Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,125	0	T	A	37.8%	104.6 / 43.6	75	intergenic (‑330/+60)	ybgD/gltA	putative fimbrial‑like adhesin protein/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (36/10);  new base A (0/28);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 2.55e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

ATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATT  >  NC_000913/752977‑753275                                                                                                                                                          |                                                                                                                                                        aTTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATaa                                                                                                                                                       <  1:729585/151‑1 (MQ=255)  ttttCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGATAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATaa                                                                                                                                                       >  1:941809/1‑150 (MQ=255)    ttCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATaaaa                                                                                                                                                     <  1:811168/150‑1 (MQ=255)      cATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑Tc                                                                                                                                                 >  2:91598/1‑151 (MQ=255)       aTAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCa                                                                                                                                                <  2:1258151/151‑1 (MQ=255)                     gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGa                                                                                                                                   >  2:93805/1‑150 (MQ=255)                      ctaCTACAATTAAGTTTTTAATTGAAATTATGGGTTTGCTAATATTTTGAGCTTTTTTTTTTTTTTTAAATTTTTATATAGTTTTGTT‑AATG‑GTTTTTTTTTTTACAATTTTT‑TGTGTTT‑AGG‑CATAAAAAAA‑CAAACCCGCCATATGaa                                                                                                                                  <  1:1419027/148‑1 (MQ=255)                          tACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAAcggcg                                                                                                                             >  2:741500/1‑151 (MQ=255)                             cATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCggg                                                                                                                           >  2:933864/1‑150 (MQ=255)                              aTGAATTTTTTATTTGAAGGTATTGGTTTGGTAAAATTTTGAGCTTTTTTATTCTTTTAAAATATCTTTATATCTTTATA‑AATG‑GATTTTATTTTTA‑AATTTTT‑TGTTTTTAAGG‑CATATAAAAC‑CAACCCCGCCATATGAACGGCGGGtt                                                                                                                         <  1:600570/151‑1 (MQ=255)                               tGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGtt                                                                                                                         >  2:1095888/1‑150 (MQ=255)                                 atttttttAATTAAAAGTTTTGGTTTGTTAATATTTTGAGTTTTTTTTTTTTTTTAAAATATCTATATAGTTTTTTA‑AATG‑GTTTTTTTTTTTACAATTTTT‑TGTGTTT‑AGC‑AATAAAAAAA‑CCACCCCGCCATATGAAcggc                                                                                                                              <  1:137152/140‑1 (MQ=255)                                 atgTTTTTATTTAAAATTTTTGGGTGGTTGTTAATTTGCGTTGTTTTTTTCTTTTTAAATTTCTA‑AAAAGTTTTTT‑AATGATATTTTTTTTTTAAATTTTTT‑TGTGTTT‑GAT‑CATAAAAAAA‑ACAACCCGCCATATGAACGGCGGGTTa                                                                                                                        <  1:691887/147‑1 (MQ=255)                                    tttttATTTAAAGTTATTGGGTGGTTGATATTTTGGGTTTTTTTATTCTTTTTAAATATTTATATAGGTCTGTA‑AATG‑TATTTTTTTTTTAAAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑ACACCCCGCCATATGAACGGCGGGtt                                                                                                                         <  1:558618/145‑1 (MQ=255)                                    tttttAATTGAAAGTATTGGTGTTCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑ATTG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCggg                                                                                                                           >  1:560795/1‑143 (MQ=255)                                    tttttAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGGTaaaa                                                                                                                     >  1:1188812/1‑149 (MQ=255)                                    tttttAATTAAAAGTTTTGGGTTGCTAATAATTTGAGCTTTTCTTTTTTTTTAAAATATTTATATAGTTCTTTT‑AATG‑GTTTTTATTTTTACAATTTTT‑TGTGTTA‑AGG‑AAAAAAAAAA‑CCAACCCGCCATATGAACGGCGGGTTAAAat                                                                                                                    <  1:1407726/150‑1 (MQ=255)                                              aaaGTATTGGGTTGCTGATATTTTGAGCTTTTCTTTTTTTTTTAAATATCTATATAGGTCTTTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTTTTT‑AGG‑AATAAAAAAA‑CCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACtt                                                                                                         <  2:949374/151‑1 (MQ=255)                                               aaGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTTAAATAttttaaaatt                                                                                                         >  1:343205/1‑143 (MQ=255)                                                aatATGGGTTTTCTTATATTTTTTGCTTTTCTTTTCTTTTTAAATTTATTTAAAGGT‑TGTT‑AAAT‑GATTTTTTTTTTTTTATTTTT‑TTTTTTTGATA‑AATATAAAAA‑TAACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACt                                                                                                          <  1:737133/146‑1 (MQ=255)                                                    ttGGGTTGTTGATATTTTGGGTTGTTCTATTTTTTTTAAATTTCTATATAGTTCTGTT‑AATG‑GATTTTATTTTTACATTTTTT‑TGTGTTT‑AGG‑AATAAAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAg                                                                                                       <  2:1162920/147‑1 (MQ=255)                                                    ttGGGTTGCTGATATTTTGAGTTTTTCTATTTTTTTTAAATATTTATATAGGTTTTTT‑AATG‑GATTTTATTTTTACATTTTTT‑TGTGTTT‑AGG‑AATAAAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCaa                                                                                                    <  2:93549/150‑1 (MQ=255)                                                      gggTGGATGTTAATTTGTGTTTTTCTATTTTTTTTAAATATTTATATA‑GTTTGTTAAAAG‑GTTTTTATTTTTACAATTTTT‑TTTTTTT‑AGA‑CAAAAAAAAA‑CCACACCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCa                                                                                                 <  1:1303658/151‑1 (MQ=255)                                                               aTAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAACAATCAACCATTaa                                                                                         >  1:1199037/1‑150 (MQ=255)                                                                tAATTTGAGTTTTTTTTTTTTTTTAAAATATCTATATAGTTCTTTT‑AATG‑GTTTTTTTTTTTAAAATTTTT‑TGTGTTT‑AGG‑CAAAAAAAAA‑CCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAAc                                                                                        <  1:114151/150‑1 (MQ=255)                                                                    ttGAGCTTTTTTTTTTTTTTTAAATATCTATATAGTTCTTTA‑AATG‑GTTTTTTTTTTTAAATTTTTT‑TGTTTTT‑AGG‑CATAAAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCtt                                                                                    <  2:578791/150‑1 (MQ=255)                                                                      gttctttttttttCTTTTAAAATTTTTTTATAGTTTTGTT‑AATG‑GATTTTTTTTTTAAATTTTTT‑TGTTTTT‑AGA‑CATAAAAAAA‑GCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCtt                                                                                    <  2:761478/137‑1 (MQ=255)                                                                       agtttttCTTTTTTTTTTAAATATTTATATAGGTTTTTA‑AATG‑GTTTTTATTTTAAAAATTTTT‑TGTTTTT‑AGG‑AAAAAAAAAA‑CCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGa                                                                                  <  2:1008309/144‑1 (MQ=255)                                                                       aGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTg                                                                                   >  1:550491/1‑148 (MQ=255)                                                                         ttGTTTTTTTTTTTTTAAATATTTATATAGTTTTTTA‑AATG‑GTTTTTTTTTTTAAATTTTTT‑TTTTTTT‑AGC‑AAAAAAAAAA‑CCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                               <  1:1265477/149‑1 (MQ=255)                                                                          ttttCTTTTTTTTTTAAATATTTATATAGGTCTGTT‑AATG‑GATTTTTTTTTTACAATTTTT‑TGTTTTT‑AGG‑AATAAAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                               <  2:885036/147‑1 (MQ=255)                                                                            ttCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAACAATCAACCATTAAACCCTGATATCg                                                                             >  1:460469/1‑149 (MQ=255)                                                                               ttttttttttAAATTTCTATATTGTTTTGTT‑AAT‑‑GTTTTTGTTTTTAAAATTTTT‑TTGTTTT‑AAG‑CAAAAAAAAATTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCtt                                                                          <  1:893559/144‑1 (MQ=255)                                                                               tcttCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AAG‑CATATAAAAA‑TCAACCCACAATATGCACGGCGGGTTAAATTATTTTCCATTTTAAATTCAACCATAAACCCTTGGTATCCCttt                                                                         >  1:207335/3‑150 (MQ=255)                                                                               tATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCtttt                                                                        >  2:1103860/1‑151 (MQ=255)                                                                               tATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAAACATTAACGCTTGATATCGCtttt                                                                        >  1:586204/1‑151 (MQ=255)                                                                                aTTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCtttt                                                                        >  2:1395801/1‑150 (MQ=255)                                                                                  tgttttaAAATATTAATATAGGGTTTAT‑AATG‑AATTTTATTTTTAAATTTTTT‑TGTTTTT‑AGC‑AAAAAAAAAA‑CCAAACCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCtttt                                                                        <  1:466727/146‑1 (MQ=255)                                                                                  tCTTTTTAAATATCTATATAGGTCTTTT‑AATG‑GTTTTTATTTTTAAATTTTTT‑TGTGTTT‑AGG‑CATAAAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTaa                                                                      <  2:705147/150‑1 (MQ=255)                                                                                       taaaaTATTTATATAGGTTTGTA‑AATG‑GTTTTTATTTTTACATTTTTT‑TGTGTTT‑AGG‑AATAAAAAAA‑TCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTcg                                                                 <  2:153603/148‑1 (MQ=255)                                                                                         aaaTATCTATATAGGTTTTTA‑AATG‑TTTTTTTTTTTTAAATTTTTT‑TGT‑TTT‑AGGTAATATAAAAA‑CCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTcg                                                                 <  1:748471/148‑1 (MQ=255)                                                                                             atttatataGGTCTTTT‑AAGG‑GATTTTTTTTTTAAATTTTTT‑GGTGTTT‑AGG‑CATAAAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                                           <  2:1193913/147‑1 (MQ=255)                                                                                                tatataGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                                        >  2:1395480/1‑150 (MQ=255)                                                                                                   ataGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTAAAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                                      >  2:70103/1‑149 (MQ=255)                                                                                                   ataGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAAAATTTACAACTTAGCAATCAAACATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTtata                                                      >  2:459596/1‑149 (MQ=255)                                                                                                     agttgtttt‑AATG‑GTTTTTTTTTTTACAATTTTT‑TTTTTTA‑AGG‑CATAAAAAAA‑TAACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                                        <  1:649852/138‑1 (MQ=255)                                                                                                      ggTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTTAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTTAAGTCGCGTTTTTTATATcc                                                   >  2:982/1‑149 (MQ=255)                                                                                                      ggTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTg                                                 >  2:668220/1‑151 (MQ=255)                                                                                                      ggTCTGTT‑AATG‑AATTTTATTTTTAAATTTTTT‑TGTGTTT‑AGG‑AATAAAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTg                                                 <  2:261536/151‑1 (MQ=255)                                                                                                        ttttttaaaTG‑TATTTTATTTTTATATTTTTT‑TTTTTTA‑AGT‑TTTATAAAAA‑ACACCCCGCCATATGAACGGCGGGTTAAAATATTTACATCTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGttttt                                                          <  1:1095818/134‑1 (MQ=255)                                                                                                        gttctttaaatgatttttttttttACATTTTTT‑TGTTTTA‑AGC‑AATAAAAAAT‑TAACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATcc                                                   <  1:203540/135‑1 (MQ=255)                                                                                                         cTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGGTATCGCTTTTAAAATCGCGTTTTTCATATcccggat                                              >  2:75007/1‑146 (MQ=255)                                                                                                          tGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGGGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGGGAAAATATTTAGAAATTAGCAAAGCAACATTAAAGGGTGGTATCGATTTTTAAAATGCGTTTTTCTGATCCTgga                                               >  2:898121/1‑147 (MQ=255)                                                                                                          tGTT‑AATG‑GATTTTATTTTTAATTTTTTT‑TGTTTTT‑GCT‑TATAGAAAAG‑TTTTTCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGt                                                <  2:464255/148‑1 (MQ=255)                                                                                                            tt‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAAACATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGt                                                >  2:1333282/1‑146 (MQ=255)                                                                                                               aaTG‑GTTTTTTTTTTTATAATTTTT‑TTTGTTT‑AGA‑CATAAAAAAA‑ACAAGCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGc                                         <  1:421664/151‑1 (MQ=255)                                                                                                               aaTG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAAACATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTTTATAAAGc                                         >  2:1212531/1‑151 (MQ=255)                                                                                                               aaTG‑AATTTTATTTTTACAATTTTT‑TGTGTTT‑AGC‑CATATAAAAA‑CCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAg                                          <  2:298879/150‑1 (MQ=255)                                                                                                                aaggaattttattttAACATTTTTT‑TTTTTTT‑GGC‑AATAAAAAAA‑TCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACa                                           <  2:1034305/144‑1 (MQ=255)                                                                                                                 tG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTTAAATATTTACAACTTAGCAATCAACCATTAACACTTGATATCGATTTTAAAGACGCGTTTTTTATATCCCGTATAAAGCt                                        >  2:863120/1‑150 (MQ=255)                                                                                                                  g‑gATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTTAAATAATTACAACTTTGCAATCCACCCTTAACGCTTTATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGtat                                              >  2:1229416/1‑143 (MQ=255)                                                                                                                       ttttttttTATAATTTTTATGTATTA‑CAG‑AATAAAAAAA‑TCATCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAc                                     <  1:1237004/149‑1 (MQ=255)                                                                                                                       ttttttttTAAAATTTTT‑TTTGTTT‑AGA‑AAAAAAAAAA‑TCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAcgc                                   <  1:588662/150‑1 (MQ=255)                                                                                                                          attttTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCAATAACGCTTGATATTGCTTTTTAAGTCGCCTTTTTTATATCCTGTATACAGCTGAcgcg                                  >  1:416917/1‑148 (MQ=255)                                                                                                                           tttttACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACATAGCAATCAACCATTAAAGCCTGATATCGCTTTTAAAGTCGCGTTTTTCATATACTGTATACAGCTGACGCGGAc                               >  1:929335/1‑150 (MQ=255)                                                                                                                           tttttACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAAATTAACAATCAACCATTAAAGCTTGATATCGCTTTTAAAATCGCGTTTTTTATATCCTGTATACAGCTGACGCGGAc                               >  1:270441/1‑150 (MQ=255)                                                                                                                           tttttACAATTTTT‑TGTGTTA‑AGG‑CATATAAAAA‑CCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGAc                               <  2:96249/150‑1 (MQ=255)                                                                                                                           tttttAAATTTTTT‑TTTGTTA‑AGG‑AAAAAAAAAA‑TAAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCgg                                 <  1:977043/148‑1 (MQ=255)                                                                                                                                aaatttttt‑ttttttt‑AGG‑AATAAAAAAA‑ACAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc                           <  1:93659/145‑1 (MQ=255)                                                                                                                                      ttt‑tGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCACTTTTTAAGACCCGTTTTTCATATTCTGTATACAGCTGACGCGGACGGGCAATCtt                     >  2:479524/1‑149 (MQ=255)                                                                                                                                              ttt‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTTAAATATTTACAACTTAGCAATCAAACATTAAAGCTTGATATCGCTTTTTAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGGAGGGCAAACTTCATACCGt             >  1:1389566/1‑150 (MQ=255)                                                                                                                                                t‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTTAAATATTTAAAAATTAGCAATCAAACCCTAACGCTTGATATCGCCTTTAAAGTCGCGTTTTTTATTTTCTGTATAAAGCTGACTCCGCCGGGCAATCTTCATTCCGtta           >  1:662563/1‑148 (MQ=255)                                                                                                                                                t‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCAc          >  2:606253/1‑151 (MQ=255)                                                                                                                                                t‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGGTAAAATATTTACAAATTAGCAATCAACCATTAAAGCTTGATATCGCTTTTAAAATCGCGTTTTTCCTATCCTGTATACAGCGGGCGCGGACGGGCAATCTTCATACCGTCAc          >  2:229885/1‑151 (MQ=255)                                                                                                                                                    g‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTTAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAATCGCGTTTTTCATATCCTTTATACAGCTGACGCGGACGGGCAATCTTTCTTCCGTCACcgt       >  1:20680/1‑151 (MQ=255)                                                                                                                                                          taAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCAtt  >  2:741274/1‑151 (MQ=255)                                                                                                                                                          |                                                                                                                                                        ATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTT‑AATG‑GATTTTATTTTTACAATTTTT‑TGTGTTT‑AGG‑CATATAAAAA‑TCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATT  >  NC_000913/752977‑753275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑