Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,508,6620TA37.0% 109.5 / 26.8 76E384D (GAA→GATyhfWphosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (10/36);  new base A (26/1);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.32e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGC  >  NC_000913/3508516‑3508805
                                                                                                                                                  |                                                                                                                                                    
gttgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAttta                                                                                                                                                   >  2:984930/1‑148 (MQ=255)
  tgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTcaca                                                                                                                                                 <  2:695365/150‑1 (MQ=255)
    tttttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTca                                                                                                                                                   >  2:1387661/1‑146 (MQ=255)
        tAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTACTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGCCGGGGGGAAAAAATCacacacag                                                                                                                                          >  2:401354/1‑147 (MQ=255)
         aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTTacacacag                                                                                                                                          >  2:795446/1‑146 (MQ=255)
         aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAg                                                                                                                                       <  1:428212/151‑1 (MQ=255)
             ccGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACGGGAGcccc                                                                                                                                  >  2:943343/1‑151 (MQ=255)
             ccGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGcccc                                                                                                                                  <  2:1305176/151‑1 (MQ=255)
              cGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGccccc                                                                                                                                 <  2:751629/151‑1 (MQ=255)
                gagagaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCcac                                                                                                                               <  2:927470/151‑1 (MQ=255)
                   agaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCAAAAACGGAAGcccc                                                                                                                                  >  1:1042511/1‑145 (MQ=255)
                    gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGGAAAAATTACACACGGGAGccccccc                                                                                                                               >  2:479439/1‑145 (MQ=255)
                    gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTCAAACACGGGAGccccc                                                                                                                                 >  1:672805/1‑145 (MQ=255)
                    gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATTAAACACCGGAGgcccccc                                                                                                                               >  2:1205601/1‑145 (MQ=255)
                       cAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGTGCGGCCGGGGAAATTCACACACGGGTACCCCCCCATc                                                                                                                            >  1:1000968/1‑147 (MQ=255)
                           atGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCg                                                                                                                    <  1:1154445/151‑1 (MQ=255)
                           atGCAGATACTCATGGGCGAGGGTGTAACCCGTCGTACCAAAACTCATTAGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAATAGCGACCATTATGTGGCGGTGGCGCGCGGAAAAATTGACACACGGTACCCCCCCCATCAGAAAGc                                                                                                                     <  1:651547/150‑1 (MQ=255)
                              cAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACGGGAACCCCCCCACCAGAAAGCgggg                                                                                                                 >  2:976031/1‑151 (MQ=255)
                               aGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGCGCGCGGGAAAAATCACACAAGGGAGCCCCCACATCAGAAAACgagg                                                                                                                 >  1:960423/1‑150 (MQ=255)
                                gATGCTCACGGGCGAGGGTGTAACCCGTCGGATGAAAACTCATAGGGTGTCTCCCGCAACCCGGAGGGAGGAAAGAAAAGATCGCCCATGTTGTGGCGGTGGCGCGCGGAAAAAGTCACACACCGTAGCCACCACATCAGAAAGCgtggtg                                                                                                               <  1:863923/151‑1 (MQ=255)
                                   gCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTgcg                                                                                                             <  2:383778/150‑1 (MQ=255)
                                   gCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTgcg                                                                                                             <  1:128152/150‑1 (MQ=255)
                                      cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGcaca                                                                                                         <  2:342696/151‑1 (MQ=255)
                                      cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATCACACACGGTAGCCCCCACATCAGAAAGCGTGGTgcg                                                                                                             <  1:1156695/147‑1 (MQ=255)
                                      cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGCGGGAAAAATCACACACGGTCGGCCCCCCCTCCGAAAGCGCGGGgcg                                                                                                             >  2:807942/1‑147 (MQ=255)
                                       aTGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGCGCGCGGGAAAAATCACACACGGGAGACCCCCCACCCGAAAAAGTGGTGGGGacac                                                                                                       >  1:1313209/1‑151 (MQ=255)
                                       aTGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGCCGCCGGAAAAATCATCAACAGTGGCCCCCCCACTAGAAAGcagggcgggggcac                                                                                                        >  2:1227994/1‑138 (MQ=255)
                                           gCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGGGCGCGGGAAAAATCACACACGGCAGCCCCCCCAACAGAAAAGGGGGTGCGGACAcc                                                                                                      >  2:1129894/1‑148 (MQ=255)
                                             gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGGGCGGGGGAAAAATTACACACGGGAGCCCCCCCCACCGAAAGCGTGGGGCGCACAcc                                                                                                      >  2:641379/1‑146 (MQ=255)
                                             gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCTCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGc                                                                                                  <  1:538872/150‑1 (MQ=255)
                                             gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACCGGAGCCCCCACAACAAAAAGCGTGGGGCGCACAcc                                                                                                      >  2:1108095/1‑146 (MQ=255)
                                                     aaCCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGc                                                                                          <  2:499868/150‑1 (MQ=255)
                                                          gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATAACACACGGGTGCCCCCCCATGCGAAAACGAGGGGCGGCCCCCGAGcccc                                                                                               >  1:35145/1‑137 (MQ=255)
                                                             ggATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATccc                                                                                 <  2:228893/151‑1 (MQ=255)
                                                               aTCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCtt                                                                               <  1:41077/151‑1 (MQ=255)
                                                                 cAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTg                                                                              <  2:1241206/150‑1 (MQ=255)
                                                                 cAAAACTCATAGGGTGTCGCCTACAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTGGTGGCGGTGGCGAGAGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTTCGTAGCGATCATCCCTTg                                                                              <  1:947913/150‑1 (MQ=255)
                                                                       tCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACCCACGGTAGCCCCCCCCTCAGAAAGCGTGGGGGGCACACCGAGCCGCGTAGCGGCCAACCCCTTCTGATa                                                                        >  1:1418722/1‑150 (MQ=255)
                                                                           aGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAcc                                                                    <  2:1328683/150‑1 (MQ=255)
                                                                            gggTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGTCCATTTTGTGGCGGTGGCGCTCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTTCGCACACCGAGCTGCTTAGCGATCATCCCTTACTGATAAACCa                                                                   <  1:1320691/150‑1 (MQ=255)
                                                                            gggTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAATTCACACACGGTAGCCCCCCCCTCAGAAAGGGTGGGGGGGACACCGCGCGGCGGAGCGATCATACCTTGCTGTTAAACCa                                                                   >  1:1044039/1‑150 (MQ=255)
                                                                              gtgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCa                                                               <  2:1374369/151‑1 (MQ=255)
                                                                                gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATTACACACGGTAGCCCCCCCCCCAAAAAACGTGGTGCGCACACCCAGCGGCGGAGCGATAATCCCTTGCTGTTAAAacaaca                                                               >  2:53768/1‑149 (MQ=255)
                                                                                 tctcCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGGGCGGGAAAAATCACACACGGTAGCCCCCACATCAGAAAGCGTGGGGCGCACACCCAGCTGCGGAGGGATCATCCCCTCCTGGTAAAcc                                                                    >  2:144651/1‑144 (MQ=255)
                                                                                    ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGca                                                         <  1:455213/151‑1 (MQ=255)
                                                                                    ccTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGca                                                         <  1:512781/151‑1 (MQ=255)
                                                                                       gCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTAGAGCGCGGGAAAACTCAAACCACGGAAACCCCACACCCGCAAGCCGGGGGCGCACACCCGGACTGGTAGATGTCATCCCCTGTTGATAAACCAGCACTGGcgcc                                                       >  2:675182/1‑150 (MQ=255)
                                                                                        cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCCGGGGAAAATTAACACACAGGAGCCCCCCCCACAGAAAAAGCGGGGGGCCCACCCAGCCGCGGAGCGGTCCTCCCCTCCTGATTAAACAGCCCCGGcgccac                                                     >  1:1000272/1‑151 (MQ=255)
                                                                                        cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACAAACAGGAGGCCCCACATCAGAAAACGGGGTGGCCACACCGAGCCGCGGATCGATCATCCCTTGTTTATAAAACAACACAGGcacca                                                      >  2:247018/1‑150 (MQ=255)
                                                                                        cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcaccac                                                     <  2:121986/151‑1 (MQ=255)
                                                                                         aaaCCGGAGGGAGGAAAGAAAAGAGCGCCCATTTTGTGGCGGTTGCGCGCGGAAAAATGCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCCCACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcaccac                                                     <  1:415446/150‑1 (MQ=255)
                                                                                             cGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGGGGGAAAAATCACACAAGGACGACCCCCAACCCCAAAAAGTGGTGAGCGCACCCAGCTGCGTAGCGAGAATCCCCTTCTGCTAAACAAGCACCGGcccccc                                                     >  1:1307374/1‑144 (MQ=255)
                                                                                               gagggaggTAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCTCACCCGGTAGCACCCGCATCAGAAAGCGGGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgcg                                               <  1:1187970/150‑1 (MQ=255)
                                                                                               gagggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGGGGGAAAAATTACAAACGGGAACCCCCACCTCAAAAAGCGGGGGGGGCACACCCAGCTTCCGAGCGATCATCCCTTGCTTTTAAAACAACACCTGGACCACCTCGCgg                                              >  2:1123416/1‑151 (MQ=255)
                                                                                                agggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTTGCGCCCGGAAAAAATCACACACCGTAACCCCCCCCTCCGAAAAAGTGGGGGGCCCACCCGACCGCGTCGCCCACATCCCCTGCTGATAAACCACCCACGGGCCCACCTCGCggg                                             >  2:1412252/1‑151 (MQ=255)
                                                                                                agggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATAACACACCGGAAGCCCCCCCTTAAAAAACGTGGGGCGGACACCCAAGAGCGGAGCGATTCTCCCCTTTTGTTAAAACAACACTGGCGCCACATCCCggg                                             >  1:287253/1‑151 (MQ=255)
                                                                                                 gggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGt                                            <  1:221843/151‑1 (MQ=255)
                                                                                                  ggaggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACCGGAGGCCCCCCATCCGAAAACGGGGGGGGGACACCGCGGCGCCGAGGGATCATCCCTTGCTGATAAAACAACACCGGGCCCACCTTGCgggg                                            >  2:245621/1‑149 (MQ=255)
                                                                                                  ggaggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTa                                           <  2:532652/151‑1 (MQ=255)
                                                                                                       aaagaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAATTTAAACACCGTAGCCCCCCCATCAAAAAGAGGGGGGCGCACACCCCGCTGCGGAGCGATCATCCCCTGCTGATAAAACAACACTGGCACCACTTCCCGGGGAttg                                        >  2:809944/1‑147 (MQ=255)
                                                                                                       aaagaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTAAACACAGGAGCCCCCACCACCGAAAGCGGGGGGGCCACACCCAGCTGCGTTGCGGTCATTCCCTTCTGGTAAACCAACACCGGGACCCCATCGCGGGGAtgg                                        >  1:1149192/1‑149 (MQ=255)
                                                                                                          gaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCt                                   <  2:1391757/151‑1 (MQ=255)
                                                                                                             aagaGCGACCATTTTGTGGCGGGGGGGCCCGGAGAAAATCACACACCGGGGACCCCCCCACAGAAAGCCTGGTGGGGGCCCCCAGCTGCGGAGGGATCCACCCCTGCTGCAAAAACAACACTGGCACCACCTAGCCGGGATGGTGGAtggg                                >  1:1300690/1‑149 (MQ=255)
                                                                                                              agagCGACCATTTTGTGGCGGGGTCGCCCGGAAAAAATCACACACCGGAGCCCCCCACACTGCAAACGTGGGGGGGACAACGAGACGCGGAGCGATGATCCCCCGCTGGTAAAACAGCACCGGCACCCCCTCCCgggggtggggggtg                                  >  1:417267/1‑137 (MQ=255)
                                                                                                                agCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGAcc                             <  2:222994/151‑1 (MQ=255)
                                                                                                                 gCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCa                            <  1:210384/151‑1 (MQ=255)
                                                                                                                     ccAGTTTGAGGCGGTGGCGCGCGGAAATACTCCCACACGGTAGCCCGCACATCAGAAAGAGGGGTGCGCACACCGCGCTGTGTAGCGTTCATTCCTCGCTGATAAACCAGCGCTGGCACCAGGTCGCGGGTATGGTGGCTGTGACCAATgg                        <  1:775435/151‑1 (MQ=255)
                                                                                                                              ggccgtggcgCGCGGAAAAATTCACGCACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACAACGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTTGCTGTGACCAATGGTCGGATCGt               <  1:1255091/151‑1 (MQ=255)
                                                                                                                               gcggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGtt              <  1:902166/151‑1 (MQ=255)
                                                                                                                               gcggtggcgCCCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCTTGGTGCGCACACCGAGCTGCGTAGCCCTCATCCCTTGCTGATAATCCAGCACTGTCACCTCTTCGCGGGTATGGTGGCTGTGCCCCATGTGCGGATCGtt              <  1:1203010/151‑1 (MQ=255)
                                                                                                                                cggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg             <  2:541604/151‑1 (MQ=255)
                                                                                                                                 ggtggcgCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg             <  1:1018014/150‑1 (MQ=255)
                                                                                                                                       gcgcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGAt      >  1:249509/1‑151 (MQ=255)
                                                                                                                                        cgcgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATc     <  1:859330/151‑1 (MQ=255)
                                                                                                                                          cgGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg   <  2:696686/151‑1 (MQ=255)
                                                                                                                                           ggAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGc  >  2:563581/1‑151 (MQ=255)
                                                                                                                                              aaaaTTCACTCACGGTAGGCCCCAAATCAGAGGGCGTGGTGCGAACACCGAGCTGCGTAGCGTTCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGGGGCTGGGACAAATGGTCGGATCGTGGCCGTGATCCGc  >  1:915655/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGTAGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGC  >  NC_000913/3508516‑3508805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: