Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,198,201 C→T E13K (GAA→AAA)  ymfE ← e14 prophage; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,198,2010CT97.5% 279.2 / ‑1.2 79E13K (GAA→AAA) ymfEe14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (31/46);  total (32/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

GCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTA  >  NC_000913/1198053‑1198342
                                                                                                                                                    |                                                                                                                                             
gCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTAt                                                                                                                                             >  2:190641/1‑151 (MQ=255)
gCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTAt                                                                                                                                             >  2:81959/1‑151 (MQ=255)
  ccAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTttatta                                                                                                                                           <  2:414750/151‑1 (MQ=255)
   cAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTtattat                                                                                                                                          >  1:484681/1‑151 (MQ=255)
   cAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTtattat                                                                                                                                          <  2:518134/151‑1 (MQ=255)
    aaTTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTtattat                                                                                                                                          >  2:84046/1‑150 (MQ=255)
     aTTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATaa                                                                                                                                        <  2:761275/151‑1 (MQ=255)
         ataaAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAtttt                                                                                                                                    <  2:1001495/151‑1 (MQ=255)
          taaAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAttttt                                                                                                                                   >  2:761651/1‑151 (MQ=255)
           aaaGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAttttt                                                                                                                                   <  2:831690/150‑1 (MQ=255)
           aaaGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAttttt                                                                                                                                   <  1:843516/150‑1 (MQ=255)
             aGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAttttt                                                                                                                                   <  1:774374/148‑1 (MQ=255)
                     tGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCg                                                                                                                         >  2:681627/1‑150 (MQ=255)
                      gATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGa                                                                                                                        <  1:1186326/150‑1 (MQ=255)
                           ttAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAGTAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAAc                                                                                                                      <  2:193865/147‑1 (MQ=255)
                           ttAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATCCAATCTATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGCGGGTTCGAACat                                                                                                                    <  1:360569/149‑1 (MQ=255)
                            tAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACatat                                                                                                                  <  1:498116/150‑1 (MQ=255)
                                  gTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCAc                                                                                                           >  2:1245963/1‑151 (MQ=255)
                                          tACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTc                                                                                                    >  2:460726/1‑150 (MQ=255)
                                           aCTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCAc                                                                                                  >  2:1104501/1‑151 (MQ=255)
                                            cTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACg                                                                                                 >  2:937406/1‑151 (MQ=255)
                                                   ggtAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAgg                                                                                          <  1:75969/151‑1 (MQ=255)
                                                   ggtAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAg                                                                                           <  1:690279/150‑1 (MQ=255)
                                                     tAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGa                                                                                         >  2:244379/1‑150 (MQ=255)
                                                     tAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGAt                                                                                        >  2:323063/1‑151 (MQ=255)
                                                       aTGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGAt                                                                                        >  1:70488/1‑149 (MQ=255)
                                                         gTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAgt                                                                                     <  1:320495/150‑1 (MQ=255)
                                                            attaAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTaa                                                                                 <  2:923376/151‑1 (MQ=255)
                                                            attaAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTa                                                                                  <  1:244375/150‑1 (MQ=255)
                                                               aaaGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATgg                                                                              >  1:851584/1‑151 (MQ=255)
                                                               aaaGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATgg                                                                              >  1:332381/1‑151 (MQ=255)
                                                                aaGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGt                                                                             <  1:225943/151‑1 (MQ=255)
                                                                  gggATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTaa                                                                           <  2:272332/151‑1 (MQ=255)
                                                                  gggATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTa                                                                            >  1:238277/1‑150 (MQ=255)
                                                                   ggATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAat                                                                          <  1:383699/151‑1 (MQ=255)
                                                                     aTGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAatat                                                                        <  1:465463/151‑1 (MQ=255)
                                                                     aTGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAatat                                                                        >  2:328138/1‑151 (MQ=255)
                                                                     aTGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAatat                                                                        <  1:963425/151‑1 (MQ=255)
                                                                     aTGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGAATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAata                                                                         >  1:972113/1‑150 (MQ=255)
                                                                      tGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATAtt                                                                       >  1:132163/1‑151 (MQ=255)
                                                                       gTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATAttt                                                                      >  2:790046/1‑151 (MQ=255)
                                                                         aTTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATtta                                                                     >  1:81898/1‑150 (MQ=255)
                                                                          ttCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaat                                                                   >  2:1196738/1‑151 (MQ=255)
                                                                            cGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaatta                                                                 <  1:1098833/151‑1 (MQ=255)
                                                                            cGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaatta                                                                 <  1:1032549/151‑1 (MQ=255)
                                                                               aaaTAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAgt                                                              <  2:851622/151‑1 (MQ=255)
                                                                               aaaTAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAgt                                                              <  2:502698/151‑1 (MQ=255)
                                                                                 aTAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAgtgt                                                            >  2:741431/1‑151 (MQ=255)
                                                                                 aTAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAgtgt                                                            <  2:197485/151‑1 (MQ=255)
                                                                                    aCTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCAt                                                         <  1:829134/151‑1 (MQ=255)
                                                                                      tAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCAtt                                                        >  2:1253037/1‑150 (MQ=255)
                                                                                       aGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGTTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATtat                                                      <  1:1095704/151‑1 (MQ=255)
                                                                                       aGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCAtt                                                        <  2:1059725/149‑1 (MQ=255)
                                                                                       aGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCAtt                                                        <  1:335454/149‑1 (MQ=255)
                                                                                             aTGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACt                                                 <  2:1200008/150‑1 (MQ=255)
                                                                                             aTGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACt                                                 <  2:926758/150‑1 (MQ=255)
                                                                                                 aaTATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTc                                             >  1:338118/1‑150 (MQ=255)
                                                                                                 aaTATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTc                                             <  1:840012/150‑1 (MQ=255)
                                                                                                  atatatTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAg                                           <  2:284476/151‑1 (MQ=255)
                                                                                                       ttAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgt                                      >  2:519455/1‑151 (MQ=255)
                                                                                                        tAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgt                                      <  1:80871/150‑1 (MQ=255)
                                                                                                         aGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgtt                                     <  2:363930/150‑1 (MQ=255)
                                                                                                         aGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgtt                                     <  1:831114/150‑1 (MQ=255)
                                                                                                           cGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgttagt                                  <  1:105297/151‑1 (MQ=255)
                                                                                                            gTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgttagtt                                 <  2:1246760/151‑1 (MQ=255)
                                                                                                                ttAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACa                              <  2:516001/150‑1 (MQ=255)
                                                                                                                ttAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACa                              >  2:58205/1‑150 (MQ=255)
                                                                                                                  aaGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAAtt                           >  1:870734/1‑151 (MQ=255)
                                                                                                                  aaGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAAt                            <  1:1001689/150‑1 (MQ=255)
                                                                                                                  aaGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAAt                            <  2:757878/150‑1 (MQ=255)
                                                                                                                     aaaGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAAttttt                        <  1:127178/151‑1 (MQ=255)
                                                                                                                     aaaGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAAttttt                        >  2:465828/1‑151 (MQ=255)
                                                                                                                       aGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCCGTAGGTTAGTTACAATTTTTTg                      >  2:101908/1‑150 (MQ=255)
                                                                                                                           gCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGAGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGATAGTTACAATTTTTTGTATc                  >  2:42797/1‑151 (MQ=255)
                                                                                                                                   aaGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAgg          <  2:12572/151‑1 (MQ=255)
                                                                                                                                   aaGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAgg          <  2:627488/151‑1 (MQ=255)
                                                                                                                                    aGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGc         <  1:475949/151‑1 (MQ=255)
                                                                                                                                    aGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGc         >  1:298268/1‑151 (MQ=255)
                                                                                                                                      tttAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCt        <  2:1183525/150‑1 (MQ=255)
                                                                                                                                       ttAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTg       >  1:25748/1‑150 (MQ=255)
                                                                                                                                        tAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGa      <  2:48084/150‑1 (MQ=255)
                                                                                                                                           gCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCt   >  1:373725/1‑150 (MQ=255)
                                                                                                                                           gCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTa  <  2:767894/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTA  >  NC_000913/1198053‑1198342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: