Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,673,499 C→T P52S (CCA→TCA)  yphA → DoxX family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,673,4990CT98.5% 216.9 / ‑4.2 66P52S (CCA→TCA) yphADoxX family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (28/37);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

ACACTACGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGCCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACTGG  >  NC_000913/2673352‑2673645
                                                                                                                                                   |                                                                                                                                                   
acacTACGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCa                                                                                                                                                   >  1:801521/1‑150 (MQ=255)
acacTACGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCa                                                                                                                                                   >  1:381452/1‑150 (MQ=255)
  acTACGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGCCAATg                                                                                                                                                >  2:1077987/1‑151 (MQ=255)
  acTACGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCAGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATg                                                                                                                                                >  1:1149671/1‑151 (MQ=255)
   cTACGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGc                                                                                                                                               >  2:965573/1‑151 (MQ=255)
   cTACGCTATTTTGATTTTGTTGCTGCCCGTCCCGTTTTGTTATTAATTGCCCTTATCGCCGTGGTCTTAATTTTCTTTATTTTTGGTTTTCCCAAAATTATATGCTTTGATGGTACGGTCCAATTTATGGCCTCGTTGTGTGCGTCATTGc                                                                                                                                               >  1:133721/1‑151 (MQ=255)
    tACGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATTGGCTTTGACGGTACGGTCCAATATATCGCCTCGTTGGGCGCGTCAATGcc                                                                                                                                              >  1:215046/1‑151 (MQ=255)
     aCGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGcc                                                                                                                                              <  1:1315695/150‑1 (MQ=255)
     aCGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGc                                                                                                                                               <  2:1015217/149‑1 (MQ=255)
      cGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCg                                                                                                                                             >  2:1212121/1‑150 (MQ=255)
          attttgattttgGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCt                                                                                                                                        >  1:826020/1‑151 (MQ=255)
               gattttgGAGCCGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTGCCCAAAATGATGGGCTTTGACGGTGCGGTCTAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCa                                                                                                                                   <  1:429046/150‑1 (MQ=255)
                attttgGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAg                                                                                                                                  <  1:78142/150‑1 (MQ=255)
                attttgGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGc                                                                                                                                 >  1:150862/1‑151 (MQ=255)
                     ggAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGatta                                                                                                                            <  1:785251/151‑1 (MQ=255)
                           gcccgcccCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGc                                                                                                                        <  2:952794/149‑1 (MQ=255)
                           gcccgcccCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCgg                                                                                                                      <  2:713519/151‑1 (MQ=255)
                           gcccgcccCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCgg                                                                                                                      >  1:528465/1‑151 (MQ=255)
                             ccgcccCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGt                                                                                                                     >  2:847723/1‑150 (MQ=255)
                             ccgcacCGTTTGGTTATTAATTGCCCGTATCGCCGCGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGGCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTg                                                                                                                         >  1:666503/1‑146 (MQ=255)
                                ccccGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGtt                                                                                                                 >  2:1305416/1‑151 (MQ=255)
                                 cccGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTATGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTa                                                                                                                <  1:273334/151‑1 (MQ=255)
                                   cGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGCCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATg                                                                                                              <  1:992901/151‑1 (MQ=255)
                                    gTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATgg                                                                                                             <  2:638295/151‑1 (MQ=255)
                                     ttttGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATgg                                                                                                             >  1:564371/1‑150 (MQ=255)
                                      tttGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGAGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGa                                                                                                            <  2:92191/150‑1 (MQ=255)
                                       ttGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGaa                                                                                                           <  2:1423278/150‑1 (MQ=255)
                                          ttattaATTGCCCGTATCGCCGTGGGCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTg                                                                                                        >  2:1064381/1‑150 (MQ=255)
                                                     ccGTATCGCCTTGGTCTTATTTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGTTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGata                                                                                            <  1:893192/151‑1 (MQ=255)
                                                      cGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGatat                                                                                           >  2:434089/1‑151 (MQ=255)
                                                           cGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAAt                                                                                       <  1:599100/150‑1 (MQ=255)
                                                           cGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATc                                                                                      <  2:898087/151‑1 (MQ=255)
                                                                  gTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTGGACGGTACGGTCCAATATAGGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTg                                                                               <  2:966852/151‑1 (MQ=255)
                                                                      tAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTGCGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTgg                                                                              <  2:826056/148‑1 (MQ=255)
                                                                        aTTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCtttt                                                                         >  2:844354/1‑151 (MQ=255)
                                                                         ttttCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCttttt                                                                        >  2:13229/1‑151 (MQ=255)
                                                                          tttCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCTATGCTGGCAGCGATTATTGCGGTAGTTATGGAGGTGCCCGCCGCGATATTAATCGTGCTTGGCttttt                                                                        <  2:924282/150‑1 (MQ=255)
                                                                          tttCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTc                                                                       >  2:1029290/1‑151 (MQ=255)
                                                                            tCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCAc                                                                     >  1:1334625/1‑151 (MQ=255)
                                                                             cATTATTTTTGGTTTTCCCAAACTGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGGTTTTTCAcc                                                                    >  1:1364738/1‑151 (MQ=255)
                                                                                tattTTTGGTTTTCCCAAAATCATGGGCTTTGACGGTACGGTCCAATATTTCGCCTCGTTGGGCGCGTCAATGCCGAAGCTGGCAGCGACTAATTCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCAccc                                                                   >  1:1300900/1‑149 (MQ=255)
                                                                                   ttttGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGCCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGt                                                                 <  2:215056/148‑1 (MQ=255)
                                                                                    tttGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCg                                                              <  2:1313241/150‑1 (MQ=255)
                                                                                       ggTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTgg                                                          <  1:1380063/151‑1 (MQ=255)
                                                                                         ttttCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCg                                                        >  1:1416245/1‑151 (MQ=255)
                                                                                          tttCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCgg                                                       >  2:1410841/1‑151 (MQ=255)
                                                                                                aaaaTGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTg                                                  <  2:920138/150‑1 (MQ=255)
                                                                                                 aaaTGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTGATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGtt                                                <  1:757513/151‑1 (MQ=255)
                                                                                                        gggagttGACGGTGGGGTCTAATAGATGGCGGTGTTGGGGGGTTCAACGCCGATGTGGCAATTGATTGTTGCGGTAGTTTTGGAAGTGCGCGCCGCGATATTAAGCGTGCTTGGCTTTTTCCCCCGTCCGCTGGCGGTGCTGTTTATTTTc                                         <  1:33210/146‑1 (MQ=255)
                                                                                                          gCTTTGACGGAACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATAGCGGTAGTTATGGAGGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTa                                       <  2:772424/151‑1 (MQ=255)
                                                                                                           cttccacggaacgatCCAATATATGGCTTCTTTGGGCGCGTCAAGGCCAATGCTGGAGGCGATTATTGCGGTATTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCt                                        <  1:362176/144‑1 (MQ=255)
                                                                                                             ttGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTac                                      <  2:159173/149‑1 (MQ=255)
                                                                                                               gACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTTTTTTCTTCACGc                                  >  1:1081302/1‑151 (MQ=255)
                                                                                                                  ggtacggtCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTgg                               <  2:249993/151‑1 (MQ=255)
                                                                                                                     acggtCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTgggg                             >  1:347750/1‑149 (MQ=255)
                                                                                                                     acggtCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGt                             <  1:757706/150‑1 (MQ=255)
                                                                                                                     acggtCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGt                             <  2:494231/150‑1 (MQ=255)
                                                                                                                     acggtCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTa                            <  2:1149745/151‑1 (MQ=255)
                                                                                                                     acggtCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTa                            <  2:526251/151‑1 (MQ=255)
                                                                                                                        gtCCAATATATGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTTCACGcctggga                            >  1:454095/1‑142 (MQ=255)
                                                                                                                          caaaTATATGGCCTCGTTGGGCGCGTCAATGCGGATGCTGGCAGCGATTATGGCGGTAGTTATGGATGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTAcg                          <  1:387816/146‑1 (MQ=255)
                                                                                                                              tatatGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTg                    <  1:1285626/150‑1 (MQ=255)
                                                                                                                               atatGGCCTCGTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGa                   <  1:179165/150‑1 (MQ=255)
                                                                                                                                         gTTGGGCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGTCCGCAGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTCCACGCTGGGTAcggcggggcttt                >  1:406168/1‑137 (MQ=255)
                                                                                                                                           tGGGCGCGTCAATGCCGTTGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCAtt       <  2:1071056/150‑1 (MQ=255)
                                                                                                                                             ggCGCGTCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGGGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACt    <  1:925533/151‑1 (MQ=255)
                                                                                                                                                gcgtCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACTgg  <  1:603086/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACACTACGTTATTTTGATTTTGGAGCTGCCCGCCCCGTTTTGTTATTAATTGCCCGTATCGCCGTGGTCTTAATTTTCATTATTTTTGGTTTTCCCAAAATGATGGGCTTTGACGGTACGGTCCAATATATGGCCTCGTTGGGCGCGCCAATGCCGATGCTGGCAGCGATTATTGCGGTAGTTATGGAAGTGCCCGCCGCGATATTAATCGTGCTTGGCTTTTTCACCCGTCCGCTGGCGGTGCTGTTTATTTTCTACACGCTGGGTACGGCGGTGATTGGTCACCATTACTGG  >  NC_000913/2673352‑2673645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: