Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	5,159	0	G	A	41.7%	63.4 / 39.1	60	intergenic (+139/‑75)	thrC/yaaX	L‑threonine synthase/DUF2502 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (30/5);  new base A (0/25);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 5.49e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAA  >  NC_000913/5012‑5308                                                                                                                                                       |                                                                                                                                                       catcaGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAAt                                                                                                                                                     <  1:813329/151‑1 (MQ=255)      gTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATaacaa                                                                                                                                                <  1:406542/151‑1 (MQ=255)        aaaaTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑cc                                                                                                                                             <  2:161560/151‑1 (MQ=255)           aTCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑ccgcc                                                                                                                                          >  2:93728/1‑151 (MQ=255)               aTTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGTTTTTTTATG‑AAGAAATTATGGAAAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAAAATAACAA‑ccgccg                                                                                                                                         <  1:362081/148‑1 (MQ=255)                ttcttttacaaaaTCAGGCGGGGTTGGCTTTTATGCAGCCGGGTTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTAAATAAATGCGGTAACTGGAAGATTAGGA‑TTGGGGAGAAAAACAA‑ccgccg                                                                                                                                         <  1:83890/136‑1 (MQ=255)                ttCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTtct                                                                                                                                     >  2:858149/1‑151 (MQ=255)                      aTCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCg                                                                                                                                >  2:867094/1‑150 (MQ=255)                        ctcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAg                                                                                                                              >  1:835923/1‑150 (MQ=255)                         tcAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGt                                                                                                                             >  1:363326/1‑150 (MQ=255)                          cAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTa                                                                                                                            >  1:720498/1‑150 (MQ=255)                             tCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAAtct                                                                                                                        >  1:516678/1‑151 (MQ=255)                              cAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAAtctc                                                                                                                       >  1:172419/1‑151 (MQ=255)                                           tttttATGCGGCCCTTTTTTTTTATA‑AAAAAATTATGGAAAAAAAGGACAGGAAAAAAGG‑AAAAATTCTCAATAAATGCGTTAATTTAGAGATTAGGA‑GTGCGGAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGa                                                                                                             <  1:48945/147‑1 (MQ=255)                                            ttttACGCCCCCCGGTTTTTTTAAA‑AAAAAATTAAGAAAAAAAATAACAGGAAAAAAGG‑AAAAATTCTAAAAAAATTGGGAAACTTAGATATTAGGA‑TTGGGGAAAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGAcc                                                                                                           <  2:1211387/149‑1 (MQ=255)                                              ttAGGCACCCCGGTTTTTTTAAA‑AAAAAATTAGGAAAAAAAAGGAAAGGAAAAAAGA‑AAAAATTCTAAAAAAATGCGGAAATTAAGAGATTAGGA‑TGGGGGAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCAt                                                                                                        <  2:416961/150‑1 (MQ=255)                                               tattccccccGGTTTTTTTATA‑AAAAAATTAGGGAAAAAAAGGACAGGCAAAAAGT‑AAAAATTCTAAAAAAGCGCGGAAACTTAGAGA‑TAGAACTGGAAAAAAAAAACAC‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCAt                                                                                                        <  2:77161/142‑1 (MQ=255)                                                 tGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACg                                                                                                    >  1:635364/1‑151 (MQ=255)                                                     gCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGc                                                                                                 >  2:1164883/1‑150 (MQ=255)                                                     ccccGGTTTTTTAAAA‑AAAAAAGTA‑GGAAAAAAAAGAAAAGGAAAAAGGAAAAAATACAAAAAAAATGCGTTAATTAAGAAATGAGGT‑TGGGGAAAAAAAACAG‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCa                                                                                                <  1:815300/150‑1 (MQ=255)                                                      ccccgtttttttAAGAAAGAAATAATAGAAAAAAAGGAAAGAAAAAAAGG‑AGAAATTCTCAATAAATGCGTTAATTTAGAGATAAGGT‑TTGGGAAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACggg                                                                                                  <  2:892345/143‑1 (MQ=255)                                                       cggtttttttaATA‑AAAAAATTATGAAAAAAAAGGAAAGGAAAAAAGA‑AAAAATTCTAAAAAATTGCGATAACGAAGAGATTAGGT‑TGGGGGAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCa                                                                                                <  1:1124508/144‑1 (MQ=255)                                                          gtttttttAAGAAAAAAATTATGGAAAAAAATGAAAGGAAAAAAGG‑AAAAATTCAAAATAAAGGCGTTATTTTAGAATTTAGGT‑TTGGGGAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGa                                                                                            <  1:213221/149‑1 (MQ=255)                                                           tttttttATA‑AAAAAATTAAGGAAAAAAATGACAGGAAAAAAGA‑AAAAATTCTAAATAAATGTGGTAATTTAGAAATTAGTA‑TGGCGAAAAAAAAAAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGa                                                                                            <  2:1170991/148‑1 (MQ=255)                                                           cTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGAt                                                                                           >  1:937772/1‑150 (MQ=255)                                                             tttttAAA‑AAAAAATTAGGGAAAAAAAAGAAAGAAAAAAAGG‑AAAATTCCTAAAAAAGTGCGTTAACTAAGATATTAGGA‑GGGCGGAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaa                                                                                         <  1:305806/150‑1 (MQ=255)                                                              ttttATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaa                                                                                        >  2:726848/1‑150 (MQ=255)                                                                   ataaaaaaaTTAGGGAAAAAAAGGAAAGGAAAAAAGA‑AAAAATTCAAAAAAAATGCGTTAATTTAGAATTTAGGA‑TTGGGGAAAAAAAAAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAg                                                                                      <  1:128883/142‑1 (MQ=255)                                                                      aagaaATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTa                                                                                 >  2:276394/1‑150 (MQ=255)                                                                       agaaTTGATGGAAAAAAATGAGAGGGAAAAAGG‑AGAATCACAAAATAAAGGGGCTACCTTGGGGATGGGGA‑TGGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt                                                                                  <  2:476133/148‑1 (MQ=255)                                                                        aaatttATGAAAAAAAAAGACAGGAAAAAAGA‑AAAATTTCTAAAAAAAGGCGATATTTAAGGAATGAGGT‑TGGGGGAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAAcc                                                                              <  2:611941/147‑1 (MQ=255)                                                                          aaTTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtg                                                                            >  2:182468/1‑151 (MQ=255)                                                                          aaTTAGGAAAAAAAAGGAGAGAAAAAAAGA‑AAAAATTCAAAAAAAGGGCGGTAATTAAGAGATAGGGG‑TGGGGAAAAAAAAAAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtg                                                                            <  1:731581/151‑1 (MQ=255)                                                                                  aaaaaaaGGACAGGAAAAAAGA‑AAAAATTCAAAAAAAAGGCGATAATTTAGAGATT‑GGA‑ATGGGAAAAAAAAAAACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtg                                                                            <  1:19283/141‑1 (MQ=255)                                                                                         tGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAAt                                                             >  2:1058709/1‑151 (MQ=255)                                                                                            cAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCt                                                           >  2:430641/1‑150 (MQ=255)                                                                                             aGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTAt                                                         >  2:1086870/1‑151 (MQ=255)                                                                                             aGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGAAGTAACCTGTGAAAAAGATGCAATCTAt                                                         >  1:101547/1‑151 (MQ=255)                                                                                              ggaaaaaaGG‑AGAAATTCGCAAAAATGGTGGTAGTTTAGAATTTAGGA‑TTGGGGAAAATAATAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAAt                                                             <  2:445028/143‑1 (MQ=255)                                                                                                   aaaGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACt                                                   >  1:293854/1‑151 (MQ=255)                                                                                                    aggg‑AAAAATTCTAAATAAAGGCGTTATTTTAGAGATTAGGA‑TTGCGGAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTc                                                  <  2:191684/149‑1 (MQ=255)                                                                                                    aagaaagaAATCCGAAATAAATGCGGTACGTTAGATATGGGGA‑AGGGAGAAAATAACAG‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTa                                                     <  2:299600/144‑1 (MQ=255)                                                                                                     aagaaaaaaTTCTAAAAAAATGGGGTAACTAAGAGATTAGGA‑TTGCGGAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTc                                                  <  2:364166/145‑1 (MQ=255)                                                                                                      gg‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGc                                                >  1:1178467/1‑151 (MQ=255)                                                                                                       gaaaaaaTTCAAAAAAAATGGGGTAATTAAGAGATTAGGT‑TGGCGAAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCg                                                 <  2:262420/146‑1 (MQ=255)                                                                                                       g‑agaAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGc                                                >  2:886093/1‑150 (MQ=255)                                                                                                       g‑agaAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGc                                                >  2:519770/1‑150 (MQ=255)                                                                                                           aaaTTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACtt                                            >  1:480908/1‑151 (MQ=255)                                                                                                           aaaTTCAAAATAAATGCGTTAATTAAGAAATTAGGA‑TGGCGAAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACtt                                            <  1:288550/151‑1 (MQ=255)                                                                                                              ttCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCAGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTcc                                         >  1:503073/1‑151 (MQ=255)                                                                                                                    aTAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGtt                                   >  1:285027/1‑151 (MQ=255)                                                                                                                      aaagggggaaattaagagATTAGGA‑TGGCGGAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCt                                 <  1:616033/137‑1 (MQ=255)                                                                                                                        ctGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCCCCGTTATCACCTAGTAATCAGCGGATATCTACCCATAACGGACAATGATCTATTGAGTAACCTGTGAAATAGATGCAATCTATCGTCCTCGCACTTTCCCTGGTTCCgg                               >  1:924635/2‑151 (MQ=255)                                                                                                                         tGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGt                              >  1:564427/1‑151 (MQ=255)                                                                                                                          gggttaattaagagATGAGGA‑TGGCGAAAAAAAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTc                             <  2:307315/141‑1 (MQ=255)                                                                                                                            ggaaataaaggaaTTAGGT‑TTGCGAAAAATAAAAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCg                            <  2:152551/138‑1 (MQ=255)                                                                                                                                         ttAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAATCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgcag                >  2:895734/1‑149 (MQ=255)                                                                                                                                         ttAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGgcagc               >  2:656834/1‑150 (MQ=255)                                                                                                                                            ggA‑TTGGGAAAAAAAAAAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAg           <  2:139780/151‑1 (MQ=255)                                                                                                                                                  gCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGc      >  2:28173/1‑151 (MQ=255)                                                                                                                                                       gaATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGaa  >  1:332609/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                       CATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATG‑AAGAAATTATGGAGAAAAATGACAGGGAAAAAGG‑AGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGA‑TTGCGGAGAATAACAA‑CCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAA  >  NC_000913/5012‑5308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑