Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	815,771	C→T	A293T (GCG→ACG)	ybhK ←	putative CofD superfamily transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	815,771	0	C	T	100.0%	186.0 / NA	55	A293T (GCG→ACG)	ybhK	putative CofD superfamily transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/30);  total (25/30)

AATATATTGAATTTGCGCGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGCGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAATGACCTTTTTACCAACATACT  >  NC_000913/815625‑815914                                                                                                                                                   |                                                                                                                                                aaTATATTGAATTTGCGCGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGtt                                                                                                                                              >  1:157119/1‑150 (MQ=255) aaTATATTGAATTTGCGCGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTa                                                                                                                                             <  1:333690/151‑1 (MQ=255)    atatTGAATTTGCGCGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATg                                                                                                                                           <  1:928240/150‑1 (MQ=255)    atatTGAATTTGCGCGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATg                                                                                                                                           <  2:362905/150‑1 (MQ=255)    atatTGAATTTGCGCGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATg                                                                                                                                           <  2:145764/150‑1 (MQ=255)      atTGAATTTGCGCGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTa                                                                                                                                         <  1:1235166/150‑1 (MQ=255)         gAATTTGCGCGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTaaca                                                                                                                                      <  2:208200/150‑1 (MQ=255)          aatttaaggggTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCCCTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACGTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTaacaac                                                                                                                                    <  1:41273/142‑1 (MQ=37)            tttGCGCGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTg                                                                                                                                  >  1:431758/1‑151 (MQ=255)                 gcgGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGt                                                                                                                             >  2:1214611/1‑151 (MQ=255)                   ggTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCa                                                                                                                           <  1:110097/151‑1 (MQ=255)                        gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGAc                                                                                                                       <  1:1262037/150‑1 (MQ=255)                              cGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACgg                                                                                                                >  1:1171335/1‑151 (MQ=255)                              cGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACgg                                                                                                                >  1:676129/1‑151 (MQ=255)                                 aTAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAat                                                                                                             >  1:127834/1‑151 (MQ=255)                                  tAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAat                                                                                                             >  1:725163/1‑150 (MQ=255)                                    aGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATc                                                                                                          >  2:872803/1‑151 (MQ=255)                                     ggCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATc                                                                                                          >  1:855373/1‑150 (MQ=255)                                        gTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTg                                                                                                      >  2:671804/1‑151 (MQ=255)                                         ttCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTg                                                                                                      <  1:1237384/150‑1 (MQ=255)                                          tCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGc                                                                                                    >  1:188916/1‑151 (MQ=255)                                               acgcATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTcc                                                                                                >  1:232552/2‑150 (MQ=255)                                                   aTCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTAc                                                                                           <  1:402996/151‑1 (MQ=255)                                                   aTCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTAc                                                                                           <  2:1146068/151‑1 (MQ=255)                                                            aaaCAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGAt                                                                                  <  1:55770/151‑1 (MQ=255)                                                                 acGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACaa                                                                             <  2:1094647/151‑1 (MQ=255)                                                                 acGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACaa                                                                             <  2:1011319/151‑1 (MQ=255)                                                                       aCTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGc                                                                        >  2:1209643/1‑150 (MQ=255)                                                                          ttGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGctct                                                                     <  2:869955/150‑1 (MQ=255)                                                                                 cAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCAccgc                                                             <  2:384086/151‑1 (MQ=255)                                                                                 cAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCAccgc                                                             >  2:718974/1‑151 (MQ=255)                                                                                 cAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCAccgc                                                             <  2:683187/151‑1 (MQ=255)                                                                                      ggAAGCCGGAATTTTCCTTCGGATTCCCTTTAACCAAAAGCCTGTAACCCCTTTTCCAGCGTGTTATGTAACAACGGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGACCACAACCCTCTTTTTCACCGCCgag                                                         <  1:671223/150‑1 (MQ=255)                                                                                           ccGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATcc                                                   >  1:973720/1‑151 (MQ=255)                                                                                             ggAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCAc                                                 <  2:596289/151‑1 (MQ=255)                                                                                                 ttttCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACttt                                              <  1:562473/150‑1 (MQ=255)                                                                                                   ttCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTgg                                           >  1:247825/1‑151 (MQ=255)                                                                                                     ccTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGcc                                         >  2:626969/1‑151 (MQ=255)                                                                                                         cGGATTTCCGTTAACCAAAAGCCGGTACCCCCTTTTCCAGGGTGTTATGTAACAACTGGCGGTAAGAACGATACGGAATACCGCTGCCCCCCAGTCCCCCCGGGATCACATCCCGCTTTTTCACCGCCGAGACATCCACTTTTGGCCcg                                       <  1:219616/149‑1 (MQ=255)                                                                                                             tttCCGTTAACCAAAAGCCTGTACCGCCTGTTCCAGCGTGTTATGAAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGAt                                  <  2:712275/150‑1 (MQ=255)                                                                                                                ccGTTAACCTAAAGCCTGAAACGCCTTTCCCAGCGTGTTATGTAACAACGGGCGGTCATGACGATCCGAAATATCGCTGGCCCCCAGTACCTCCTGGATCACAACCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGAcc                              <  1:78883/151‑1 (MQ=255)                                                                                                                  gTTAACCTAAAGCCTGAAACGCCTTTCCCAGGGTGTTTTGTAACAACTGGCGGTCATGACGATAGGGAATCTCGCTGGCCTCCAGTCCCTCCTGGACCACATTCCGCTCTTCCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCg                             <  2:400289/150‑1 (MQ=255)                                                                                                                    tAACCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCAt                          >  1:705518/1‑151 (MQ=255)                                                                                                                     acccTAAAGCCTTTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCAGGACCATACGAAATACCGCGGGCCTCCATTCCCACCGGGATCACAACCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATc                         <  2:1185096/149‑1 (MQ=255)                                                                                                                     acccTAAACCCTGTACCCCTTTTCCCAGCGTGTTATAAAAAAAGGGCCGGTCATAACGATAGGGATTATCGCGGGCCTCCAGTCCCTCCGGAATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCAt                          <  1:829963/148‑1 (MQ=255)                                                                                                                      aCCTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTTGCCCGACGATGACCGCAt                          >  1:1087945/1‑149 (MQ=255)                                                                                                                        cTAAAGCCTGTAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAAt                      >  1:1197602/1‑151 (MQ=255)                                                                                                                                  tAACGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTCCCTCCTGGATCAAAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAATGACCttttt             <  2:60510/150‑1 (MQ=255)                                                                                                                                   acggctttttCCAGGTTGTTATGTAATATCGGGGGTTCTGGTGGATTCGAAATATCGCTGACCTCCAGTCCCTTTGGGAACAAAATCCCCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAATGACCtttttcc           <  2:1071455/145‑3 (MQ=255)                                                                                                                                   aaCGCCTTTTCCGGCGTGTTTTGGAACAACTGGCGGTCATGACCATACGGAATATCGTTGGCCTCCAGTCCCTCCGGGATCTCAATCCGCCCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAATGCCCTTTTTAc           <  2:454590/151‑1 (MQ=255)                                                                                                                                   aaCGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAATGACCTTTTTAc           >  2:623834/1‑151 (MQ=255)                                                                                                                                     cGCCTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAATGACCTTTTTAcc          >  2:848710/1‑150 (MQ=255)                                                                                                                                        cTTTTCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAATGACCTTTTTACCAAc       >  1:776752/1‑150 (MQ=255)                                                                                                                                         ttttCCAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAATGACCTTTTTACCAACAt     >  1:144530/1‑151 (MQ=255)                                                                                                                                             ccAGCGTGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAATGACCTTTTTACCAACATACt  >  1:971491/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                AATATATTGAATTTGCGCGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGCGCACTTTGTCTTCAATAGGAAGCCGGAATTTTCCTTCGGATTTCCGTTAACCTAAAGCCTGTAACGCCTTTTCCAGCGCGTTATGTAACAACTGGCGGTCATGACGATACGGAATATCGCTGGCCTCCAGTACCTCCTGGATCACAATCCGCTCTTTCACCGCCGAGACATCCACTTTTGGCCCGACGATGACCGCATCAATGACCTTTTTACCAACATACT  >  NC_000913/815625‑815914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑