Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,850,439 C→T intergenic (‑4/‑208) hycA ← / → hypA regulator of the transcriptional regulator FhlA/protein involved in nickel insertion into hydrogenases 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,850,4390CT98.9% 338.9 / ‑3.7 90intergenic (‑4/‑208)hycA/hypAregulator of the transcriptional regulator FhlA/protein involved in nickel insertion into hydrogenases 3
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (41/48);  total (41/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01

AGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGC  >  NC_000913/2850294‑2850585
                                                                                                                                                 |                                                                                                                                                  
aGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGtt                                                                                                                                               >  1:883957/1‑151 (MQ=255)
aGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGt                                                                                                                                                <  2:825177/150‑1 (MQ=255)
 gCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGtt                                                                                                                                               >  1:970544/1‑150 (MQ=255)
    cATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTAcc                                                                                                                                            <  2:591880/150‑1 (MQ=255)
          gTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTa                                                                                                                                      >  1:782442/1‑150 (MQ=255)
             aTGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACag                                                                                                                                  <  1:838409/151‑1 (MQ=255)
                 aGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATGGTCATTGTTAGGTTACCCGTTTAACag                                                                                                                                  >  1:171964/1‑147 (MQ=255)
                 aGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGAt                                                                                                                              <  1:1133634/151‑1 (MQ=255)
                     gcgcTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCa                                                                                                                          <  2:125391/151‑1 (MQ=255)
                      cgcTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCa                                                                                                                          <  2:643866/150‑1 (MQ=255)
                       gcTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAg                                                                                                                         <  1:312461/150‑1 (MQ=255)
                         tttCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCtt                                                                                                                      >  1:633764/1‑151 (MQ=255)
                            cGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTa                                                                                                                    >  2:649098/1‑150 (MQ=255)
                            cGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTa                                                                                                                    >  2:388735/1‑150 (MQ=255)
                            cGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTa                                                                                                                    <  1:303513/150‑1 (MQ=255)
                            cGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTa                                                                                                                    <  1:781518/150‑1 (MQ=255)
                             gATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTa                                                                                                                    >  2:1145252/1‑149 (MQ=255)
                                aaCGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCAt                                                                                                               <  2:99997/151‑1 (MQ=255)
                                 aCGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCAtt                                                                                                              <  2:1093247/151‑1 (MQ=255)
                                  cGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCAttt                                                                                                             <  1:164000/151‑1 (MQ=255)
                                  cGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCAttt                                                                                                             >  2:697090/1‑151 (MQ=255)
                                    tGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCAttttt                                                                                                           >  1:1345142/1‑151 (MQ=255)
                                        ccccTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTtgt                                                                                                        <  2:785378/150‑1 (MQ=255)
                                        ccccTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTtgt                                                                                                        <  2:915479/150‑1 (MQ=255)
                                             gAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCaa                                                                                                   <  2:1149240/150‑1 (MQ=255)
                                             gAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCa                                                                                                    <  2:1359788/149‑1 (MQ=255)
                                             gAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAAc                                                                                                  <  1:947015/151‑1 (MQ=255)
                                             gAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAAc                                                                                                  >  2:522557/1‑151 (MQ=255)
                                               aCCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACtt                                                                                                >  2:1340282/1‑151 (MQ=255)
                                                ccAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACttt                                                                                               >  1:571117/1‑151 (MQ=255)
                                                  aGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACtttt                                                                                              >  1:1028531/1‑150 (MQ=255)
                                                    cGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAAt                                                                                           >  2:858116/1‑151 (MQ=255)
                                                      aaTTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAAttt                                                                                         <  1:1293759/151‑1 (MQ=255)
                                                           cAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGt                                                                                    <  2:1229564/151‑1 (MQ=255)
                                                              tAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGttat                                                                                 >  1:593305/1‑151 (MQ=255)
                                                               aGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGttat                                                                                 <  1:542197/150‑1 (MQ=255)
                                                               aGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGttat                                                                                 <  2:200027/150‑1 (MQ=255)
                                                               aGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGttat                                                                                 <  2:89602/150‑1 (MQ=255)
                                                                gATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGttatta                                                                               <  2:1253569/151‑1 (MQ=255)
                                                                gATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGttatt                                                                                >  2:899143/1‑150 (MQ=255)
                                                                 aTGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTaa                                                                              <  2:445342/151‑1 (MQ=255)
                                                                       cACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGAt                                                                        <  1:158284/151‑1 (MQ=255)
                                                                        aCTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGAtt                                                                       <  1:1113393/151‑1 (MQ=255)
                                                                           ggTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGAttttt                                                                    <  2:690137/151‑1 (MQ=255)
                                                                           gctCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAgagaatg                                                                      <  1:46792/147‑4 (MQ=255)
                                                                              ccTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTaa                                                                  <  1:1216296/150‑1 (MQ=255)
                                                                                tGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCt                                                               >  1:1117567/1‑151 (MQ=255)
                                                                                 gTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCt                                                               <  1:1294924/150‑1 (MQ=255)
                                                                                  tAGGAGACGCTGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAACTAGTCATTGTTAGGTTACCCGTTTCACAGAGCTGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTa                                                              >  1:789455/1‑150 (MQ=255)
                                                                                    ggCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAg                                                           <  1:1302713/151‑1 (MQ=255)
                                                                                         cgaTGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGa                                                       >  1:855060/1‑150 (MQ=255)
                                                                                          gaTGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAAt                                                     <  2:883999/151‑1 (MQ=255)
                                                                                             gCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTtc                                                  <  1:300867/151‑1 (MQ=255)
                                                                                              ccGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTtcg                                                 <  1:249541/151‑1 (MQ=255)
                                                                                              ccGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTtcg                                                 <  2:1262813/151‑1 (MQ=255)
                                                                                                   gtgCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTtcgtcg                                              <  1:541181/149‑1 (MQ=255)
                                                                                                         tGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCg                                      >  1:267760/1‑151 (MQ=255)
                                                                                                          gTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCg                                      <  1:713460/150‑1 (MQ=255)
                                                                                                           tAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGAc                                    >  2:495703/1‑151 (MQ=255)
                                                                                                            aaTCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACg                                   >  2:30772/1‑151 (MQ=255)
                                                                                                             aTCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACgt                                  <  2:257675/151‑1 (MQ=255)
                                                                                                                ggCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTc                               <  1:1170290/151‑1 (MQ=255)
                                                                                                                 gCTTTCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTc                               >  2:835999/1‑150 (MQ=255)
                                                                                                                   tttCTCGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCAtt                            >  2:51676/1‑151 (MQ=255)
                                                                                                                     tctcGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCAttt                           <  2:78486/150‑1 (MQ=255)
                                                                                                                     tctcGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTc                          >  2:512434/1‑151 (MQ=255)
                                                                                                                     tctcGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTc                          >  2:656733/1‑151 (MQ=255)
                                                                                                                        cGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTc                          <  2:941607/148‑1 (MQ=255)
                                                                                                                        cGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTc                          <  2:608837/148‑1 (MQ=255)
                                                                                                                        cGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGAc                       >  2:977957/1‑151 (MQ=255)
                                                                                                                        cGCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGAc                       >  1:145911/1‑151 (MQ=255)
                                                                                                                         gCTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGAca                      <  2:305776/151‑1 (MQ=255)
                                                                                                                          cTTATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGAcat                     >  1:75212/1‑151 (MQ=255)
                                                                                                                           ttATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGAcatc                    >  2:1213775/1‑151 (MQ=255)
                                                                                                                            tATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGAcatc                    >  1:196065/1‑150 (MQ=255)
                                                                                                                            tATTTCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGAcatc                    <  1:145651/150‑1 (MQ=255)
                                                                                                                               ttCCCAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCg                >  1:1392263/1‑151 (MQ=255)
                                                                                                                                 cccAAATAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGa               <  1:697058/150‑1 (MQ=255)
                                                                                                                                    aaaTAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACatt           >  1:1106979/1‑151 (MQ=255)
                                                                                                                                    aaaTAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACatt           <  1:521655/151‑1 (MQ=255)
                                                                                                                                     aaTAGTCATTGTTAAGTTACCCGCTTAACCGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGCTTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACa             >  1:319549/1‑148 (MQ=255)
                                                                                                                                      aTAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACattat         >  2:600441/1‑151 (MQ=255)
                                                                                                                                      aTAGTCATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACattat         >  2:1135709/1‑151 (MQ=255)
                                                                                                                                         gTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCa      <  2:1204878/151‑1 (MQ=255)
                                                                                                                                           cATTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCAcc    >  1:894214/1‑151 (MQ=255)
                                                                                                                                            aTTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCg   >  2:1119026/1‑151 (MQ=255)
                                                                                                                                            aTTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCg   <  2:103747/151‑1 (MQ=255)
                                                                                                                                            aTTGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCg   >  1:886339/1‑151 (MQ=255)
                                                                                                                                             ttGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGc  >  2:1382582/1‑151 (MQ=255)
                                                                                                                                              tGTTAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCCCCGc  <  1:501239/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AGCTCATGGCGTGATGCAGGCGCGCTTTCGATAACGTGATCCCCTGAACCAGCGAATTGCAGTAGATGTGCCACTGGTCCTGTAGGCGACGATGCCGCTGTGCGATGTAATCGGCTTTCTCGCTTATTTCCCAAATAGTCATTGTCAGGTTACCCGTTTAACAGAGATGCCAGCTTTAAGCATTTTTTGTGCCAACTTTTAATTTATTGTTATTAAAGAGATTTTTAAGCTAAAGATGAATTTCGTCGCCGTGTCGACGTGTCATTTCGACATCATCGACATTATTCACCGC  >  NC_000913/2850294‑2850585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: