Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 656,251 C→T intergenic (‑283/‑306) dcuC ← / → pagP anaerobic C4‑dicarboxylate transport/phospholipid:lipid A palmitoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913656,2510CT98.7% 271.5 / ‑3.9 75intergenic (‑283/‑306)dcuC/pagPanaerobic C4‑dicarboxylate transport/phospholipid:lipid A palmitoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (39/35);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

TTTTTATACATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGCATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCGCAGAAAACGACGC  >  NC_000913/656104‑656397
                                                                                                                                                   |                                                                                                                                                  
tttttATACATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTAt                                                                                                                                                  >  1:7452/1‑150 (MQ=255)
tttttATACATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTAt                                                                                                                                                  >  1:432591/1‑150 (MQ=255)
 ttttATACATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTAt                                                                                                                                                  >  1:506169/1‑149 (MQ=255)
  tttATACATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAg                                                                                                                                                <  1:987027/150‑1 (MQ=255)
  tttATACATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAg                                                                                                                                                <  1:23524/150‑1 (MQ=255)
     ataCATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGCTGGCGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTg                                                                                                                                            >  2:184687/1‑151 (MQ=255)
     ataCATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGtt                                                                                                                                             <  1:818080/150‑1 (MQ=255)
      taCATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTg                                                                                                                                            >  1:171185/1‑150 (MQ=255)
      taCATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTg                                                                                                                                            >  2:1042652/1‑150 (MQ=255)
        cATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACg                                                                                                                                         >  1:1088991/1‑151 (MQ=255)
         aTAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACg                                                                                                                                         >  2:418320/1‑150 (MQ=255)
             aTAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGatata                                                                                                                                    <  2:759271/151‑1 (MQ=255)
             aTAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGatat                                                                                                                                     >  2:899943/1‑150 (MQ=255)
               aaCAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGatatat                                                                                                                                   >  1:50893/1‑150 (MQ=255)
                aCAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGatatatat                                                                                                                                 >  2:425745/1‑151 (MQ=255)
                 cAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATAtatatt                                                                                                                                <  1:457420/151‑1 (MQ=255)
                  aaaaCGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATAtatatt                                                                                                                                >  2:744396/1‑150 (MQ=255)
                  aaaaCGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGCATAGGTTGACGATAtatatt                                                                                                                                >  2:626325/1‑150 (MQ=255)
                     aCGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATAtatattatat                                                                                                                            <  2:911873/151‑1 (MQ=255)
                           atTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTc                                                                                                                       <  1:796830/150‑1 (MQ=255)
                                     tGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAgatga                                                                                                             <  1:206113/150‑1 (MQ=255)
                                      ggCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGAtt                                                                                                           <  1:519133/151‑1 (MQ=255)
                                         aaTTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGAttgtt                                                                                                        >  1:1048009/1‑151 (MQ=255)
                                          aTTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGAttgttg                                                                                                       <  1:1153017/151‑1 (MQ=255)
                                            tATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATtgttgt                                                                                                      <  2:355130/150‑1 (MQ=255)
                                             aTCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTa                                                                                                     <  2:552279/150‑1 (MQ=255)
                                                  ataataATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATACATATTATATACTTCGTTAAGATGATTGTTGTATCTCGt                                                                                               <  1:937721/151‑1 (MQ=255)
                                                    aataatGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGtt                                                                                              >  1:332055/1‑150 (MQ=255)
                                                     ataatGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTa                                                                                             >  2:496328/1‑150 (MQ=255)
                                                       aatGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTaaa                                                                                           <  1:389707/150‑1 (MQ=255)
                                                           tgtgCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAaaaat                                                                                       >  1:72408/1‑150 (MQ=255)
                                                            gtgCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAaaaataa                                                                                     <  2:34734/151‑1 (MQ=255)
                                                            gtgCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAaaaata                                                                                      <  2:846218/150‑1 (MQ=255)
                                                                  aTTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAaataat                                                                               >  2:1197564/1‑151 (MQ=255)
                                                                     gATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAAttt                                                                             <  2:1120222/150‑1 (MQ=255)
                                                                      aTGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAAttttt                                                                           <  2:20661/151‑1 (MQ=255)
                                                                       tggtggATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAAttttt                                                                           >  1:792946/1‑150 (MQ=255)
                                                                        ggtggATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTc                                                                          <  2:413767/150‑1 (MQ=255)
                                                                        ggtggATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTc                                                                          <  2:521814/150‑1 (MQ=255)
                                                                        ggtggATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTc                                                                          <  2:4518/150‑1 (MQ=255)
                                                                          tggATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCtt                                                                       >  2:566842/1‑151 (MQ=255)
                                                                           ggATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTg                                                                      >  1:494490/1‑151 (MQ=255)
                                                                             aTTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGAt                                                                    <  1:385295/151‑1 (MQ=255)
                                                                             aTTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGAt                                                                    <  2:1172511/151‑1 (MQ=255)
                                                                                gATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGc                                                                 <  1:1080477/151‑1 (MQ=255)
                                                                                     aaaTTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCAtttt                                                            <  2:952439/151‑1 (MQ=255)
                                                                                     aaaTTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCAtttt                                                            >  2:259810/1‑151 (MQ=255)
                                                                                      aaTTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAAGATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGCTTGCATtgtg                                                           <  1:210598/151‑1 (MQ=255)
                                                                                       aTTTGTTAATTGCAATGGTGTTAGCTTCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGt                                                          <  2:371739/151‑1 (MQ=255)
                                                                                       aTTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGt                                                          <  1:906251/151‑1 (MQ=255)
                                                                                         ttGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGtc                                                         <  2:850776/150‑1 (MQ=255)
                                                                                          tGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGtca                                                        <  2:605843/150‑1 (MQ=255)
                                                                                           gTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGtcat                                                       <  2:757962/150‑1 (MQ=255)
                                                                                               aTTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCaaa                                                   >  2:160630/1‑150 (MQ=255)
                                                                                                    aaTGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGa                                              >  1:57054/1‑150 (MQ=255)
                                                                                                     aTGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACt                                            >  2:858303/1‑151 (MQ=255)
                                                                                                               cTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCt                                  >  1:934484/1‑151 (MQ=255)
                                                                                                                 gCGGCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCttt                                >  2:55328/1‑151 (MQ=255)
                                                                                                                    gCTGTGCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCtttttt                             >  2:456326/1‑151 (MQ=255)
                                                                                                                       gtgCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTGCTTTTTTg                            <  1:859086/149‑1 (MQ=255)
                                                                                                                        tgCGCTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACt                         >  2:713428/1‑151 (MQ=255)
                                                                                                                         gcgcTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTa                        <  1:1064059/151‑1 (MQ=255)
                                                                                                                           gcTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTAtt                      >  1:241062/1‑151 (MQ=255)
                                                                                                                            cTCAAAAAATAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGATTTTTCTTTTTTGACTATTc                     >  1:600181/1‑151 (MQ=255)
                                                                                                                               aaaaaaTAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTccc                   >  1:433779/1‑150 (MQ=255)
                                                                                                                                 aaaaTAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATc                <  2:293571/151‑1 (MQ=255)
                                                                                                                                  aaaTAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCg               >  1:640808/1‑151 (MQ=255)
                                                                                                                                  aaaTAATCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCg               >  1:416933/1‑151 (MQ=255)
                                                                                                                                     taatCTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCGc              >  1:930490/1‑149 (MQ=255)
                                                                                                                                         cTAATATGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCGCAGaaa         >  1:710205/1‑150 (MQ=255)
                                                                                                                                            atatGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCGCAGAAAacg      >  1:827654/1‑150 (MQ=255)
                                                                                                                                              atGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCGCAGAAAacgacg   <  1:624369/151‑1 (MQ=255)
                                                                                                                                               tGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCGCAGAAAACGACGc  >  1:635402/1‑151 (MQ=255)
                                                                                                                                               tGAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCGCAGAAAACGACGc  >  2:508917/1‑151 (MQ=255)
                                                                                                                                                gAGTATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCGCAGAAAACGACGc  >  1:154308/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTTTATACATAGATAACAAAACGCATATTTAGCGGTTGGCAATTATCACATAATAATGTGTGCAAATTGATGGTGGATTGATGCAAATTTGTTAATTGCAATGGTGTTAGCTGCGGCTGTGCGCTCAAAAAATAATCTAATATGAGCATAGGTTGACGATATATATTATATACTTCGTTAAGATGATTGTTGTATCTCGTTAAAAAATAAAATAATTTTTCCTTGATTGCATTTTGTCATCAAAAAAGACTTGGTTTTTCTTTTTTGACTATTCCCATCGCAGAAAACGACGC  >  NC_000913/656104‑656397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: