Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,174,815 C→T L194L (TTG→TTA parE ← DNA topoisomerase IV, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,174,8150CT97.6% 281.0 / ‑3.1 82L194L (TTG→TTAparEDNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (41/39);  total (41/41)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

AGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTT  >  NC_000913/3174668‑3174961
                                                                                                                                                   |                                                                                                                                                  
aGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAAt                                                                                                                                                 <  1:621782/151‑1 (MQ=255)
   gAAATTACCGATAACCGGTTTTTCCGGCAGCTTCAGCAGACCATTTTCCGCTTCCGCCAGTTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAGCGCCAGGGCATAATAcc                                                                                                                                              <  1:381002/151‑1 (MQ=255)
   gAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATAcc                                                                                                                                              <  2:124675/151‑1 (MQ=255)
    aaaTTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATAcc                                                                                                                                              >  1:1163637/1‑150 (MQ=255)
    aaaTTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCg                                                                                                                                             >  1:778429/1‑151 (MQ=255)
     aaTTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGc                                                                                                                                            <  2:893172/151‑1 (MQ=255)
      aTTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACGGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAGAAGGGATCTCAACGCCAGGGCGTAATACCGc                                                                                                                                            >  1:281906/1‑150 (MQ=255)
      aTTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCg                                                                                                                                             <  1:300039/149‑1 (MQ=255)
      aTTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCt                                                                                                                                           >  1:1148523/1‑151 (MQ=255)
          ccGATAATCGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTgg                                                                                                                                       <  1:1349866/151‑1 (MQ=255)
          ccGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATCCCGCTTTgg                                                                                                                                       <  2:610374/151‑1 (MQ=255)
          ccGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTg                                                                                                                                        <  2:18161/150‑1 (MQ=255)
             aTAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCtt                                                                                                                                    >  1:381377/1‑151 (MQ=255)
             aTAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCtt                                                                                                                                    <  1:685911/151‑1 (MQ=255)
                 aCGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTc                                                                                                                                  >  2:145457/1‑149 (MQ=255)
                  cGGTTTTACCGACAGCGTCGGCAGACCATTTGCCGCTTCCGTCATGTGATCATTCAGAACGTCCTGATACCATCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAACTTCACTCAACGCCAGGGCATAATACCGGTTTGGCTTGCAg                                                                                                                                <  1:363140/150‑1 (MQ=255)
                     tttttCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGcac                                                                                                                             >  1:1327329/1‑150 (MQ=255)
                       tttCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACAt                                                                                                                           <  2:756944/150‑1 (MQ=255)
                         tCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGc                                                                                                                         <  1:168601/150‑1 (MQ=255)
                          ccGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCg                                                                                                                        <  2:26243/150‑1 (MQ=255)
                          ccGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCg                                                                                                                        >  2:1331691/1‑150 (MQ=255)
                              cAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAgg                                                                                                                   >  2:1355036/1‑151 (MQ=255)
                               aGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGc                                                                                                                  >  2:1263030/1‑151 (MQ=255)
                                  gTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTg                                                                                                               >  1:680500/1‑151 (MQ=255)
                                  gTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTg                                                                                                               >  2:381102/1‑151 (MQ=255)
                                  gTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTg                                                                                                               >  2:929599/1‑151 (MQ=255)
                                     ggCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGaaa                                                                                                            <  1:1244430/151‑1 (MQ=255)
                                         gACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAAc                                                                                                           >  1:1203424/1‑148 (MQ=255)
                                         gACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGa                                                                                                        <  2:427092/151‑1 (MQ=255)
                                           ccATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGaaa                                                                                                      <  1:1232188/151‑1 (MQ=255)
                                            cATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGaaa                                                                                                      >  1:1364753/1‑150 (MQ=255)
                                            cATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGaaa                                                                                                      >  1:47370/1‑150 (MQ=255)
                                                tACCGCTTCCGCTAGGTAATCATTCCGACCGTACTGATAGCACCAGCGTTGTTCGGTATTGTTGCTCTCATCTTTCAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGTCGTGAAACAGaaaa                                                                                                     >  1:720549/1‑147 (MQ=255)
                                                 aCCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATcgc                                                                                                 <  1:984826/150‑1 (MQ=255)
                                                  ccGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCgg                                                                                               <  2:1232350/151‑1 (MQ=255)
                                                  ccGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCgg                                                                                               <  2:1012203/151‑1 (MQ=255)
                                                  ccGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCgg                                                                                               <  1:260650/151‑1 (MQ=255)
                                                   cGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCggg                                                                                              >  2:1070214/1‑151 (MQ=255)
                                                    gCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGc                                                                                             >  1:505316/1‑151 (MQ=255)
                                                     cTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCt                                                                                            >  1:654220/1‑151 (MQ=255)
                                                      ttCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTg                                                                                           >  2:425629/1‑151 (MQ=255)
                                                        ccGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCt                                                                                            <  1:1398020/148‑1 (MQ=255)
                                                        ccGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTc                                                                                         >  2:173031/1‑151 (MQ=255)
                                                        ccGCCAGGTAATCATTCAGACCGTACTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTTGCTTTCAGCACATGCGTCAGGCGTCAAACAGAAAATCGCGGGCTGt                                                                                          >  1:1310169/1‑150 (MQ=255)
                                                             aGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCGTCATTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAaaga                                                                                    <  1:266373/151‑1 (MQ=255)
                                                             aGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAaaga                                                                                    <  2:813518/151‑1 (MQ=255)
                                                                 aaTCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGt                                                                                <  2:505340/151‑1 (MQ=255)
                                                                  ctCATTCAGACCGTACTGATAGCACCACCGTTGTTCGGTATAGTTGATCTCACCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGtt                                                                               >  1:327766/2‑151 (MQ=255)
                                                                              gTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAg                                                                   <  2:347669/151‑1 (MQ=255)
                                                                                  tGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGACACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGt                                                               >  2:474992/1‑151 (MQ=255)
                                                                                     aaGCATCATCGTTATTCGGTATTGTTGATCTGATCCTTAAAAGTGATCGCAACGCCAGGGCATAATACCACTTGGGCTTTCAGGACATGCCTCAGGCGTGAAGCAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGTCCAGTAGTGca                                                            <  1:1242051/150‑1 (MQ=255)
                                                                                       gCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGcaca                                                          <  2:891950/151‑1 (MQ=255)
                                                                                         aCCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACt                                                        >  2:758155/1‑151 (MQ=255)
                                                                                          ccAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTg                                                       >  2:1372786/1‑151 (MQ=255)
                                                                                             gCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTa                                                    <  1:36433/151‑1 (MQ=255)
                                                                                               gttgttCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTAcc                                                  >  2:612071/1‑151 (MQ=255)
                                                                                                  gttCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGt                                               >  2:82348/1‑151 (MQ=255)
                                                                                                     cGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTAtt                                            <  1:1237886/151‑1 (MQ=255)
                                                                                                     cGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTAtt                                            <  1:601852/151‑1 (MQ=255)
                                                                                                      ggTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTg                                           >  1:1122081/1‑151 (MQ=255)
                                                                                                      ggTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTg                                           <  2:544499/151‑1 (MQ=255)
                                                                                                               gATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTAcc                                   <  2:397168/150‑1 (MQ=255)
                                                                                                                aTCTCATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGc                                 >  2:1020741/1‑151 (MQ=255)
                                                                                                                   tcATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCa                                >  2:154015/1‑149 (MQ=255)
                                                                                                                    cATCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGt                             <  2:1163712/151‑1 (MQ=255)
                                                                                                                    cATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGc                                 <  2:527305/147‑1 (MQ=255)
                                                                                                                      tCTTTAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGt                             <  2:23383/149‑1 (MQ=255)
                                                                                                                         ttAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCg                         >  2:306482/1‑150 (MQ=255)
                                                                                                                         ttAAAAGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCg                         >  2:524931/1‑150 (MQ=255)
                                                                                                                          tAAAAGTGATCTCAACGCCAGGTCATAATACCGCTTTGGCTTTCAGCACATGCTTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGc                           <  1:1194613/147‑1 (MQ=255)
                                                                                                                           aaagGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTGTCAGCACATGCGTGAGACGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCTCACTGGTACCAGTATTGCGTTTACCGTAAGTGCGGAc                       <  1:997876/150‑1 (MQ=255)
                                                                                                                             aaGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAAc                    >  2:280369/1‑151 (MQ=255)
                                                                                                                             aaGTGATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAAc                    <  1:1340540/151‑1 (MQ=255)
                                                                                                                                 gATCTCAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTg                 <  1:665245/150‑1 (MQ=255)
                                                                                                                                   tctcAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGAGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTa               >  1:1353824/1‑150 (MQ=255)
                                                                                                                                    ctcAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTaa              >  1:146565/1‑150 (MQ=255)
                                                                                                                                    ctcAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTaa              >  2:736839/1‑150 (MQ=255)
                                                                                                                                    ctcAACGCCAGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTaa              >  2:1131308/1‑150 (MQ=255)
                                                                                                                                           ccAGGGCACAATACCGCGTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTGACCGCAAGTGCCGACAACCTGTAAATCCTGCa      <  1:38532/151‑1 (MQ=255)
                                                                                                                                            cAGGGCATAATACCGCTTTGGGTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCAc     >  1:1413547/1‑151 (MQ=255)
                                                                                                                                             aGGGCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCAcc    >  1:86387/1‑151 (MQ=255)
                                                                                                                                               ggCATAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCtt  <  2:1203500/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGTTTACCGCAAGTGCCGACAACCTGTAAATCCTGCACCTT  >  NC_000913/3174668‑3174961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: