Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,493,798	A→G	I202T (ATC→ACC)	yfdX ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,493,798	0	A	G	97.3%	271.0 / ‑3.6	73	I202T (ATC→ACC)	yfdX	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (32/39);  total (33/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGG  >  NC_000913/2493651‑2493944                                                                                                                                                    |                                                                                                                                                   ttATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACggtg                                                                                                                                                  >  2:977932/1‑150 (MQ=255)     aGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTcc                                                                                                                                             >  2:169280/1‑151 (MQ=255)        aCCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATc                                                                                                                                          <  1:378492/151‑1 (MQ=255)          cgcgAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCtt                                                                                                                                        >  1:901730/1‑151 (MQ=255)                   cAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCtt                                                                                                                               <  2:997982/151‑1 (MQ=255)                             tATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCa                                                                                                                      <  2:395591/150‑1 (MQ=255)                               tGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGt                                                                                                                   >  1:311604/1‑151 (MQ=255)                                 aCCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTTAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGttt                                                                                                                 <  2:855145/151‑1 (MQ=255)                                 aCCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGttt                                                                                                                 >  2:175271/1‑151 (MQ=255)                                     caACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCt                                                                                                             <  2:372714/151‑1 (MQ=255)                                           gTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCgtag                                                                                                        <  2:395559/150‑1 (MQ=255)                                             cGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTAt                                                                                                     <  1:669642/151‑1 (MQ=255)                                                aTTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGc                                                                                                  <  1:635866/151‑1 (MQ=255)                                                   gtgGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCtt                                                                                                <  1:94065/150‑1 (MQ=255)                                                    tgGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCtttt                                                                                              >  2:310478/1‑151 (MQ=255)                                                     ggTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCtttt                                                                                              <  2:676561/150‑1 (MQ=255)                                                     ggTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCtttt                                                                                              <  1:513814/150‑1 (MQ=255)                                                      gTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTa                                                                                            <  1:317361/151‑1 (MQ=255)                                                      gTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTa                                                                                            <  1:518932/151‑1 (MQ=255)                                                        cATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATc                                                                                          >  1:832461/1‑151 (MQ=255)                                                           aaaaaaTTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCa                                                                                         >  2:792672/1‑149 (MQ=255)                                                            aaaaaTTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGc                                                                                      >  1:756502/1‑151 (MQ=255)                                                               aaTTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGt                                                                                   <  1:369640/151‑1 (MQ=255)                                                                  tGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTc                                                                                >  2:641404/1‑151 (MQ=255)                                                                  tGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTc                                                                                >  2:908673/1‑151 (MQ=255)                                                                   gTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCt                                                                               <  1:106117/151‑1 (MQ=255)                                                                   gTCGGCTGGAGGGAGTTAAATCCTTTACCGCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTc                                                                                <  2:725135/150‑1 (MQ=255)                                                                     cGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTgc                                                                             <  2:322513/151‑1 (MQ=255)                                                                      ggCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTgcg                                                                            <  2:666370/151‑1 (MQ=255)                                                                         tGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCa                                                                          >  1:1260987/1‑150 (MQ=255)                                                                           gagggagTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCa                                                                       <  1:1046420/151‑1 (MQ=255)                                                                              ggagTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCGCCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCa                                                                    <  2:142777/151‑1 (MQ=255)                                                                                agTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCa                                                                    >  1:1136530/1‑149 (MQ=255)                                                                                     aaTCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGAATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGAGCa                                                              >  2:136940/1‑150 (MQ=255)                                                                                      aTCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCAtt                                                            <  1:1177932/151‑1 (MQ=255)                                                                                        ccTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTa                                                           <  1:1247155/150‑1 (MQ=255)                                                                                        ccTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTAc                                                          >  2:902800/1‑151 (MQ=255)                                                                                          tttACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACg                                                         >  1:572403/1‑150 (MQ=255)                                                                                             aCCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGt                                                      <  2:188877/150‑1 (MQ=255)                                                                                               cTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTg                                                   >  1:1251081/1‑151 (MQ=255)                                                                                                  aaTATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGttt                                                <  2:171601/151‑1 (MQ=255)                                                                                                  aaTATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGttt                                                >  2:1083108/1‑151 (MQ=255)                                                                                                   atatACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGttt                                                >  2:1167512/1‑150 (MQ=255)                                                                                                   atatACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGttt                                                >  1:148116/1‑150 (MQ=255)                                                                                                     ataCTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTaa                                              <  2:403367/150‑1 (MQ=255)                                                                                                     ataCTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTa                                               >  2:701803/1‑149 (MQ=255)                                                                                                     ataCTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAg                                             <  1:709653/151‑1 (MQ=255)                                                                                                     ataCTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCACACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTa                                               >  1:55749/1‑149 (MQ=255)                                                                                                      taCTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAg                                             >  1:1080968/1‑150 (MQ=255)                                                                                                       aCTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGt                                            <  2:651797/150‑1 (MQ=255)                                                                                                       aCTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTg                                           <  2:838137/151‑1 (MQ=255)                                                                                                        cTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTgg                                          >  1:1029533/1‑151 (MQ=255)                                                                                                          gCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCa                                        <  2:477/151‑1 (MQ=255)                                                                                                              gTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATc                                      >  1:470142/1‑149 (MQ=255)                                                                                                               tCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTa                                               <  2:400099/139‑1 (MQ=255)                                                                                                               tCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTa                                               >  1:400090/1‑139 (MQ=255)                                                                                                                  gCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCTTCAGatat                                >  1:867414/1‑151 (MQ=255)                                                                                                                  gCATTAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGatat                                <  2:542910/151‑1 (MQ=255)                                                                                                                      tAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGt                            >  1:899811/1‑151 (MQ=255)                                                                                                                      tAGTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGt                            >  1:1259124/1‑151 (MQ=255)                                                                                                                        gTTCACAAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGttt                          <  1:376599/151‑1 (MQ=255)                                                                                                                           cacaAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCATCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTc                        >  2:1058515/1‑150 (MQ=255)                                                                                                                            acaAACAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCa                      <  2:1167903/151‑1 (MQ=255)                                                                                                                               aaCAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCAtt                    >  2:1038983/1‑150 (MQ=255)                                                                                                                               aaCAGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTa                   <  2:1085858/151‑1 (MQ=255)                                                                                                                                aCAGTGCTTCGTTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTAc                  >  2:563789/1‑151 (MQ=255)                                                                                                                                  aGTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGc                <  1:941904/151‑1 (MQ=255)                                                                                                                                   gTGCTTCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGcc               <  1:1039033/151‑1 (MQ=255)                                                                                                                                        tCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAacac          >  1:584981/1‑151 (MQ=255)                                                                                                                                        tCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAac            <  2:788938/149‑1 (MQ=255)                                                                                                                                        tCGCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAac            <  1:1139574/149‑1 (MQ=255)                                                                                                                                          gCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCg        <  1:300466/151‑1 (MQ=255)                                                                                                                                          gCTATCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCg        >  1:1030827/1‑151 (MQ=255)                                                                                                                                             aTCGACGGTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGcc     <  2:326166/151‑1 (MQ=255)                                                                                                                                               cgacggTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGcc     >  2:653036/1‑149 (MQ=255)                                                                                                                                                gacggTGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAgg  >  2:313904/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TTATAGCACCGCGAGCGCCAAATAATTTTATGACCACAACCTGTCGTATTGTGGTCATAAAAAATTGTCGGCTGGAGGGAGTTAAATCCTTTACCTCAATATACTGCCTGTCAGCATTAGTTCACAAACAGTGCTTCGCTATCGACGATGATTCCATCTTCCGCACCTTTAAGCGCCAGGTTTGCCTCGTAGTATTGCTTTTTATCAAGCAGTTTCTGCGCATCAGCAAGTGCATTACGAGTCTGTTTAAGTGGCATCAGATATTGGTTTTCCATTACGCCAACACCGGCCAGG  >  NC_000913/2493651‑2493944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑