTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAAttt                                                                                           >  2:955508/1‑151 (MQ=255)
                                                        tACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTtc                                                                                          <  2:583229/150‑1 (MQ=255)
                                                          cGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTtcatc                                                                                       >  2:61095/1‑151 (MQ=255)
                                                                 cccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTcc                                                                                 <  1:527156/150‑1 (MQ=255)
                                                                 cccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTcc                                                                                 <  2:758146/150‑1 (MQ=255)
                                                                 cccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCa                                                                                <  1:420594/151‑1 (MQ=255)
                                                                  ccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTcc                                                                                 >  1:471946/1‑149 (MQ=255)
                                                                   cGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGc                                                                              <  1:1213635/151‑1 (MQ=255)
                                                                        aCGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGt                                                                           <  1:817301/149‑1 (MQ=255)
                                                                          gCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGttt                                                                         >  2:258378/1‑149 (MQ=255)
                                                                          gCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGttt                                                                         >  2:594554/1‑149 (MQ=255)
                                                                          gCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGttt                                                                         >  1:138109/1‑149 (MQ=255)
                                                                          gCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGttt                                                                         >  1:367892/1‑149 (MQ=255)
                                                                          gCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAg                                                                       <  2:1188157/151‑1 (MQ=255)
                                                                           cTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAgg                                                                      >  2:520539/1‑151 (MQ=255)
                                                                             aTCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAgg                                                                      <  1:482020/149‑1 (MQ=255)
                                                                                 aCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCAt                                                                >  2:1028363/1‑151 (MQ=255)
                                                                                 aCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCAt                                                                >  1:209394/1‑151 (MQ=255)
                                                                                      ccGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGtt                                                           <  2:564706/151‑1 (MQ=255)
                                                                                       cGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGttt                                                          >  2:1016153/1‑151 (MQ=255)
                                                                                       cGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGttt                                                          >  1:235906/1‑151 (MQ=255)
                                                                                        gttgttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGtttt                                                         <  2:873618/151‑1 (MQ=255)
                                                                                        gttgttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGtttt                                                         >  2:418357/1‑151 (MQ=255)
                                                                                          tgttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTgg                                                       >  2:445049/1‑151 (MQ=255)
                                                                                          tgttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTgg                                                       >  2:127359/1‑151 (MQ=255)
                                                                                          tgttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTg                                                        <  2:307560/150‑1 (MQ=255)
                                                                                           gttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGTGGCAGCACCAGACGTGGGCTGCGCAGCACATCGCCCACTCTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTgg                                                       <  1:910731/150‑1 (MQ=255)
                                                                                            ttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGtt                                                     >  2:1019637/1‑151 (MQ=255)
                                                                                              gttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGttt                                                    >  2:1009827/1‑150 (MQ=255)
                                                                                              gttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGttt                                                    <  2:344616/150‑1 (MQ=255)
                                                                                              gttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTa                                                   <  1:806050/151‑1 (MQ=255)
                                                                                                 aaaaCAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcg                                                >  2:1104517/1‑151 (MQ=255)
                                                                                                 aaaaCAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcg                                                >  1:913713/1‑151 (MQ=255)
                                                                                                  aaaCAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcgg                                               >  2:866736/1‑151 (MQ=255)
                                                                                                  aaaCAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcgg                                               >  1:872146/1‑151 (MQ=255)
                                                                                                   aacaacTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcggc                                              >  1:415946/1‑151 (MQ=255)
                                                                                                     caacTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcggcgg                                            >  1:542083/1‑151 (MQ=255)
                                                                                                     caacTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcggcgg                                            <  1:1152418/151‑1 (MQ=255)
                                                                                                       acTGTCGGATCGTAAACCTGCGGATTGCGACGTGACCACAGATGCGGGGCACCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcggcggc                                           >  2:793634/1‑150 (MQ=255)
                                                                                                          gTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGt                                        <  2:469261/150‑1 (MQ=255)
                                                                                                              gATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGgcg                                   <  2:1188148/151‑1 (MQ=255)
                                                                                                                  gTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCCCCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGcaca                               <  2:719340/151‑1 (MQ=255)
                                                                                                                  gTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGGTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGCTTAGGTACCATGGGGTTTGGTTTACcggcggagg                                         >  1:32405/1‑141 (MQ=255)
                                                                                                                  gTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGcaca                               <  1:245292/151‑1 (MQ=255)
                                                                                                                    aaaCCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGcaca                               >  2:210289/1‑149 (MQ=255)
                                                                                                                    aaaCCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGcaca                               >  1:621034/1‑149 (MQ=255)
                                                                                                                            ggATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTcgcgcg                      >  1:144493/1‑150 (MQ=255)
                                                                                                                            ggATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTcgcgcg                      >  2:141379/1‑150 (MQ=255)
                                                                                                                             gATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGGcgcgcg                      >  2:1000080/1‑149 (MQ=255)
                                                                                                                                 gCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCTCGCGACCGa                 >  1:86555/1‑150 (MQ=255)
                                                                                                                                 gCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGa                 <  1:873271/150‑1 (MQ=255)
                                                                                                                                  cgtcgtGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGaa                >  1:457175/1‑150 (MQ=255)
                                                                                                                                  cgtcgtGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAAc               <  1:307328/151‑1 (MQ=255)
                                                                                                                                   gtcgtGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCGCATCGCCCACTCTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACg              <  1:1175718/151‑1 (MQ=255)
                                                                                                                                   gtcgtGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCCCGACCGaa                >  1:492268/1‑149 (MQ=255)
                                                                                                                                     cgtGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGAt            >  2:989915/1‑151 (MQ=255)
                                                                                                                                     cgtGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGAt            <  2:630778/151‑1 (MQ=255)
                                                                                                                                     cgtGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGAt            <  1:434052/151‑1 (MQ=255)
                                                                                                                                      gtGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGa             >  1:192545/1‑149 (MQ=255)
                                                                                                                                      gtGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGAt            >  2:1138214/1‑150 (MQ=255)
                                                                                                                                        gACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGATGGCGCACAAGGCGCGCGACCGCACCAAAcc         >  1:87400/1‑151 (MQ=255)
                                                                                                                                           cacaGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGtt      <  2:48718/151‑1 (MQ=255)
                                                                                                                                           cacaGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGATGGCGCACAAGTCGCGCGACCGAACGATACCGtt      >  1:662024/1‑151 (MQ=255)
                                                                                                                                              aGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGCAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGGTTTGGCTTACCGGCGCCGGTTTGCGCCCAAGGTGGCCGACCGGACGATACCGtt      >  1:1121478/1‑148 (MQ=255)
                                                                                                                                               gATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCt  <  1:96279/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGTCT  >  NC_000913/3951534‑3951827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: