Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,970,392 C→T P87L (CCA→CTA)  wecB → UDP‑N‑acetyl glucosamine‑2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,970,3920CT98.8% 319.1 / NA 86P87L (CCA→CTA) wecBUDP‑N‑acetyl glucosamine‑2‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (40/45);  minor base G (0/1);  total (40/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTAC  >  NC_000913/3970244‑3970539
                                                                                                                                                    |                                                                                                                                                   
cagcaTCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAg                                                                                                                                                   <  1:310493/151‑1 (MQ=255)
      cGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGAcgtcg                                                                                                                                             >  2:172591/1‑151 (MQ=255)
      cGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGAcgtcg                                                                                                                                             >  2:392515/1‑151 (MQ=255)
      cGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGAcggc                                                                                                                                              <  1:302070/150‑3 (MQ=255)
        ggAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTg                                                                                                                                           <  2:521375/151‑1 (MQ=255)
         gagaTGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGc                                                                                                                                          >  1:692517/1‑151 (MQ=255)
              gCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGtt                                                                                                                                     <  2:220806/151‑1 (MQ=255)
              gCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGt                                                                                                                                      <  2:1126874/150‑1 (MQ=255)
                 cGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGATGTCGTGCTGGTTCAc                                                                                                                                  >  1:1186457/1‑151 (MQ=255)
                  ggtCAGGTTCTGAAACTCTTTTCCTTTGTCCCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTGGCCTTCGTGCTGGTTctc                                                                                                                                  <  2:615078/148‑3 (MQ=255)
                  gATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACg                                                                                                                                 >  1:320437/1‑151 (MQ=255)
                   aTCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACgg                                                                                                                                >  2:962439/1‑151 (MQ=255)
                      aGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCg                                                                                                                              >  1:412546/1‑150 (MQ=255)
                         tGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATac                                                                                                                          >  1:311706/1‑151 (MQ=255)
                          gCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATacg                                                                                                                         >  1:600931/1‑151 (MQ=255)
                          gCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATac                                                                                                                          <  2:1166082/150‑1 (MQ=255)
                          gCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATa                                                                                                                           <  1:857492/149‑1 (MQ=255)
                           cTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATacg                                                                                                                         <  1:818199/150‑1 (MQ=255)
                             gAAACTCTTTTCCATTGTGCCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACGAGACGTCGTGCTGGTTCACGGCGATacgacg                                                                                                                      <  1:275593/151‑1 (MQ=255)
                             gAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATacgac                                                                                                                       <  2:277288/150‑1 (MQ=255)
                             gAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATacgac                                                                                                                       <  1:320542/150‑1 (MQ=255)
                             gAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATacga                                                                                                                        <  1:67936/149‑1 (MQ=255)
                                aCTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATacgacgacg                                                                                                                   <  2:37157/151‑1 (MQ=255)
                                  tctTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATacgacgacgac                                                                                                                 >  1:974422/1‑151 (MQ=255)
                                     tttCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATacgacgacgac                                                                                                                 <  1:782084/148‑1 (MQ=255)
                                     tttCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCt                                                                                                              >  1:743319/1‑151 (MQ=255)
                                        ccATTGTGCCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATATCCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTgg                                                                                                            <  2:47479/150‑1 (MQ=255)
                                           ttGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGCCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAAc                                                                                                        >  2:44934/1‑151 (MQ=255)
                                            tGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAAcc                                                                                                       >  2:912269/1‑151 (MQ=255)
                                              tACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAAcc                                                                                                       >  1:851403/1‑149 (MQ=255)
                                              tACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAAcc                                                                                                       >  1:769245/1‑149 (MQ=255)
                                                   gACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCt                                                                                                  <  1:1048005/149‑1 (MQ=255)
                                                      tACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTggcgg                                                                                             >  1:691898/1‑151 (MQ=255)
                                                       aCGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTggcggc                                                                                            <  2:765877/151‑1 (MQ=255)
                                                       aCGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTggcg                                                                                              <  2:633384/149‑1 (MQ=255)
                                                       aCGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGGCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTggcggc                                                                                            <  1:109685/151‑1 (MQ=255)
                                                           tctcAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGttt                                                                                        >  2:937004/1‑151 (MQ=255)
                                                             tcAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGtttt                                                                                       >  2:675775/1‑150 (MQ=255)
                                                              cAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGt                                                                                          <  2:1082849/146‑1 (MQ=255)
                                                              cAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTAt                                                                                     <  1:1001355/151‑1 (MQ=255)
                                                               aaCATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATc                                                                                    >  1:622009/1‑151 (MQ=255)
                                                                aCATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCa                                                                                   <  2:45033/151‑1 (MQ=255)
                                                                 cATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATc                                                                                    >  1:179884/1‑149 (MQ=255)
                                                                  aTAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAg                                                                                  <  1:431477/150‑1 (MQ=255)
                                                                    aaTGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGt                                                                               <  1:935843/151‑1 (MQ=255)
                                                                        cAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTc                                                                           <  2:258917/151‑1 (MQ=255)
                                                                           ccAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTg                                                                        >  1:384540/1‑151 (MQ=255)
                                                                            cAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGt                                                                       <  2:334046/151‑1 (MQ=255)
                                                                            cAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGt                                                                       <  1:939943/151‑1 (MQ=255)
                                                                            cAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGt                                                                       >  1:586057/1‑151 (MQ=255)
                                                                               gACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTgg                                                                    <  2:643003/151‑1 (MQ=255)
                                                                                aCAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTgg                                                                    >  2:1145347/1‑150 (MQ=255)
                                                                                 cAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTc                                                                  >  1:1084884/1‑151 (MQ=255)
                                                                                  aGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTc                                                                  >  1:710621/1‑150 (MQ=255)
                                                                                      ccTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGtt                                                             <  1:798436/151‑1 (MQ=255)
                                                                                         gACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTg                                                            <  2:509034/149‑1 (MQ=255)
                                                                                          aCAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAg                                                          <  1:803978/150‑1 (MQ=255)
                                                                                           cAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGc                                                        <  1:369135/151‑1 (MQ=255)
                                                                                                 tAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCt                                                  >  2:512888/1‑151 (MQ=255)
                                                                                                  aaCCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTg                                                 >  1:1105534/1‑151 (MQ=255)
                                                                                                  aaCCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTg                                                 <  1:170668/151‑1 (MQ=255)
                                                                                                  aaCCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTg                                                 <  2:626719/151‑1 (MQ=255)
                                                                                                  aaCCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTg                                                 >  2:299352/1‑151 (MQ=255)
                                                                                                   aCCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTgc                                                <  2:1081284/151‑1 (MQ=255)
                                                                                                         cGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCAc                                            <  2:771774/149‑1 (MQ=255)
                                                                                                          ggATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACggg                                         >  2:1078565/1‑151 (MQ=255)
                                                                                                                 ggAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGAtctc                                  <  1:503980/151‑1 (MQ=255)
                                                                                                                  gAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATctct                                 <  2:383584/151‑1 (MQ=255)
                                                                                                                   aaGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTa                                <  2:174174/151‑1 (MQ=255)
                                                                                                                   aaGGGCTAAAACCTATTCTTGCCGAGTTCAAACTAAACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTa                                <  1:416253/151‑1 (MQ=255)
                                                                                                                     gggCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTAtt                              >  2:360345/1‑151 (MQ=255)
                                                                                                                       gCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCg                            <  1:1210038/151‑1 (MQ=255)
                                                                                                                       gCTAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCg                            <  1:972196/151‑1 (MQ=255)
                                                                                                                         tAAAACCTATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGcc                          >  1:506056/1‑151 (MQ=255)
                                                                                                                                tATTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGGGGcc                    >  1:377048/1‑150 (MQ=255)
                                                                                                                                 aTTCTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGcc                    <  2:1096487/149‑1 (MQ=255)
                                                                                                                                    cTTGCCGAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGa                 >  1:715893/1‑149 (MQ=255)
                                                                                                                                          gAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCt          >  1:1147623/1‑150 (MQ=255)
                                                                                                                                          gAGTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTAGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCt          >  2:978459/1‑150 (MQ=255)
                                                                                                                                            gTTCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAAc       >  2:559431/1‑151 (MQ=255)
                                                                                                                                             ttCAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAAcc      >  2:748420/1‑151 (MQ=255)
                                                                                                                                               cAAACTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGt    <  1:153713/151‑1 (MQ=255)
                                                                                                                                                aaaCTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGt    >  2:358737/1‑150 (MQ=255)
                                                                                                                                                 aaCTAGACGTCGTGCTGGTTCACGGCGATACGATGACGACGATGGCATCCAGCCTGGCGGAGTTTTATCAGCGTATTCCCGTTGGTCCCGTTGAGGCTGGTCTGGGCACTGGAGATCTCTATTCGCCGTGGCCGGAAGAGGCTAAcc      >  1:135647/1‑147 (MQ=255)
                                                                                                                                                 aaCTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTAc  >  1:434110/1‑151 (MQ=255)
                                                                                                                                                 aaCTAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTAc  <  1:434371/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTAC  >  NC_000913/3970244‑3970539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: