Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,198,201 C→T E13K (GAA→AAA)  ymfE ← e14 prophage; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,198,2010CT94.7% 253.0 / 4.4 75E13K (GAA→AAA) ymfEe14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base T (38/33);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAG  >  NC_000913/1198054‑1198343
                                                                                                                                                   |                                                                                                                                               
cccAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTAtt                                                                                                                                              <  1:960993/151‑1 (MQ=255)
cccAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCAtt                                                                                                                                              <  1:1166339/151‑1 (MQ=255)
 ccAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTttatta                                                                                                                                             <  1:1184574/151‑1 (MQ=255)
   aaTTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATtata                                                                                                                                           <  2:615006/151‑1 (MQ=255)
    aTTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATtata                                                                                                                                           >  2:728734/1‑150 (MQ=255)
       aataaAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAttt                                                                                                                                       >  2:578789/1‑151 (MQ=255)
       aataaAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAtt                                                                                                                                        <  1:889358/150‑1 (MQ=255)
        ataaAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAtttt                                                                                                                                      <  1:221416/151‑1 (MQ=255)
          aaaGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTg                                                                                                                                   >  1:736516/1‑151 (MQ=255)
            aGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTggg                                                                                                                                 <  2:789437/151‑1 (MQ=255)
             gCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTgggg                                                                                                                                <  2:858800/151‑1 (MQ=255)
               tctcATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTgggggg                                                                                                                              >  2:837367/1‑151 (MQ=255)
                  cATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGt                                                                                                                             <  1:359209/149‑1 (MQ=255)
                         tttAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACat                                                                                                                     >  1:533543/1‑150 (MQ=255)
                           tAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACatat                                                                                                                   <  1:816902/150‑1 (MQ=255)
                            aaTAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACAtatta                                                                                                                 <  2:51761/151‑1 (MQ=255)
                            aaTAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACAtatt                                                                                                                  >  1:712560/1‑150 (MQ=255)
                               aGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTc                                                                                                              >  2:190687/1‑151 (MQ=255)
                                   gggACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCt                                                                                                          >  2:684464/1‑151 (MQ=255)
                                   gggACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCt                                                                                                          >  1:69498/1‑151 (MQ=255)
                                       cATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGt                                                                                                      >  1:914686/1‑151 (MQ=255)
                                         tACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTc                                                                                                     >  2:852691/1‑150 (MQ=255)
                                          aCTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCa                                                                                                    <  2:391627/150‑1 (MQ=255)
                                           cTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACg                                                                                                  >  2:1096096/1‑151 (MQ=255)
                                                        gTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAgt                                                                                      <  1:561022/150‑1 (MQ=255)
                                                          tattaAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAgtgt                                                                                    <  1:303911/150‑1 (MQ=255)
                                                             taAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAAt                                                                                 <  1:847931/150‑1 (MQ=255)
                                                              aaaGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATgg                                                                               >  2:176836/1‑151 (MQ=255)
                                                              aaaGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATCATTCACCTGAGTCACGCTTTAGGATAGTGTAATgg                                                                               >  1:582433/1‑151 (MQ=255)
                                                                      gTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATAttt                                                                       <  2:506088/151‑1 (MQ=255)
                                                                      gTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATAtt                                                                        <  2:1121889/150‑1 (MQ=255)
                                                                      gTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATAtt                                                                        <  2:1173384/150‑1 (MQ=255)
                                                                        aTTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATtta                                                                      >  1:1083745/1‑150 (MQ=255)
                                                                           cGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaatta                                                                  <  2:531807/151‑1 (MQ=255)
                                                                           cGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaatt                                                                   >  2:603496/1‑150 (MQ=255)
                                                                           cGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaatt                                                                   >  1:1007992/1‑150 (MQ=255)
                                                                            gTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaattaa                                                                 <  2:281675/151‑1 (MQ=255)
                                                                            gTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaatta                                                                  >  2:794100/1‑150 (MQ=255)
                                                                                    cTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCAtt                                                         >  1:647929/1‑151 (MQ=255)
                                                                                      aGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCAtt                                                         <  2:484211/149‑1 (MQ=255)
                                                                                      aGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCAtt                                                         <  2:170290/149‑1 (MQ=255)
                                                                                      aGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATtat                                                       >  2:693499/1‑151 (MQ=255)
                                                                                      aGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATta                                                        <  1:129770/150‑1 (MQ=255)
                                                                                      aGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATta                                                        <  2:1021743/150‑1 (MQ=255)
                                                                                        aaGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATtatat                                                     >  2:1216668/1‑151 (MQ=255)
                                                                                        aaGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATtatat                                                     >  1:888240/1‑151 (MQ=255)
                                                                                             tGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACttt                                                >  1:357592/1‑151 (MQ=255)
                                                                                             tGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACttt                                                >  2:612313/1‑151 (MQ=255)
                                                                                               cAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTc                                              >  2:39406/1‑151 (MQ=255)
                                                                                               cAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTc                                              >  2:916841/1‑151 (MQ=255)
                                                                                                     atTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGCTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTa                                          >  2:38716/1‑149 (MQ=255)
                                                                                                       tAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgtt                                      >  1:176025/1‑151 (MQ=255)
                                                                                                        aGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATTCTTTTCATTAGgtt                                      <  2:157459/150‑1 (MQ=255)
                                                                                                          cGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaatt                                                                   >  2:857427/1‑119 (MQ=255)
                                                                                                          cGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaatt                                                                   <  1:857379/119‑1 (MQ=255)
                                                                                                           gTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgttagtt                                  <  1:568877/151‑1 (MQ=255)
                                                                                                            tATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgttagtt                                  >  2:457260/1‑150 (MQ=255)
                                                                                                             aTTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgttagtta                                 <  2:648431/150‑1 (MQ=255)
                                                                                                               ttAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACa                               >  2:586934/1‑150 (MQ=255)
                                                                                                                 aaGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAAtt                            <  2:294031/151‑1 (MQ=255)
                                                                                                                    aaaGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAAttttt                         <  2:259274/151‑1 (MQ=255)
                                                                                                                     aaGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGATAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAAtttttt                        >  1:1170162/1‑151 (MQ=255)
                                                                                                                      aGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTg                       <  2:698681/151‑1 (MQ=255)
                                                                                                                        ttGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGt                      >  2:191577/1‑150 (MQ=255)
                                                                                                                               aTGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGtt               <  1:532075/150‑1 (MQ=255)
                                                                                                                                tGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTc              >  2:670801/1‑150 (MQ=255)
                                                                                                                                 gAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTc              >  1:1133267/1‑150 (MQ=255)
                                                                                                                                  aaGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAgg           <  1:162255/151‑1 (MQ=255)
                                                                                                                                    gTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCt         <  1:663761/151‑1 (MQ=255)
                                                                                                                                    gTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCt         <  2:9833/151‑1 (MQ=255)
                                                                                                                                        aGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGAcc     <  1:237990/151‑1 (MQ=255)
                                                                                                                                        aGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGAcc     >  1:613416/1‑151 (MQ=255)
                                                                                                                                         ggCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGGTAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCCGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCt    >  2:685788/1‑151 (MQ=255)
                                                                                                                                          gCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTa   <  2:300854/151‑1 (MQ=255)
                                                                                                                                           cAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCt    >  2:440113/1‑149 (MQ=255)
                                                                                                                                            aaCATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAg  >  1:1191073/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAG  >  NC_000913/1198054‑1198343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: