Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,617	G→A	L186L (CTG→CTA)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,617	0	G	A	100.0%	259.4 / NA	67	L186L (CTG→CTA)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/31);  total (36/31)

CCTTCTGATTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTGAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCAT  >  NC_000913/3016471‑3016761                                                                                                                                                     |                                                                                                                                                 ccTTCTGATTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAAt                                                                                                                                               >  2:376313/1‑150 (MQ=255)   ttctcgttCCGAAAATTCTTTCATCTTAAATAGGGGCGTGGTTTAC‑AACCTGGAAGGTATA‑AACCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATaa                                                                                                                                             <  1:1187646/144‑1 (MQ=255)      tGATTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAAtt                                                                                                                                          >  2:672218/1‑150 (MQ=255)        aTTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACt                                                                                                                                       >  2:641153/1‑151 (MQ=255)            cGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTcc                                                                                                                                     >  1:81749/1‑149 (MQ=255)               tAAATCGTTCATCTTTAATATGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGAt                                                                                                                                >  1:30757/1‑151 (MQ=255)                    cGTTCATCTTTAATATGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACg                                                                                                                            >  2:702141/1‑150 (MQ=255)                            tttAATATGAGCGTCGGATAC‑AACCTCGAAGGTATT‑AAGCAACCGACGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCCCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAttt                                                                                                                       >  1:134117/1‑147 (MQ=255)                              ttatgaggagcttggTTACAAACCTGAAAGTTATA‑AACCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAttttt                                                                                                                     <  1:853489/135‑1 (MQ=255)                               aatgtgacggTCGTTTAC‑ACCGTCGAAGTTATA‑AGGAAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTc                                                                                                                <  1:706351/142‑1 (MQ=255)                               aaTATGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTc                                                                                                                >  1:671312/1‑151 (MQ=255)                               aaTATGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTc                                                                                                                >  2:1004689/1‑151 (MQ=255)                               aaTAGGGGCGTCGTTCAC‑ACCCTCGAAGGTATA‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGc                                                                                                                  <  2:71927/149‑1 (MQ=255)                                  atGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGcc                                                                                                             >  2:68420/1‑151 (MQ=255)                                    gcgcgctggTAAC‑AACCTAGAATGAATT‑AACCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCg                                                                                                               <  2:19402/140‑1 (MQ=255)                                      gCGTGGTTTAC‑ACCCTAGAAGTTATA‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACgg                                                                                                         <  2:622818/151‑1 (MQ=255)                                        gTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGAt                                                                                                       >  2:201016/1‑151 (MQ=255)                                        gTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGAt                                                                                                       >  2:1007743/1‑151 (MQ=255)                                           ggTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGc                                                                                                    >  1:36155/1‑151 (MQ=255)                                            gttaacacctgagAAGTTATA‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACgg                                                                                                         <  1:723179/134‑1 (MQ=255)                                               aC‑AACCTCGAAGTTATA‑AACCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAgg                                                                                                 <  2:317430/150‑1 (MQ=255)                                                   cccgcGAAGTTATA‑AACCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACg                                                                                                          <  1:623350/134‑1 (MQ=255)                                                   aCCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGaaa                                                                                              >  2:203664/1‑150 (MQ=255)                                                    actgttgggTATA‑AAGCAGCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGAtt                                                                                                      <  1:1128258/134‑1 (MQ=255)                                                     cctggaagTATTAAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGc                                                                                                    <  2:593827/136‑1 (MQ=255)                                                     cTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAc                                                                                           >  1:536748/1‑151 (MQ=255)                                                      aagaaattata‑AAACACCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAc                                                                                           <  1:2591/143‑1 (MQ=255)                                                       cGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAcgc                                                                                         >  2:823308/1‑151 (MQ=255)                                                          aggtttttaacaacccgccgATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAc                                                                                           <  2:1155719/133‑1 (MQ=255)                                                          aGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGa                                                                                       >  1:107411/1‑150 (MQ=255)                                                           ggTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGaa                                                                                      >  2:317686/1‑150 (MQ=255)                                                             tATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGc                                                                                   >  1:116805/1‑151 (MQ=255)                                                               ataaaGCACCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGa                                                                                       <  1:853057/143‑1 (MQ=255)                                                               ataaaGAAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGaa                                                                                      <  1:508089/144‑1 (MQ=255)                                                                  aaGAAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGaaaaa                                                                                            <  2:810967/138‑1 (MQ=255)                                                                       aCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCa                                                                              <  1:220476/147‑1 (MQ=255)                                                                        ccgccgATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCt                                                                         <  1:80903/151‑1 (MQ=255)                                                                          gccgATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTa                                                                       <  2:536763/151‑1 (MQ=255)                                                                            cgATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTAcc                                                                     >  1:890174/1‑151 (MQ=255)                                                                             gATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTAccc                                                                    <  2:504316/151‑1 (MQ=255)                                                                             gATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTAccc                                                                    >  1:825805/1‑151 (MQ=255)                                                                              aTGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCTGGAAAAACGCGAAAGCTTGCAAGCCTTACCCg                                                                   >  2:1247076/1‑151 (MQ=255)                                                                                gCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCt                                                                 <  2:312075/151‑1 (MQ=255)                                                                                    cAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGc                                                              >  1:381056/1‑150 (MQ=255)                                                                                         cATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATcccc                                                        <  2:365259/151‑1 (MQ=255)                                                                                           tCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCccac                                                      >  2:839919/1‑151 (MQ=255)                                                                                                   atgatgGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATgg                                              <  2:378089/151‑1 (MQ=255)                                                                                                   atgatgGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATg                                               <  1:320533/150‑1 (MQ=255)                                                                                                           cGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGc                                      >  2:130224/1‑151 (MQ=255)                                                                                                           cGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCACCCCCACCAGTATGGTGCATGGc                                      >  2:1161255/1‑151 (MQ=255)                                                                                                               tCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTc                                   >  1:949923/1‑150 (MQ=255)                                                                                                                     cATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCctct                            >  2:552971/1‑151 (MQ=255)                                                                                                                     cATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCctct                            >  2:607864/1‑151 (MQ=255)                                                                                                                      aTCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCtctc                           >  2:331643/1‑151 (MQ=255)                                                                                                                        ccGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTcca                         <  1:635390/151‑1 (MQ=255)                                                                                                                        ccGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTcc                          >  1:722306/1‑150 (MQ=255)                                                                                                                         cGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTccac                        <  2:768400/151‑1 (MQ=255)                                                                                                                           aaaTTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTccacc                       >  1:93338/1‑150 (MQ=255)                                                                                                                             aTTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATg                    >  2:713187/1‑151 (MQ=255)                                                                                                                                 gCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACg                >  1:365578/1‑151 (MQ=255)                                                                                                                                    cAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGTATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCt             <  1:76958/151‑1 (MQ=255)                                                                                                                                      atatCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGt           <  2:879276/151‑1 (MQ=255)                                                                                                                                      atatCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGt           <  2:957368/151‑1 (MQ=255)                                                                                                                                            cgATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGtcctcc      <  1:203713/150‑1 (MQ=255)                                                                                                                                            cgATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCg     >  1:149605/1‑151 (MQ=255)                                                                                                                                                aCGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCAt  <  2:519265/150‑1 (MQ=255)                                                                                                                                                 cGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCa   >  1:55225/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CCTTCTGATTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTAC‑AACCTCGAAGGTATT‑AAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTGAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCAT  >  NC_000913/3016471‑3016761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑