Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 809,038 C→T A74T (GCC→ACC)  bioA ← 7,8‑diaminopelargonic acid synthase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913809,0380CT94.1% 165.1 / ‑0.5 51A74T (GCC→ACC) bioA7,8‑diaminopelargonic acid synthase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base T (24/24);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

GCCATTTTCATCGCCACTTCCACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGG  >  NC_000913/808890‑809185
                                                                                                                                                    |                                                                                                                                                   
gCCATTTTCATCGCCACTTCCACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTAt                                                                                                                                                   <  2:240458/151‑1 (MQ=255)
  cATTTTCATCGCCACTTCCACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCa                                                                                                                                                 <  2:429385/151‑1 (MQ=255)
       tCATCGCCACTTCCACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAAttt                                                                                                                                             <  2:91364/150‑1 (MQ=255)
        cATCGCCACTTCCACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGc                                                                                                                                           >  2:187825/1‑151 (MQ=255)
           cGCCACTTCCACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCACTTTGCGAc                                                                                                                                        >  1:526410/1‑151 (MQ=255)
                cTTCCACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCAt                                                                                                                                   >  2:637839/1‑151 (MQ=255)
                 ttCCACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATc                                                                                                                                  >  1:516519/1‑151 (MQ=255)
                  tCCACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCAt                                                                                                                                   <  1:30423/149‑1 (MQ=255)
                   ccACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATcg                                                                                                                                 <  2:923505/150‑1 (MQ=255)
                    cACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATcgcc                                                                                                                               <  2:147888/151‑1 (MQ=255)
                    cACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATc                                                                                                                                  <  2:205765/148‑1 (MQ=255)
                     aCCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATcgccg                                                                                                                              >  2:1067575/1‑151 (MQ=255)
                     aCCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATcgccg                                                                                                                              >  2:871565/1‑151 (MQ=255)
                         cTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCAtt                                                                                                                          >  1:411398/1‑151 (MQ=255)
                           aCGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTa                                                                                                                         <  1:623780/150‑1 (MQ=255)
                            cGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTa                                                                                                                         >  1:537471/1‑149 (MQ=255)
                             ggAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGc                                                                                                                      >  2:577930/1‑151 (MQ=255)
                              gAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCt                                                                                                                     >  1:304574/1‑151 (MQ=255)
                                    gAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGt                                                                                                               >  2:550000/1‑151 (MQ=255)
                                      gTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTg                                                                                                              >  2:752986/1‑150 (MQ=255)
                                        ccGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGAtt                                                                                                           <  2:442919/151‑1 (MQ=255)
                                        ccGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGAtt                                                                                                           >  1:777073/1‑151 (MQ=255)
                                           cgAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGt                                                                                                         <  1:1224936/150‑1 (MQ=255)
                                               aaaaaCGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCg                                                                                                    <  2:903557/151‑1 (MQ=255)
                                                           cAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCgccgcc                                                                                        >  1:992854/1‑151 (MQ=255)
                                                                       cgTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGgacgac                                                                            <  1:212913/151‑1 (MQ=255)
                                                                             cGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCg                                                                      >  1:1240944/1‑151 (MQ=255)
                                                                                caccaGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCa                                                                   <  1:550003/151‑1 (MQ=255)
                                                                                    aGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCa                                                               <  2:269116/151‑1 (MQ=255)
                                                                                           ggCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCt                                                        <  1:632283/151‑1 (MQ=255)
                                                                                                    cAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCa                                                 <  2:594406/149‑1 (MQ=255)
                                                                                                    cAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGCGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGa                                               <  2:1219238/151‑1 (MQ=255)
                                                                                                       tggctggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACa                                             >  2:816250/1‑150 (MQ=255)
                                                                                                         gctggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACaaa                                           >  2:257405/1‑150 (MQ=255)
                                                                                                           tggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATc                                        >  1:129280/1‑151 (MQ=255)
                                                                                                            ggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAAt                                         <  1:194591/149‑1 (MQ=255)
                                                                                                            ggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATc                                        <  2:479874/150‑1 (MQ=255)
                                                                                                            ggcGCATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCa                                       <  2:33056/151‑1 (MQ=255)
                                                                                                               gcATGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCt                                    >  2:1180209/1‑151 (MQ=255)
                                                                                                                 aTGGGTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCg                                  >  2:563244/1‑151 (MQ=255)
                                                                                                                    ggTGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCaa                               <  2:1005947/151‑1 (MQ=255)
                                                                                                                      tGATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAAcc                             >  1:14076/1‑151 (MQ=255)
                                                                                                                       gATACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAAcc                             <  1:772032/150‑1 (MQ=255)
                                                                                                                        aTACCGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCtt                           >  1:667962/1‑151 (MQ=255)
                                                                                                                            cGCCAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTc                          <  1:104345/148‑1 (MQ=255)
                                                                                                                              ccAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGgcgc                     <  1:737823/151‑1 (MQ=255)
                                                                                                                               cAAACATCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGgcgc                     >  2:55471/1‑150 (MQ=255)
                                                                                                                                    aTCACATGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTcacc                >  1:514967/1‑150 (MQ=255)
                                                                                                                                         aTGCGACATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCgg           >  1:549098/1‑150 (MQ=255)
                                                                                                                                              aCATGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAAc     >  2:1156149/1‑151 (MQ=255)
                                                                                                                                                 tGGTATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCgg  >  1:338209/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCATTTTCATCGCCACTTCCACCGCTACGGAACCGGAGTCCGCGAGAAAAACGCACTCCAGCGGTTGCGGCGTCATCGCCACCAGTTTGCGGCACAGCTCAATGGCTGGCGCATGGGTGATACCGCCAAACATCACATGCGACATGGCATCAATTTGCGACTTCATCGCCGCATTAAGCTGCGGGTGATTGTAGCCGTGGATCGCCGCCCACCAGGACGACATACCGTCAACCAGGCGTCTGCCGTCAGACAAAATCAGCTCGCAACCTTCGGCGCTCACCACCGGATAAACCGG  >  NC_000913/808890‑809185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑