Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,086,147 G→A A44A (GCG→GCA fdhD → formate dehydrogenase formation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,086,1470GA97.0% 229.1 / ‑3.8 67A44A (GCG→GCAfdhDformate dehydrogenase formation protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (34/30);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATG  >  NC_000913/4086004‑4086292
                                                                                                                                               |                                                                                                                                                 
ggTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCt                                                                                                                                            <  1:1220249/151‑1 (MQ=255)
   tAAGAGAAAGTGAACAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGAGTTCGTCAAATTGAGTTATGGCGGCGTGAGTATTTAAAACACCCACGTATTAATTAGGACGCGGAAAAAGTTGCCGATGCTCTTGTGTa                                                                                                                                           >  1:80391/1‑149 (MQ=255)
     agagAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAAc                                                                                                                                       <  2:554083/151‑1 (MQ=255)
     agagAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAAc                                                                                                                                       <  2:449043/151‑1 (MQ=255)
        gaAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGc                                                                                                                                    <  1:758377/151‑1 (MQ=255)
         aaaGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGc                                                                                                                                    >  2:380808/1‑150 (MQ=255)
           aGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCAtt                                                                                                                                 >  2:358491/1‑151 (MQ=255)
              gAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTc                                                                                                                               <  1:1187103/150‑1 (MQ=255)
                  aaaaCACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCAtg                                                                                                                          >  2:151248/1‑151 (MQ=255)
                   aaaCACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACAACCACGGTTAGATTAGGGCTCGGAAGAAGTTCCCGGTGCACTGGTCTACAACGGAAt                                                                                                                                  >  1:1042754/1‑142 (MQ=255)
                      cacaGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATgtgg                                                                                                                       >  1:450960/1‑150 (MQ=255)
                      cacaGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTGCCCGTTGCACTGGTCTACAACGGCATTTCGCATgtggt                                                                                                                      <  2:1149347/151‑1 (MQ=255)
                      cacaGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGGGATGATTTACAACACCCACGGTTAGATGAGGGCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCCTTTCGCATgtgg                                                                                                                       >  2:122689/1‑150 (MQ=255)
                         aGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGtga                                                                                                                    >  2:96542/1‑150 (MQ=255)
                         aGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGtga                                                                                                                    >  1:436690/1‑150 (MQ=255)
                          gCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAAAACCCACGGTTAGATGAGGTCCCGGAAGAAGTTTCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGtgatg                                                                                                                  >  2:964849/1‑151 (MQ=255)
                           cGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGtgatga                                                                                                                 <  2:90697/151‑1 (MQ=255)
                           cGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGtgatg                                                                                                                  >  1:870761/1‑150 (MQ=255)
                           cGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGtgatg                                                                                                                  >  1:82259/1‑150 (MQ=255)
                             aaaaGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTgatgat                                                                                                                <  1:202738/150‑1 (MQ=255)
                                 gAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGt                                                                                                           <  2:883020/151‑1 (MQ=255)
                                   aaTCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTc                                                                                                          >  2:1095186/1‑150 (MQ=255)
                                    aTCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGc                                                                                                        >  2:298550/1‑151 (MQ=255)
                                        aaaaTGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCa                                                                                                     >  2:501998/1‑150 (MQ=255)
                                            tgtgACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGAc                                                                                                >  2:559186/1‑151 (MQ=255)
                                             gtgACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGAc                                                                                                <  2:146894/150‑1 (MQ=255)
                                              tgACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTTGCCCAAAGACCt                                                                                              >  1:18408/1‑151 (MQ=255)
                                               gACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCtt                                                                                             <  1:161033/151‑1 (MQ=255)
                                               gACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCtt                                                                                             >  1:458530/1‑151 (MQ=255)
                                                aCAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTg                                                                                            <  2:52226/151‑1 (MQ=255)
                                                   aaTATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGt                                                                                         >  2:776331/1‑151 (MQ=255)
                                                        cacaGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACtt                                                                                     <  2:822684/150‑1 (MQ=255)
                                                          caGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTgc                                                                                  >  2:68902/1‑151 (MQ=255)
                                                           aGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTgcg                                                                                 <  2:725014/151‑1 (MQ=255)
                                                             gtgtTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCt                                                                               >  2:511287/1‑151 (MQ=255)
                                                              tgtTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTc                                                                              >  1:145525/1‑151 (MQ=255)
                                                                  cGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGCAGCCGCGGAAGTAGTTTCCGTTGCAATGGTCTACAAGGGCATTTCGCATGTGGGGATGATGGCGTCGCCCAAAGACCTTGAGTACTTGGCGCTCgggt                                                                          >  2:4135/1‑149 (MQ=255)
                                                                   gTCAAATTGAGTTATGGCGGCGTGATGATTTACAACCCCCACGGCTAATTGAGGTCGCGGAAGAAGCTCCCGTTGCGCTGGTCTACAACGGCATTTCCCATGTGGTGATGATGGCGTCGCCCAAAGACCATGAGTAATTTGCGCTCGGttt                                                                         >  1:593242/1‑151 (MQ=255)
                                                                      aaaTTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTc                                                                      <  1:907921/151‑1 (MQ=255)
                                                                       aaTTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCg                                                                     <  2:1179252/151‑1 (MQ=255)
                                                                        aTTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCg                                                                     >  1:287387/1‑150 (MQ=255)
                                                                        aTTGAGTTATGGCGGCGGGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCCTGAGTACTTTGCGGTCGGTTTTTc                                                                      >  1:831027/1‑149 (MQ=255)
                                                                          tGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCtt                                                                  >  2:1197670/1‑151 (MQ=255)
                                                                          tGAGTTATGGCGGCGGGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCtt                                                                  >  2:1116280/1‑151 (MQ=255)
                                                                            aGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCttt                                                                 >  1:21426/1‑150 (MQ=255)
                                                                             gTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTcc                                                               <  1:428160/151‑1 (MQ=255)
                                                                             gTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTcc                                                               >  2:124675/1‑151 (MQ=255)
                                                                               tATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCg                                                              <  2:1002483/150‑1 (MQ=255)
                                                                                aTGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAg                                                            <  1:780462/151‑1 (MQ=255)
                                                                                  ggcggcGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAggg                                                          >  1:1027305/1‑151 (MQ=255)
                                                                                   gcggcgTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAgggg                                                         <  1:583461/151‑1 (MQ=255)
                                                                                            tgatTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGa                                                 >  2:1121488/1‑150 (MQ=255)
                                                                                              atTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAg                                              <  2:842295/151‑1 (MQ=255)
                                                                                               tttACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGt                                             >  1:623352/1‑151 (MQ=255)
                                                                                                ttACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGt                                             <  2:712159/150‑1 (MQ=255)
                                                                                                 tACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTc                                            <  1:477928/150‑1 (MQ=255)
                                                                                                  acaacaCCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTcc                                           <  1:797642/150‑1 (MQ=255)
                                                                                                  acaacaCCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGGGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGccc                                           >  1:276556/1‑150 (MQ=255)
                                                                                                      cacCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCcgcgcg                                      >  1:171636/1‑151 (MQ=255)
                                                                                                          cACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGatat                                  >  2:422859/1‑151 (MQ=255)
                                                                                                          cACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGatat                                  <  1:358482/151‑1 (MQ=255)
                                                                                                                       ggTCGCGGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGAt                     <  2:919802/151‑1 (MQ=255)
                                                                                                                          cgcgGAAGAAGTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGt                   <  1:750408/150‑1 (MQ=255)
                                                                                                                              gaagaaGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGttc              >  2:453208/1‑151 (MQ=255)
                                                                                                                                agaagTTCCCGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGttcct            <  2:463516/151‑1 (MQ=255)
                                                                                                                                       cccGTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGt      <  2:395358/150‑1 (MQ=255)
                                                                                                                                          gTTGCACTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATg  <  1:456305/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GGTTAAGAGAAAGTGAAAAAAACACAGCGAAAAGAAATCGAAAATGTGACAAATATCACAGGTGTTCGTCAAATTGAGTTATGGCGGCGTGATGATTTACAACACCCACGGTTAGATGAGGTCGCGGAAGAAGTTCCCGTTGCGCTGGTCTACAACGGCATTTCGCATGTGGTGATGATGGCGTCGCCCAAAGACCTTGAGTACTTTGCGCTCGGTTTTTCGCTTTCCGAGGGGATTATCGAAAGTCCGCGCGATATCTTCGGCATGGATGTCGTTCCTTCCTGTAATG  >  NC_000913/4086004‑4086292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: