Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,226,782 C→T D183D (GAC→GAT)  ygjI → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,226,7820CT98.7% 257.8 / ‑5.9 79D183D (GAC→GAT) ygjIputative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (39/38);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GACGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAG  >  NC_000913/3226638‑3226927
                                                                                                                                                 |                                                                                                                                                  
cGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCAt                                                                                                                                               <  1:440588/150‑1 (MQ=255)
 gCCTTCTCCACCTGGGTTTCCGCCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GACGCCAt                                                                                                                                               <  2:1319415/149‑1 (MQ=255)
    ttCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGGTGGCGGCGTACCGCCTGCT‑GATGCCATCAcc                                                                                                                                           >  2:304610/1‑150 (MQ=255)
        ccACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGGACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCCTCACCGTTg                                                                                                                                       >  2:1182191/1‑150 (MQ=255)
         cACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGAc                                                                                                                                     <  2:283279/151‑1 (MQ=255)
         cACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGAc                                                                                                                                     <  2:1208623/151‑1 (MQ=255)
                gTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCgatga                                                                                                                              <  2:852192/151‑1 (MQ=255)
                     caccaACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑Tccc                                                                                                                         >  2:208937/1‑150 (MQ=255)
                      accaACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGGTGA‑TCCCg                                                                                                                        >  1:411063/1‑150 (MQ=255)
                      accaACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCg                                                                                                                        >  2:685347/1‑150 (MQ=255)
                                cGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGGACGGGGCTGGTTGGCGGCGTACAGCCTGCT‑GGTGCCATCAACGTTGACGCGATGA‑TCCCGAACTTCAACt                                                                                                              >  1:968257/1‑150 (MQ=255)
                                 gAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGGTGGCGGCGTACAGCGTGCT‑GAAGCCATCACCGCTGACCCGATGA‑TCCCGAACTTCAACTgg                                                                                                            >  1:425413/1‑151 (MQ=255)
                                  aaaaTGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGTGACGGCGCTGGCTGGGGGCGGACAGCCCGCTGGATGCATTCACC‑TTGACGCGATGATTCCCCAAC‑TCCACTggg                                                                                                           >  1:273132/1‑151 (MQ=255)
                                  aaaaTGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGGAAGGCGCTGGTTGGCGGCGTAACGCCTGCT‑GATGTCATCACCGTTGAGGCCATGA‑TCCCGCACATCAACTggg                                                                                                           >  1:959319/1‑151 (MQ=255)
                                      ttttGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGtt                                                                                                       >  2:1398281/3‑151 (MQ=255)
                                      tGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGCTGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGATCGGCGCTGGTTTGCGGCGGATAGCCTGGT‑GATTCCCTCAGCGTTGACGCGATGC‑TCCCGCACTTCAACttg                                                                                                            >  1:1077150/1‑144 (MQ=255)
                                      tGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGGACGGCGCTGGTTGGCGGCGTACAACCTGCT‑GATGCCATCACCGTTGGCGCGGTGA‑TCCCGAACTTCCACTGGGCggt                                                                                                       >  2:1268494/1‑149 (MQ=255)
                                       gTTGGTGCCCTTTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGtt                                                                                                       <  1:310940/150‑1 (MQ=255)
                                          ggggCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTcc                                                                                                     >  1:537608/1‑149 (MQ=255)
                                          ggggCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTc                                                                                                   <  2:163412/151‑1 (MQ=255)
                                              ctaaTTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGc                                                                                                <  1:204167/148‑1 (MQ=255)
                                              ccAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCg                                                                                               >  1:889257/1‑151 (MQ=255)
                                                  ttACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTTGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGG‑TCCCGGACTTCAACTGGGCGGTCCTTGGCGTTac                                                                                           >  1:505583/1‑151 (MQ=255)
                                                            cACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGAt                                                                                  >  2:345918/1‑150 (MQ=255)
                                                                tCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GCTGCCATCACCGGTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCttt                                                                              >  1:416849/1‑150 (MQ=255)
                                                                 cAACGCTGATGCTCCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACCGCCTGCT‑GATGCCATCACCGTTGACCCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCttt                                                                              >  1:731443/1‑149 (MQ=255)
                                                                  aaCGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTAAGGCGCCGGTTGGCGGCGTACAGCCCGCT‑GATGCCATCACCGTTGACGCGAGGA‑TCCCGAAATTCAACTGGGGGTTCCTCGGCGTTACCCCCCGGGTCCTTa                                                                             >  1:19298/1‑149 (MQ=255)
                                                                   aCGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATgg                                                                          >  2:1078543/1‑151 (MQ=255)
                                                                     gCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGcc                                                                        <  2:618523/151‑1 (MQ=255)
                                                                      cTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGcc                                                                        >  2:811462/1‑150 (MQ=255)
                                                                           gctgctGTTAACGCTCTCCTACATTTTACTGGCAGGTAAGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGAAGCGATGA‑TCCCGAACTTCCACTGGGCGGTTCTCGGCGTTTCCACCTGGGTCTTTATGGCCGCa                                                                     >  1:125871/1‑148 (MQ=255)
                                                                            ctgctgTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTg                                                                 >  2:497444/1‑151 (MQ=255)
                                                                              gctgTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGgc                                                               <  1:806793/151‑1 (MQ=255)
                                                                               ctgTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAGCTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGgc                                                               <  1:683826/150‑1 (MQ=255)
                                                                                 gTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGgcg                                                              <  2:1294754/149‑1 (MQ=255)
                                                                                 gTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGGTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGcgcg                                                            >  2:209142/1‑151 (MQ=255)
                                                                                  ttAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGcgcg                                                            <  2:561785/150‑1 (MQ=255)
                                                                                     aCGCTCTCCTACATTTTACTGGCAGGGACGGCGCTGGGTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGGTCTTTATTGCCGCAGGGGGCGGGGAg                                                         >  1:1181877/1‑150 (MQ=255)
                                                                                       gCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTcc                                                      <  2:351288/151‑1 (MQ=255)
                                                                                          ctcCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCg                                                     <  1:1209544/149‑1 (MQ=255)
                                                                                            ccTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTc                                                   <  2:476590/149‑1 (MQ=255)
                                                                                               aCATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCt                                                <  2:900905/149‑1 (MQ=255)
                                                                                                 aTTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCtgtg                                             >  2:1183117/1‑150 (MQ=255)
                                                                                                 aTTTTACTGGCAGGGAAGGCGACGGTTGGCGGAGTACAGCCTGAT‑GATGTCATCACCGCTGACACGATGA‑TCCCGAACTTCAACTTGGCGTTCCTCGGCGCTACCACCTCGGTCTTTATGGCCCCCGGTGGCGCGGTGTCCGTCGCtgtg                                             >  1:1220585/1‑150 (MQ=255)
                                                                                                  ttttACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGGTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCtg                                               >  1:1251822/1‑147 (MQ=255)
                                                                                                  ttttACTGGCAGGTACGGCGCTGGTTGGCGGCGTAAAGGCTGCT‑GCTGCCATCACCGGTGACCCGGTGA‑TCCCCAACTTCAACTGGGCGGTCCTCCGCGGTACCACCTGGGTCTTTATGGCCGCAGGGGGCGGGGGGTGCGTCGCTgtgt                                            >  1:961435/1‑150 (MQ=255)
                                                                                                      aCTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGtt                                       >  2:523609/1‑151 (MQ=255)
                                                                                                      aCTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGt                                        <  1:847634/150‑1 (MQ=255)
                                                                                                      aCTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAAATGGGCGTTCCTCGGCGTTACCACCTGGATCTTTTTGGCCGCCGGTGGCGCGGAGGCCGTCGCTGTGTACGtt                                       >  1:638201/1‑151 (MQ=255)
                                                                                                       cTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGtt                                       <  2:224975/150‑1 (MQ=255)
                                                                                                          gCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAacg                                   <  2:430579/151‑1 (MQ=255)
                                                                                                          gCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAacg                                   <  2:97404/151‑1 (MQ=255)
                                                                                                           cAGGTACTGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTGCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAacga                                  <  1:1286515/151‑1 (MQ=255)
                                                                                                           cAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAacg                                   >  2:756514/1‑150 (MQ=255)
                                                                                                            aGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAacgac                                 >  1:699796/1‑151 (MQ=255)
                                                                                                            aGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAacgac                                 >  1:588142/1‑151 (MQ=255)
                                                                                                             ggTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAacgacg                                <  2:632193/151‑1 (MQ=255)
                                                                                                              gTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGt                               <  2:313469/151‑1 (MQ=255)
                                                                                                                 cGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCaa                            <  1:1199754/151‑1 (MQ=255)
                                                                                                                 cGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCaa                            <  2:547209/151‑1 (MQ=255)
                                                                                                                    cgcTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAgg                         >  1:1034227/1‑151 (MQ=255)
                                                                                                                    cgcTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAgg                         <  1:8452/151‑1 (MQ=255)
                                                                                                                      cTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGc                        <  1:593852/150‑1 (MQ=255)
                                                                                                                      cTGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCg                       >  2:495922/1‑151 (MQ=255)
                                                                                                                       tGGTTGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCgg                      >  1:1087070/1‑151 (MQ=255)
                                                                                                                           tGGCGGCGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTc                   <  2:273166/150‑1 (MQ=255)
                                                                                                                            ggcggcGTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGa                 <  2:1097984/151‑1 (MQ=255)
                                                                                                                              cggcgTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGaaa               <  2:404907/151‑1 (MQ=255)
                                                                                                                               ggcgTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAAt              <  1:345905/151‑1 (MQ=255)
                                                                                                                               ggcgTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGAGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAAt              >  2:453874/1‑151 (MQ=255)
                                                                                                                                gcgTACAGGATGCT‑GATGCCATCACCGTTGACGTGATGA‑TCCCGAACGTCAACTGGGGGGTCCTCGGCGTAACCACCTGAATCTTTATGGCCGCAGGTGGCGCGGAGTCTGACGCTGTGTACGTTGACTACGTCAAAGGCGGTTCGaaa               <  1:381036/149‑1 (MQ=255)
                                                                                                                                 cgTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAAtcg            >  2:669605/1‑151 (MQ=255)
                                                                                                                                  gTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAAtcgt           <  2:207301/151‑1 (MQ=255)
                                                                                                                                  gTACAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAAtcg            <  2:382822/150‑1 (MQ=255)
                                                                                                                                    aCAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAAtcgtt          <  2:1181951/150‑1 (MQ=255)
                                                                                                                                     cAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTCCCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGGTCGAAAtcgttcg        <  1:1370972/151‑1 (MQ=255)
                                                                                                                                     cAGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAAtcgttc         <  1:669573/150‑1 (MQ=255)
                                                                                                                                      aGCCTGCT‑GATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAAtcgttcgt       >  1:432956/1‑151 (MQ=255)
                                                                                                                                           gct‑gATGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAg  <  2:383871/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTGATGCTGCTGTTAACGCTCTCCTACATTTTACTGGCAGGTACGGCGCTGGTTGGCGGCGTACAGCCTGCT‑GACGCCATCACCGTTGACGCGATGA‑TCCCGAACTTCAACTGGGCGTTCCTCGGCGTTACCACCTGGATCTTTATGGCCGCAGGTGGCGCGGAGTCCGTCGCTGTGTACGTTAACGACGTCAAAGGCGGTTCGAAATCGTTCGTTAAAG  >  NC_000913/3226638‑3226927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑