Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913862,5590GT38.7% 103.6 / 41.1 75H16Q (CAC→CAAybiWputative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base G (14/32);  new base T (29/0);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 1.76e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGCGTCAT  >  NC_000913/862416‑862709
                                                                                                                                               |                                                                                                                                                       
gTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCtt                                                                                                                                                  <  1:859927/151‑1 (MQ=255)
 tGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGAGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCTCAATAAACACCAGCGCATTTTTTTGCGCttt                                                                                                                                                 >  1:444816/1‑151 (MQ=255)
  gCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTa                                                                                                                                               <  2:239307/151‑1 (MQ=255)
     aTTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTCCAATATTCACCAGCGCCTTTTTTTGCGCcttt                                                                                                                                                >  1:280691/1‑147 (MQ=255)
        cGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCgg                                                                                                                                         <  2:481025/151‑1 (MQ=255)
        cGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCgg                                                                                                                                         <  2:1091595/151‑1 (MQ=255)
          ccAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTc                                                                                                                                       <  1:1193229/151‑1 (MQ=255)
           cAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTTCAAAATGCCCCAGCGCCATTTTTTGCGCTTTTATTCGGGCg                                                                                                                                      >  2:1052268/1‑151 (MQ=255)
            aGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTTCAATATGCACCAGCGCATTTTTTTGGGCTTTTATGCgg                                                                                                                                         >  2:947365/1‑147 (MQ=255)
            aGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAAAATTGACCCGCGCAGTTTTTTTCGCGTTTATgt                                                                                                                                           >  1:1252081/1‑144 (MQ=255)
             ggTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCt                                                                                                                                    <  2:563527/151‑1 (MQ=255)
              gTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTg                                                                                                                                   <  1:842312/151‑1 (MQ=255)
               tGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACCAAATGCACCCGCGCCTTTTTTTTCGCTTTAATTGGGGCGCTg                                                                                                                                   >  1:493682/1‑150 (MQ=255)
                gATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAg                                                                                                                                 <  1:1374803/151‑1 (MQ=255)
                  tGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAAAATGCACCAGCGCATTTTTTTGTGCTTTTAAGCGGGTGGTGAGCg                                                                                                                               >  2:368418/1‑151 (MQ=255)
                     gcCAGTGCCCGCGCGCGACGGACTGAGATTGGCTTATCGAGATGTTGTGGATACATCTCGGTATAGTGCTGCGCACGCTGGGTACAGACTGGCGGTTTCACCATGTGCGCCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCgta                                                                                                                             <  1:123448/150‑2 (MQ=255)
                     gcCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATAATCCCCCGCGCCTTTTTGTGCGCTTTTATTCGGTCGCTGAGAgtg                                                                                                                             >  2:988429/1‑150 (MQ=255)
                             cAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTc                                                                                                                    <  1:1180756/151‑1 (MQ=255)
                                cgcgcgACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAAAATGCACCAGCGCCTTTTTTTGGGCTTTTATGCGGGCGCTGGGCGGGTCCAGttt                                                                                                                     >  2:1144110/1‑147 (MQ=255)
                                      aCGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATATGCACCAGCGCATTTTTTTGCGCTTTTATTCGGGCGCTGGGCGTGTCCCGTTTCAGTGTgg                                                                                                             >  2:1259879/1‑149 (MQ=255)
                                      aCGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATATGCACCAGCGCATTTTTTTGCGCTTTTATGCGGGCGCCGAGCGTGTGCAGGTTCAGGGTgg                                                                                                             >  1:745756/1‑149 (MQ=255)
                                        gTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATATGCACCAGCGCACTTTTTTGCGCTTTAATTCGGTCGCTGGGCGGGTCCCGTTTCCGTGTGGTCAt                                                                                                         >  2:964922/1‑151 (MQ=255)
                                          aCCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATg                                                                                                        <  1:1033453/150‑1 (MQ=255)
                                           ccGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACCATATGCACCAGCGCAATTTTTTGGGCTTTAATATGGTGGCCGAGCGTGTTAAGGTTCAGTGCGGTCCTgg                                                                                                       >  2:714538/1‑150 (MQ=255)
                                           ccGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACCAAATGCACCCGCGCCATTTTTTGCGCTTTTATGCGGGGGCCGAGGGTGGGCCGCTTTAGTGTgggcgttg                                                                                                       >  1:288496/1‑144 (MQ=255)
                                           ccGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATATGCACCAGCGCATTTTTTTGGGCTTTAATGCGGGCGCTGGGCGGGTCCAGTTTCCGTGTgggcct                                                                                                         >  2:1407727/1‑144 (MQ=255)
                                                aTCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAAAAAACAACAGAGGAATTTTTTTTGCTTTAAATCGGGGGCTGAGCGGGTCCCCTTTTAATGTGGGCATGggtgtt                                                                                                  >  1:1019014/1‑150 (MQ=255)
                                                   ggCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGGTTTAAAATGTACACCAGCGCATTTTTGTGCGCGTTAATGTGGGCGCTGAGCGGGTGCAGGTTCAGTGGGGTCGTGgttgtt                                                                                                  >  2:868730/1‑147 (MQ=255)
                                                    gCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCt                                                                                             <  1:54429/151‑1 (MQ=255)
                                                    gCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGCTTCACAATTTTCACCACTGCATTTTTTGGGGCTTTAAATTCGGCGCCGAGCGCGTCCACATTTAGGGTgggtccgggt                                                                                                     >  2:1192457/1‑135 (MQ=255)
                                                       tATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCg                                                                                           <  2:926180/150‑1 (MQ=255)
                                                           gagaTGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCCCAATATTCACCAGTGCCGTTTTTTGCGGCTTTATGCGGGCGGTTGGCGTGTTCCCGTTCACGGGGGTCATGGTTGTTTTTCTTGTac                                                                                        >  1:668332/1‑148 (MQ=255)
                                                           gagaTGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGGCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTaa                                                                                        <  1:512622/149‑1 (MQ=255)
                                                            agaTGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGt                                                                                     <  2:99737/151‑1 (MQ=255)
                                                             gaTGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTc                                                                                    <  2:1113177/151‑1 (MQ=255)
                                                              aTGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGGTTCTCAAAATGCACCCGCGCCTTTTTTTGGGCTTTTATGCGGGCGCCGAGCGTGTCCCGGTTCTGTGTGGGCATGGTTGTTTTCCTCGTAAGGGcg                                                                                   >  1:1114493/1‑151 (MQ=255)
                                                               tgttgtTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCCTTTTTTTGCGCTTTTATGCGGTCGCTGAGCGTGTCCCGTTTTTGTGTGGGCATGGTTGTTATCCTCGTAAgg                                                                                      >  2:957235/1‑147 (MQ=255)
                                                                 ttgttgATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAAAATGCACCCACGCATTTTTTTGCGCTTTAATGGGGGCGCTGAGCGTGTCCAGTTTTAGTGTGGGCATGGTTGTTATCCTCGTAAGGTCGCgg                                                                                >  2:87547/1‑151 (MQ=255)
                                                                  tgttgATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCgg                                                                                <  1:659789/150‑1 (MQ=255)
                                                                   gttgATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATATTTCCCAGCCCCTTTTTTTTGGCTTTTATGCAGGCGCCGGGAGGGGCCCGTTTCAGTGGGGGCCTGGTGGTTTTTCTCCTAAAGTc                                                                                    >  1:1365107/1‑145 (MQ=255)
                                                                   gttgATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGtt                                                                              <  2:1108958/151‑1 (MQ=255)
                                                                       aTACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGGTTCAAAATATGCAACAGCGCATTTTTTTGTGCTTTTATTGGGGGGCTTCGGGTGTGCAGTCTTAGTGGGGGGATGGTTGGTATTCTCGCAAGGTGGCgggt                                                                              >  1:1271419/1‑145 (MQ=255)
                                                                          cATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCTCAATAAGACCCCGCGCACTTTTGTGGGCTTTAATGCGGGGCCTGAGCGTGTCCAGATTTAGTGGGGGTCTGGGTGGTTTCCTCGTCAGGGCGCGGGTAACCCtt                                                                       >  1:48605/1‑151 (MQ=255)
                                                                          cATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCtt                                                                       <  2:1191014/151‑1 (MQ=255)
                                                                             ctcGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTCCAATATGCACCAGCGCATTTTTTTGCGCTTTTATGCGGGCGCTGAGCGTGTCCCGTTTTTGTGTGGGCATTGTTGTTATCCTCGTAAGGGCGCGGGTTACCCTTTCt                                                                    >  2:1047203/1‑151 (MQ=255)
                                                                              tcGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGGTTCTCAATATGCACCCACGCCTTTTTTTTGGCTTTTTTGCCGGCGCTGCGCGGGTCCCGTGTCCCGGTGGTGTCGGGTTTTTTCCCTGTAAGGGCGCGGGGTACCCttt                                                                      >  2:1190775/1‑148 (MQ=255)
                                                                               cGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCt                                                                    <  1:1112861/149‑1 (MQ=255)
                                                                                      gTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATATGCACCAACGCATTTTTTTGCGGCTTTATGTGGGCGGGGAGCGTGTCCCAGTTCCGTGTGGGCATGGTTGTTATCCTCTTGAGGGCGCGGTTAATCCTCTCTtgcgggcac                                                           >  1:243141/1‑150 (MQ=255)
                                                                                       tgctgcGCGCGCTCGGTACAGACTGGCGGTTTTACAATATGCCCCAGCGCCTTTTTTTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTTAGTGTGGGCATGGTTGTTATCCTCGTTAAGTGGCGGGTTACCCTTTTTGGCAGGCAt                                                           >  2:760682/1‑150 (MQ=255)
                                                                                          tgcGCGCGCTCGGTACAGACTGGCGGTTTCCCAAAATGCACCCGCGCATTTTTGTGCGCTTTTATGCGGGCGCTGCGCGTGGCCAGGTTTAGTGTGGGCCTGGGTGTTTTTCTCGTAAGGTCGCGGGTAAACCCTTCTTGCAGGGATAct                                                        >  1:1008440/1‑150 (MQ=255)
                                                                                           gcgcgcgcTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATActg                                                       <  1:166150/150‑1 (MQ=255)
                                                                                           gcgcgcgcTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATActg                                                       <  2:1089032/150‑1 (MQ=255)
                                                                                           gcgcgcgcTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCACGCATActg                                                       <  2:1000786/150‑1 (MQ=255)
                                                                                            cgcgcgcTCGGTACAGACTGGCGGTTTCACTATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATActgct                                                     <  2:782392/151‑1 (MQ=255)
                                                                                                cgcTCGGTACAGACTGGCGGTTTCACCAAATTCAACACGGCCCTTTTTTGCGCCTTCAAATGGGGGGTGTGCGAGTGCAGGTTTTGTGTGGGCCTGGTTGTTTTTCTCCGAAGGGCGCGGGTAACCCCTTCTGGGAGGCATActcattg                                                   >  1:1414597/1‑144 (MQ=255)
                                                                                                  cTCGGTACAGACTGGCGGTTTTACAAAATGCACCCACGCATTTTTTTTCGCTTTAATGGGGTCGCTGAGCGTGTCCCGTTTCAGTGTGGTCCTGGTTGTTTTTCTCGTAAAGGCGCGGGTAAACCCTTTTTGCAGGCATACTGCTggg                                                  >  1:767338/1‑148 (MQ=255)
                                                                                                   tCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTTTGCGCTTTAATGAGGTCGCTGAGCGGGTCCAGTTTCAGTGGGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGGaa                                               >  2:1123990/1‑150 (MQ=255)
                                                                                                    cGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCaaa                                              <  1:643548/150‑1 (MQ=255)
                                                                                                              tGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGtt                                   >  2:269102/1‑151 (MQ=255)
                                                                                                               ggCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTc                                  >  2:1347498/1‑151 (MQ=255)
                                                                                                                 cGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTg                                <  2:41901/151‑1 (MQ=255)
                                                                                                                     ttCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGgcgc                            >  1:138293/1‑151 (MQ=255)
                                                                                                                        acaATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATc                         <  1:1255710/151‑1 (MQ=255)
                                                                                                                         caATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATc                         >  1:119152/1‑150 (MQ=255)
                                                                                                                          aaTATGCACCAGCGCTTTTTTGTGCGCTTTAATGCGGTCGCTGAGCGCGTCCAGTTTCTGTGTGGTCGTGGTTGTTATCCTCGCTAGGTCTCGGTTAACCCTTTCTGGCAGGCTTACTGTTGCGCAAAGTCGAGCAGGTCTGgcgc                            >  1:478503/1‑146 (MQ=255)
                                                                                                                          aaTATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCaa                       <  2:1264099/151‑1 (MQ=255)
                                                                                                                           atatGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAg                      <  1:427402/151‑1 (MQ=255)
                                                                                                                            tatGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGc                     >  2:359277/1‑151 (MQ=255)
                                                                                                                                  ccAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGtttt               <  2:1305892/151‑1 (MQ=255)
                                                                                                                                   cAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGttttt              >  2:1108779/1‑151 (MQ=255)
                                                                                                                                     gcgcATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTcc            >  1:1388100/1‑151 (MQ=255)
                                                                                                                                         aTTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCgggg        >  2:21848/1‑151 (MQ=255)
                                                                                                                                          tttttGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGc       >  1:338059/1‑151 (MQ=255)
                                                                                                                                           ttttGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGc       <  2:340088/150‑1 (MQ=255)
                                                                                                                                            tttGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCATTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGCg      <  2:584397/150‑1 (MQ=255)
                                                                                                                                               gtgCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGCGTCAt  <  2:555993/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                       
GTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTTAATGCGGTCGCTGAGCGTGTCCAGTTTCAGTGTGGTCATGGTTGTTATCCTCGTAAGGTCGCGGTTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAGCGGTTTTTCCGGGGCGTCAT  >  NC_000913/862416‑862709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: