Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,619,803	A→G	F120L (TTT→CTT)	uraA ←	uracil permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,619,803	0	A	G	97.6%	304.2 / ‑1.9	83	F120L (TTT→CTT)	uraA	uracil permease
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (44/37);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01

CAGGGTGGTAATAGAGATGATGATGGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAACAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATAAAGGCAAAG  >  NC_000913/2619663‑2619945                                                                                                                                             |                                                                                                                                               cAGGGTGGTAATAGAGATGATGATGGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTcc                                                                                                                                      >  2:909567/1‑151 (MQ=255)  aGGGTGGTAATAGAGATGATGATGGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTcc                                                                                                                                      >  1:1059521/1‑150 (MQ=255)  aGGGTGGTAATAGAGATGATGATGGTTTTGGAGTCCGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTcc                                                                                                                                      >  2:1169573/1‑150 (MQ=255)     gtggtAATAGAGATGATGATGGTTTTGGAGTCTGGCGTTTGCCCTTCAGCAGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTccagcc                                                                                                                                  >  1:509001/1‑151 (MQ=255)        gtAATAGAGATGATGATGGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCcagccagc                                                                                                                               >  2:786811/1‑151 (MQ=255)         tAATAGAGATGATGATGGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCagccagcc                                                                                                                              >  2:760024/1‑151 (MQ=255)         tAATAGAGATGATGATGGTTTTGGAGTCTGGCGATTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGCAGCAGCACGTccagccag                                                                                                                                >  1:280273/1‑149 (MQ=255)            tAGAGATGATGATGGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGt                                                                                                                           >  1:955597/1‑151 (MQ=255)                gatgatgatGGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAATCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTcccc                                                                                                                       >  1:420801/1‑151 (MQ=255)                gatgatgatGGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTcccc                                                                                                                       >  2:1239377/1‑151 (MQ=255)                  tgatgatgGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGc                                                                                                                     >  2:484596/1‑151 (MQ=255)                   gatgatgGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCt                                                                                                                    >  2:831669/1‑151 (MQ=255)                      gatgGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTttc                                                                                                                 >  2:1141109/1‑151 (MQ=255)                       atgGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTttct                                                                                                                >  1:1364049/1‑151 (MQ=255)                       atgGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTttct                                                                                                                >  1:819724/1‑151 (MQ=255)                        tgGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTttctt                                                                                                               >  2:682891/1‑151 (MQ=255)                            tttGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACg                                                                                                           >  1:19149/1‑151 (MQ=255)                                 aGTCTGGCGTTTGCCCTTCAACCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATa                                                                                                        >  1:385237/1‑149 (MQ=255)                                     tGGCGTTTGCCCTTCAGCCGGGAGTAAACACGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGa                                                                                                   >  1:318778/1‑150 (MQ=255)                                        cGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAAcc                                                                                               >  1:722632/1‑151 (MQ=255)                                              cccTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGc                                                                                           >  2:171558/1‑149 (MQ=255)                                               ccTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGc                                                                                           >  2:1076826/1‑148 (MQ=255)                                                 ttCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGGGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGaa                                                                                       >  1:1219841/1‑150 (MQ=255)                                                      ccGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCAc                                                                                 >  2:57415/1‑151 (MQ=255)                                                      ccGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCAc                                                                                 >  2:766201/1‑151 (MQ=255)                                                       ggggataacccccGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGGTTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAg                                                                                    <  2:884338/137‑1 (MQ=255)                                                         ggAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAACAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCAcgcc                                                                              >  1:329515/1‑151 (MQ=255)                                                            gTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCAcgccgc                                                                            >  2:1240735/1‑150 (MQ=255)                                                              aaaCCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGcac                                                                          >  2:442833/1‑150 (MQ=255)                                                                ccccGCCTTACCGGCAGTTTCTCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACata                                                                       <  2:1005127/150‑1 (MQ=255)                                                                ccccGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACat                                                                        <  2:821408/149‑1 (MQ=255)                                                                 cccGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACataa                                                                      <  2:1021864/151‑1 (MQ=255)                                                                 cccGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACata                                                                       >  2:981362/1‑150 (MQ=255)                                                                   cGCCATCCCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACataata                                                                    <  2:926124/151‑1 (MQ=255)                                                                    gCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACataata                                                                    >  1:252136/1‑150 (MQ=255)                                                                     ccATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACataata                                                                    <  1:562850/149‑1 (MQ=255)                                                                       aTACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATaaa                                                                  <  1:623159/149‑1 (MQ=255)                                                                         acgggCAGTGACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAgcc                                                               <  1:160503/147‑1 (MQ=255)                                                                         aCCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAgccg                                                              >  1:1358738/1‑151 (MQ=255)                                                                          ccGGCAGCTCCGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAgc                                                                <  1:661601/148‑1 (MQ=255)                                                                          ccGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAgccgc                                                             >  2:1224042/1‑151 (MQ=255)                                                                          ccGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAgccg                                                              <  2:634051/150‑1 (MQ=255)                                                                            ggCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGccc                                                           >  2:292132/1‑151 (MQ=255)                                                                              cAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCa                                                          <  2:1213861/150‑1 (MQ=255)                                                                              cAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCa                                                          <  2:331296/150‑1 (MQ=255)                                                                               aGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAgc                                                        >  1:211035/1‑151 (MQ=255)                                                                                gCTACGCCCGCCAGCACCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCa                                                          <  2:557082/148‑1 (MQ=255)                                                                                  tACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAgc                                                        <  2:512058/148‑1 (MQ=255)                                                                                       ccgccAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTc                                                >  1:504474/1‑151 (MQ=255)                                                                                       ccgccAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTc                                                <  2:544320/151‑1 (MQ=255)                                                                                          ccAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATa                                             <  2:1059589/151‑1 (MQ=255)                                                                                             gCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATAccc                                          >  2:745115/1‑151 (MQ=255)                                                                                                 cAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAAc                                      <  2:636472/151‑1 (MQ=255)                                                                                                  aGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAAc                                      >  1:614618/1‑150 (MQ=255)                                                                                                  aGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACg                                     <  2:490056/151‑1 (MQ=255)                                                                                                   gACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAAc                                      <  1:3850/149‑1 (MQ=255)                                                                                                   gACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACgg                                    >  2:849123/1‑151 (MQ=255)                                                                                                       gATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAAc                                <  1:374750/151‑1 (MQ=255)                                                                                                         tGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAGCCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACa                               <  1:1059971/150‑1 (MQ=255)                                                                                                         tGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAAc                                >  1:973171/1‑149 (MQ=255)                                                                                                          gACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGt                             <  2:1003327/151‑1 (MQ=255)                                                                                                            cGGCAACGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTaa                           <  2:1225443/151‑1 (MQ=255)                                                                                                                aaCGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAAt                       >  2:206634/1‑151 (MQ=255)                                                                                                                 aCGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCATCAGTAACAAt                       <  1:948725/150‑1 (MQ=255)                                                                                                                 aCGATTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATa                      <  1:1396032/151‑1 (MQ=255)                                                                                                                   gATTGCGCCCTTTGCCGCAGGGGGAAGCAGCACGTCCAGCCAGCCAGTCCCCTCTTTCTTCACGATACAAGAAACCAGGCAGAACAGCGCGGCGCACATAATAAAGCCGCCCAGCGCGGCTTCATACCCGAACGGCATCAGTAGCAAGAc                     <  1:229349/150‑1 (MQ=255)                                                                                                                    aTTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCg                   >  1:1291776/1‑151 (MQ=255)                                                                                                                    aTTGCGCCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCg                   >  1:911722/1‑151 (MQ=255)                                                                                                                       gcgcCCATTGCCGCAGGTGGAAACAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTg                <  2:178305/151‑1 (MQ=255)                                                                                                                         gcCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGa               >  2:294863/1‑150 (MQ=255)                                                                                                                         gcCCATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGa               >  2:1268336/1‑150 (MQ=255)                                                                                                                           ccATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGCTCCCCGCTTTCTTCACGATAAACGACACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGGCCAGCGCGACTTCATACCCTAACGCCGACAGTAACAATTCCGGTGa               <  1:115466/148‑1 (MQ=255)                                                                                                                            cATTGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATa           <  2:364138/151‑1 (MQ=255)                                                                                                                             aTTGCCGCAGGTGGAAGCAGCACGGCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATaa          <  1:1072419/151‑1 (MQ=255)                                                                                                                               tGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATaaa         <  1:715478/150‑1 (MQ=255)                                                                                                                               tGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATaaa         <  1:151536/150‑1 (MQ=255)                                                                                                                               tGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATaaa         <  1:551592/150‑1 (MQ=255)                                                                                                                               tGCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATaaa         <  2:955647/150‑1 (MQ=255)                                                                                                                                gCCGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATAAAgg       <  1:1240653/151‑1 (MQ=255)                                                                                                                                 ccGCAGGTGGCAGCCGCCCGTCCAGCCAGCCGGTCCCCGCTTTCCTCACGATAAAAGAAACCAGGCAGAACCGCACGCCGCACAGAATAAAGCCGCCCAGCGCGACTTCATTCCCTACCGGCAACAGTAACAATACCGGTGAAATAAAgg       <  1:32791/150‑1 (MQ=255)                                                                                                                                  cGCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATAAAGGCa     <  2:1181335/151‑1 (MQ=255)                                                                                                                                   gCAGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATAAAGGCa     >  1:713895/1‑150 (MQ=255)                                                                                                                                     aGGTGGAAGCAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATAAAGGCAAAg  <  1:937495/151‑1 (MQ=255)                                                                                                                                             |                                                                                                                                               CAGGGTGGTAATAGAGATGATGATGGTTTTGGAGTCTGGCGTTTGCCCTTCAGCCGGGAGTAAACCCGCCATACCGGCAGCTACGCCCGCCAGCTCCAGACCGATGACGGCAACGATTGCGCCCATTGCCGCAGGTGGAAACAGCACGTCCAGCCAGCCGGTCCCCGCTTTCTTCACGATAAAAGAAACCAGGCAGAACAGCACGCCGCACATAATAAAGCCGCCCAGCGCGACTTCATACCCTAACGGCAACAGTAACAATACCGGTGAAATAAAGGCAAAG  >  NC_000913/2619663‑2619945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑