Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,410,0450T.98.1% 470.6 / 4.8 104intergenic (+39/‑88)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base . (45/56);  total (47/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

Only 100 of 113 total aligned reads displayed.

AACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACAAAATAT  >  NC_000913/4409897‑4410191
                                                                                                                                                    |                                                                                                                                                  
aaCATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttt                                                                                                                                                  <  2:8740/151‑1 (MQ=255)
aaCATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttt                                                                                                                                                  >  1:1241582/1‑151 (MQ=255)
 aCATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttt                                                                                                                                                 >  1:412698/1‑151 (MQ=255)
 aCATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttt                                                                                                                                                 >  2:1103471/1‑151 (MQ=255)
  cATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGTGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttt                                                                                                                                                <  2:826050/151‑1 (MQ=255)
         aTGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtct                                                                                                                                         <  1:742876/151‑5 (MQ=255)
          tGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtct                                                                                                                                         >  2:465440/1‑146 (MQ=255)
          tGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtct                                                                                                                                         >  1:320436/1‑146 (MQ=255)
              ttGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGc                                                                                                                                   >  1:1060830/1‑151 (MQ=255)
               tGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGc                                                                                                                                   <  2:1305833/150‑1 (MQ=255)
               tGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGc                                                                                                                                   <  2:14621/150‑1 (MQ=255)
                gATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCt                                                                                                                                  <  2:1006543/150‑1 (MQ=255)
                 aTCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTgg                                                                                                                                >  2:817441/1‑151 (MQ=255)
                 aTCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTgg                                                                                                                                >  1:603598/1‑151 (MQ=255)
                   cAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGt                                                                                                                               >  2:341904/1‑150 (MQ=255)
                    aGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGt                                                                                                                               >  1:364863/1‑150 (MQ=255)
                    aGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTaa                                                                                                                             <  1:1361081/151‑1 (MQ=255)
                    aGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTaa                                                                                                                             <  2:58368/151‑1 (MQ=255)
                      cATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTa                                                                                                                              >  2:825474/1‑148 (MQ=255)
                         cccGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATaa                                                                                                                        <  2:1298929/151‑1 (MQ=255)
                         cccGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATa                                                                                                                         <  2:897257/150‑1 (MQ=255)
                          ccGATGGCTGGAAGCGTTTCTTCCCTGGACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAt                                                                                                                       >  1:1154353/1‑151 (MQ=255)
                          ccGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAt                                                                                                                       <  1:1158213/151‑1 (MQ=255)
                           cGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                                                                      >  2:226817/1‑151 (MQ=255)
                            gATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                                                                      >  2:823915/1‑150 (MQ=255)
                                  tGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAtt                                                                                                               >  2:1073091/1‑151 (MQ=255)
                                   ggATGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGCAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTCCGTAtt                                                                                                               <  1:96006/150‑1 (MQ=255)
                                       gCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaaa                                                                                                          <  2:659953/151‑1 (MQ=255)
                                        cGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCATAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAat                                                                                                         <  1:122692/151‑1 (MQ=255)
                                         gTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAat                                                                                                         >  2:854203/1‑150 (MQ=255)
                                         gTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAat                                                                                                         >  1:721802/1‑150 (MQ=255)
                                          tttCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAatat                                                                                                       >  1:367397/1‑151 (MQ=255)
                                            tcttcCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTc                                                                                                     >  1:854794/1‑151 (MQ=255)
                                            tcttcCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGGATTg                                                                                                              >  1:226273/1‑142 (MQ=255)
                                                cccTGAACGTTTCGGTTGCGACCGAAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTAAAGCTGGTAAGATAATTTCGTATTGCAAATATTCa                                                                                                    <  1:1039757/148‑1 (MQ=255)
                                                    gAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                                                                             <  2:482834/151‑1 (MQ=255)
                                                    gAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat                                                                                              <  2:1300936/150‑1 (MQ=255)
                                                     aaCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatta                                                                                            <  2:1126450/151‑1 (MQ=255)
                                                       cGTTTCGGTTGCGACCAGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTGTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatta                                                                                            <  2:516754/149‑1 (MQ=255)
                                                        gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                                                                         <  1:42682/151‑1 (MQ=255)
                                                          ttccggtGCGACCGGAATTTGCTTATATGAAGCGGTGCACCAGCGCAGCTAATTTCTCAGAATTATGACAAGCCATCTCGTTTTGGATGGTTTTTTTTGGCTATAGCTGGTAAGAGAATTACGTATTGCGAATATTCACTTTATTAATAtt                                                                                       >  1:443774/7‑151 (MQ=255)
                                                          ttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAatat                                                                                        <  2:216759/150‑1 (MQ=255)
                                                          ttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAtt                                                                                       >  2:1334673/1‑151 (MQ=255)
                                                          ttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAtt                                                                                       >  2:1115616/1‑151 (MQ=255)
                                                           tCGGTTGCGACGGGAATGTGCTTATTTGAAGAGGTGCGCCAGCGCAGCTAATTTGTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATCGCTGGTAAGATAATTACGTATTGAAAGTATTCATTTTATTAATCttt                                                                                      >  1:346416/1‑151 (MQ=255)
                                                           tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAttt                                                                                      >  2:941057/1‑151 (MQ=255)
                                                              gTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATTCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                                                                                   <  1:868027/151‑1 (MQ=255)
                                                              gTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                                                                                   <  1:369889/151‑1 (MQ=255)
                                                                  cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACtt                                                                                <  1:633984/151‑1 (MQ=255)
                                                                  cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTa                                                                               <  1:1024300/151‑1 (MQ=255)
                                                                      cGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                                                                           >  1:1020819/1‑151 (MQ=255)
                                                                      cGGAATTTGCTTATGTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                                                                            <  1:1120928/150‑1 (MQ=255)
                                                                        gAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGGCATCCAGGTTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                                                                         >  1:1069223/1‑151 (MQ=255)
                                                                             tGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATgg                                                                     <  1:34584/150‑1 (MQ=255)
                                                                                 tATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaata                                                                 >  2:888090/1‑150 (MQ=255)
                                                                                     tGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat                                                             >  2:1011749/1‑150 (MQ=255)
                                                                                      gAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat                                                           >  2:418020/1‑151 (MQ=255)
                                                                                      gAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat                                                           <  1:135061/151‑1 (MQ=255)
                                                                                      gAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat                                                           <  2:1156066/151‑1 (MQ=255)
                                                                                       aaGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg                                                          >  2:20223/1‑151 (MQ=255)
                                                                                        aGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGa                                                         <  1:347432/151‑1 (MQ=255)
                                                                                        aGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGa                                                         <  2:301014/151‑1 (MQ=255)
                                                                                         gCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAc                                                        >  2:90125/1‑151 (MQ=255)
                                                                                          cGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACa                                                       <  1:419407/151‑1 (MQ=255)
                                                                                           ggTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg                                                          <  1:610291/147‑1 (MQ=255)
                                                                                            gTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATg                                                     >  2:621623/1‑151 (MQ=255)
                                                                                            gTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATg                                                     <  2:584974/151‑1 (MQ=255)
                                                                                                gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGaa                                                  <  1:415239/150‑1 (MQ=255)
                                                                                                gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAAt                                                 >  2:630475/1‑151 (MQ=255)
                                                                                                 ccAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATc                                                >  1:548825/1‑151 (MQ=255)
                                                                                                  cAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATcc                                               >  2:1250308/1‑151 (MQ=255)
                                                                                                  cAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATcc                                               <  1:70834/151‑1 (MQ=255)
                                                                                                  cAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATcc                                               <  2:67969/151‑1 (MQ=255)
                                                                                                      gcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTg                                           >  2:1135425/1‑151 (MQ=255)
                                                                                                         gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGgcg                                        >  2:570387/1‑151 (MQ=255)
                                                                                                            aaTTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCt                                      <  2:1060898/150‑1 (MQ=255)
                                                                                                              cttCTCAGAATTATGAAGAGACAACCAGATTTGGAGGGATTGTTTTGTCTAGAGCTGGTAGGATACTGACGGATTGCAAATATTCATTTTACTAATATTTATACTGAATCTATATTGATTAATATATGACATGGAATCCGTTGGCGCTa                                     >  1:732414/2‑149 (MQ=255)
                                                                                                                  tcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACg                               >  1:538498/1‑151 (MQ=255)
                                                                                                                   cAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACgg                              <  2:1341847/151‑1 (MQ=255)
                                                                                                                   cAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACgg                              >  1:393796/1‑151 (MQ=255)
                                                                                                                      aaTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGgcgc                           <  2:474490/151‑1 (MQ=255)
                                                                                                                       aTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCt                          <  1:623146/151‑1 (MQ=255)
                                                                                                                        ttATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATCGCTGGACAGATAATTAAGAATTGCAAATATTTATTTTTTTAATATTTAAACTTAATTTATAAGGAATAATATATGATATGGAATCCGTTGGCGCTAGCGACGGCGCt                          >  1:888924/1‑150 (MQ=255)
                                                                                                                        ttATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCt                          >  1:833109/1‑150 (MQ=255)
                                                                                                                        ttATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTg                         >  2:892932/1‑151 (MQ=255)
                                                                                                                          aTGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGc                        >  1:285622/1‑150 (MQ=255)
                                                                                                                               aaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGgcg                                        <  2:1216939/129‑1 (MQ=255)
                                                                                                                               aaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTg                  >  1:362430/1‑151 (MQ=255)
                                                                                                                                 gCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGt                 >  2:187306/1‑150 (MQ=255)
                                                                                                                                    aTCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCt             <  2:21583/151‑1 (MQ=255)
                                                                                                                                      ccAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTg            >  1:748323/1‑150 (MQ=255)
                                                                                                                                      ccAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTg            >  1:395212/1‑150 (MQ=255)
                                                                                                                                      ccAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTg            <  2:426365/150‑1 (MQ=255)
                                                                                                                                      ccAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTg            <  2:1241664/150‑1 (MQ=255)
                                                                                                                                       cAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGaa          <  2:1015888/151‑1 (MQ=255)
                                                                                                                                        aGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAAc         >  2:560991/1‑151 (MQ=255)
                                                                                                                                          aTTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACaa       <  1:349307/151‑1 (MQ=255)
                                                                                                                                            ttGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACaaa      <  1:465419/150‑1 (MQ=255)
                                                                                                                                            ttGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACaaa      <  2:1050147/150‑1 (MQ=255)
                                                                                                                                               gATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATCTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACAAAatat  <  1:216797/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AACATTGCGATGAGTTGATCAGCATCCCGATGGCTGGAAGCGTTTCTTCCCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATGGAATCCGTTGGCGCTAGCGACGGCGCTGCAAACTGTACCTGAACAAAATAT  >  NC_000913/4409897‑4410191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: