Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,483,2680TA41.8% 95.7 / 47.2 79L24F (TTA→TTTemrKmultidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (27/19);  new base A (0/33);  total (27/52)
Fisher's exact test for biased strand distribution p-value = 4.67e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGC  >  NC_000913/2483118‑2483417
                                                                                                                                                       |                                                                                                                                                      
gTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat                                                                                                                                                        >  1:922653/1‑151 (MQ=255)
  tCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaata                                                                                                                                                       >  2:103380/1‑150 (MQ=255)
  tCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaata                                                                                                                                                       <  2:137755/150‑1 (MQ=255)
   cGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAataaataa                                                                                                                                                      >  1:502002/1‑150 (MQ=255)
          ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACt                                                                                                                                                 <  2:320334/147‑1 (MQ=255)
          ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTa                                                                                                                                                <  1:805653/148‑1 (MQ=255)
           tATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCg                                                                                                                                             <  1:383288/150‑1 (MQ=255)
           tATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGc                                                                                                                                            >  2:958042/1‑151 (MQ=255)
              gATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGCCCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCaa                                                                                                                                         <  1:638872/151‑1 (MQ=255)
              gATTAACGACAGTGACACTACCGGAGACTTGTGCAGAAATTGAATCTGCATTCCCCGTTACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAACCGCAATAAATAAATCTACCGCCa                                                                                                                                          <  1:372483/150‑1 (MQ=255)
               aTTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCaa                                                                                                                                         <  2:1264705/150‑1 (MQ=255)
               aTTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt                                                                                                                                        >  1:1317537/1‑151 (MQ=255)
                  aaCGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATaa                                                                                                                                      >  1:1414004/1‑150 (MQ=255)
                        aGTGACACTCCCTGAGACTTGTGAAAAAATTGGATCTCCATTCCCCGTGACATAGGCGTCATCTGTACAAATATTGTCTTCAAATCCCATTAACAAAAAGGAAAAGGCACCTAAAAACGCAATAAAAAAACCTACCGCCAATAAAGaaaa                                                                                                                                <  1:645387/150‑1 (MQ=255)
                          tGACACTACCTGAGACTTGTGCAAAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATTTCTTCAAATTCCATTGACCAATAGGCATAGGCCCCTGAAACCGCAATAAATAAAACTACCGCCAATAAAGAAAAg                                                                                                                               <  2:359422/149‑1 (MQ=255)
                           gACACTACCTGAGACTTGTGCAGAAATTGGATCTGAATTCCCCGCGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACAAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGACAATAAAGAAAAGGAt                                                                                                                            >  1:1118815/1‑151 (MQ=255)
                                  ccggagaCTTGTACAGAAATTGGTTCTGAATTCCCCGTAAAAAAGGCTTCATCTGTACTAATAATTTCTTCTAATACCATTAACAAAAAGGAAAAGACACCTAAAACCCAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTttc                                                                                                                        <  1:148462/145‑1 (MQ=255)
                                  ccTGAGCCTTGTGCAGAAATTGGATCTGCATTCCCCGTAACATAGGCGTCATCTGTACAAATCATGTTTTTTAATCCCATTGACCAATAGCCATGGGCCCCAGAAAACGCAAAAAAAAAACCTACCGCCAATAAAGAAAAGTATTttcttc                                                                                                                     <  2:245782/151‑1 (MQ=255)
                                  ccTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttcttc                                                                                                                     >  2:655364/1‑151 (MQ=255)
                                     gagaTTTGTGAAAAAATGGGATCTGCATCCCCCTTGACATAGGCTTCATTTGTACAAATCATTTTTTAAAATCCCATTAACAAATAGGAATAGGCCCTTAAAAACCAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTtcttct                                                                                                                    <  1:932929/149‑1 (MQ=255)
                                     gagaCTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAACCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTtcttct                                                                                                                    <  2:1259449/149‑1 (MQ=255)
                                     gagaCTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGt                                                                                                                  >  1:298012/1‑151 (MQ=255)
                                       gttttGTGCAAAAATTGGTTCTGCATTCCCCGTGACAGAGGCGTCATCTGTACTAATCATTTTTACTAATTCCATTGCCCAATAGGAAAAGGCACCTGAAACCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGt                                                                                                                  <  1:962778/146‑1 (MQ=255)
                                       gttttGTGAAAAAATTGGATCTGAATCCCCCGTGAAATAGGCGTCATCTGAACAAATAATTTCTTAAAATTCCATGGACAAAAAGGAATAGGCACCTAAAACCCAAAAAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGtt                                                                                                                 <  1:913966/147‑1 (MQ=255)
                                       gttttGTGAAAAAATTGGATCGGCTTCCCCCGTGAAATGGGCTTCATCTTTACTAAACATGTCTACAAATTCCATTGACCAAAAGGAATAGCCACATAAAAACGCAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTtcttct                                                                                                                    <  1:821735/144‑1 (MQ=255)
                                          ttGTGCAAAATTTGGATCTCCATCCCCCGTGAAATGGGCTTCTTCTGTACTAATCATGTCTTCTAATTCCATTACCAAAAAGAAATAGGCACCTAAAAACAAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg                                                                                                               <  1:1389627/149‑1 (MQ=255)
                                           tgtgAAAAAATTGGATCTGCATTCCCCGTGACATAGGCTTCATCTGTACTAATCATGTCTTCTAATTCCATTGCCCAATGGGCATGGGCACCTGAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa                                                                                                             <  2:114095/150‑1 (MQ=255)
                                            gtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATg                                                                                                           >  1:528014/1‑151 (MQ=255)
                                            gggCAAAAATTGGATCTGCATTCCCCGTAAAATGGGCGTCATCTGTACAAATCTTGTCTTCTAATCCAATAGACAAAAAGGCAAAGGCACCTAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATg                                                                                                           <  2:836338/149‑1 (MQ=255)
                                                aGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttt                                                                                                       >  1:550055/1‑151 (MQ=255)
                                                  aaaTTGGATCTGCTTTCCCCGTGACATAGGCGTCATCTGTACTAATCATTTCTTCTAATTCCATTGACCAATAGGCATAGGCCCCTAAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttttt                                                                                                     <  2:1162077/151‑1 (MQ=255)
                                                  aaaTTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTTTTCTAATTCCATTGACAAAAAGAAATAGGCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttttt                                                                                                      <  1:429324/150‑1 (MQ=255)
                                                  aaaTTGGATCTGCATTCCCCGGGACATGGGCGTCATCTGTACTAATCATGTCTTCTAATCCAATTGACAAATGGGCATAGCCACCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttttt                                                                                                     <  2:1051752/151‑1 (MQ=255)
                                                  aaaTTGGATCTGCATCCCCGGTGAAATAGGCGCCATTTGTACTAATCATTTCTTTAAATTCCATTGACAAATAGGCATAGGCCCCTAAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttttt                                                                                                     <  2:1051366/151‑1 (MQ=255)
                                                  aaaTTGGATCGGCTTTCCCCGTGACATAGGCGTCTTCTGTACTAATCTTGTCTTTTAATTCCATTGCCCAATAGGAATAGCCCCCTGAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttttt                                                                                                     <  2:1162242/151‑1 (MQ=255)
                                                       gaaTCTGCATCCCCCGTGACATGGGCGTCATTTGAACTAATCATTTCTTCTAATCCCTTTCACCAAGAGGCATAGGCACCGGAAACCGCAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAtt                                                                                                  <  2:1100285/147‑1 (MQ=255)
                                                          tccgccttCCCCGGGACAGAGGCGTCATCTGTTCAAATCATTTTTTCTAATCCCATTGACAAATAGGAATAGCCACATCAAAACGTAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGaa                                                                                              <  2:464793/144‑1 (MQ=255)
                                                            ttcttaccccGCGTCATAGGCTTCATTTCTGATAATCATTTCTTCTAATTCATTTGTCCACACGCCAGCGGCCCCTGAAAACGAAAAAAAAAAAAATACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGaa                                                                                              <  2:764556/142‑1 (MQ=255)
                                                             gCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa                                                                                           >  2:157917/1‑150 (MQ=255)
                                                                ttCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt                                                                                       >  1:714822/1‑151 (MQ=255)
                                                                 tCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTg                                                                                      >  2:64183/1‑151 (MQ=255)
                                                                  ccccTTGACATAGGCGTCATCTTTCATAATCATGTTTTATAATCCCATTACCAAAAAGGAAAAGGCCCCTGAAACCGAAAAAAAAAAAATTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt                                                                                       <  1:1004452/149‑1 (MQ=255)
                                                                    ctttGACAGAGCCGTTATCTGTAAAAATCATTAATTCTATTCTCATTGCCCAATAGGCACAGGCAAAAAAAAACGAATTAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt                                                                                       <  2:1000337/144‑1 (MQ=255)
                                                                      gTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCGGAAAGCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGATAGAATGTTTTTTATTTGAATTAATCTGTTcc                                                                                  >  1:564739/1‑150 (MQ=255)
                                                                       tGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCAc                                                                                >  2:275050/1‑151 (MQ=255)
                                                                        gACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt                                                                               >  2:840220/1‑151 (MQ=255)
                                                                           aTAGGCTTTATCTGAACAAATCATATCTTTAAATTCCATTACCCAAGAGTGATAGCCCCTTAAAAGCTAAAAAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatt                                                                            <  1:603406/151‑1 (MQ=255)
                                                                           aTAGGCGTCATCTGTACTAATCATGTTTTCAAATTTCATTGACAAATAGGCATAGGCCCCTAAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtat                                                                             <  2:891641/150‑1 (MQ=255)
                                                                              ggCTTCTTCTTTACTATTAATGTTTTATAATTCCATTAACAAATAGGCATAGCCACGGAAAAACGAAAAAAATAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatta                                                                           <  1:1240516/149‑1 (MQ=255)
                                                                              ggCGTCATTTTTACAAATCATGTTTTCAAATCCCATTGACCAATAGACATAGCCACCTAAAAACGCAATAAAAAAATTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtattat                                                                          <  1:59881/150‑1 (MQ=255)
                                                                               gCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtct                                                                        >  1:1088816/1‑151 (MQ=255)
                                                                                cGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctc                                                                       >  2:950683/1‑151 (MQ=255)
                                                                                 ttCATCTTTCCAAATAATGTTTTAAAATTCCATTGACAAAAAGGAATAGGCACTTAAAAACAAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtattat                                                                          <  1:1130588/146‑1 (MQ=255)
                                                                                 gTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACTCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctct                                                                      >  2:364408/1‑151 (MQ=255)
                                                                                   cATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGATTTTTATTTGAATTAATCTGTTCCACTATTAtctctc                                                                     >  2:941051/1‑150 (MQ=255)
                                                                                       tGTACTAATCATGTCTTCTAATCCCATTGACCAATGGGCATAGGCCCCTAAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAttt                                                                 <  2:1193172/150‑1 (MQ=255)
                                                                                          acaaaTCTTGTCTTTTAATACCATTGACCAATAGGCTTAGCCACGTAAAACCACAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc                                                                <  2:159135/145‑1 (MQ=255)
                                                                                          aCTAATCATGTCTTAAATTCAAATTGCCCAATAGGCAAAGGCCCCTGAAAACCAAATAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtct                                                               <  2:611211/149‑1 (MQ=255)
                                                                                             aaTCATTTTTTCTAATTCCATTGACCAATAGGCATAGGCCCCTAAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                            <  2:298024/149‑1 (MQ=255)
                                                                                                  tGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa                                                      >  1:869660/1‑150 (MQ=255)
                                                                                                   gTCTTCAAATTCCATTGACAAATAGGCATAGGCACCTGAAAACGCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATAAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa                                                      <  2:441000/149‑1 (MQ=255)
                                                                                                    tcttctAATCCCATTGACCAATAGGCATAGGCACCTAAAAACGCAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGaa                                                     <  2:215641/149‑1 (MQ=255)
                                                                                                      ttcaAATTCCATTGACCATTAGGCAAAGGCACCTGAAACCGAAATAAATAAATCTACCGCCAATAAAGAAAATTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa                                                      <  1:1269686/146‑1 (MQ=255)
                                                                                                       tcaaaTCCCATTGACCAATAGGCATAGGCCCCAAAAAACGAAATAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAttt                                                 <  2:1187215/147‑1 (MQ=255)
                                                                                                          aaTTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtg                                              >  2:546704/1‑150 (MQ=255)
                                                                                                          aaTTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtg                                              >  1:319417/1‑150 (MQ=255)
                                                                                                          aaTACCATTGACCAATAGACATAGGCACTTAAAACCGCAATAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAt                                               <  1:427741/149‑1 (MQ=255)
                                                                                                                  ttGACAAATAGGAAAAGACACCTGAAACCAAAAAAAAAAAATTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                                         <  1:593674/148‑1 (MQ=255)
                                                                                                                   tGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatg                                     >  2:364649/1‑151 (MQ=255)
                                                                                                                        aaacgacatagccaCCTAAAATCAAAAAAAAAAAAATTACCGCCAATAAATAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATCAAATTTATGAATtgaatg                                     <  1:600607/140‑1 (MQ=255)
                                                                                                                         ataggcataggcACCTAAAACCGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAaga                               <  2:836539/151‑1 (MQ=255)
                                                                                                                           aggcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGc                             >  1:1327115/1‑151 (MQ=255)
                                                                                                                           aggaatagccaCTTAAAAACTCAAAAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAagag                              <  1:957992/150‑1 (MQ=255)
                                                                                                                             gcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcat                           >  1:231387/1‑151 (MQ=255)
                                                                                                                              cataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcatt                          >  1:1309629/1‑151 (MQ=255)
                                                                                                                               ataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattc                         >  2:376993/1‑151 (MQ=255)
                                                                                                                               aaaggcgcgtaaaacCGTAAAAAAAAAAACTGCCGCCAATGAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATGTGTTGCACTAGTATCTCTCATTTCTCATATATGAAATTTATGAATTGAATGAAAAGAGc                             <  1:681784/136‑1 (MQ=255)
                                                                                                                                  gacaCCTAAAAACACAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattca                        <  1:658617/147‑1 (MQ=255)
                                                                                                                                    caCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttct                    >  1:87569/1‑151 (MQ=255)
                                                                                                                                      ccaaaaaaCCCAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc                  <  2:143127/147‑1 (MQ=255)
                                                                                                                                      cataaaaaCAAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattc                         <  1:121240/140‑1 (MQ=255)
                                                                                                                                          aaaaCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCa                 <  2:174862/148‑1 (MQ=255)
                                                                                                                                          aaaaCGAAAAAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat                <  2:1207371/149‑1 (MQ=255)
                                                                                                                                                  ataaataaAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac       >  1:770286/1‑150 (MQ=255)
                                                                                                                                                  ataaataaAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAAcaca      <  2:1378177/151‑1 (MQ=255)
                                                                                                                                                    aaataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGt    <  2:1414097/151‑1 (MQ=255)
                                                                                                                                                    aaaaaaaaCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGt    <  2:350261/151‑1 (MQ=255)
                                                                                                                                                      ataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGc  >  1:605208/1‑151 (MQ=255)
                                                                                                                                                       taaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGc  >  2:1192319/1‑150 (MQ=255)
                                                                                                                                                       taaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGc  >  1:410476/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
GTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGC  >  NC_000913/2483118‑2483417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: