Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,524,685 T→A H65L (CAT→CTT)  xapA ← purine nucleoside phosphorylase 2; nicotinamide 1‑beta‑D‑riboside synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,524,6850TA99.1% 386.4 / NA 106H65L (CAT→CTT) xapApurine nucleoside phosphorylase 2; nicotinamide 1‑beta‑D‑riboside synthase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (57/47);  minor base G (1/0);  total (59/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 106 total aligned reads displayed.

CAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATA  >  NC_000913/2524538‑2524831
                                                                                                                                                   |                                                                                                                                                  
cAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGAc                                                                                                                                                 >  1:549464/1‑151 (MQ=255)
  gAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCa                                                                                                                                               <  2:213214/151‑1 (MQ=255)
    aCAGTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATg                                                                                                                                             <  2:1191960/151‑1 (MQ=255)
     cAGTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGt                                                                                                                                            <  2:364026/151‑1 (MQ=255)
       gTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTAc                                                                                                                                          <  1:1414181/151‑1 (MQ=255)
         aGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTAcc                                                                                                                                         <  2:25178/150‑1 (MQ=255)
         aGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCg                                                                                                                                        <  1:410889/151‑1 (MQ=255)
            tCGCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTAc                                                                                                                                     >  2:75969/1‑151 (MQ=255)
              gCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACtt                                                                                                                                   >  2:1191147/1‑151 (MQ=255)
               cAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACtt                                                                                                                                   <  2:1412990/150‑1 (MQ=255)
                  cccAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTAcc                                                                                                                                <  2:57868/150‑1 (MQ=255)
                   ccAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGATCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCgg                                                                                                                              >  2:1100869/1‑151 (MQ=255)
                    cagcagCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGTCCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTATCTCCCCCGTAAGACCATGTACCGTACTTACCg                                                                                                                               <  1:869617/149‑1 (MQ=255)
                     agcagcTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGCCCATGTACCGTACTTACCggg                                                                                                                             >  2:455519/1‑150 (MQ=255)
                       cagcTTAAAGGTCCGAATTGCGTCAGTCATAATGGTCATTACACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGa                                                                                                                            >  1:1401480/1‑149 (MQ=255)
                            tAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCgg                                                                                                                     >  1:219019/1‑151 (MQ=255)
                            tAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCg                                                                                                                      <  1:464057/150‑1 (MQ=255)
                              aaGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCa                                                                                                                   <  1:430913/151‑1 (MQ=255)
                               aGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGc                                                                                                                    >  1:698425/1‑149 (MQ=255)
                                  tACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCtt                                                                                                               >  2:1192524/1‑151 (MQ=255)
                                  tACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCt                                                                                                                <  2:926199/150‑1 (MQ=255)
                                   aCGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCttt                                                                                                              <  2:728099/151‑1 (MQ=255)
                                   aCGAATTGCGGCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTAAGCCCTGGAGATGGCCCAGCACTAACTACCCCGCAAGACCATGTACCGTACTTACCGCGAAGCCCGGCAGAttt                                                                                                              >  1:1289790/1‑151 (MQ=255)
                                       aTTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGt                                                                                                          <  1:732694/151‑1 (MQ=255)
                                        ttGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGt                                                                                                          >  1:1309442/1‑150 (MQ=255)
                                           cGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAgg                                                                                                       >  2:1339064/1‑150 (MQ=255)
                                              cagtcaTAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAAt                                                                                                   >  2:1150618/1‑151 (MQ=255)
                                               agtcaTAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAAtt                                                                                                  >  1:222634/1‑151 (MQ=255)
                                                gtcaTAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTg                                                                                                 <  2:615675/151‑1 (MQ=255)
                                                      aTGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGAc                                                                                             >  1:497533/1‑149 (MQ=255)
                                                        ggTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCg                                                                                         <  1:336183/151‑1 (MQ=255)
                                                         gTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGt                                                                                        <  1:176434/151‑1 (MQ=255)
                                                           cATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGtt                                                                                       <  2:692588/150‑1 (MQ=255)
                                                           cATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTtc                                                                                      >  1:1299516/1‑151 (MQ=255)
                                                            aTTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTtct                                                                                     >  2:235012/1‑151 (MQ=255)
                                                               ccACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCa                                                                                   >  1:936027/1‑150 (MQ=255)
                                                                cACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAATTGCGACAGCGTTCTCAATc                                                                                >  1:1423545/1‑151 (MQ=255)
                                                                   gACCTTCGTAGAAATGTCCGCGACTTTTTATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCTCAAGACCATGTACCGTACTTACCGGTAAGCCCGGCAGCTTTTCGTAGGAAATGGCGGCAGCGTTCTCAATcg                                                                               <  1:806453/150‑2 (MQ=255)
                                                                   gACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTg                                                                              <  1:711755/151‑1 (MQ=255)
                                                                   gACCTTCGCAGAAATGTCCGCGACCTTTCATCCATACCACCGGTACGCCCTGGAGATGGCCCAGCGCTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTg                                                                              >  1:380589/1‑151 (MQ=255)
                                                                     ccTTCGTAGAAATGTCCGCGACCTTGCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGAt                                                                            <  2:1331026/151‑1 (MQ=255)
                                                                      cTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATc                                                                           >  1:468065/1‑151 (MQ=255)
                                                                         cGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCg                                                                          <  1:1279772/149‑1 (MQ=255)
                                                                         cGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGc                                                                        >  2:8581/1‑151 (MQ=255)
                                                                          gTAGATATGTCCGCGACCGTTCATACAGACCTCCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTGCCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCAACAGCGTTCTCAATCTGATCGGcc                                                                       <  1:748118/151‑1 (MQ=255)
                                                                             gAAATGTCCGCGACCTTTCATACCTACCACCGGTACGCCCTGGAGAAGGCAGATTACTAACTCCCCCTCAGGACCATGTACAGTACTGACCGGGAACCCCGGCAGCATTTCGTAGGAAATTGCGACAGCATTCTCAATCTGATAGACCAgc                                                                    >  1:866742/1‑151 (MQ=255)
                                                                             gAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAgc                                                                    <  2:1069597/151‑1 (MQ=255)
                                                                              aaaTGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAgcg                                                                   <  1:306051/151‑1 (MQ=255)
                                                                              aaaTGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAgcg                                                                   >  2:949472/1‑151 (MQ=255)
                                                                              aaaTGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAgcg                                                                   <  1:859908/151‑1 (MQ=255)
                                                                                atgccggcgACCTTTCATGCATACCACCGGTTCGCTCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTGCTTACCGGGAAGCCCTGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGACAgcacg                                                                 <  1:681085/145‑1 (MQ=255)
                                                                                   tCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCg                                                              >  2:836264/1‑151 (MQ=255)
                                                                                    ccGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCTAGCGCGCCg                                                              >  1:1136967/1‑150 (MQ=255)
                                                                                    ccGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCg                                                              >  2:312407/1‑150 (MQ=255)
                                                                                    ccGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCg                                                              >  1:1403702/1‑150 (MQ=255)
                                                                                    ccGCGACCTTTAATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCg                                                              >  2:1202106/1‑150 (MQ=255)
                                                                                     cgcgACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAg                                                            <  2:1330585/151‑1 (MQ=255)
                                                                                      gcgACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGc                                                           >  1:1393157/1‑151 (MQ=255)
                                                                                        gACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGa                                                             >  2:7218/1‑147 (MQ=255)
                                                                                        gACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGccc                                                         >  1:131199/1‑151 (MQ=255)
                                                                                        gACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGccc                                                         >  2:174156/1‑151 (MQ=255)
                                                                                         aCCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCg                                                        >  2:667113/1‑151 (MQ=255)
                                                                                          ccTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCgg                                                       >  1:66865/1‑151 (MQ=255)
                                                                                             ttCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGa                                                      <  2:1414962/149‑1 (MQ=255)
                                                                                               catacataCCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCt                                                  >  1:304035/1‑151 (MQ=255)
                                                                                                       ccaccGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGTCCAGCGCGCCGAGCCCGGAACCTAAAATaaa                                          <  1:282629/151‑1 (MQ=255)
                                                                                                       ccaccGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATaaa                                          <  1:1340088/151‑1 (MQ=255)
                                                                                                       ccaccGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATaa                                           >  2:150217/1‑150 (MQ=255)
                                                                                                                   ccTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTTCCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTgg                              <  1:466138/151‑1 (MQ=255)
                                                                                                                   ccTGGAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTgg                              <  2:314121/151‑1 (MQ=255)
                                                                                                                      ggAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCg                            <  1:898203/150‑1 (MQ=255)
                                                                                                                      ggAGATGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCg                            <  2:779940/150‑1 (MQ=255)
                                                                                                                         gaTGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGa                         >  1:1374170/1‑150 (MQ=255)
                                                                                                                          aTGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGaaa                       <  2:1122660/151‑1 (MQ=255)
                                                                                                                           tGGCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAAt                      >  2:1176950/1‑151 (MQ=255)
                                                                                                                            ggCCCAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATc                     >  2:773956/1‑151 (MQ=255)
                                                                                                                              cccAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATc                     >  2:1323216/1‑149 (MQ=255)
                                                                                                                               ccAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAgg                  >  1:868668/1‑151 (MQ=255)
                                                                                                                                cAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGt                 <  1:993835/151‑1 (MQ=255)
                                                                                                                                cAGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGt                 >  1:86357/1‑151 (MQ=255)
                                                                                                                                cAGAACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTGCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGt                 >  1:454874/1‑151 (MQ=255)
                                                                                                                                 aGCACTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGtt                >  2:1009736/1‑151 (MQ=255)
                                                                                                                                    aCTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTtat             >  2:567373/1‑151 (MQ=255)
                                                                                                                                    aCTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTtat             <  2:920164/151‑1 (MQ=255)
                                                                                                                                     cTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTtat             >  1:998235/1‑150 (MQ=255)
                                                                                                                                     cTAACTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTtat             <  1:87239/150‑1 (MQ=255)
                                                                                                                                       aaCTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTtata            <  2:974717/149‑1 (MQ=255)
                                                                                                                                       aaCTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATaa           <  2:219029/150‑1 (MQ=255)
                                                                                                                                       aaCTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAg          <  1:347622/151‑1 (MQ=255)
                                                                                                                                       aaCTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAg          <  2:222036/151‑1 (MQ=255)
                                                                                                                                        aCTCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGt         <  1:846355/151‑1 (MQ=255)
                                                                                                                                          tCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCt       >  2:854818/1‑151 (MQ=255)
                                                                                                                                          tCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCt       >  2:867220/1‑151 (MQ=255)
                                                                                                                                          tCCCCCGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCt       >  2:522901/1‑151 (MQ=255)
                                                                                                                                           cccccGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCtt      <  1:377003/151‑1 (MQ=255)
                                                                                                                                            ccccGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTg     <  2:1054409/151‑1 (MQ=255)
                                                                                                                                            ccccGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTg     >  1:138163/1‑151 (MQ=255)
                                                                                                                                              ccGCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGat   >  2:718191/1‑151 (MQ=255)
                                                                                                                                                gCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGata  >  1:481744/1‑150 (MQ=255)
                                                                                                                                                gCAAGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGata  >  1:596626/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGAACAGTAGCTCGCAGCCCAGCAGCTTAAAGGTACGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATA  >  NC_000913/2524538‑2524831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: