Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,086,147 G→A A44A (GCG→GCA fdhD → formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,086,1470GA98.6% 248.1 / NA 71A44A (GCG→GCAfdhDformate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (38/32);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGG  >  NC_000913/4086002‑4086293
                                                                                                                                                 |                                                                                                                                                  
cTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGt                                                                                                                                               >  2:1044596/1‑151 (MQ=255)
cTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTAAAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTgg                                                                                                                                                >  1:806144/1‑150 (MQ=255)
  ggTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTg                                                                                                                                                 <  1:294857/147‑1 (MQ=255)
  ggTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCt                                                                                                                                             >  2:944633/1‑151 (MQ=255)
           aaaGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCa                                                                                                                                    <  1:703898/151‑1 (MQ=255)
             aGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCAtt                                                                                                                                  <  2:1059144/151‑1 (MQ=255)
                 aaaaaaaCACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGc                                                                                                                              <  2:21416/151‑1 (MQ=255)
                    aaaaCACAGCGTAAAGAAATCGAATATGTGACATATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACTGTTAGGTGAGGTGGCGGACAAAGATCCTGTTGCACTTGTCTGCAACGGCGTTTCGGAt                                                                                                                            >  1:570176/1‑150 (MQ=255)
                     aaaCACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCAtgt                                                                                                                          <  2:611293/151‑1 (MQ=255)
                      aaCACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCAtgtg                                                                                                                         >  2:18449/1‑151 (MQ=255)
                          caGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATgtggt                                                                                                                       >  1:194879/1‑149 (MQ=255)
                           aGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGtgat                                                                                                                    <  1:642094/151‑1 (MQ=255)
                              gAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTgatgat                                                                                                                 <  1:1012337/151‑1 (MQ=255)
                                aaaGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATgg                                                                                                               <  1:844431/151‑1 (MQ=255)
                                aaaGAAATCGAAAATGTGACAAATATCACAGGCGTTCGTCGAATTGAGTTATGGCGGCGAGATGATTTACAACACCCACGGTTAGTTGAGGTCGTGGAAGAAGTTCCCGGTGCACTGGCCTACAACGGCATTTCGAATGTGGtga                                                                                                                     >  1:1020569/1‑145 (MQ=255)
                                  aGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCg                                                                                                             <  2:1173799/151‑1 (MQ=255)
                                    aaaTCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTc                                                                                                           >  2:413272/1‑151 (MQ=255)
                                     aaTCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCg                                                                                                          <  2:800606/151‑1 (MQ=255)
                                        cGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGccc                                                                                                       >  1:1043038/1‑151 (MQ=255)
                                        cGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGcc                                                                                                        <  1:372526/150‑1 (MQ=255)
                                         gAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGccc                                                                                                       >  1:929185/1‑150 (MQ=255)
                                          aaaaTGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCaa                                                                                                     <  1:568565/151‑1 (MQ=255)
                                             aTGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGa                                                                                                  <  2:705782/151‑1 (MQ=255)
                                               gtgACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGAc                                                                                                 <  2:404539/150‑1 (MQ=255)
                                               gtgACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGATAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGGGATGATGGAGTCGCCCAAAGAcc                                                                                                >  2:979270/1‑151 (MQ=255)
                                                 gACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGAGGAAGAAGCTCCCGTTGCACTGGACTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCt                                                                                               >  2:132313/1‑150 (MQ=255)
                                                 gACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGGGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCtt                                                                                              >  1:726001/1‑151 (MQ=255)
                                                  aCAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCtt                                                                                              >  2:666474/1‑150 (MQ=255)
                                                   cAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTg                                                                                             >  2:92705/1‑150 (MQ=255)
                                                    aaaTATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGa                                                                                            <  1:1064404/150‑1 (MQ=255)
                                                      atatCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTGAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGt                                                                                          <  1:349405/150‑1 (MQ=255)
                                                        atCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTAc                                                                                        <  2:605453/150‑1 (MQ=255)
                                                         tCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACtt                                                                                      >  2:481445/1‑151 (MQ=255)
                                                         tCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGGACtt                                                                                      >  1:555326/1‑151 (MQ=255)
                                                            caGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTgc                                                                                   <  1:770970/151‑1 (MQ=255)
                                                              ggTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTgcgc                                                                                 <  2:429145/151‑1 (MQ=255)
                                                                tgtTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTc                                                                               >  2:339347/1‑151 (MQ=255)
                                                                       cAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGGCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGCGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGttttt                                                                        >  1:418238/1‑151 (MQ=255)
                                                                         aaTTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCg                                                                      <  2:45599/151‑1 (MQ=255)
                                                                         aaTTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCg                                                                      >  2:850151/1‑151 (MQ=255)
                                                                            tGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCtt                                                                   >  1:1055353/1‑151 (MQ=255)
                                                                            tGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCtt                                                                   >  2:796627/1‑151 (MQ=255)
                                                                               gTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTcc                                                                <  2:526468/151‑1 (MQ=255)
                                                                                 tATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGGTTTTCGCTTTCCg                                                               >  1:160460/1‑150 (MQ=255)
                                                                                   tGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAgg                                                            >  1:295365/1‑151 (MQ=255)
                                                                                      cggcgTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGa                                                         <  2:1175245/151‑1 (MQ=255)
                                                                                       ggcgTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGAt                                                        <  2:379056/151‑1 (MQ=255)
                                                                                         cgTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGAtt                                                       >  1:1122918/1‑150 (MQ=255)
                                                                                            gatgatTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCg                                                   <  1:744249/151‑1 (MQ=255)
                                                                                              tgatTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGa                                                  >  2:496702/1‑150 (MQ=255)
                                                                                               gatTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGaaa                                                <  2:1114346/151‑1 (MQ=255)
                                                                                                 tttACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGt                                              >  2:995157/1‑151 (MQ=255)
                                                                                                  ttACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGt                                              <  1:1211636/150‑1 (MQ=255)
                                                                                                    acaacaCCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCcg                                           <  2:194885/151‑1 (MQ=255)
                                                                                                          cccACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGat                                     >  2:24068/1‑151 (MQ=255)
                                                                                                          cccACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGat                                     <  1:995916/151‑1 (MQ=255)
                                                                                                           ccACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGata                                    <  2:1061694/151‑1 (MQ=255)
                                                                                                              cGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCt                                 <  2:790979/151‑1 (MQ=255)
                                                                                                                gTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTc                               >  2:412654/1‑151 (MQ=255)
                                                                                                                  tAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCgg                             >  2:337144/1‑151 (MQ=255)
                                                                                                                      tGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATg                         >  1:679966/1‑151 (MQ=255)
                                                                                                                         ggTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGAt                      <  2:514493/151‑1 (MQ=255)
                                                                                                                          gTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGAt                      >  1:194166/1‑150 (MQ=255)
                                                                                                                            cgcgGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGt                    <  1:316672/150‑1 (MQ=255)
                                                                                                                             gcgGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCg                  >  1:347477/1‑151 (MQ=255)
                                                                                                                              cgGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGt                 <  2:697965/151‑1 (MQ=255)
                                                                                                                               ggAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGtt                >  1:399837/1‑151 (MQ=255)
                                                                                                                                    aagTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGttccttc           >  1:382445/1‑151 (MQ=255)
                                                                                                                                           cGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAAt    >  2:377172/1‑151 (MQ=255)
                                                                                                                                           cGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAAt    >  1:894220/1‑151 (MQ=255)
                                                                                                                                              tGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATgg  >  1:310899/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATGG  >  NC_000913/4086002‑4086293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: