Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,764,785	0	T	A	43.0%	112.8 / 46.4	79	intergenic (‑399/+149)	sufA/ydiH	Fe‑S cluster assembly protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (39/6);  new base A (0/34);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.74e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCATTAAGTCAGGATC  >  NC_000913/1764636‑1764935                                                                                                                                                         |                                                                                                                                                       ttAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCAt                                                                                                                                                        >  1:808194/1‑150 (MQ=255) ttAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCAt                                                                                                                                                        >  1:523314/1‑150 (MQ=255)  tAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATa                                                                                                                                                       >  2:548859/1‑150 (MQ=255)   aaGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATaaa                                                                                                                                                     <  1:1077863/151‑1 (MQ=255)   aaGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATaa                                                                                                                                                      >  2:932487/1‑150 (MQ=255)    agagATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATaa                                                                                                                                                      >  2:74637/1‑149 (MQ=255)    agagATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATaa                                                                                                                                                      >  1:412213/1‑149 (MQ=255)      agaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCt                                                                                                                                                  >  2:67297/1‑151 (MQ=255)      agaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCt                                                                                                                                                  >  1:886089/1‑151 (MQ=255)      agaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCt                                                                                                                                                  <  2:919462/151‑1 (MQ=255)       gaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CACCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGACGGGGCGATAAGAATAATCTCATAAAGCt                                                                                                                                                  >  1:348178/1‑150 (MQ=255)        aTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTa                                                                                                                                                 >  2:219443/1‑150 (MQ=255)         tAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAc                                                                                                                                               >  1:1036474/1‑151 (MQ=255)           ggTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAccc                                                                                                                                             <  2:558795/151‑1 (MQ=255)                aaaaGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGt                                                                                                                                        >  2:697176/1‑151 (MQ=255)                aaaaGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGt                                                                                                                                        >  2:747711/1‑151 (MQ=255)                aaaaGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGt                                                                                                                                        >  1:319131/1‑151 (MQ=255)                 aaaGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGtt                                                                                                                                       >  2:375495/1‑151 (MQ=255)                  aaGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCGGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCAAAAAGCTAACCCGCCGttt                                                                                                                                      <  1:1092127/151‑1 (MQ=255)                   aGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGtttt                                                                                                                                     >  1:651927/1‑151 (MQ=255)                    gtccggCGTTCAAAAAAAAGAAACCCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑CCCC‑CCCCACCCGGCTTTTATCCCCGCCCCTCTGAGTGTTTTGCCTTTTGAGGGGGCGAAAAGAATAATCTCAAAAAGCAAACCCGCCGtttt                                                                                                                                     <  1:3245/145‑1 (MQ=255)                      gCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAac                                                                                                                                  >  1:687886/1‑151 (MQ=255)                       cAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAac                                                                                                                                  >  2:393920/1‑150 (MQ=255)                        aGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAacac                                                                                                                                >  2:730908/1‑151 (MQ=255)                            ttCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAAc                                                                                                                            >  2:347607/1‑151 (MQ=255)                              cAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTg                                                                                                                          >  1:147045/1‑151 (MQ=255)                              cAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTg                                                                                                                          >  1:51173/1‑151 (MQ=255)                              aaaaaaaaaaaaaCCCC‑TTAAGTCGGGGCGACACGGGTTTAAGGGTT‑TCTC‑CCCCACCCGGCGCTTATCCCCGCCACTCTCAGGGGTTTAGCTCTGGAGGGGGCGATAAAAATAATCTCAAAAAGCAACCCCGCCGTTTTAACACAAACTg                                                                                                                          <  2:869249/148‑1 (MQ=255)                               aaaaaaaaaaaaCCCC‑AAAATTCTGTGCGACAC‑GGTTTAAGGGTTCTCCC‑CCCCATCGGGCGTTTATCCCCGGCACTCTAAGTGGTTTGGCTTTAAAGGGGGCAAAAAAAATAATCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTg                                                                                                                          <  2:393129/148‑1 (MQ=255)                                    tAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTGTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAtt                                                                                                                     >  1:904556/1‑150 (MQ=255)                                     aaaaaacCCC‑TTAAGTCTGTGCGACACAGGCTTAAGGTTT‑TCTC‑CCCCACCCGGCGCTTATCCCCGGCACTCTCAGTGGCTTAGCTCTTGAGGGGGCGATAAAAATAATCTCAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAg                                                                                                                   <  2:1013353/148‑1 (MQ=255)                                     aaGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGAtt                                                                                                                     >  2:302574/1‑149 (MQ=255)                                      aaaaaaCCCTTTAGGTGTGGGCGCCACGGGCTTAGGGGTT‑TCCC‑CCCCCCCCGGCTCTTCTCTCCGCCACTCTCGGTGCTTTCGCTCTTGAAGGGTCAAAAAGAATAATCTGATAAGGTTACCCCGCCGTTTTAACACAAACTGCGAt                                                                                                                      <  2:773331/146‑1 (MQ=255)                                      aGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAg                                                                                                                   >  2:1101915/1‑150 (MQ=255)                                      aGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGt                                                                                                                  >  1:1053524/1‑151 (MQ=255)                                        aaacCCC‑TTAAGTCTGTGCGACACAGGCTTAAGGTTT‑TCTC‑CCCCACCCGGCGTTTATCTCCGGCACTCTCAGGGGTTTAGCTTTTGAAGGGGCGAAAAAAATAATCTCAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGta                                                                                                                 <  2:612145/150‑1 (MQ=255)                                        aaaaCCCTTTAAGTCTGTGCGACACAGGTTTAAGGTTT‑TCTC‑CCCCACCCGGCTCTTATCCCCGCCACTCTCAGTGGCTTAGCTTTTGAAGGGGAGATAAAAATAATCTAAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGta                                                                                                                 <  1:1030414/151‑1 (MQ=255)                                         aaaCCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtatt                                                                                                               >  2:1120963/1‑151 (MQ=255)                                             ccctaagctCTGGGCGACCGAGACTAAGGGTTT‑TCCC‑CCCCCCCCGGCGCTTACCCCCGGCCCCCTCAGTGGTTTAGCTTTTGAAGGGCGAATAAAAATAATCTAAAAAAGCCACCCCGCCGTTTTAACACAAACTGCGATTAGtattat                                                                                                             <  1:9348/142‑1 (MQ=255)                                             cc‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTAttt                                                                                                           >  2:294044/1‑151 (MQ=255)                                                taaaGTCTGTGCGACACAGGCTAAAGGTTT‑TCCC‑CCCCACCCGGCTCTTATCCCCGCCACCCTAAGTGGTTTAGCTTTTGAAGGGGCAAAAAAAATAATCTAAAAAACCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTAtttt                                                                                                          <  1:1103925/148‑1 (MQ=255)                                                  agtccTGTGCGCCACAGGCTAAGGGTTT‑TCTC‑CCCCACCGGGCGTTTATCCCCGCCACTCTCAGTGGTTTAGCTCTTGAGGGGGCAAAAAAAATAATCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTg                                                                                                        <  2:5958/146‑1 (MQ=255)                                                  aattcggtgCCACACGGGTTAAAGGGTT‑TCAC‑CCCCCCCGGGCGTTTATCCCCGCCCCTCTCAGTGCTTTTTCTTTTAAGGGGGAAATAAAAATATTCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTg                                                                                                        <  1:983065/144‑1 (MQ=255)                                                  aaGTCTGTGCGACACAGGTTTAAGGTTT‑TCTC‑CCCCATCCGGCGCTTATCTCCGCCACTCTCAGGGGCTTAGCTCTTGAAGGGGCAATAAGAATAATCTCAAAAATCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGa                                                                                                       <  2:815812/151‑1 (MQ=255)                                                        gtgCGACCCAGGCTTAGGGGTT‑TCTA‑CCCCATCGGGCGCTTATCCCCGGCACTCTCAGTGTTTTAGCTTTGGAAGGGGCGATAAAAATAATCTAATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAata                                                                                                 <  2:586486/151‑1 (MQ=255)                                                        gtgCGACACAGGCTTAAGGTTT‑TCTC‑CCCCCCCCGGCGCTTATCTCCGGCCCTCTCAGTGGTTTAGCTTTTGAGGGGGCGATAAGAATAATCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAat                                                                                                  <  2:151041/150‑1 (MQ=255)                                                        gtgCGACACAGGCTAAAGGTTT‑TCTC‑CCCCACCCGGCGCTTACCTCCGGCCCCCCCAGTGGTTTGGCTTTTGAAGGGGAAAAAAGAATAATTTAAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGaa                                                                                                      <  1:147844/146‑1 (MQ=255)                                                           cGACACGGCTTTAAGGGTT‑TCTC‑CCCCACCGGGCTTTTACCTCCGCCTCCCCCAGTGGCTAAGTTCTTGAGGGGGCGAAAAAAATAATCTCAAAAAGTTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATc                                                                                               <  2:515107/150‑1 (MQ=255)                                                           cGACACATGATT‑AGGGTT‑TCCACCCCCCCCCGCCGCTTATCCCCGGCACTCCCAGTGTTTTGGCTTTTAAAGGGGAAATAAAAATAATCTAAAAAAGCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCa                                                                                              <  1:76653/151‑1 (MQ=255)                                                            gACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATc                                                                                               >  2:635310/1‑149 (MQ=255)                                                            gACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATc                                                                                               >  2:670168/1‑149 (MQ=255)                                                             acacAGTCTTAAGGTTT‑CCTC‑CCCCACCCGGCTCTTATCCCCGCCCCTCTCAGTGCCTTACCTCTTGAGGGGGCAAAAAAAATAATCTCAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACa                                                                                                    <  2:1023110/143‑1 (MQ=255)                                                              cacggggtaaaGGGTT‑TCTC‑CCCCATCGGGCTTTTATCCCCGCCTCTTTAAGGGCTTTGGCTTTTAAGGGGTCAAAAAGAATAATCCCAAAAAGTTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACaa                                                                                                   <  2:716043/134‑1 (MQ=255)                                                               acagtttaaaGGGTT‑TCTC‑CCCCACCCGGCTCTTATCTCCGCCACTCTCAGTGGTTTAGCTTTTGAAGGGGCAAAAAAAATAATCAAAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAgg                                                                                            <  1:141641/141‑1 (MQ=255)                                                                caGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCgg                                                                                         >  2:625038/1‑151 (MQ=255)                                                                 aGGTTTAGGGTTT‑TCTC‑CCCCACCCGGCGCTTATCCCCGGCCCTCTCAGTGGTTTAGCTTTTGAAGGGGCAAAAAAAATAATCTAAAAAAGCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCg                                                                                          <  1:558972/149‑1 (MQ=255)                                                                  ggCTTAAGGGTT‑TCTC‑CCCCACCCGCCGCTTTTCTCCGCCCCTCTCAGTGGTTTAGCTCTTGAAGGGGCGATAAAAAAAATCTAAAAAAGTTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCg                                                                                          <  2:47222/148‑1 (MQ=255)                                                                     ttAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGt                                                                                        >  2:307439/1‑147 (MQ=255)                                                                       aaGGTTT‑TCTC‑CCCCACCCGCCCTTTATCTCCGCCCCTCTCGGTGGTTTAGCTTTGGAAGGGGAAATAAAAATAATCTAAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATaa                                                                                  <  1:28512/151‑1 (MQ=255)                                                                         agggttttcccccccCTCCGGCGTTTATCTCCGCCACTCTCAGTGGCTTAGCTTTTGAAGGGGAAAAAAAAATAATCTCATAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGa                                                                                     <  2:796364/137‑1 (MQ=255)                                                                          gttt‑tCTC‑CCCCACCCGGCCCTTATACCCGCCACTCTCGGTGGTTTAGTTTTGGAGGGGGGAAAAAAAAAAATCTAAAAAGGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCa                                                                               <  2:1061853/149‑1 (MQ=255)                                                                             t‑tCTC‑CCCCACCCGGCCTTTACCCCCGCCCCTCTCAGTGGTTTATCTTTTAAGGGGGCAAAAAAAAAAATTTAAAAAAGCAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGt                                                                             <  1:1155623/150‑1 (MQ=255)                                                                             t‑tCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGAAGt                                                                             >  1:777282/1‑150 (MQ=255)                                                                                  a‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTa                                                                         >  1:1117828/1‑150 (MQ=255)                                                                                    ccccACCCGGCGCTTCTCCCCGGCCCTCTCATTGGTTTAGCTCTTAAGGGGGCGAAAAGAATAATCTAAAAAAGCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGt                                                                             <  2:1202615/145‑1 (MQ=255)                                                                                       cATCCGGCGTTTATCCCCGGCCCTCTCAGTGGTTTAGCTTTTAAAGGGGAGATAAAAATAATCTAAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTaagaag                                                                    <  1:263881/151‑1 (MQ=255)                                                                                         cccGGCGCTTGCCTCGGCCCCTCTCAGTGATTTAGCTTTTGAGGGGGAAAAAAAAAAAATCCAAAAACTCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGt                                                                  <  1:46332/150‑1 (MQ=255)                                                                                                               ctcAGTGGTTTAGCTCTAAAGGGGGAAATAAAAATAATCAAAAAAAGAAAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGt                                              <  1:471022/149‑1 (MQ=255)                                                                                                                  aGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATc                                         >  1:274062/1‑151 (MQ=255)                                                                                                                  aGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATc                                         >  1:905437/1‑151 (MQ=255)                                                                                                                          acgtctTGAAGGGGCGACAAGAATAATCTCATAAAGCTAACGCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGCAGATAATCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCg                                  >  1:329179/4‑150 (MQ=255)                                                                                                                          aGCTTTTGAGGGGGAAATAAAAATAATCTCAAAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCg                                  <  2:952596/150‑1 (MQ=255)                                                                                                                             ttttGAGGGGGCAAAAAAAATAATCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGAtt                               <  2:886134/148‑1 (MQ=255)                                                                                                                                tGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGc                           >  2:422702/1‑151 (MQ=255)                                                                                                                                    ggggCAAAAAAAATAATCTAAAAAATATAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAAc                       <  1:747681/151‑1 (MQ=255)                                                                                                                                     gggCGAAAAAAATAACCTAAAAAAAATACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACt                      <  2:1036534/151‑1 (MQ=255)                                                                                                                                     gggAGATAAGAATAATCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACt                      <  1:295125/151‑1 (MQ=255)                                                                                                                                      ggcaaaaaaaataatCTAAAAAAGCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACtt                     <  2:891966/142‑1 (MQ=255)                                                                                                                                         gATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCg                  >  1:722459/1‑151 (MQ=255)                                                                                                                                                     ctcATAAAGCTAACCCGCCGTTTTAACACAAACTGCGCTTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCATTAAGTc        >  2:643702/1‑149 (MQ=255)                                                                                                                                                      tcATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCATTAAGTCAgg     >  1:1004327/1‑151 (MQ=255)                                                                                                                                                         tAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCATTAAGTCAGGATc  >  2:181687/1‑151 (MQ=255)                                                                                                                                                         |                                                                                                                                                       TTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCC‑TTAAGTCTGTGCGACACAGGCTTAAGGGTT‑TCTA‑CCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATAAGCAGTATTAAGAAGGTCATCGAACCTGGACGGAGGTTAATCCAGGTCGATTTGGCGAACTTGCGGCATTAAGTCAGGATC  >  NC_000913/1764636‑1764935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑