Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 82,372 T→C E386E (GAA→GAG leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091382,3720TC98.9% 330.1 / ‑4.4 87E386E (GAA→GAGleuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (40/46);  total (40/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01

TGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTC  >  NC_000913/82229‑82519
                                                                                                                                               |                                                                                                                                                   
tGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTtgct                                                                                                                                              >  2:425525/1‑151 (MQ=255)
 gATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTtgct                                                                                                                                              <  2:123616/150‑1 (MQ=255)
  aTAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTtgctg                                                                                                                                             >  2:1126536/1‑150 (MQ=255)
    aGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCtt                                                                                                                                          >  2:675706/1‑151 (MQ=255)
       cGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTAcc                                                                                                                                       <  1:34549/151‑1 (MQ=255)
           aTCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATg                                                                                                                                   <  1:1245787/151‑1 (MQ=255)
              gaccggaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGa                                                                                                                                  >  2:848008/1‑149 (MQ=255)
              gaccggaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAg                                                                                                                                <  1:519439/151‑1 (MQ=255)
                ccggaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAgg                                                                                                                               <  2:739354/150‑1 (MQ=255)
                  ggaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGGAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGcc                                                                                                                             >  1:1095616/1‑150 (MQ=255)
                   gaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGcc                                                                                                                             >  2:368198/1‑149 (MQ=255)
                     ccgttaccgttGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAgcg                                                                                                                         >  2:841642/1‑151 (MQ=255)
                      cgttaccgttGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAgcgc                                                                                                                        <  1:1205201/151‑1 (MQ=255)
                      cgttaccgttGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAgcgc                                                                                                                        >  1:383869/1‑151 (MQ=255)
                         taccgttGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCt                                                                                                                      <  2:347707/150‑1 (MQ=255)
                                ggcggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATc                                                                                                               <  2:19652/150‑1 (MQ=255)
                                ggcggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCg                                                                                                              <  1:300656/151‑1 (MQ=255)
                                 gcggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGt                                                                                                             >  2:1194654/1‑151 (MQ=255)
                                 gcggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGAATGCACGCTGAAGGAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGt                                                                                                             >  1:1065076/1‑151 (MQ=255)
                                      ttCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATc                                                                                                         >  1:91432/1‑150 (MQ=255)
                                       tCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGCTGAAGGCCAGCGCCTCCAGATCGTAATCa                                                                                                        >  2:713991/1‑150 (MQ=255)
                                          gCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCaaa                                                                                                      >  1:587529/1‑149 (MQ=255)
                                          gCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCaaa                                                                                                      <  2:65885/149‑1 (MQ=255)
                                             ttGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAacac                                                                                                   <  2:579141/149‑1 (MQ=255)
                                                aCTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGAc                                                                                              >  2:1139855/1‑151 (MQ=255)
                                                    cttcGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCttt                                                                                          >  1:388434/1‑151 (MQ=255)
                                                      tcGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCttttt                                                                                        >  1:207747/1‑151 (MQ=255)
                                                      tcGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCttt                                                                                          <  1:717238/149‑1 (MQ=255)
                                                             aGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGc                                                                                 >  2:1017105/1‑151 (MQ=255)
                                                              ggCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGcc                                                                                <  1:861149/151‑1 (MQ=255)
                                                               gCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGcc                                                                                >  1:363028/1‑150 (MQ=255)
                                                               gCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCa                                                                               <  1:1161477/151‑1 (MQ=255)
                                                                 cAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGc                                                                             >  1:34489/1‑151 (MQ=255)
                                                                  aGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCt                                                                            <  1:6959/151‑1 (MQ=255)
                                                                   gTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCtt                                                                           <  2:887364/151‑1 (MQ=255)
                                                                    tttGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTc                                                                          >  2:486750/1‑151 (MQ=255)
                                                                      tGACGGCGGCGGTGGAGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTc                                                                          >  1:1010660/1‑149 (MQ=255)
                                                                       gACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAg                                                                        <  2:137236/150‑1 (MQ=255)
                                                                          ggcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGaaa                                                                    <  1:1261466/151‑1 (MQ=255)
                                                                          ggcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGaaa                                                                    <  2:33418/151‑1 (MQ=255)
                                                                          ggcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGaa                                                                     >  2:1257691/1‑150 (MQ=255)
                                                                           gcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAg                                                                   <  1:1189896/151‑1 (MQ=255)
                                                                            cggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGaaa                                                                    >  1:944408/1‑149 (MQ=255)
                                                                             ggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGc                                                                  >  2:1099939/1‑150 (MQ=255)
                                                                               cggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATc                                                               <  1:130290/151‑1 (MQ=255)
                                                                                   ggCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTAc                                                           >  2:597676/1‑151 (MQ=255)
                                                                                   ggCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTAc                                                           <  1:1140833/151‑1 (MQ=255)
                                                                                    gCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTAc                                                           >  2:1125052/1‑150 (MQ=255)
                                                                                     cGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACaa                                                         <  2:1091376/151‑1 (MQ=255)
                                                                                      gATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACaa                                                         >  2:1030137/1‑150 (MQ=255)
                                                                                       atatCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAAt                                                       <  1:430839/151‑1 (MQ=255)
                                                                                            gTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGTCCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCt                                                  <  1:331086/151‑1 (MQ=255)
                                                                                            gTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTCGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCt                                                  <  2:69290/151‑1 (MQ=255)
                                                                                                gagCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAAt                                              <  2:976149/151‑1 (MQ=255)
                                                                                                gagCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAAt                                              <  1:845594/151‑1 (MQ=255)
                                                                                                   ccAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCAGCTCCTCTTGCTGCTTACCTATGAACGCCAGCCCCTCCAGACCGTAATCAAACACCTGACCTTTTTTGTCCGCCATCTTCAGTAAAGCATCTTACAAATTGTCTTAATtat                                           <  1:345928/151‑1 (MQ=255)
                                                                                                    cAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATtata                                          <  1:424642/151‑1 (MQ=255)
                                                                                                     aGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTatat                                         <  2:342448/151‑1 (MQ=255)
                                                                                                       aCTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTc                                       <  2:383878/151‑1 (MQ=255)
                                                                                                       aCTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTc                                       <  2:1196782/151‑1 (MQ=255)
                                                                                                        cTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTc                                       >  2:932723/1‑150 (MQ=255)
                                                                                                          gCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCa                                      <  1:519717/149‑1 (MQ=255)
                                                                                                          gCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCAct                                    >  2:558997/1‑151 (MQ=255)
                                                                                                          gCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCAct                                    >  1:765699/1‑151 (MQ=255)
                                                                                                            aCGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActtt                                  <  1:702531/151‑1 (MQ=255)
                                                                                                            aCGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActtt                                  >  1:68574/1‑151 (MQ=255)
                                                                                                            aCGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActtt                                  >  2:443193/1‑151 (MQ=255)
                                                                                                               cTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttctt                               >  2:242394/1‑151 (MQ=255)
                                                                                                               cTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttctt                               >  1:577041/1‑151 (MQ=255)
                                                                                                                 gAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTta                             <  2:1107427/151‑1 (MQ=255)
                                                                                                                 gAAGTAATCCAGACGGAAATGCTCCGGCTACTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACTTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCGcttgct                                <  2:913281/148‑1 (MQ=255)
                                                                                                                  aaGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTtat                            <  2:233323/151‑1 (MQ=255)
                                                                                                                  aaGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTtat                            <  2:47641/151‑1 (MQ=255)
                                                                                                                   aGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTtata                           <  2:1065182/151‑1 (MQ=255)
                                                                                                                         ccAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCa                      <  1:166985/150‑1 (MQ=255)
                                                                                                                         ccAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCa                      <  1:1139791/150‑1 (MQ=255)
                                                                                                                              cGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCa                 >  1:1033602/1‑150 (MQ=255)
                                                                                                                               ggAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAATGCCAGCGCCTCCAGATCGTAATCAAACACCTGACATTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATc               >  1:720501/1‑151 (MQ=255)
                                                                                                                               ggAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATc               <  1:800141/151‑1 (MQ=255)
                                                                                                                                 aaaTGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATcc              >  1:1024310/1‑150 (MQ=255)
                                                                                                                                  aaTGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCAt            <  2:1404/151‑1 (MQ=255)
                                                                                                                                  aaTGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCAt            <  2:1095679/151‑1 (MQ=255)
                                                                                                                                   aTGCTCCGGCTCCTCTTGCTGCTTACCGATTAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATg           <  2:711357/151‑1 (MQ=255)
                                                                                                                                    tGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGc          >  1:996134/1‑151 (MQ=255)
                                                                                                                                    tGCACCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCATACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGc          >  1:354362/1‑151 (MQ=255)
                                                                                                                                       tCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGAt       >  2:156225/1‑151 (MQ=255)
                                                                                                                                        ccGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGAt       <  2:247662/150‑1 (MQ=255)
                                                                                                                                            ctcctcTTGCTGCTTACCGATGCAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTc  >  2:1055021/1‑151 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTTC  >  NC_000913/82229‑82519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: