Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 82,372 T→C E386E (GAA→GAG leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091382,3720TC99.0% 360.1 / ‑4.4 97E386E (GAA→GAGleuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (39/56);  total (40/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01

Only 100 of 101 total aligned reads displayed.

TGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTT  >  NC_000913/82225‑82518
                                                                                                                                                   |                                                                                                                                                  
tgaatGATAGAGGACATCGACTGGACCGTTACCGTTGGCGGCTTCTGCTTTGACATCTTCGTCACAGGCCAGTTTGACGGCGGCGGAGGCGACATTGTACGAGCCATACTGCACGATGCAGACACCAAGACGGAAATGATCCGGCtcctct                                                                                                                                                 >  1:1258273/5‑151 (MQ=255)
tGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGctcctc                                                                                                                                                  <  1:1156854/150‑1 (MQ=255)
 gCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTcctctt                                                                                                                                                <  2:7303/151‑1 (MQ=255)
    tGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTtgct                                                                                                                                             >  1:1276945/1‑151 (MQ=255)
     gATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTtgct                                                                                                                                             <  1:501307/150‑1 (MQ=255)
        aGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCtt                                                                                                                                         >  2:704384/1‑151 (MQ=255)
         gACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCtt                                                                                                                                         >  2:151649/1‑150 (MQ=255)
         gACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTa                                                                                                                                        <  1:1244605/151‑1 (MQ=255)
          aCGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTAc                                                                                                                                       <  2:570052/151‑1 (MQ=255)
              cagcGACCGGACCGTTATCGTTGGCTGCTACTTCTTTGACTTCTTCGCCACAGGCCAGTTTGACTGCGGCAGTGGCGATATCTTTAGAGCCAGTCTGCGCGCTGAAGTAATGCAGACGCAAATGCTCCGGCTCCTCTTGCTGCTTACCGAt                                                                                                                                   <  1:1229968/148‑1 (MQ=255)
              cATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGAt                                                                                                                                   >  1:1080641/1‑151 (MQ=255)
              cATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGAt                                                                                                                                   <  2:145263/151‑1 (MQ=255)
              cATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGGTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGAt                                                                                                                                   >  2:614359/1‑151 (MQ=255)
                   accggaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAgg                                                                                                                              >  2:732636/1‑151 (MQ=255)
                     cggaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGcc                                                                                                                            <  2:944260/151‑1 (MQ=255)
                     cggaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGc                                                                                                                             >  2:1087661/1‑150 (MQ=255)
                               ccgttGGCGGCTTCTGCTTTGACTTTTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCa                                                                                                                  <  1:562085/151‑1 (MQ=255)
                                  ttGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTcc                                                                                                                   >  2:778002/1‑147 (MQ=255)
                                    ggcggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATc                                                                                                              <  2:1160982/150‑1 (MQ=255)
                                    ggcggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATc                                                                                                              <  2:1245550/150‑1 (MQ=255)
                                       ggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTa                                                                                                           >  1:27275/1‑150 (MQ=255)
                                        gcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGGCTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTa                                                                                                           <  1:587383/149‑1 (MQ=255)
                                           tCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATc                                                                                                        <  2:1076545/149‑1 (MQ=255)
                                           tCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCa                                                                                                       <  2:749753/150‑1 (MQ=255)
                                            cTGCTTTCACTTCTTCGCAACAGGCCAGTTTGACGGCGGAGGTGGCGATAACGTTAGAGCCAGACTGCACGCCGAAGTAATCCAGACGGAAATGCTCGGGCTCCTCCTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGGAAtta                                                                                                       >  1:848826/1‑147 (MQ=255)
                                              gCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAac                                                                                                    >  1:139999/1‑150 (MQ=255)
                                                   gACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACGCCTg                                                                                               <  1:349792/150‑1 (MQ=255)
                                                    aCTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGa                                                                                              <  2:562531/150‑1 (MQ=255)
                                                    aCTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGAc                                                                                             <  2:806661/151‑1 (MQ=255)
                                                    aCTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGAc                                                                                             >  2:734358/1‑151 (MQ=255)
                                                    aCTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGAc                                                                                             >  2:555568/1‑151 (MQ=255)
                                                      ttcttcGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCt                                                                                           >  1:622127/1‑151 (MQ=255)
                                                        cttcGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCt                                                                                           <  1:902870/149‑1 (MQ=255)
                                                           cGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCttttt                                                                                       <  2:355360/150‑1 (MQ=255)
                                                             ccACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGt                                                                                    <  2:1233281/151‑1 (MQ=255)
                                                              cacaGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTc                                                                                   >  2:1304498/1‑151 (MQ=255)
                                                                 aGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGc                                                                                >  2:1410726/1‑151 (MQ=255)
                                                                    ccAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAg                                                                             <  1:895779/151‑1 (MQ=255)
                                                                          tGACGTCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCa                                                                        <  1:308823/150‑1 (MQ=255)
                                                                            aCGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAgg                                                                      >  1:1067164/1‑150 (MQ=255)
                                                                            aCGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGa                                                                     <  2:325847/151‑1 (MQ=255)
                                                                             cggcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGa                                                                     >  1:645479/1‑150 (MQ=255)
                                                                             cggcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGa                                                                     >  1:409760/1‑150 (MQ=255)
                                                                              ggcggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGaaa                                                                   <  1:557849/151‑1 (MQ=255)
                                                                                 ggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCa                                                                <  2:1410795/151‑1 (MQ=255)
                                                                                  gcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCAt                                                               <  1:947123/151‑1 (MQ=255)
                                                                                     gTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGt                                                            <  2:1240631/151‑1 (MQ=255)
                                                                                         cGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACaa                                                        <  1:623527/151‑1 (MQ=255)
                                                                                          gATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACaaa                                                       <  1:457007/151‑1 (MQ=255)
                                                                                          gATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACaa                                                        >  2:1312535/1‑150 (MQ=255)
                                                                                           atatCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACaaa                                                       >  2:125413/1‑150 (MQ=255)
                                                                                           atatCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAAt                                                      <  2:1129517/151‑1 (MQ=255)
                                                                                            tatCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAAtt                                                     >  2:1328632/1‑151 (MQ=255)
                                                                                             atCGTTAGAGCCAGACTGCACGCTGAAGTACTCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTg                                                    <  2:785678/151‑1 (MQ=255)
                                                                                             atCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTg                                                    <  2:1265867/151‑1 (MQ=255)
                                                                                               cGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGt                                                   <  1:1295571/150‑1 (MQ=255)
                                                                                               cGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTc                                                  >  2:748457/1‑151 (MQ=255)
                                                                                                gTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCt                                                 <  1:690921/151‑1 (MQ=255)
                                                                                                  tAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTa                                                <  2:480070/150‑1 (MQ=255)
                                                                                                  tAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTa                                                <  2:27277/150‑1 (MQ=255)
                                                                                                  tAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTa                                                <  1:581476/150‑1 (MQ=255)
                                                                                                   agagCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTaa                                               <  1:975042/150‑1 (MQ=255)
                                                                                                   agagCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTaa                                               >  2:594921/1‑150 (MQ=255)
                                                                                                         aGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTACTCTTGATGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACACATTGTCTAAATTatat                                        >  1:80046/1‑151 (MQ=255)
                                                                                                         aGACTGCACGCTGAAGCAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTatat                                        >  1:653515/1‑151 (MQ=255)
                                                                                                          gACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATAtt                                       <  1:1079430/151‑1 (MQ=255)
                                                                                                                aCGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActtt                                 <  1:732602/151‑1 (MQ=255)
                                                                                                                aCGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActtt                                 <  1:539987/151‑1 (MQ=255)
                                                                                                                 cGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttc                                <  1:1346608/151‑1 (MQ=255)
                                                                                                                    tGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttcttt                             >  1:1201132/1‑151 (MQ=255)
                                                                                                                    tGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttc                                <  2:1399189/148‑1 (MQ=255)
                                                                                                                     gAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttcttt                             <  2:84598/150‑1 (MQ=255)
                                                                                                                     gAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttcttt                             <  2:439200/150‑1 (MQ=255)
                                                                                                                     gAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttctt                              <  2:120847/149‑1 (MQ=255)
                                                                                                                     gAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttctt                              <  2:551456/149‑1 (MQ=255)
                                                                                                                     gAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttctt                              <  1:727597/149‑1 (MQ=255)
                                                                                                                     gAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttctt                              <  2:1382090/149‑1 (MQ=255)
                                                                                                                     gAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCAccttctt                              <  2:1277029/149‑1 (MQ=255)
                                                                                                                         tAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGCCCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAcc                        >  1:1124199/1‑151 (MQ=255)
                                                                                                                         tAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAcc                        >  2:55224/1‑151 (MQ=255)
                                                                                                                          aaTCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCAAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAccc                       >  2:105871/1‑151 (MQ=255)
                                                                                                                          aaTACAGACGGAAATGCTCAGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAccc                       >  1:964649/1‑151 (MQ=255)
                                                                                                                             ccAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCAt                    <  2:611017/151‑1 (MQ=255)
                                                                                                                               tgACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGTCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTTAGGAAAGCATCGTACAAATTGACTAAATTATATTCACTTTCTTTATACCCCAtc                   >  1:883118/2‑150 (MQ=255)
                                                                                                                               aGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCAtct                  <  1:1273225/151‑1 (MQ=255)
                                                                                                                                gACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCAtctc                 >  1:775105/1‑151 (MQ=255)
                                                                                                                                 aCGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCAtctc                 >  2:747919/1‑150 (MQ=255)
                                                                                                                                 aCGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCa                <  2:587419/151‑1 (MQ=255)
                                                                                                                                   ggAAATGCTCCGGCTCGTCTTGCTGCTTACCGATGCAGGTCAGCGCCTACCGATCGTACTCAAACACCTGACCTGTTTTGTCCGTCAGTTTCGGGAAAGTATCGTACATATTGTCTATATTATATTCACTTTCTTTATACGCCATCTCAt               >  1:184403/1‑150 (MQ=255)
                                                                                                                                     aaaTGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATcc             >  2:890559/1‑150 (MQ=255)
                                                                                                                                     aaaTGCTCCGGCTCCTCTTGCTGCTTACCGACGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATc              >  1:34477/1‑149 (MQ=255)
                                                                                                                                       aTGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATg          <  2:140008/151‑1 (MQ=255)
                                                                                                                                       aTGCTCCGGCTCCTCATGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAATACCTGTTCTTTTTTAACCGGCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAAATATATGCACTTTCTTTATACCCCATCtcctcc             <  1:1371100/148‑1 (MQ=255)
                                                                                                                                          cTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCg        >  2:1374518/1‑150 (MQ=255)
                                                                                                                                          cTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCg        >  2:58917/1‑150 (MQ=255)
                                                                                                                                            ccGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGAt      <  1:537954/150‑1 (MQ=255)
                                                                                                                                            ccGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGAt      <  2:1043050/150‑1 (MQ=255)
                                                                                                                                            ccGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCAGCTCATCCATGCGAt      <  1:783769/150‑1 (MQ=255)
                                                                                                                                              ggCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGtt   >  2:344526/1‑151 (MQ=255)
                                                                                                                                                ctcctcTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTGTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAGATTATATTCACTTTCTTTATACCCCATCTCAGCCATGCAATGttt  <  1:651261/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATGTTT  >  NC_000913/82225‑82518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: