Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,080,8560TG43.4% 76.7 / 12.1 53intergenic (+43/‑500)putP/efeUproline:sodium symporter/ferrous iron permease (pseudogene)
Reads supporting (aligned to +/- strand):  ref base T (9/21);  new base G (23/0);  total (32/21)
Fisher's exact test for biased strand distribution p-value = 5.73e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATG  >  NC_000913/1080709‑1081003
                                                                                                                                                     |                                                                                                                                                    
tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCCCTATCATTCGGGTCCGCCGTCACGGTTGCAGGAAAGCTAAGTGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGtt                                                                                                                                                     <  1:982381/149‑1 (MQ=255)
    gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTTGATTTAGCAGCAGGGTTggccc                                                                                                                                                >  2:367959/1‑147 (MQ=255)
     tttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGTTCAGCAGCGGGGTGCGCTcc                                                                                                                                             >  2:902783/1‑151 (MQ=255)
       tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCCGCAGCGGGTTGGGCTcccc                                                                                                                                           >  2:1104824/1‑150 (MQ=255)
        gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTTAGCAGCGGGGTGCGCccccct                                                                                                                                          >  2:353858/1‑149 (MQ=255)
              tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGCTTTGTTTGTCCTCTCCAGCGGGGTGCGCCCCCtt                                                                                                                                          >  1:43988/1‑145 (MQ=255)
                aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGTTCAGCAGCGGGGTGCGCTCCCCTAAtgtg                                                                                                                                    >  2:74720/1‑149 (MQ=255)
                aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAGCGGGTTGCGCCCCCTTAATGTGcc                                                                                                                                  >  2:695147/1‑151 (MQ=255)
                aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGcc                                                                                                                                  <  2:499386/151‑1 (MQ=255)
                    gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTc                                                                                                                                <  1:1327321/149‑1 (MQ=255)
                     ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTTTTTTGCTTCTGCCGCGGGGTGGGCCCCCCTTAATTGGCTCGcc                                                                                                                             >  1:989537/1‑151 (MQ=255)
                      cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGCTTTTTTTTTCTTCCGCAGCGGGGTGGGCTCCCTTTAAATGCCCCGccc                                                                                                                            >  1:404643/1‑150 (MQ=255)
                       gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGGTGCGCCCCCCTTATTTGCCTcccc                                                                                                                             >  2:978747/1‑149 (MQ=255)
                        tCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTTTTTTGCTTTCGCAGCGGGGTTCGCCCCCCTTATGTGcc                                                                                                                                  >  1:682476/1‑143 (MQ=255)
                         cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCata                                                                                                                          <  1:33134/150‑1 (MQ=255)
                           gcggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATaa                                                                                                                       <  2:779258/151‑1 (MQ=255)
                              gcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAAtt                                                                                                                    <  2:475270/151‑1 (MQ=255)
                                gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGa                                                                                                                  <  1:1402504/151‑1 (MQ=255)
                                 aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGTTCCGCAACGGGGTTCGCCCCCTTAATGGGCCTCGCCCAATaaaat                                                                                                                    >  2:517254/1‑146 (MQ=255)
                                 aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGGGTCTACAGAGGGGTGGGCGCCCCTAAAGTGGCCTGCCCTATaaa                                                                                                                      >  1:1366367/1‑146 (MQ=255)
                                  tGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCAGCAGCGGGGTGCGCCCCCTTAATGTGCCTCGCCCTATAAATTTaa                                                                                                                 >  2:12559/1‑150 (MQ=255)
                                    cAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATgg                                                                                                              <  2:671670/151‑1 (MQ=255)
                                    cAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATgg                                                                                                              <  1:1383551/151‑1 (MQ=255)
                                    cAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGCTTTTTTTGGTGTCAGCAGGGGGGAGCGCTCCCCTTAAATGTCCCTCCACATAAATTTaa                                                                                                                 >  1:756837/1‑148 (MQ=255)
                                                      gCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGGCTCAGCAGCGGGGTTCGCCCCCTTTATTTTGCCCGCCCTATTAAATGGATGGGGCAGGGGGCGCGCCggg                                                                                            >  1:850239/1‑151 (MQ=255)
                                                         gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCAGCAGCGGGGTGCGCTCCCTTAATGTGCCTCGCCCTATAAATTGAATGGTGCAGGGAGCGCGCAggggg                                                                                          >  2:147712/1‑150 (MQ=255)
                                                         gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGGTTCAGCAGCGGGGTGCGCCCCCTTTATGTGGCTCGCCATATAAATTGAAAGGTGCAGGGGGGGCGCAggggg                                                                                          >  1:762179/1‑150 (MQ=255)
                                                         gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGggc                                                                                         <  2:1302452/151‑1 (MQ=255)
                                                          aTGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAGCGGGGTGCGCTCCCTTTATGTGCCCCCCCCTATAAATTTAATGGGGCAGGGGGCGCGCAGGGggcg                                                                                        >  2:932369/1‑151 (MQ=255)
                                                          aTGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGggc                                                                                         <  1:451461/150‑1 (MQ=255)
                                                              gcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTTGCTTCATCAGCGGGGTGCGGCCCCTTAATGTGTCCCGCCATATTAAATGAAATGTGCAGGGAGCGCGCAGCgggggg                                                                                       >  2:502132/1‑148 (MQ=255)
                                                                aCTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTTGGTTTACCCGCGGGGTGCGCCCCCTTAATTAGGCTCCCCCCATTAATTTAATGGTGGAggg                                                                                                       >  1:1325471/1‑130 (MQ=255)
                                                                     cATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATcgcc                                                                              <  2:287713/150‑1 (MQ=255)
                                                                         cGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGTTCAACAGCGGGGTGCGCTCCCTTAATGTGCCTCGCCCTATAAAATGGATGGTGCAGGGAGCGCGCAGGGGGCGGCCAAAcgccccc                                                                           >  2:533477/1‑149 (MQ=255)
                                                                         cGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGGTTCAGCACGGGGGTGGGCGCCCCTGATGGGGCTCCCCCTATTAATTGAATGATGCCGGGAGCGCGGAGGGGGCGGCCAACcgcggccg                                                                          >  1:278491/1‑150 (MQ=255)
                                                                         cGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGAGAGGGCGCAGGGGGCGGCCAATcgccgccg                                                                          <  2:180379/150‑1 (MQ=255)
                                                                               cgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAGCGGGTTGCGCCCCCTTAATGTGCCTCGCCCTATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGGGGCCCATCGCCGCCGccccc                                                                     >  2:579799/1‑149 (MQ=255)
                                                                                 ccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCctgct                                                                 <  1:991564/151‑1 (MQ=255)
                                                                                  cgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGCTTTTTTTTGGTTTAGCAGCGGGGTGGGCTCCCTTTAACTGCCCCGCCCCATAAAATGAAAGGTGGCGGGGGCGGGCAGGGGGCGGCCCAACGCCGCCGccccc                                                                     >  2:604061/1‑146 (MQ=255)
                                                                                      aCGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTccc                                                            <  1:365369/151‑1 (MQ=255)
                                                                                             cAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGGTGCGCTCCCCTTATGTGCCTCGCCCTATAAATTGAATGGTGCAAGGGGGGCGCCGGGGGGGGGCAATCGCCGCCGCCCCCTGCCGGCCCCGcc                                                        >  2:1076577/1‑148 (MQ=255)
                                                                                                gAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCggg                                                  <  2:717629/151‑1 (MQ=255)
                                                                                                    gCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCACCCGGGTTCCGTCCCTTAATGTGCCTCGCCCTATAAAATGAATTGTGCAGGGGGAGCGCAGGGGGGGGCCAATCGCCGCCGCCCCCCGCTGTCCCCGCCTTCgggg                                                 >  2:618952/1‑148 (MQ=255)
                                                                                                        aGGGACTTAGCCTGCGGCGGTTTTGTTTTGGTTTAGCAGCGGGGTGCGCTCCCCTAATTTGGCTCGCCATATAAATTGAATGGGGCCGGGGGGGCGCCAGGGGGGGCCAAACGCCCCCGCCCCCCGCCGTTCCGGCCCTCgggg                                                 >  1:1221169/1‑144 (MQ=255)
                                                                                                           gACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAg                                       <  1:596537/151‑1 (MQ=255)
                                                                                                                   cTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGAtttt                                <  2:228118/150‑1 (MQ=255)
                                                                                                                          ggTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccac                        >  1:24758/1‑151 (MQ=255)
                                                                                                                             tttgtttgGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca                      >  1:797258/1‑150 (MQ=255)
                                                                                                                                  ttgGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACAccc                >  2:766162/1‑151 (MQ=255)
                                                                                                                                   tgGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCg               >  1:161931/1‑151 (MQ=255)
                                                                                                                                            cagcGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGttat      <  2:623410/151‑1 (MQ=255)
                                                                                                                                               gcGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGttatta    <  2:198121/151‑1 (MQ=255)
                                                                                                                                                cGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTtattat   <  2:1119810/151‑1 (MQ=255)
                                                                                                                                                  gggTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATg  <  1:367946/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACACCCGAGCTGTTATTATG  >  NC_000913/1080709‑1081003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: