Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,181,988	G→A	W298* (TGG→TAG)	yegV →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,181,988	0	G	A	94.6%	251.4 / ‑3.2	74	W298* (TGG→TAG)	yegV	putative kinase
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (24/46);  total (27/47)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAC  >  NC_000913/2181840‑2182133                                                                                                                                                     |                                                                                                                                                  gacgCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTagg                                                                                                                                                 <  2:841452/151‑1 (MQ=255)  acgCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTagg                                                                                                                                                 >  2:716240/1‑150 (MQ=255)       tCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTGAAACCCTTTGGGGCGGCCGACAGCAATGCCGGTGGCGTACTTGCCGGGCTGCCCTCTGGCCTGCCATTGGGGGATGCCGATTTTTTGGGCAATGAAGGGGCGCCGTAGGTTGTCg                                                                                                                                           >  2:939653/1‑151 (MQ=255)          ggCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGc                                                                                                                                        <  1:1307114/151‑1 (MQ=255)                aTTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGGTTTACTGGGCAATGCAGTGGCGTCGTGGGTGGGCggggctccgg                                                                                                                                  >  1:1149466/1‑144 (MQ=255)                    cGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCgg                                                                                                                              <  1:603473/151‑1 (MQ=255)                        aTTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTg                                                                                                                            >  2:1095201/1‑149 (MQ=255)                             cAACGCAAGTTTTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTgc                                                                                                                     <  1:1018812/151‑1 (MQ=255)                              aaCGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGGTGGCGGCTTACTCGGCTAGGCCGTGGCGTCGTGGGTGTTCGGGCGTCGGGGCGGGGAtt                                                                                                                         >  1:91364/1‑146 (MQ=255)                                  cAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCaa                                                                                                                <  2:833002/151‑1 (MQ=255)                                  cAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCaa                                                                                                                <  2:264170/151‑1 (MQ=255)                                       tGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAAcgcg                                                                                                            <  2:482639/150‑1 (MQ=255)                                          cGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAg                                                                                                        <  2:1042417/151‑1 (MQ=255)                                          cGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGGTGTCGGGGAACGGGGCGGGGATTGTGCGCCCAcccgcg                                                                                                          >  1:146271/1‑149 (MQ=255)                                           gACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCAACGCGCGAgg                                                                                                       >  2:1409496/1‑151 (MQ=255)                                              acCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAac                                                                                                    <  2:1407088/151‑1 (MQ=255)                                               ccATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAact                                                                                                   <  2:243063/151‑1 (MQ=255)                                               ccATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGGAATACTGGGGAATGCAGGGGCGTCGTAGGTTGTCGGGCCTCGGGGGGGGGGGTGTGGGCCCAcacccgggggaa                                                                                                    >  1:910489/1‑139 (MQ=255)                                                cATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAacta                                                                                                  <  2:997972/151‑1 (MQ=255)                                                 aTTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCCACGCGCGGGGGactac                                                                                                 >  2:1059454/1‑151 (MQ=255)                                                 aTTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCAACGCGCGAGGaa                                                                                                     >  2:172028/1‑147 (MQ=255)                                                      ggcgggcgACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTActcctc                                                                                            <  1:1167411/151‑1 (MQ=255)                                                            cgACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGcacac                                                                                      <  1:768815/151‑1 (MQ=255)                                                            cgACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAAAGCAGGGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGGTTGTGGGCCAACCCGCGAGGGACTACTCCCCGcac                                                                                        >  1:1189367/1‑149 (MQ=255)                                                              aCAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGAAGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACaa                                                                                    <  2:838544/151‑1 (MQ=255)                                                               cAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACaaa                                                                                   <  2:132635/151‑1 (MQ=255)                                                                agccATGCCGGTGGCGTTCTTGCCGGGCTGGCCTCTGGACTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGACGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCGACGCGCGAGGACCTTCTCCTCGCGCACaaa                                                                                   <  1:108082/147‑1 (MQ=255)                                                                aGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACaaaa                                                                                  <  1:732720/151‑1 (MQ=255)                                                                aGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACaaaa                                                                                  <  1:989456/151‑1 (MQ=255)                                                                 gTCATGCCTGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGGATTACTGGGCAATGCAGTGGCGTCGTAAGTTGTCGGGGATCGGGGCGGTGATTTTGCGCCAACCCGCGGGGAACCACTTCTCGCACACaaaa                                                                                  >  2:928044/1‑150 (MQ=255)                                                                   cATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAAc                                                                                <  2:665676/150‑1 (MQ=255)                                                                     tGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAAc                                                                                <  1:1167247/148‑1 (MQ=255)                                                                     tGCCGGTGGCGTACTTGCCGGGCGGGCGTCTGGTCTGGCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGt                                                                              <  1:532713/150‑1 (MQ=255)                                                                      gCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGtat                                                                            <  2:544336/151‑1 (MQ=255)                                                                         ggTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTTTTAATGGGCAATGAATGGGCGTCGGGGGTTGTCGGGCCTCCGGGCGGGGGTTGTGCGCCCACGCGCGGGGGACTACACCTCGCCCACaaaa                                                                                  >  1:1382045/1‑142 (MQ=255)                                                                          gTGGCGTACTTGCCGGGCTGGCCTCTGGTCGGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGAt                                                                        <  2:777788/151‑1 (MQ=255)                                                                            ggCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGCACCCGTAAACCTGGGTGACGCAGTTGCGCCGTAGGTTGTCGGGCATTGGGGCGGCGTGTGTGGGGCAACGCCTGAGGAACAAACCCCCCCCCCCAAAAACGta                                                                             >  1:221976/1‑144 (MQ=255)                                                                            ggCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATc                                                                       <  1:892565/150‑1 (MQ=255)                                                                            ggCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCg                                                                      <  2:564793/151‑1 (MQ=255)                                                                               gTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTg                                                                   <  1:1371419/151‑1 (MQ=255)                                                                               gTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTg                                                                   <  2:1173679/151‑1 (MQ=255)                                                                                 aCTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCg                                                                 <  1:1311071/151‑1 (MQ=255)                                                                                   ttGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCCACGCGCGAGGAAATACTCCTCGCACACAAAAAAAGATAGATCGCTGc                                                                  >  2:1160862/1‑148 (MQ=255)                                                                                     gCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                              <  2:187878/150‑1 (MQ=255)                                                                                     gCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGGATTACTGGGCAATGCAGTGGCGTCGTAGGGTGTCGGGCATCGGGGCGGGGGTTGTGCGCCCACCCGCGGGGGAATACTCCTCGCCCACAAAAAAGTATAGATCGCTGCg                                                                 >  1:1064636/1‑147 (MQ=255)                                                                                      ccGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGt                                                            <  2:645599/151‑1 (MQ=255)                                                                                      ccGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGt                                                            <  1:1404525/151‑1 (MQ=255)                                                                                        gggCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTgg                                                          <  2:589220/151‑1 (MQ=255)                                                                                           cTGGCCTCTGGTCTGCCACTGGCGGATGTCGTCTTTATCGGCAATGTAGTGGCGCCCGAGGTTGTCGGGCATCGGGGTGGTGAATGCTGGCCCACACGCGAGGGACTACTCCTCCCACCCAAAAAAGTATAAAGCCCCGCGACAGGGGCtc                                                       >  1:379586/1‑150 (MQ=255)                                                                                              gCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                    <  2:911801/151‑1 (MQ=255)                                                                                                  ctGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCt                                                  <  2:137088/149‑1 (MQ=255)                                                                                                   tGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGGGGCGTAGGCTGTCGGGCATCGGGGCGGGGGTTGTGCGCCAACCCGCGAGGAACTAATCCTCGCCCACAAAAACGGATAGATCGCTGCGGCAGTGGGTAAAGCtt                                                 >  1:180088/1‑148 (MQ=255)                                                                                                   tGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGGTTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGGTGCGGCAgggggcaaagct                                                  >  1:527367/1‑140 (MQ=255)                                                                                                         gctaCTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCa                                           <  2:623131/146‑1 (MQ=255)                                                                                                            aCTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAgg                                      <  2:1412136/151‑1 (MQ=255)                                                                                                            aCTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAgg                                      <  2:416184/151‑1 (MQ=255)                                                                                                               ggCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGc                                     <  1:538296/149‑1 (MQ=255)                                                                                                                gCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGGGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGt                                  <  1:100913/151‑1 (MQ=255)                                                                                                                 cGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGtt                                 <  1:1294750/151‑1 (MQ=255)                                                                                                                     tGCCGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTg                             >  2:463684/1‑151 (MQ=255)                                                                                                                       ccGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGtt                           <  2:117968/151‑1 (MQ=255)                                                                                                                        cGTATTACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGtt                           >  1:470948/1‑150 (MQ=255)                                                                                                                            ttACTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTACTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCCAATCCTGTACTCAATAGGCCGTTGCTGTTGGTc                       >  1:1027819/1‑150 (MQ=255)                                                                                                                             tACTGGGCAATGCTGTGGCGTCGTATGTTGGCGGGCATCGGGTCTGTGAGTGTGCGCCAACGCGCGATGAACTACTCCTCGCGCACCAAAACGTATAGATCGCGGCGACACTGGCTAATGCTGTACTCAATAGGGCGTTGCTGTTg                          <  1:1081999/146‑1 (MQ=255)                                                                                                                              aCTGGGCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCaa                     <  2:1282305/150‑1 (MQ=255)                                                                                                                                 gggCAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAgcg                  <  1:1296114/150‑1 (MQ=255)                                                                                                                                    cAATGCAGTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCAc              >  2:748243/1‑151 (MQ=255)                                                                                                                                           gTGGCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg       <  1:1148011/151‑1 (MQ=255)                                                                                                                                             ggCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGGCGTTGCTGTTGGTCAAGCGCCACTTGCTTGa      >  1:790418/1‑150 (MQ=255)                                                                                                                                              gCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGAt     >  2:1217476/1‑150 (MQ=255)                                                                                                                                              gCGTCGTAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATc    <  2:241294/151‑1 (MQ=255)                                                                                                                                               cgtcgtAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTTGTCAAGCGCCACTTGCTTGATc    >  1:1031037/1‑150 (MQ=255)                                                                                                                                               cgtcgtAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCa   <  1:323054/151‑1 (MQ=255)                                                                                                                                               cgtcgtAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACCCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCCGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg       >  2:82400/1‑147 (MQ=255)                                                                                                                                               cgtcgtAGGTTGTCGGGCACCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCa   <  1:1387019/151‑1 (MQ=255)                                                                                                                                                gtcgtAGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAc  >  2:504142/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GACGCTTCTGGCTGCATTCCGGCATTTCCAACGCAAGTTGTCGACACCATTGGGGCGGGCGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAC  >  NC_000913/2181840‑2182133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑