Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,181,988	G→A	W298* (TGG→TAG)	yegV →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,181,988	0	G	A	97.6%	280.6 / ‑4.2	83	W298* (TGG→TAG)	yegV	putative kinase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (38/43);  total (40/43)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACC  >  NC_000913/2181842‑2182134                                                                                                                                                    |                                                                                                                                                   cgCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGtt                                                                                                                                                <  1:451413/151‑1 (MQ=255)  gCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGtt                                                                                                                                                >  2:721602/1‑150 (MQ=255)        ggCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGc                                                                                                                                         <  1:931052/151‑1 (MQ=255)              aTTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCCTGCCGGTGGCGTACTTGCCGGGCTGGGCTCTGGTCTGCCACTGGCGGTTGGGCGCCTTCTTGGGC‑ATGCCGTGGCGGCGTGGGGTGTTGGGCATggg                                                                                                                                    >  1:776040/1‑150 (MQ=255)              aTTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCggg                                                                                                                                   <  2:570992/151‑1 (MQ=255)               ttCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCgggg                                                                                                                                  >  2:400827/1‑151 (MQ=255)                   ggCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCgg                                                                                                                               >  2:1179737/1‑150 (MQ=255)                      aTTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTTTCGGGCATCGGGGCgggg                                                                                                                             >  2:812260/1‑147 (MQ=255)                              cGCAAGTTGTTGACACCATTGGGGCGGGCGACCGTCATGCCGGGGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGGATTACTGGGCAATTCCATGGCGTCGTAGGTTGTCGGGCATCCGGGGGGTGATTGGGGGcc                                                                                                                   >  1:456146/1‑151 (MQ=255)                                cAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGGAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGAATGTGCGcc                                                                                                                   >  2:916206/1‑149 (MQ=255)                                 aaGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTGTTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATTGGGGGGGTGAATGGGCGcc                                                                                                                   >  2:195712/1‑148 (MQ=255)                                 aaGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAAc                                                                                                                <  2:834365/151‑1 (MQ=255)                                   gTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAAcgc                                                                                                              <  1:652862/151‑1 (MQ=255)                                   gTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAAcg                                                                                                               <  2:1064325/150‑1 (MQ=255)                                   gTTGGCGACGCCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAAcgc                                                                                                              <  1:1117823/151‑1 (MQ=255)                                     tGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGGATCGGGGCGGTGATTGTGCGCCAAcg                                                                                                               >  2:98922/1‑148 (MQ=255)                                      gTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCAAagcgcg                                                                                                           >  2:94223/1‑151 (MQ=255)                                        cGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGGCCTAGGGTGTCGGGCATCGGGGGGGGGATTGTGCGCCAAcacgcgcg                                                                                                         >  1:435240/1‑149 (MQ=255)                                          acacCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGGAATGCAGTGGCGTCGTAGGGTGTCGGGGATCGGGGCGGGGGTTGTGGGCCAACCCGCgggg                                                                                                        >  1:1247877/1‑150 (MQ=255)                                          acacCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAgg                                                                                                        <  1:811674/150‑1 (MQ=255)                                          acacCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGa                                                                                                       <  1:749984/151‑1 (MQ=255)                                           cacCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGaa                                                                                                      <  1:512015/151‑1 (MQ=255)                                           cacCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTTTGCGCCAACGCGCGAGGa                                                                                                       >  2:189362/1‑150 (MQ=255)                                                       gggcgACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGCATTACTGGGCAATGCAGTGGCGTCGTAGGGTGTCGGGCATCGGGGCGGGGGTTTGGCGGCAACGCGCGAGGGAATAATCCCCGc                                                                                           >  1:596483/1‑150 (MQ=255)                                                          cgACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGcacac                                                                                       >  2:81533/1‑151 (MQ=255)                                                          cgACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGCATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCCAATCGCGGGGAACAACATCTCGcacac                                                                                       >  2:581990/1‑151 (MQ=255)                                                            aCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGcacaca                                                                                      <  1:989463/150‑1 (MQ=255)                                                              aGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACaaa                                                                                    >  2:1097437/1‑150 (MQ=255)                                                              aGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGGGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCAACCCGCGAGGAACTACTCCTCGGACACaaa                                                                                    >  2:116133/1‑150 (MQ=255)                                                              aGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGGATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCAACGCGCGAGGAACTACCCCTCGCACACaaa                                                                                    >  1:778172/1‑150 (MQ=255)                                                               gTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCGGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACaaaa                                                                                   <  1:964823/150‑1 (MQ=255)                                                               gTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACaaaaa                                                                                  <  1:570955/151‑1 (MQ=255)                                                                 cATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGGCTGCCACTGGCGGAT‑GCCGTAATACTGGGCAATGGAGGGGGGTCGGACTCTGTCGGGCATCGGGGCGGAGATTTGGCGCCCACGCGCGCGGAACGTCTGCTCGccaaccaaa                                                                                   >  2:14665/1‑140 (MQ=255)                                                                     ccGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGtata                                                                            <  1:247937/151‑1 (MQ=255)                                                                     ccGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGATGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGtata                                                                            <  1:338020/151‑1 (MQ=255)                                                                      cGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAg                                                                           <  2:207691/151‑1 (MQ=255)                                                                           gCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTTGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGc                                                                      <  2:1001956/151‑1 (MQ=255)                                                                            cGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCt                                                                     <  2:40484/151‑1 (MQ=255)                                                                            cGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCt                                                                     <  1:799049/151‑1 (MQ=255)                                                                             gTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACCGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCAACCCGCGAGGAAATACTCCTCGCACACAAAAAAGTATAGAt                                                                         >  1:412564/1‑146 (MQ=255)                                                                               aCTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCg                                                                  <  1:94781/151‑1 (MQ=255)                                                                               aCTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACCCGCGAGGAACTACTCCTCCCACACAAAAACGTATAGATCGCTGCg                                                                  >  2:1082179/1‑151 (MQ=255)                                                                                  tGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGa                                                                 <  1:88316/149‑1 (MQ=255)                                                                                   gCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                               <  1:914937/150‑1 (MQ=255)                                                                                      gggCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTTCTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTgg                                                           >  2:302890/1‑151 (MQ=255)                                                                                      gggCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGGATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCCCGCACACAAAAACGGATAGATCGCTGCGACAGTg                                                            >  2:341657/1‑150 (MQ=255)                                                                                        gCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCt                                                         <  2:545434/151‑1 (MQ=255)                                                                                          tGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTTTGCGCCAAAGCGCGGGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCt                                                         >  2:442880/1‑149 (MQ=255)                                                                                                ctGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCt                                                   <  2:179688/149‑1 (MQ=255)                                                                                                  ggTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTg                                                  <  1:175781/148‑1 (MQ=255)                                                                                                  ggTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTa                                                <  1:466532/150‑1 (MQ=255)                                                                                                  ggTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTa                                                <  1:319843/150‑1 (MQ=255)                                                                                                   gTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTAc                                               >  2:874368/1‑150 (MQ=255)                                                                                                    tCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGGCAACGCGCGAGGAACTAATCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTAc                                               >  2:525189/1‑149 (MQ=255)                                                                                                       gCCACTGGCGGAT‑GCCGTATTTCTGGGCAATGCAGTGGCGTCGCAGGTTGTCGGGCATCGGGGCGGGGGATGTGGGCCCACCCGCGAGGAAATAATCCTCGCACACAAAAAAGGAAAGATCGCTGCGGCAGGGGCTAATTCt                                                   >  1:882522/1‑142 (MQ=255)                                                                                                       gCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAAt                                          <  1:718048/151‑1 (MQ=255)                                                                                                       gCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAAt                                          <  2:474547/151‑1 (MQ=255)                                                                                                          aCTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAgg                                       <  2:326375/151‑1 (MQ=255)                                                                                                             ggCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGc                                      <  1:40839/149‑1 (MQ=255)                                                                                                               cGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGtt                                  <  2:791072/151‑1 (MQ=255)                                                                                                                ggAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTg                                 <  1:650356/151‑1 (MQ=255)                                                                                                                 gAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGc                                >  2:293165/1‑151 (MQ=255)                                                                                                                     gCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGt                             >  2:103587/1‑151 (MQ=255)                                                                                                                      ccGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGtt                            <  2:97331/151‑1 (MQ=255)                                                                                                                       cGTATTACTGGGGAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTg                              <  2:781403/148‑1 (MQ=255)                                                                                                                         tATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTgg                          >  2:1180594/1‑150 (MQ=255)                                                                                                                          aTTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                        <  2:577687/151‑1 (MQ=255)                                                                                                                           ttACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGt                         >  1:473060/1‑149 (MQ=255)                                                                                                                            tACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                       <  1:203686/150‑1 (MQ=255)                                                                                                                            tACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                       >  2:90590/1‑150 (MQ=255)                                                                                                                                  gCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGcc                 >  1:139319/1‑150 (MQ=255)                                                                                                                                    aaTGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCAct              >  1:803705/1‑151 (MQ=255)                                                                                                                                     aTGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActt             <  2:874857/151‑1 (MQ=255)                                                                                                                                        cAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgct          >  1:55156/1‑151 (MQ=255)                                                                                                                                          gTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg        <  1:1030419/151‑1 (MQ=255)                                                                                                                                          gTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgctt         <  1:452040/150‑1 (MQ=255)                                                                                                                                             gCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATc     <  1:813241/151‑1 (MQ=255)                                                                                                                                              cgtcgtAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCa    <  2:1243749/151‑1 (MQ=255)                                                                                                                                               gtcgtAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAc   >  1:582461/1‑151 (MQ=255)                                                                                                                                               gtcgtAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAc   >  2:463591/1‑151 (MQ=255)                                                                                                                                               gtcgtAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAc   >  1:532417/1‑151 (MQ=255)                                                                                                                                                tcgtAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGGGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTTGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgctt         <  1:56996/144‑1 (MQ=255)                                                                                                                                                tcgtAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGGCAAGCGCCACTTGCTTGATCAcc  >  2:117258/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAT‑GCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACC  >  NC_000913/2181842‑2182134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑