Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,404,2150TG39.8% 174.2 / 28.8 108G244G (GGT→GGG) acuIputative acryloyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (17/48);  new base G (43/0);  total (60/48)
Fisher's exact test for biased strand distribution p-value = 1.51e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCG  >  NC_000913/3404072‑3404365
                                                                                                                                                |                                                                                                                                                        
ccTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGGCTGTGGTCTGGC‑GGGGGGgttt                                                                                                                                                    >  1:1051021/1‑150 (MQ=255)
ccTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑CGGGGGattt                                                                                                                                                    >  2:115671/1‑150 (MQ=255)
ccTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCGGTGGTGTGGG‑GGGTGGgttt                                                                                                                                                    >  1:1395881/1‑150 (MQ=255)
 cTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGgttt                                                                                                                                                    >  2:792067/1‑149 (MQ=255)
 cTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGgttt                                                                                                                                                    >  1:1190129/1‑149 (MQ=255)
   cGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGTGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTAc                                                                                                                                                  <  1:1379330/149‑1 (MQ=255)
    gTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTActct                                                                                                                                               <  1:1324333/151‑1 (MQ=255)
    gTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGAGGGCTGGC‑GGAGGGTGTTAct                                                                                                                                                 >  1:273573/1‑149 (MQ=255)
      gatgaGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGtttt                                                                                                                                                    >  1:418005/1‑144 (MQ=255)
        tgaGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGcc                                                                                                                                            >  1:505246/1‑150 (MQ=255)
          aGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAAc                                                                                                                                         <  1:364331/151‑1 (MQ=255)
           gTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCCAcc                                                                                                                                        >  1:1222860/1‑151 (MQ=255)
              tGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCAc                                                                                                                                      <  2:331157/150‑1 (MQ=255)
              tGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCAc                                                                                                                                      <  2:318371/150‑1 (MQ=255)
               gCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACg                                                                                                                                     <  2:1211895/150‑1 (MQ=255)
               gCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAACCACgg                                                                                                                                    >  2:402516/1‑151 (MQ=255)
                ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCAc                                                                                                                                      <  1:1230354/148‑1 (MQ=255)
                 cGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGGGGCGGCCTGTGGTCTTGC‑GGGGGGGTTTAACCTGccacccac                                                                                                                                      >  1:129720/1‑147 (MQ=255)
                   aaTCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAACCCCGGTCa                                                                                                                                 >  1:618509/1‑150 (MQ=255)
                     tCCCGTCCTCTGGAAAACCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTTCGTGGCGGTCCTTTGAGTGGC‑GGGGGGGTTTTCCCTTCTAAACACgg                                                                                                                                    >  1:276576/1‑145 (MQ=255)
                      cccGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGc                                                                                                                              <  2:772554/150‑1 (MQ=255)
                       ccGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGTTCATGCCa                                                                                                                            <  1:1286248/151‑1 (MQ=255)
                       ccGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAACCACGGGCATGcc                                                                                                                             >  2:1176631/1‑150 (MQ=255)
                           ccTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCAttt                                                                                                                         <  2:154110/150‑1 (MQ=255)
                           ccTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTa                                                                                                                        <  2:1380350/151‑1 (MQ=255)
                           ccTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGGGGCGGCCTGGGGGGTGGC‑GGGGGGGTTTTCCCTTCCAACCACGGTCGTGcccgt                                                                                                                          >  1:663953/1‑146 (MQ=255)
                            ctctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCCACCACGGGCATGCCATTTAt                                                                                                                       >  2:1011889/1‑151 (MQ=255)
                            ctctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCATGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTa                                                                                                                        <  1:267854/150‑1 (MQ=255)
                              ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTAt                                                                                                                       <  2:934926/149‑1 (MQ=255)
                                ggAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTTCTCTGCCAACCCCGGTCATGCCATTTATTCt                                                                                                                    >  2:43706/1‑150 (MQ=255)
                                ggAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTAtt                                                                                                                      <  1:64891/148‑1 (MQ=255)
                                ggAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTg                                                                                                                   <  1:523051/151‑1 (MQ=255)
                                    aaaCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑a                                                                                                              <  2:646488/151‑1 (MQ=255)
                                     aaCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTATTTTGCg                                                                                                                 >  1:482270/1‑148 (MQ=255)
                                       cAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGTCGGCTGCGTGGCGGCCTGTTGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑ta                                                                                                          <  1:1418148/151‑2 (MQ=255)
                                        aaGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGt                                                                                                         <  1:546757/151‑1 (MQ=255)
                                        aaGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTTCTACTCCAAACACCGGCATTCCCTTTTTTTTGCGT‑AA‑t                                                                                                           >  1:136710/1‑149 (MQ=255)
                                        aaGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGt                                                                                                         >  2:9166/1‑151 (MQ=255)
                                          gTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTcc                                                                                                       <  2:76124/151‑1 (MQ=255)
                                            ctgggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTTCTCTGGCAACCACGGGCATGCCATTTATTTTGCGT‑AA‑TGTcc                                                                                                       >  2:134208/1‑149 (MQ=255)
                                            ctgggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAACCCCGGTCATGGCCTTTATTTTTCGT‑AA‑TGTTCGt                                                                                                     >  2:868159/1‑151 (MQ=255)
                                              gggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGttt                                                                                                   <  2:344077/151‑1 (MQ=255)
                                                 ctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGGCTGGC‑GGGGGGGTTTTCTCTGCCAAACACGGGCATGCCATTTATTCTGCGT‑AA‑TGTCCGttt                                                                                                   >  1:374821/1‑148 (MQ=255)
                                                   ggggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAg                                                                                              <  2:20650/151‑1 (MQ=255)
                                                       cAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGt                                                                                          <  2:574540/151‑1 (MQ=255)
                                                       cAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGGTTTTCTCTGCCCACCACCGTCCAGGCATTTTTTCTGCGG‑GA‑TGTCCGTTTTCCAggggg                                                                                          >  1:952393/1‑150 (MQ=255)
                                                        aaTTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTTTTTTTCCAAACCCGGGCATGGCATTTTTTTTGCGT‑AATTGTCCGTTTTCAGgggg                                                                                           >  1:584699/1‑150 (MQ=255)
                                                         aTTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTTCTCTTCCAACCCCGGGTATGGCCTTTTTTTTTCGTAAA‑TTTCCGTTT‑CAAgggg                                                                                           >  1:787412/1‑148 (MQ=255)
                                                            gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGTCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑GGTCCGTTTGCAAGGGGTGGAtt                                                                                     <  1:1082831/151‑1 (MQ=255)
                                                            gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTTCTTTGCCAAACACGGGTATGCCCATTATTTTGCGT‑AA‑TGTCCGGTTGCAAGGGGGGGAtt                                                                                     >  1:468184/1‑151 (MQ=255)
                                                               acCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAACCACGGGCATGGCATTTTTTTTGCGT‑AA‑TGTCCGTTTGCAAGgggggg                                                                                        >  1:259552/1‑145 (MQ=255)
                                                                 cGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCCGGC‑GGGGGGGTTTTCTCTGCCCACCACGGTCACGCCATTTTTTCTGCGT‑AA‑TGTCCGGTTGCCAGGGGTgggat                                                                                     >  1:308768/1‑143 (MQ=255)
                                                                       cGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAACCACGGGCATGCCATTTTTTCTGCGG‑AA‑TGTCCGGTTGCAAAGGGGGGATTCAGTAATg                                                                             >  2:1103686/1‑148 (MQ=255)
                                                                        gACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACg                                                                          >  2:38894/1‑150 (MQ=255)
                                                                         aCAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGcc                                                                        <  2:1377457/151‑1 (MQ=255)
                                                                           aaaGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTTCCTTTGCAAAGGGGGGATTTAGTAATGACGcc                                                                        >  2:1174697/1‑149 (MQ=255)
                                                                            aaGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGccacc                                                                     >  2:93874/1‑151 (MQ=255)
                                                                            aaGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGGCTGGC‑GGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTTTTCTGCGG‑AA‑TGTTCGTTTGGAAGGGGGGGGTTTAGTAATGAAGccacc                                                                     >  2:115132/1‑151 (MQ=255)
                                                                                gCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTTATCTTCCAAACACGGGCATGCCCTTTTTTTTGCGG‑AA‑TGTTCGGTTTCAAGGGGGGGGATCAGGAAATACGccacc                                                                     >  1:1027685/1‑147 (MQ=255)
                                                                                  tGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGTTTTACTCTGCCAACCACGGTCATGCCCTTTATTTTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGGAATGACCCCACCCGaa                                                                 >  2:1361766/1‑149 (MQ=255)
                                                                                   ggCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcgc                                                              <  2:95209/151‑1 (MQ=255)
                                                                                     cAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGGTTTACTCTGCCAACCCCGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGGGGGTTCAGTAATGACGCCACCAGAAcgcc                                                             >  2:248963/1‑150 (MQ=255)
                                                                                      aaaaGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcgccgc                                                           <  2:216202/151‑1 (MQ=255)
                                                                                      aaaaGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcgccgc                                                           <  2:1005957/151‑1 (MQ=255)
                                                                                       aaaGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCATGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCcgcg                                                          <  2:1301571/151‑1 (MQ=255)
                                                                                       aaaGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCcgcg                                                          <  2:51692/151‑1 (MQ=255)
                                                                                       aaaGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTTATTTTCCAACCACGGGCATGCCCTTTTTTCTTCGG‑AA‑TGTGCGGTTGCAAGGGGGGGGTTCAGAAAAGAAGCCACCAGAAAGCcgcg                                                          >  1:1212843/1‑151 (MQ=255)
                                                                                       aaaGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCCACCCCGGGCATGCCATTTTTTCTGCGT‑AA‑AGGGCGTTTGCAAGGGGGGGGTTTAGGAAATACGCCACCAGAAcgcc                                                             >  1:144857/1‑148 (MQ=255)
                                                                                        aaGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGG‑AA‑TGTCCGTTTTCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCcgcg                                                          >  2:1272496/1‑150 (MQ=255)
                                                                                         aGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCCACCACGGTCAATCCATTTTTTGTTGGT‑GA‑TGTTCGTTTGCACGGGGGGGGTTCAGTAATGACGCCCCCAGAAcgcc                                                             >  1:220156/1‑146 (MQ=255)
                                                                                             cTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGTTTTTCTCTGCCAAACACCGGCATGGCATTTATTTTGCGT‑AA‑AGTCCGTTTTCAAGGGGGGGGTTTAGGAATGAAGCCACCAGAACGCCGCGCACaa                                                     >  1:1269038/1‑150 (MQ=255)
                                                                                               ggCGCAAATGTATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAGCCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGcc                                                  <  1:102680/151‑1 (MQ=255)
                                                                                               ggCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGcc                                                  <  2:1398398/151‑1 (MQ=255)
                                                                                               ggCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTAATCTGCCCAACCCGGGCATGCCCTTTTTTCTTCGG‑AA‑AGTTCGGTTGCCAGGGGGGGGTTTAGTAATTAAGCCCCCCGAAAGCCccgccccaacc                                                  >  1:183549/1‑144 (MQ=255)
                                                                                                 cgcAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTg                                                <  2:1190125/151‑1 (MQ=255)
                                                                                                  gcAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCCACCACCGGCATGCCATTTATTCTGCGT‑AA‑TTTCCGTTTTCAAGGGGTGGATTCCGTAATGACGCCACCAGAACGCCGCGCACAAGCCCgg                                               >  2:830886/1‑151 (MQ=255)
                                                                                                    aaaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTAC‑CTGCCAACCAAGGGCATGCCCTTTTTTCTGCGT‑AA‑TGGCCGTTTTTCAGGGGTGGGTTTAGTAAAGTCGCCCCCCAGACGCCGCGCACCAGGCTTGcc                                             >  1:1298058/1‑150 (MQ=255)
                                                                                                    aaaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCa                                             <  1:1388967/151‑1 (MQ=255)
                                                                                                     aaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAg                                            <  2:391883/151‑1 (MQ=255)
                                                                                                       tGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAAACCCGGTCATGCCAATTTTTCTGCGT‑AA‑TGTCCCTTTGCAAGGGGGGGGTTCAGTAATGACGCCACCAGAACGCCGCGCACAAAACTGGCAGc                                           >  2:742112/1‑150 (MQ=255)
                                                                                                          aTTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACt                                       <  1:1413335/151‑1 (MQ=255)
                                                                                                          aTTACGGCGGCTGCGTGGCGGCCTGCGGTCTGGC‑GGGGGGGTTTTCTCTTTCCACCACGGTCATGCCCTTTTTTTTGGGG‑AA‑TAATCGTTTTCTAAGGGGGGGTTCCGGAATGAAAGCCCCTGAAAGCCGCGGACAAACCTGGCAGCGAc                                        >  1:592029/1‑150 (MQ=255)
                                                                                                            tACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTg                                      <  2:1245463/150‑1 (MQ=255)
                                                                                                            tACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGGGGGTTTACTCTGCCAACCACGGGCATGCCCTTTATTTTGCGT‑AA‑TGTCCGTTTGCAAGGGGGGGGTTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTgg                                     >  2:240096/1‑151 (MQ=255)
                                                                                                              cggcggCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTc                                   <  2:593270/151‑1 (MQ=255)
                                                                                                               ggcggcTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCg                                  <  2:1340689/151‑1 (MQ=255)
                                                                                                                 cggcTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGcc                                >  1:715214/1‑151 (MQ=255)
                                                                                                                 cggcTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGcc                                >  2:295025/1‑151 (MQ=255)
                                                                                                                     tGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGGTTTACTCTGCCAACCACGGTCATGCCCTTTATTCTGCGT‑AA‑TGGCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCCCCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGGCGCCGAtt                            >  1:1399671/1‑151 (MQ=255)
                                                                                                                       cGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTa                          <  1:1214346/151‑1 (MQ=255)
                                                                                                                              gcCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt                   <  2:505270/151‑1 (MQ=255)
                                                                                                                              gcCTGTGGTCTGGC‑GGGTGGTTTTACTATGGTAACTACGGTGATGCCATTTATTTTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGAGTCAGTAATGACGCAAACAGGAAGCCGCGGAAAAGCCTGGGAGCGACTGGTAGGCGATTTACCGGaa                    >  1:1119504/1‑150 (MQ=255)
                                                                                                                                  gtgGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAtt               <  2:1180618/151‑1 (MQ=255)
                                                                                                                                  gtgGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAt                <  1:178763/150‑1 (MQ=255)
                                                                                                                                   tgGTCTGGC‑GGGGGGTTTTACTCTGCCAACCACGGTCATGCGATTTATTCTGCGG‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGCAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGGTTTACCGGAATCa                 >  1:488385/1‑148 (MQ=255)
                                                                                                                                     gTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCta            <  2:31460/151‑1 (MQ=255)
                                                                                                                                     gTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCta            <  2:1236135/151‑1 (MQ=255)
                                                                                                                                     gTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCt             >  2:511372/1‑150 (MQ=255)
                                                                                                                                         ggC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATAcc        >  2:65605/1‑151 (MQ=255)
                                                                                                                                             gggTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATAccc       >  2:744702/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                        
CCTCGTGATGAGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGC‑GGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGT‑AA‑TGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATTCTATACCCAGGCG  >  NC_000913/3404072‑3404365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑