Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,181,706 C→T G154G (GGC→GGT)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,181,7060CT98.5% 227.8 / ‑4.1 68G154G (GGC→GGT) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (36/30);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

GAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGT  >  NC_000913/4181560‑4181850
                                                                                                                                                  |                                                                                                                                                
gAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGtct                                                                                                                                              >  2:689770/1‑151 (MQ=255)
 aGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGtctt                                                                                                                                             <  2:360723/151‑1 (MQ=255)
    gcgAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTcttctt                                                                                                                                          <  1:77947/151‑1 (MQ=255)
     cgAAGCGGCGGAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTAGCAACGGTACTGAGCGTGTTATCGTTTGCCAGCTGCACCGTAGTCCGGTTGtcttct                                                                                                                                           <  1:265343/149‑1 (MQ=255)
         gcgcCGGAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACt                                                                                                                                     <  2:120949/151‑1 (MQ=255)
             cGGAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCg                                                                                                                                  >  2:401941/1‑150 (MQ=255)
             cGGAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCg                                                                                                                                  >  2:23421/1‑150 (MQ=255)
               gAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGAc                                                                                                                                >  2:154027/1‑150 (MQ=255)
                  ggCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAg                                                                                                                            <  2:161532/151‑1 (MQ=255)
                  ggCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAg                                                                                                                            <  2:285634/151‑1 (MQ=255)
                    cACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGt                                                                                                                          >  2:558734/1‑151 (MQ=255)
                    cACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGAACCTTTGTTATCAACGGCACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGt                                                                                                                          >  2:936793/1‑151 (MQ=255)
                          aaaaGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAAc                                                                                                                     <  2:641866/150‑1 (MQ=255)
                           aaaGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAAccc                                                                                                                   >  2:1134953/1‑151 (MQ=255)
                            aaGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGGCCTGAGCGTTTTATCGTTTCCCAGCTGCACCGTAGTCGGGGAGTCTTCTTTGAGTCCGACAAAGGTAAAAcc                                                                                                                    >  2:733404/1‑149 (MQ=255)
                                 aTTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCtt                                                                                                             <  2:1066358/151‑1 (MQ=255)
                                  ttAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTc                                                                                                            >  2:452928/1‑151 (MQ=255)
                                  ttAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTc                                                                                                            >  2:624947/1‑151 (MQ=255)
                                  ttAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTc                                                                                                            >  2:1019860/1‑151 (MQ=255)
                                    aaaGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCgg                                                                                                          >  2:385051/1‑151 (MQ=255)
                                     aaGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCggg                                                                                                         <  2:216588/151‑1 (MQ=255)
                                      aGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGt                                                                                                        <  2:104993/151‑1 (MQ=255)
                                               agTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTg                                                                                               <  2:187594/151‑1 (MQ=255)
                                               agTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTg                                                                                               >  1:77607/1‑151 (MQ=255)
                                               agTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGCAAAACCCACTCTTCGGGTAAAGTg                                                                                                  >  2:980293/1‑148 (MQ=255)
                                                gTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGt                                                                                              >  2:931737/1‑151 (MQ=255)
                                                  cTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGtat                                                                                            >  2:635892/1‑151 (MQ=255)
                                                   tACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGtat                                                                                            >  2:607919/1‑150 (MQ=255)
                                                    aCATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGtata                                                                                           >  2:888587/1‑150 (MQ=255)
                                                    aCATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATaa                                                                                          <  1:1019799/151‑1 (MQ=255)
                                                    aCATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATaa                                                                                          <  1:46101/151‑1 (MQ=255)
                                                     cATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAAc                                                                                         <  2:234636/151‑1 (MQ=255)
                                                      aTGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAAcg                                                                                        <  2:625887/151‑1 (MQ=255)
                                                       tGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAAcgc                                                                                       >  2:749539/1‑151 (MQ=255)
                                                         ggCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAAcgc                                                                                       <  1:257166/149‑1 (MQ=255)
                                                         ggCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAAcgcgc                                                                                     >  2:728389/1‑151 (MQ=255)
                                                           cGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGt                                                                                   <  2:496155/151‑1 (MQ=255)
                                                            gAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGt                                                                                   >  2:428636/1‑150 (MQ=255)
                                                               aTTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTatc                                                                                >  2:939365/1‑150 (MQ=255)
                                                               aTTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTatc                                                                                >  1:1183573/1‑150 (MQ=255)
                                                                   cGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATccc                                                                           >  1:35653/1‑151 (MQ=255)
                                                                    gCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCt                                                                          >  1:1069002/1‑151 (MQ=255)
                                                                    gCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCAAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGCAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATctct                                                                          >  1:3483/1‑151 (MQ=255)
                                                                     cTCATGACAGACAACGGTCCCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGTCATAGGTAAACCCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTatcg                                                                               <  1:726327/145‑2 (MQ=255)
                                                                        aTGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTAcc                                                                      >  1:337015/1‑151 (MQ=255)
                                                                         tGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCg                                                                     >  1:233724/1‑151 (MQ=255)
                                                                              gacaACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGtt                                                                >  2:864412/1‑151 (MQ=255)
                                                                                caACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTcc                                                              >  1:168528/1‑151 (MQ=255)
                                                                                    ggTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCctggc                                                          >  1:574352/1‑151 (MQ=255)
                                                                                     gTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCctggct                                                         <  2:267180/151‑1 (MQ=255)
                                                                                            tGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTc                                                  >  1:590417/1‑151 (MQ=255)
                                                                                                aTCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAAt                                              >  2:1179551/1‑151 (MQ=255)
                                                                                                                    tATCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTg                          >  1:963417/1‑151 (MQ=255)
                                                                                                                     aTCGTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACATg                          <  1:441762/150‑1 (MQ=255)
                                                                                                                        gTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTCCCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTcg                      <  1:674545/151‑1 (MQ=255)
                                                                                                                        gTTTCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTcg                      <  1:1142298/151‑1 (MQ=255)
                                                                                                                         tttCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTcgt                     >  2:5700/1‑151 (MQ=255)
                                                                                                                          ttCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTcgt                     >  1:162907/1‑150 (MQ=255)
                                                                                                                          ttCCCAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTcgt                     >  1:281179/1‑150 (MQ=255)
                                                                                                                            cccAGCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTcgtac                   <  1:1156902/150‑1 (MQ=255)
                                                                                                                                gCTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTAt               <  1:155850/150‑1 (MQ=255)
                                                                                                                                 cTGCACCGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCg             >  1:251629/1‑151 (MQ=255)
                                                                                                                                      ccGTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGt        <  1:180570/151‑1 (MQ=255)
                                                                                                                                        gTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCg      <  2:26724/151‑1 (MQ=255)
                                                                                                                                        gTAGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCg      <  1:936737/151‑1 (MQ=255)
                                                                                                                                          aGTCCGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGcc    <  1:1055463/151‑1 (MQ=255)
                                                                                                                                             ccGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCCCTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTCCCTTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGt  <  1:733379/150‑1 (MQ=255)
                                                                                                                                             ccGGGTGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGt  <  2:395419/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GAGCGCGAAGCGCCGGAAGGCACCGTAAAAGACATTAAAGAACAAGAAGTCTACATGGGCGAAATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGCGCGTATCATCCCTTACCGTGGTTCCTGGCTGGACTTCGAATTCGATCCGAAGGACAACCTGTTCGTACGTATCGACCGTCGCCGT  >  NC_000913/4181560‑4181850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑