Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,057,347 T→C N355S (AAC→AGC)  serA ← D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,3470TC95.2% 224.6 / 0.8 62N355S (AAC→AGC) serAD‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (32/27);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

ATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCC  >  NC_000913/3057200‑3057493
                                                                                                                                                   |                                                                                                                                                  
aTGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCa                                                                                                                                                 <  1:999498/151‑1 (MQ=255)
aTGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCa                                                                                                                                                 <  1:83371/151‑1 (MQ=255)
   gTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAgcg                                                                                                                                              <  2:817181/151‑1 (MQ=255)
     aCCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAgcgc                                                                                                                                             <  2:1189790/150‑1 (MQ=255)
     aCCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAgcgc                                                                                                                                             >  2:115060/1‑150 (MQ=255)
     aCCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAgcgc                                                                                                                                             >  2:1147241/1‑150 (MQ=255)
      cccGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAgcgc                                                                                                                                             >  2:345512/1‑149 (MQ=255)
       ccGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGt                                                                                                                                          <  1:83301/151‑1 (MQ=255)
         ggAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTa                                                                                                                                        <  2:1256870/151‑1 (MQ=255)
               gCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGc                                                                                                                                  >  2:985208/1‑151 (MQ=255)
               gCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGc                                                                                                                                  >  1:299016/1‑151 (MQ=255)
                   tCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCg                                                                                                                               <  1:1127570/150‑1 (MQ=255)
                   tCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCg                                                                                                                               <  2:121993/150‑1 (MQ=255)
                     aTTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGAc                                                                                                                            >  2:562506/1‑151 (MQ=255)
                        gCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGt                                                                                                                         >  2:525164/1‑151 (MQ=255)
                          cTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGttt                                                                                                                       >  2:802756/1‑151 (MQ=255)
                            gCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTc                                                                                                                     >  1:760363/1‑151 (MQ=255)
                               gcgcTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTg                                                                                                                  <  1:1206079/151‑1 (MQ=255)
                                    ttttCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGAtgt                                                                                                             >  1:1139297/1‑151 (MQ=255)
                                     tttCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGAtgt                                                                                                             <  2:596368/150‑1 (MQ=255)
                                      ttCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGAtgtg                                                                                                            <  2:780602/150‑1 (MQ=255)
                                       tCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGc                                                                                                           <  2:770252/150‑1 (MQ=255)
                                        cGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCa                                                                                                          <  1:139097/150‑1 (MQ=255)
                                          gCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCa                                                                                                       <  2:777018/151‑1 (MQ=255)
                                              cGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAgacg                                                                                                   >  2:1192404/1‑151 (MQ=255)
                                                       cgGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCAcc                                                                                          >  1:1132983/1‑151 (MQ=255)
                                                        ggCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCg                                                                                         >  2:1172693/1‑151 (MQ=255)
                                                         gCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGt                                                                                        <  2:820719/151‑1 (MQ=255)
                                                               atatcaataACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTg                                                                                  >  1:838793/1‑151 (MQ=255)
                                                                 atcaataACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGc                                                                                >  1:1195904/1‑151 (MQ=255)
                                                                         ccACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                        <  2:1102006/151‑1 (MQ=255)
                                                                         ccACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                        <  2:794478/151‑1 (MQ=255)
                                                                             aTAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTcc                                                                    >  2:304240/1‑151 (MQ=255)
                                                                               aaCCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCgg                                                                  >  2:1153045/1‑151 (MQ=255)
                                                                               aaCCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCgg                                                                  >  2:669309/1‑151 (MQ=255)
                                                                                 cccATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCggg                                                                 >  2:384484/1‑150 (MQ=255)
                                                                                    aTCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGt                                                             >  2:1153774/1‑151 (MQ=255)
                                                                                       tGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTc                                                           >  1:234902/1‑150 (MQ=255)
                                                                                         ggCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCAcc                                                        <  2:876501/151‑1 (MQ=255)
                                                                                               aGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCag                                                    >  1:484104/1‑149 (MQ=255)
                                                                                                   tGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGc                                               <  2:1093255/150‑1 (MQ=255)
                                                                                                      aGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTg                                           >  2:720009/1‑151 (MQ=255)
                                                                                                         tatTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGc                                        >  2:1149401/1‑151 (MQ=255)
                                                                                                                cggcgATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTc                                 <  1:213147/151‑1 (MQ=255)
                                                                                                                  gcgATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGGCGCCCACCGTGCAGTGGCAGCGCGACTTCCGGGAAGTTCACCGCTGAGAGCGTTGACCCATTGTCAg                               <  1:1192715/151‑1 (MQ=255)
                                                                                                                    gATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGaa                             <  1:1121691/151‑1 (MQ=255)
                                                                                                                    gATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGaa                             <  2:760396/151‑1 (MQ=255)
                                                                                                                    gATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGa                              >  2:399540/1‑150 (MQ=255)
                                                                                                                    gATGTTGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGa                              >  1:371832/1‑150 (MQ=255)
                                                                                                                        ttGACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACt                         >  2:522275/1‑151 (MQ=255)
                                                                                                                          gACGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACtt                        <  2:1221205/150‑1 (MQ=255)
                                                                                                                            cGCCCTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGAt                     <  1:444210/151‑1 (MQ=255)
                                                                                                                              cccTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATc                    >  2:625480/1‑150 (MQ=255)
                                                                                                                               ccTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCaa                  <  1:732916/151‑1 (MQ=255)
                                                                                                                               ccTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCaa                  <  1:640256/151‑1 (MQ=255)
                                                                                                                                cTGCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAAt                 >  2:233547/1‑151 (MQ=255)
                                                                                                                                  gCTCGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAAttt               <  2:933663/151‑1 (MQ=255)
                                                                                                                                     cGGCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAcc            >  1:816219/1‑151 (MQ=255)
                                                                                                                                       gCGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATTCTTGATCAATTTACCCg          <  2:740578/151‑1 (MQ=255)
                                                                                                                                        cGAAGATTTTGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGc         >  2:746605/1‑151 (MQ=255)
                                                                                                                                              ttttGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTc   >  1:169644/1‑151 (MQ=255)
                                                                                                                                               tttGCTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTcc  >  1:672138/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCC  >  NC_000913/3057200‑3057493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑