Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,016,617 G→A L186L (CTG→CTA ygfK → putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,016,6170GA97.9% 353.0 / ‑1.8 95L186L (CTG→CTAygfKputative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (54/39);  total (56/39)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

GCCTTCTGATTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTGAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGA  >  NC_000913/3016470‑3016763
                                                                                                                                                    |                                                                                                                                                  
gCCTTCTGATTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAAt                                                                                                                                                 >  1:965993/1‑151 (MQ=255)
acctttctgttCCGGAAAACTCTTTCTTTTATAATAGGCGCGTCGTTAACAACCTCGAAGTTATAAAGCACCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTaaa                                                                                                                                                  <  2:803885/142‑1 (MQ=255)
       gATTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTAc                                                                                                                                          >  2:861805/1‑151 (MQ=255)
         ttCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTc                                                                                                                                        >  1:650670/1‑151 (MQ=255)
         ttCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTc                                                                                                                                        >  1:1285404/1‑151 (MQ=255)
          tCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACTGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTcc                                                                                                                                       >  1:1350766/1‑151 (MQ=255)
           cgaatAAATCTCTCTTTTTTAATATGGACCCCGTTAACAACCAGAAAGATATAAACCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACt                                                                                                                                         <  1:888083/144‑1 (MQ=255)
           ccGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTcc                                                                                                                                       >  1:243387/1‑150 (MQ=255)
               tAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGa                                                                                                                                   >  1:828501/1‑150 (MQ=255)
                aaaTCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATg                                                                                                                                 >  1:160731/1‑151 (MQ=255)
                       ttCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAt                                                                                                                           >  2:388770/1‑151 (MQ=255)
                       ttCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAt                                                                                                                           >  1:1018843/1‑151 (MQ=255)
                        tCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAtt                                                                                                                          >  2:102823/1‑151 (MQ=255)
                          aTCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAtttt                                                                                                                        >  2:1183200/1‑151 (MQ=255)
                           ttttaaaTATGAGCGTGGTTTCCACCCGGGAAGTTTTAAAGAAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAttt                                                                                                                         <  2:132071/144‑1 (MQ=255)
                           tCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAttt                                                                                                                         >  1:606919/1‑149 (MQ=255)
                             ttaaaTAGGGGCGTGGTTTACACCCTCGAAGTTATAAAGAAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAttt                                                                                                                         <  2:960816/144‑1 (MQ=255)
                                aaTATGGGCGTCGTTTACACCCTGAAAGGTATAAAACAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCt                                                                                                                   <  2:702859/150‑1 (MQ=255)
                                       gCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACgg                                                                                                           >  2:275206/1‑151 (MQ=255)
                                         gTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGAt                                                                                                         >  2:1191187/1‑151 (MQ=255)
                                          tcgttaacaccctgaaaGTTATAAAGCACCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGAt                                                                                                         <  2:624915/135‑1 (MQ=255)
                                            ggTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGc                                                                                                      >  1:930855/1‑151 (MQ=255)
                                            ggTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGc                                                                                                      >  2:1292617/1‑151 (MQ=255)
                                              ttaccgccTCGAGGGTACTAAGCAGCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCa                                                                                                     <  2:402535/144‑1 (MQ=255)
                                               tACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAgg                                                                                                   >  2:529784/1‑151 (MQ=255)
                                                acacCCTGGAGGTTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACg                                                                                                            <  2:875667/141‑1 (MQ=255)
                                                aCAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAgg                                                                                                   >  1:546867/1‑150 (MQ=255)
                                                  aaCCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGaa                                                                                                 >  2:318071/1‑150 (MQ=255)
                                                    ccgcGAAGTTATAAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACg                                                                                                            <  2:1082922/134‑1 (MQ=255)
                                                     cTCGAAGGTATTAAGCGACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAc                                                                                             >  1:452543/1‑151 (MQ=255)
                                                      tCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAcg                                                                                            >  2:1416198/1‑151 (MQ=255)
                                                       caaaGGTAAAAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAc                                                                                             <  2:807407/147‑1 (MQ=255)
                                                       cGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAcgc                                                                                           >  1:964140/1‑151 (MQ=255)
                                                           gttataaaaaaaCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGaaaaa                                                                                              <  1:303614/134‑1 (MQ=255)
                                                                 aaccacccgccgATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGc                                                                                 <  1:490711/145‑1 (MQ=255)
                                                                  aGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTGAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGc                                                                                 >  1:318339/1‑150 (MQ=255)
                                                                    cAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCaa                                                                               <  2:169724/150‑1 (MQ=255)
                                                                    cAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCa                                                                                >  1:322075/1‑149 (MQ=255)
                                                                      aCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGcc                                                                            >  2:288550/1‑151 (MQ=255)
                                                                      aCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGcc                                                                            >  1:680504/1‑151 (MQ=255)
                                                                      aCCGCCGACGGAACAGTTCATCGACAATATGATGGTCGCATCTTTCTATCCGAAATTCGCGCAATATCGCGATACGCTAAATAAATTACTCCAGTGTGACACATTTTTAGCTCGCCACGGATTGCATGAAAAACGCGAAAGCTTGCa                                                                                <  1:695350/147‑1 (MQ=255)
                                                                       ccgccgATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCt                                                                           >  1:466987/1‑151 (MQ=255)
                                                                        cgccgATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCtt                                                                          <  2:928405/151‑1 (MQ=255)
                                                                          ccgATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTAc                                                                        >  2:684326/1‑151 (MQ=255)
                                                                             aTGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCg                                                                     <  1:92204/151‑1 (MQ=255)
                                                                             aTGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCg                                                                     <  1:401638/151‑1 (MQ=255)
                                                                              tGCAACAGTTCATCGACAATATGATGGACGAATCTGACCATCGGAAATTCGCTCAATATCGTGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGCTTGCAGCAAAAACGCGAAAGCTTGCAAGCCTTAccc                                                                      >  2:109187/1‑149 (MQ=255)
                                                                               gCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGc                                                                    <  1:229032/150‑1 (MQ=255)
                                                                                ccaCAGTGCAGCGACAATATGCTCGCCGCATCTGACCGTCCGAAATGCGCTCAATATCGCGATACGCTAAACAAAATCCCCCAGGCTTCCGCACTTTTAGCTTGCCACGGATTGCAGGAAACACGCGCAAGCTTGCAAGCCTTCCCCGATc                                                                  <  1:1417176/149‑1 (MQ=255)
                                                                                cAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTCCTCCAGGATGACGCATTTTTAGCTCGCCACGGATGGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTc                                                                  <  2:490305/151‑1 (MQ=255)
                                                                                cAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTc                                                                  <  2:1134677/151‑1 (MQ=255)
                                                                                 aaCAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCg                                                                 >  2:1001319/1‑151 (MQ=255)
                                                                                   cAGTTCATCGAGAATATGATGTACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATTCGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCa                                                               <  1:407135/151‑1 (MQ=255)
                                                                                   cAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGc                                                                >  1:778034/1‑150 (MQ=255)
                                                                                   cAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGc                                                                >  2:308390/1‑150 (MQ=255)
                                                                                   cAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGc                                                                >  1:339189/1‑150 (MQ=255)
                                                                                    aGTTCGTCGAGAATATGATGGACGCATCTGATCATGCGAAATATGCTCAACATACCGATACGCTAATTAAATTACTTCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGACAAACGCGAACGCTTGCAACCCTTACCCGCTCGCa                                                               >  1:39215/1‑150 (MQ=255)
                                                                                    aGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCAt                                                              >  2:757715/1‑151 (MQ=255)
                                                                                     gTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTACAGGAAAAACGCGAAAGCTTGCACGCCTTACCCGCTCGCATc                                                             >  2:56766/1‑151 (MQ=255)
                                                                                      ttCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATcc                                                            >  1:959101/1‑151 (MQ=255)
                                                                                      ttCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATcc                                                            >  2:975537/1‑151 (MQ=255)
                                                                                        cATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCAGcc                                                            >  1:296963/1‑149 (MQ=255)
                                                                                           cGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCccacc                                                       >  2:267703/1‑151 (MQ=255)
                                                                                             aCAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCccacc                                                       >  2:718705/1‑149 (MQ=255)
                                                                                                atatGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTAt                                                  >  2:468291/1‑151 (MQ=255)
                                                                                                atatGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTAt                                                  <  2:174398/151‑1 (MQ=255)
                                                                                                atatGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTAt                                                  <  2:848249/151‑1 (MQ=255)
                                                                                                    gatgGACGCCTCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTg                                              <  1:497914/151‑1 (MQ=255)
                                                                                                      tgGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTGAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGc                                             >  2:944337/1‑150 (MQ=255)
                                                                                                       ggACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCa                                            <  1:967133/150‑1 (MQ=255)
                                                                                                       ggACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCAt                                           <  1:229447/151‑1 (MQ=255)
                                                                                                        gACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATg                                          >  2:341443/1‑151 (MQ=255)
                                                                                                           gCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCg                                       >  2:633934/1‑151 (MQ=255)
                                                                                                            cATCCGACCATCCGAAATTCCCTCAATATCGCGATACGCCAAATAAATTAATCCAGGATGACGCATTTTGAGCTCGCGACGGATTGCAGGAAAAACGCGCAAGCTTGCAAGCCTTACCCGCTGGCATCCCCACCAGTATGGTGCATGGCg                                       >  1:1318210/1‑150 (MQ=255)
                                                                                                              tCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTc                                     >  2:873438/1‑150 (MQ=255)
                                                                                                                 gACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCa                                    <  2:282823/148‑1 (MQ=255)
                                                                                                                 gACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCAccc                                 >  2:360316/1‑151 (MQ=255)
                                                                                                                 gACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCAccc                                 >  1:1318778/1‑151 (MQ=255)
                                                                                                                  aCCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCct                                >  1:492206/1‑151 (MQ=255)
                                                                                                                      tCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCtctc                             <  2:71449/150‑1 (MQ=255)
                                                                                                                           aaTTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCAt                       >  1:1369156/1‑151 (MQ=255)
                                                                                                                               cGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCAc                   <  1:308378/151‑1 (MQ=255)
                                                                                                                                  tCAATATCGCGATACGCCAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGc                >  1:924497/1‑151 (MQ=255)
                                                                                                                                   cAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCt               >  1:69617/1‑151 (MQ=255)
                                                                                                                                    aaTATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTg              <  2:867188/151‑1 (MQ=255)
                                                                                                                                    aaTATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTg              >  1:1336816/1‑151 (MQ=255)
                                                                                                                                     atatCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGt             <  2:1194584/151‑1 (MQ=255)
                                                                                                                                         cgcgATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGtcctc         <  1:1180091/151‑1 (MQ=255)
                                                                                                                                         cgcgATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGtcctc         <  1:670929/151‑1 (MQ=255)
                                                                                                                                         cgcgATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGtcct          <  1:1419903/150‑1 (MQ=255)
                                                                                                                                         cgcgATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGtcct          <  2:753776/150‑1 (MQ=255)
                                                                                                                                          gcgATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGtcctcc        <  1:1310168/151‑1 (MQ=255)
                                                                                                                                          gcgATACGCTAAATAAATTACTCCAGGAGGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGtcct          <  1:867470/149‑1 (MQ=255)
                                                                                                                                           cgATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCg       <  1:1199938/151‑1 (MQ=255)
                                                                                                                                               aCGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCGCCATGCACGGCTGTCCTCCGCATg   <  2:1269456/151‑1 (MQ=255)
                                                                                                                                                 gCTAAATAAATTACTCCAGGCAGACGCATTTCTAGCCCAAAACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCTTCCGCATGa  >  1:1196212/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCCTTCTGATTCCGGTAAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGCAACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTGAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGA  >  NC_000913/3016470‑3016763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: