Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,704,875 G→A intergenic (+199/‑74) blr → / → cnu beta‑lactam resistance membrane protein; divisome‑associated protein/nucleoid‑associated oriC‑binding protein; H‑NS and StpA stabilizing factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,704,8750GA99.0% 375.6 / NA 101intergenic (+199/‑74)blr/cnubeta‑lactam resistance membrane protein; divisome‑associated protein/nucleoid‑associated oriC‑binding protein; H‑NS and StpA stabilizing factor
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (55/45);  total (55/46)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCGTTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACC  >  NC_000913/1704727‑1705021
                                                                                                                                                    |                                                                                                                                                  
ttGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCAtt                                                                                                                                                  >  2:898849/1‑151 (MQ=255)
  gTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTc                                                                                                                                                 >  2:155385/1‑150 (MQ=255)
  gTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCa                                                                                                                                                <  1:767315/151‑1 (MQ=255)
     ggATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACtt                                                                                                                                             >  1:23956/1‑151 (MQ=255)
     ggATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACtt                                                                                                                                             >  2:321272/1‑151 (MQ=255)
           tAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTg                                                                                                                                       >  1:1098297/1‑151 (MQ=255)
            aaaCCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTg                                                                                                                                       >  1:90774/1‑150 (MQ=255)
               ccAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAAt                                                                                                                                   >  1:1141487/1‑151 (MQ=255)
               ccAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAAt                                                                                                                                   <  2:235920/151‑1 (MQ=255)
                cAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAAtt                                                                                                                                  >  1:975700/1‑151 (MQ=255)
                  ggAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAAtttt                                                                                                                                <  1:1082039/151‑1 (MQ=255)
                   gAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTa                                                                                                                               <  2:1098732/151‑1 (MQ=255)
                    aaTTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTat                                                                                                                              <  2:1147736/151‑1 (MQ=255)
                     aTTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTatc                                                                                                                             <  2:483624/151‑1 (MQ=255)
                     aTTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTatc                                                                                                                             >  1:53960/1‑151 (MQ=255)
                       ttattaTCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTatcat                                                                                                                           >  1:537635/1‑151 (MQ=255)
                             tCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACta                                                                                                                      >  2:401678/1‑150 (MQ=255)
                                tGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACtaata                                                                                                                   <  2:1120290/150‑1 (MQ=255)
                                tGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACtaat                                                                                                                    <  1:349835/149‑1 (MQ=255)
                                 gTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTaataat                                                                                                                 >  2:622662/1‑151 (MQ=255)
                                   tCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCTCTTTTGGTGAAATTTTATCATCACTAataata                                                                                                                <  1:956291/150‑1 (MQ=255)
                                    cGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAAtacc                                                                                                              <  1:642697/151‑1 (MQ=255)
                                     gATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAAtacct                                                                                                             <  1:1213814/151‑1 (MQ=255)
                                     gATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAAtacct                                                                                                             <  1:815633/151‑1 (MQ=255)
                                      aTGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAAtaccta                                                                                                            <  2:594617/151‑1 (MQ=255)
                                      aTGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAAtaccta                                                                                                            <  1:1198859/151‑1 (MQ=255)
                                        gttgttGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAAtacctacc                                                                                                          >  2:211075/1‑151 (MQ=255)
                                         ttgttgGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTAccc                                                                                                         >  1:1110955/1‑151 (MQ=255)
                                            ttgGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCtt                                                                                                      >  1:883243/1‑151 (MQ=255)
                                               gTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTgg                                                                                                   >  2:1008918/1‑151 (MQ=255)
                                               gTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTATTATTATTGTAATAACACAAGCGTTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTCCCCCtt                                                                                                      <  1:1019288/148‑1 (MQ=255)
                                                 gATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGt                                                                                                  >  2:1084671/1‑150 (MQ=255)
                                                  aTTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTTCCCCTTTGGt                                                                                                  <  1:82210/149‑1 (MQ=255)
                                                  aTTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTa                                                                                                 <  1:510643/150‑1 (MQ=255)
                                                        aGGGATAGTAAATCGCGTTGCTCTTTTCAGAGCACTTTTTCAGCCCTGAATATTTGCTATCAGCTTATTCTTATTATTGTAATAACACAACCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTAACCCTTTGGTattaatta                                                                                          <  1:1096427/151‑1 (MQ=255)
                                                          ggATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAattatt                                                                                        <  1:368268/151‑1 (MQ=255)
                                                          ggATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAattatt                                                                                        <  1:398280/151‑1 (MQ=255)
                                                           gATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTAttt                                                                                       >  2:830620/1‑151 (MQ=255)
                                                             tAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTAtttt                                                                                      >  2:1081316/1‑150 (MQ=255)
                                                              aGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTACTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTAt                                                                                    >  2:396497/1‑151 (MQ=255)
                                                                 aaaGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGa                                                                                 <  2:372556/151‑1 (MQ=255)
                                                                  aaGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGAc                                                                                >  2:201545/1‑151 (MQ=255)
                                                                  aaGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGAc                                                                                >  2:394797/1‑151 (MQ=255)
                                                                        gTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTa                                                                          <  1:308122/151‑1 (MQ=255)
                                                                         ttACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTAt                                                                         >  1:748696/1‑151 (MQ=255)
                                                                         ttACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTAt                                                                         >  2:1253198/1‑151 (MQ=255)
                                                                          tACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTAt                                                                         <  1:216877/150‑1 (MQ=255)
                                                                           aCTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATg                                                                        <  2:261946/150‑1 (MQ=255)
                                                                           aCTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATg                                                                        <  2:785206/150‑1 (MQ=255)
                                                                            ctctTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGAc                                                                      >  1:809900/1‑151 (MQ=255)
                                                                                tttCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACt                                                                     <  2:962024/148‑1 (MQ=255)
                                                                                tttCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGt                                                                   <  2:598729/150‑1 (MQ=255)
                                                                                tttCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGt                                                                   <  2:112769/150‑1 (MQ=255)
                                                                                tttCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGt                                                                   <  1:489595/150‑1 (MQ=255)
                                                                                 ttCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGtt                                                                  >  2:689370/1‑150 (MQ=255)
                                                                                  tCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTc                                                                 >  2:879661/1‑150 (MQ=255)
                                                                                  tCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTc                                                                 >  2:739820/1‑150 (MQ=255)
                                                                                    agagCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAgg                                                              >  1:1138803/1‑151 (MQ=255)
                                                                                        cAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTCTATGGACCTTTTATGACTGTTCAGGacta                                                          <  1:432596/151‑1 (MQ=255)
                                                                                          gTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGactact                                                        >  1:73191/1‑151 (MQ=255)
                                                                                            ttttCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACtt                                                       >  1:746696/1‑150 (MQ=255)
                                                                                               tCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACttatt                                                    >  1:705800/1‑150 (MQ=255)
                                                                                                cATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACttatta                                                   >  2:1140146/1‑150 (MQ=255)
                                                                                                cATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACttatta                                                   >  2:630712/1‑150 (MQ=255)
                                                                                                   cccTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTaaaa                                                <  2:453821/150‑1 (MQ=255)
                                                                                                    ccTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAAtt                                              <  1:50242/151‑1 (MQ=255)
                                                                                                      tGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTc                                            >  1:3234/1‑151 (MQ=255)
                                                                                                         atatTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCa                                         <  1:447191/151‑1 (MQ=255)
                                                                                                         atatTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCa                                         <  2:1068943/151‑1 (MQ=255)
                                                                                                           atTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCaa                                        <  1:210003/150‑1 (MQ=255)
                                                                                                            tttGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCaaa                                       >  1:1021129/1‑150 (MQ=255)
                                                                                                             ttGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCaaaa                                      <  1:446981/150‑1 (MQ=255)
                                                                                                             ttGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCaaaa                                      <  2:257855/150‑1 (MQ=255)
                                                                                                              tGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAAt                                     <  1:1069005/150‑1 (MQ=255)
                                                                                                              tGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATc                                    >  1:974774/1‑151 (MQ=255)
                                                                                                                  aTCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGtt                                >  2:1111310/1‑151 (MQ=255)
                                                                                                                  aTCAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGtt                                >  2:351931/1‑151 (MQ=255)
                                                                                                                    cAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCa                              <  2:744851/151‑1 (MQ=255)
                                                                                                                    cAGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCa                              <  1:771709/151‑1 (MQ=255)
                                                                                                                     aGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCAc                             >  1:173646/1‑151 (MQ=255)
                                                                                                                     aGCGCATTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCAc                             >  2:932269/1‑151 (MQ=255)
                                                                                                                          aTTCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGa                         >  1:1015690/1‑150 (MQ=255)
                                                                                                                           ttCTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGaa                        >  1:816744/1‑150 (MQ=255)
                                                                                                                             cTTATTATTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGt                     >  1:247355/1‑151 (MQ=255)
                                                                                                                              ttattaTTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTc                    >  1:350225/1‑151 (MQ=255)
                                                                                                                               tattatTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAg                      <  2:160629/148‑1 (MQ=255)
                                                                                                                               tattatTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTc                    <  2:778548/150‑1 (MQ=255)
                                                                                                                               tattatTGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCt                   >  1:785016/1‑151 (MQ=255)
                                                                                                                                attattGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCt                   <  2:843203/150‑1 (MQ=255)
                                                                                                                                   attGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGa                <  1:898804/150‑1 (MQ=255)
                                                                                                                                   attGTAATAACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGa                >  2:1251921/1‑150 (MQ=255)
                                                                                                                                    ctGTAATCACACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGaa               >  1:841492/2‑150 (MQ=255)
                                                                                                                                      tttaaaaCACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCGTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGaaaa             <  2:555007/145‑1 (MQ=255)
                                                                                                                                       taataaCACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAAc           >  1:813819/1‑151 (MQ=255)
                                                                                                                                          taaCACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAActct        >  2:479514/1‑151 (MQ=255)
                                                                                                                                          taaCACAAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAActct        >  2:240172/1‑151 (MQ=255)
                                                                                                                                              acaAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTAc      >  2:953921/1‑149 (MQ=255)
                                                                                                                                              acaAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACg     >  1:299491/1‑150 (MQ=255)
                                                                                                                                               caAGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGAc   <  1:89683/151‑1 (MQ=255)
                                                                                                                                                aaGCATTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGAcc  >  2:406618/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTGTCGGATATTAAACCAGGAATTTATTATCTTGTTCGATGTTGTTGGTGATTGTCAGGGATAGTAAAGCGCGTTACTCTTTTCAGAGCAGTTTTTCATCCCTGAATATTTGCCATCAGCGCATTCTTATTATTGTAATAACACAAGCGTTCACTTTTGGTGAAATTTTATCATCACTAATAATACCTACCCCTTTGGTATTAATTATTTTATGGACCTTTTATGACTGTTCAGGACTACTTATTAAAATTTCGCAAAATCAGTTCACTCGAAAGTCTGGAAAAACTCTACGACC  >  NC_000913/1704727‑1705021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: