Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,843,150 G→A A99A (GCC→GCT hycH ← hydrogenase 3 maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,843,1500GA95.8% 333.4 / 3.7 95A99A (GCC→GCThycHhydrogenase 3 maturation protein
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base A (46/45);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

CCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGC  >  NC_000913/2843004‑2843295
                                                                                                                                                  |                                                                                                                                                 
ccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCtt                                                                                                                                               >  1:786392/1‑151 (MQ=255)
ccATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCtt                                                                                                                                               >  2:1208073/1‑151 (MQ=255)
 catcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCttt                                                                                                                                              >  1:275315/1‑151 (MQ=255)
 catcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCttt                                                                                                                                              <  1:1154578/151‑1 (MQ=255)
  atcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCttt                                                                                                                                              >  2:526976/1‑150 (MQ=255)
   tcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTAt                                                                                                                                            >  1:1261161/1‑151 (MQ=255)
    catGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTAt                                                                                                                                            <  2:47392/150‑1 (MQ=255)
     atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCg                                                                                                                                          <  1:532935/151‑1 (MQ=255)
      tGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCg                                                                                                                                          >  1:1190965/1‑150 (MQ=255)
      tGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCg                                                                                                                                          >  2:117845/1‑150 (MQ=255)
       gCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGa                                                                                                                                         >  1:67598/1‑150 (MQ=255)
        cTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAAt                                                                                                                                       >  1:331765/1‑151 (MQ=255)
          aTTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAAt                                                                                                                                       <  1:1020072/149‑1 (MQ=255)
          aTTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGc                                                                                                                                     <  2:603048/151‑1 (MQ=255)
          aTTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGc                                                                                                                                     <  2:145688/151‑1 (MQ=255)
          aTTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGc                                                                                                                                     <  2:652112/151‑1 (MQ=255)
                 acacAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGcc                                                                                                                              <  2:814121/151‑1 (MQ=255)
                 acacAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGcc                                                                                                                              >  1:3793/1‑151 (MQ=255)
                  cacaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCa                                                                                                                             <  1:677217/151‑1 (MQ=255)
                   acaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGcc                                                                                                                              >  1:944703/1‑149 (MQ=255)
                    caGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGc                                                                                                                           >  1:157443/1‑151 (MQ=255)
                      gagTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCAt                                                                                                                         <  2:973591/151‑1 (MQ=255)
                          aaaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGc                                                                                                                     >  1:521091/1‑151 (MQ=255)
                          aaaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGc                                                                                                                     <  2:906665/151‑1 (MQ=255)
                           aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGca                                                                                                                    <  2:487384/151‑1 (MQ=255)
                               gtcagtcaCGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATg                                                                                                                >  2:766540/1‑151 (MQ=255)
                                  agtcaCGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGtt                                                                                                             <  2:64069/151‑1 (MQ=255)
                                               cctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATg                                                                                                   <  2:342447/148‑1 (MQ=255)
                                               cctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGa                                                                                                  <  2:564988/149‑1 (MQ=255)
                                               cctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGAc                                                                                                 <  2:832183/150‑1 (MQ=255)
                                               cctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACg                                                                                                <  1:686554/151‑1 (MQ=255)
                                                ctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAATAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGa                                                                                                  <  1:371811/148‑1 (MQ=255)
                                                  cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGAtc                                                                                             >  2:247047/1‑151 (MQ=255)
                                                  cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGAt                                                                                              <  1:479627/150‑1 (MQ=255)
                                                     cATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCa                                                                                          <  1:568651/151‑1 (MQ=255)
                                                     cATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCa                                                                                          <  1:495967/151‑1 (MQ=255)
                                                      aTCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCAc                                                                                         >  1:1089028/1‑151 (MQ=255)
                                                       tCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCAcc                                                                                        >  1:254560/1‑151 (MQ=255)
                                                          aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCata                                                                                     <  1:1128596/151‑1 (MQ=255)
                                                          aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCat                                                                                      >  1:710232/1‑150 (MQ=255)
                                                           aTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCatat                                                                                    >  2:950556/1‑151 (MQ=255)
                                                            tAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATAtt                                                                                   >  1:127173/1‑151 (MQ=255)
                                                             aGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATAttt                                                                                  >  2:517760/1‑151 (MQ=255)
                                                              gATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTc                                                                                 <  2:625645/151‑1 (MQ=255)
                                                                tGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCgg                                                                               >  1:758867/1‑151 (MQ=255)
                                                                   cGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCAc                                                                            >  2:364121/1‑151 (MQ=255)
                                                                   cGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCAc                                                                            <  1:793333/151‑1 (MQ=255)
                                                                        tCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCt                                                                        <  1:446984/150‑1 (MQ=255)
                                                                        tCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCt                                                                        <  1:664816/150‑1 (MQ=255)
                                                                         ccTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTc                                                                      >  1:13418/1‑151 (MQ=255)
                                                                            gatgaATATCATGCAGCATACTTAACATCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCAt                                                                   <  1:517745/151‑1 (MQ=255)
                                                                               gaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCAtttt                                                                <  2:727251/151‑1 (MQ=255)
                                                                               gaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCAttt                                                                 <  2:448985/150‑1 (MQ=255)
                                                                                aaTATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTg                                                               >  2:870372/1‑151 (MQ=255)
                                                                                 atatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgc                                                              <  1:1090541/151‑1 (MQ=255)
                                                                                 atatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgc                                                              >  1:1083086/1‑151 (MQ=255)
                                                                                 atatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgc                                                              <  1:526657/151‑1 (MQ=255)
                                                                                  tatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcg                                                             >  2:1070317/1‑151 (MQ=255)
                                                                                   atCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCGCCATATTTCGGCACGCCTTCCATTTTgcg                                                             <  2:752385/150‑1 (MQ=255)
                                                                                     cATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcggcg                                                          <  2:374703/151‑1 (MQ=255)
                                                                                       tGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGcggcgg                                                         >  1:107151/1‑150 (MQ=255)
                                                                                        gcagcaTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCggg                                                        >  1:843921/1‑150 (MQ=255)
                                                                                         cagcaTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGcggcgg                                                         >  1:697962/1‑148 (MQ=255)
                                                                                           gcaTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTc                                                    <  1:652740/151‑1 (MQ=255)
                                                                                             aTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCAc                                                  >  1:639204/1‑151 (MQ=255)
                                                                                             aTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCAc                                                  <  1:940938/151‑1 (MQ=255)
                                                                                                  tAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCAccgc                                               >  1:977306/1‑149 (MQ=255)
                                                                                                   aaCAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgcc                                              >  2:208890/1‑149 (MQ=255)
                                                                                                     cAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACtgcctgcc                                          <  1:30393/151‑1 (MQ=255)
                                                                                                      aGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACtgcctgcc                                          >  1:590060/1‑150 (MQ=255)
                                                                                                        cATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTcc                                       >  2:1212970/1‑151 (MQ=255)
                                                                                                        cATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTcc                                       <  1:892305/151‑1 (MQ=255)
                                                                                                          ttGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTcc                                       >  2:100993/1‑149 (MQ=255)
                                                                                                            gTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCCCTGCCTGCCTcccg                                     >  1:948165/1‑147 (MQ=255)
                                                                                                            gTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCaga                                    <  2:801628/150‑1 (MQ=255)
                                                                                                              aCTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGt                                  <  1:534134/150‑1 (MQ=255)
                                                                                                              aCTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGt                                  <  2:890182/150‑1 (MQ=255)
                                                                                                                tCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTCCCTGCCTCCagag                                   >  2:257705/1‑147 (MQ=255)
                                                                                                                 ccATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCa                              <  1:562897/151‑1 (MQ=255)
                                                                                                                   aTTCCTGCTGCTGCGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAAt                            <  2:948218/151‑1 (MQ=255)
                                                                                                                        tgctgctgcGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATg                       >  2:485818/1‑151 (MQ=255)
                                                                                                                         gctgctgcGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATg                       <  2:1004485/150‑1 (MQ=255)
                                                                                                                         gctgctgcGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATg                       <  2:15138/150‑1 (MQ=255)
                                                                                                                         gctgctgcGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGAGGCGGGCTTGACTGCCTGCCTCCAGAGTGGCCATCCACGc                      >  2:462395/1‑151 (MQ=255)
                                                                                                                               gcGAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGata                <  1:1240304/151‑1 (MQ=255)
                                                                                                                                 gAAGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATAtt              <  2:67601/151‑1 (MQ=255)
                                                                                                                                  aaGTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATAtt              >  2:917641/1‑150 (MQ=255)
                                                                                                                                    gTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCAt           <  2:1078229/151‑1 (MQ=255)
                                                                                                                                    gTTTGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTCCCTCCAGAGTGGCAATCCCTGCCAGATATTCa            >  2:888824/1‑150 (MQ=255)
                                                                                                                                     tttGCGCTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGGTTCACTGCCTGCCTCCAGCGGGGCCATCCATGCCAGATAtt              >  1:848914/1‑147 (MQ=255)
                                                                                                                                          gcTGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACg     <  1:802327/151‑1 (MQ=255)
                                                                                                                                            tGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTTACCGCCCGCCTCCAGAGGTGCAATCCCTGCCAGATATTCATccccccgg   >  2:812986/1‑146 (MQ=255)
                                                                                                                                            tGCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCCTCCTCCCGAGTGGCAATCCATGCCAGATATTCATCCCACggg   >  1:205625/1‑151 (MQ=255)
                                                                                                                                             gCCCGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGc  <  2:355943/151‑1 (MQ=255)
                                                                                                                                              cccGAGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGGGGCAATCCATGCCAGATATTCATCCCACggg   >  2:821433/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGC  >  NC_000913/2843004‑2843295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: