Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,576,956 A→G S36P (TCC→CCC)  gntU ← gluconate transporter, low affinity GNT 1 system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,576,9560AG97.0% 352.3 / ‑1.9 101S36P (TCC→CCC) gntUgluconate transporter, low affinity GNT 1 system
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (49/49);  total (51/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

Only 100 of 101 total aligned reads displayed.

GCCGGTTTCATGTAAGATCTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCAC  >  NC_000913/3576813‑3577103
                                                                                                                                               |                                                                                                                                                   
gcCGGTTTCATGTAAGATCTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCaccacc                                                                                                                                              >  1:459582/1‑151 (MQ=255)
 ccGGTTTCATGTAAGATCTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCaccacc                                                                                                                                              >  1:890083/1‑150 (MQ=255)
     tttCATGTAAGATCTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAACAAAGGCCAGCCCCCATGGGCACCACCAtt                                                                                                                                           >  1:431094/1‑149 (MQ=255)
     tttCATGTAAGATCTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTa                                                                                                                                          >  2:920541/1‑150 (MQ=255)
        cATGTAAGATCTTGCCAAACATAGCTCCCAGGGCGCCACCCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTaaa                                                                                                                                        <  1:86312/149‑1 (MQ=255)
        cATGTAAGATCTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAg                                                                                                                                       <  1:449450/150‑1 (MQ=255)
         aTGTAAGATCTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGCGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGcc                                                                                                                                     >  2:733938/1‑151 (MQ=255)
           gTAAGATCTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCa                                                                                                                                    <  1:1025756/150‑1 (MQ=255)
           gTAAGATCTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCa                                                                                                                                    <  2:453735/150‑1 (MQ=255)
                  cTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCg                                                                                                                            >  2:717724/1‑151 (MQ=255)
                    tGCGAAGCATGGCTCCGAGGGCGGATGCCCGCGGCAGGAGGGCGAGGGTGCCTGCCAGCCATTTTTTCATCGTCGCTGCCGTTTTATCGAGCGGCTTTCGAAAAAAAAGGCGAGCCACCATGGACTCCACCATTAAAGCCAGGAAAg                                                                                                                              <  1:632641/147‑1 (MQ=255)
                    tGCCAAACATAGCTCCCAGGGCGACGACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGt                                                                                                                           <  1:843786/150‑1 (MQ=255)
                    tGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTg                                                                                                                          >  1:1138229/1‑151 (MQ=255)
                    tGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTg                                                                                                                          >  1:847037/1‑151 (MQ=255)
                     gCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGt                                                                                                                           <  2:1056130/149‑1 (MQ=255)
                      ccAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGc                                                                                                                         >  2:297182/1‑150 (MQ=255)
                      ccAAACATACCCCCCAGGGCGCCACCCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCCTCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCCCCATTAAAGCCAGGaaa                                                                                                                               <  1:1020294/144‑1 (MQ=255)
                        aaaCATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAACAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCgcg                                                                                                                          >  1:910237/1‑145 (MQ=255)
                        aaaCATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCa                                                                                                                        <  1:833177/149‑1 (MQ=255)
                             tAGCTCCCAGGGCGCCAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATAcgc                                                                                                                   <  1:1056024/149‑1 (MQ=255)
                               gCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCt                                                                                                               >  2:769107/1‑151 (MQ=255)
                                cTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCtt                                                                                                              >  1:1180366/1‑151 (MQ=255)
                                 tCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTc                                                                                                             >  2:1171493/1‑151 (MQ=255)
                                  cccAGGGCTACAACCCCCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCtt                                                                                                              <  1:180685/149‑1 (MQ=255)
                                  cccAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCtt                                                                                                              <  2:1101394/149‑1 (MQ=255)
                                    cAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATg                                                                                                          >  1:528866/1‑151 (MQ=255)
                                      gggCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGAc                                                                                                        >  1:1042604/1‑151 (MQ=255)
                                       ggCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACt                                                                                                       <  2:339181/151‑1 (MQ=255)
                                         cGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTa                                                                                                      >  2:1064079/1‑150 (MQ=255)
                                          gccAACCACCGCCAGGAAGCCGAAGGTGCCCCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACGTCCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTa                                                                                                      <  2:538399/147‑1 (MQ=255)
                                          gACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTaaa                                                                                                    <  1:1387376/151‑1 (MQ=255)
                                           aCAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTaaa                                                                                                    >  1:180510/1‑150 (MQ=255)
                                             aaCCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTaaa                                                                                                    <  1:151157/148‑1 (MQ=255)
                                                caccGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAAcagc                                                                                              <  2:1169118/151‑1 (MQ=255)
                                                caccGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAAcagc                                                                                              >  1:1204841/1‑151 (MQ=255)
                                                  ccGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAAcagca                                                                                             <  2:420877/150‑1 (MQ=255)
                                                   cGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAAcagcagc                                                                                           >  1:1183334/1‑151 (MQ=255)
                                                   cGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAAcagcagc                                                                                           <  1:1070595/151‑1 (MQ=255)
                                                     ccAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAAcagcagc                                                                                           >  1:803979/1‑149 (MQ=255)
                                                         gAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTaa                                                                                      >  2:155567/1‑150 (MQ=255)
                                                              cGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTaaa                                                                                     >  1:229358/1‑146 (MQ=255)
                                                               gAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGAc                                                                               <  2:1310184/151‑1 (MQ=255)
                                                               gAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGAc                                                                               >  1:1314590/1‑151 (MQ=255)
                                                               gAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGAc                                                                               >  2:395573/1‑151 (MQ=255)
                                                                aGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGAcc                                                                              >  2:319139/1‑151 (MQ=255)
                                                                         cccATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATCCCATAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAATAACAGCAGCAGTCAAACAGACCctgctgctg                                                                     <  1:484090/151‑1 (MQ=255)
                                                                         cccATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTActgctg                                                                     <  2:959504/151‑1 (MQ=255)
                                                                         cccATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTActgc                                                                       <  2:215005/149‑1 (MQ=255)
                                                                          ccATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGt                                                                    >  2:31929/1‑151 (MQ=255)
                                                                             tCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTa                                                                  >  1:2251/1‑150 (MQ=255)
                                                                             tCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTa                                                                  >  1:1342551/1‑150 (MQ=255)
                                                                              cccTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTaaa                                                                <  2:613852/151‑1 (MQ=255)
                                                                              cccTTTTTCCATAGTCGCTGCGATTTTATCGAGCGGCATACCCGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGGGCATACGCACCATCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTaa                                                                 >  1:877797/1‑150 (MQ=255)
                                                                               ccTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTaaa                                                                >  2:311896/1‑150 (MQ=255)
                                                                               ccTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTaaa                                                                >  1:322183/1‑150 (MQ=255)
                                                                                cTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATTCCAGAGAAAAGGCCATCCCCCCTGGGCACCGCCACTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGAACCTCCTGCTGTTaaa                                                                <  1:1218336/149‑1 (MQ=255)
                                                                                   tcgtcATCGTCTTTGCGATTTTATCGTGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAATCAGCAGCAGTAAAACAGTCCCTACTGCTGTTAAAAc                                                              <  1:1233498/144‑1 (MQ=255)
                                                                                    ttCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAg                                                           <  1:1074553/150‑1 (MQ=255)
                                                                                     tCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCg                                                         >  2:501400/1‑151 (MQ=255)
                                                                                      ccATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCg                                                         <  2:1099896/150‑1 (MQ=255)
                                                                                      ccATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGt                                                        <  2:18819/151‑1 (MQ=255)
                                                                                       cATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGtt                                                       >  2:501817/1‑151 (MQ=255)
                                                                                        aacggcgCTGCGATTTTATCGAGCGGCATAACAGAAAAAAGGCAAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTCCTGCTGTTAAAACAAGc                                                          <  1:938239/142‑1 (MQ=255)
                                                                                          cgtcgCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAAt                                                    >  1:1348303/1‑151 (MQ=255)
                                                                                             cgCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGt                                                  >  2:630766/1‑150 (MQ=255)
                                                                                               cTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGt                                               >  1:1104162/1‑151 (MQ=255)
                                                                                                     ttttATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCactac                                         >  1:584824/1‑151 (MQ=255)
                                                                                                         aTCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTa                                      <  2:1236295/150‑1 (MQ=255)
                                                                                                         aTCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTAt                                     >  2:1210740/1‑151 (MQ=255)
                                                                                                           cGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCactac                                         <  1:588490/145‑1 (MQ=255)
                                                                                                           cGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCactac                                         >  2:588521/1‑145 (MQ=255)
                                                                                                           cGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTAttt                                   <  1:1343208/151‑1 (MQ=255)
                                                                                                           cGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTAttt                                   >  1:813476/1‑151 (MQ=255)
                                                                                                           cGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTAtt                                    <  2:16959/150‑1 (MQ=255)
                                                                                                            gAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTg                                  <  1:825940/151‑1 (MQ=255)
                                                                                                               cGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCt                               >  1:952333/1‑151 (MQ=255)
                                                                                                               cGGCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCt                               >  2:937384/1‑151 (MQ=255)
                                                                                                                 gCATACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCttt                             >  1:210544/1‑151 (MQ=255)
                                                                                                                   aTACCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCttttt                           >  1:1386350/1‑151 (MQ=255)
                                                                                                                     aCCAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTta                         <  1:717690/151‑1 (MQ=255)
                                                                                                                      ccAGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTta                         >  1:937658/1‑150 (MQ=255)
                                                                                                                        aGAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTtaat                       >  2:1118026/1‑150 (MQ=255)
                                                                                                                         gAAAAAAGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACAACTTATTTGCCTTTTTtaataa                     <  2:151718/151‑1 (MQ=255)
                                                                                                                          aaaaaaGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAAc                    <  1:1064010/151‑1 (MQ=255)
                                                                                                                          aaaaaaGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAAc                    <  1:1393521/151‑1 (MQ=255)
                                                                                                                           aaaaaGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAAcc                   >  1:46221/1‑151 (MQ=255)
                                                                                                                            aaaaGGCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCt                  <  1:781977/151‑1 (MQ=255)
                                                                                                                                ggCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAAt              <  2:890126/151‑1 (MQ=255)
                                                                                                                                ggCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAAt              <  2:605488/151‑1 (MQ=255)
                                                                                                                                 gCCAGCCCCCATGGGCACCACCATTAAAGCCAGGACACCGTGCATACGCGCCTGCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGGTAAAACATGCGTAATTTTGGGCACTGCGTATTTGCATTTTTTAATAACCACAATg             <  1:744293/151‑1 (MQ=255)
                                                                                                                                 gCCAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATg             <  2:112328/151‑1 (MQ=255)
                                                                                                                                  ccAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATg             <  2:708951/150‑1 (MQ=255)
                                                                                                                                   cAGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGt           <  2:517918/151‑1 (MQ=255)
                                                                                                                                    aGCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTg          >  2:1192111/1‑151 (MQ=255)
                                                                                                                                     gCCCCCATGGGCACCACCATTAAAGCCAGGCAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGATAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGc         >  1:534779/1‑151 (MQ=255)
                                                                                                                                     gCCCCCATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGc         >  1:1051038/1‑151 (MQ=255)
                                                                                                                                       ccccATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCtt       <  1:503864/151‑1 (MQ=255)
                                                                                                                                        cccATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTg      <  2:1005063/151‑1 (MQ=255)
                                                                                                                                         ccATGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTCCCTTTTTTAATAACCTCAATGGTGCTTGc     <  2:350102/151‑1 (MQ=255)
                                                                                                                                            tGGGCACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCcac  >  2:1319079/1‑151 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GCCGGTTTCATGTAAGATCTTGCCAAACATAGCTCCCAGGGCGACAACCACCGCCAGGAAGCCGAGGGTGCCTCCCATCCCTTTTTCCATCGTCGCTGCGATTTTATCGAGCGGCATACCAGAAAAAAGGCCAGCCCCCATGGACACCACCATTAAAGCCAGGAAAGCGTGCATACGCGCCTTCATGACTAAAAACAGCAGCAGTAAAACAGACCCTACTGCTGTTAAAACAAGCGTTAATGTAGTCACTACTTATTTGCCTTTTTTAATAACCTCAATGGTGCTTGCCAC  >  NC_000913/3576813‑3577103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: