Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,923,204 G→A G159G (GGC→GGT rsmG ← 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,923,2040GA99.2% 428.3 / ‑4.2 117G159G (GGC→GGTrsmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (59/57);  total (59/58)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01

Only 100 of 117 total aligned reads displayed.

ATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTC  >  NC_000913/3923056‑3923350
                                                                                                                                                    |                                                                                                                                                  
attaaattTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGAcc                                                                                                                                                  >  2:1276719/1‑151 (MQ=255)
 ttaaattTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCt                                                                                                                                                 >  2:956435/1‑151 (MQ=255)
  taaattTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCt                                                                                                                                                 <  2:939536/150‑1 (MQ=255)
   aaattTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTg                                                                                                                                               <  1:348657/151‑1 (MQ=255)
    aattTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGc                                                                                                                                              >  1:1150750/1‑151 (MQ=255)
     attTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCt                                                                                                                                             <  1:458033/151‑1 (MQ=255)
      ttttATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTc                                                                                                                                            >  2:878735/1‑151 (MQ=255)
        ttATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCAc                                                                                                                                          >  1:75001/1‑151 (MQ=255)
         tATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGAGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCAcc                                                                                                                                         >  2:665529/1‑151 (MQ=255)
          aTTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCAcc                                                                                                                                         >  2:1394136/1‑150 (MQ=255)
           tttGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCAcc                                                                                                                                         >  1:2533/1‑149 (MQ=255)
           tttGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCAcc                                                                                                                                         >  1:686532/1‑149 (MQ=255)
           tttGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGAAGAAACGACCTTGATCAcc                                                                                                                                         >  1:712488/1‑149 (MQ=255)
            ttGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAg                                                                                                                                       <  1:1051502/150‑1 (MQ=255)
              gCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGaa                                                                                                                                    <  1:421069/151‑1 (MQ=255)
              gCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGaa                                                                                                                                    <  1:1204310/151‑1 (MQ=255)
                 ttAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCTCTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGa                                                                                                                                  <  1:369104/150‑1 (MQ=255)
                 ttAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGa                                                                                                                                     >  2:792797/1‑147 (MQ=255)
                  tAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGAtg                                                                                                                                >  1:898827/1‑151 (MQ=255)
                  tAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGAtg                                                                                                                                >  1:133549/1‑151 (MQ=255)
                   aaTCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGAtgg                                                                                                                               >  2:1210072/1‑151 (MQ=255)
                   aaTCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGAtg                                                                                                                                <  2:228990/150‑1 (MQ=255)
                    aTCACCACCAGGTGACGTTCGCCATCCAGGGCGGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGACCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGCtggt                                                                                                                              >  1:357291/1‑151 (MQ=255)
                      caccacCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGAtggtgg                                                                                                                            >  2:142998/1‑151 (MQ=255)
                      caccacCAGATGACGTTCGCCATCCAGGGCTGGAACATGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCTTTTGCCGTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGAtggtgg                                                                                                                            >  1:915051/1‑151 (MQ=255)
                       accaccAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGAtggtgg                                                                                                                            >  1:1047857/1‑150 (MQ=255)
                       accaccAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGc                                                                                                                           >  1:270768/1‑151 (MQ=255)
                             aGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCAcc                                                                                                                        >  1:1263939/1‑148 (MQ=255)
                             aGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGc                                                                                                                     >  1:668896/1‑151 (MQ=255)
                              gATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCt                                                                                                                    <  2:642488/151‑1 (MQ=255)
                               aTGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTc                                                                                                                   <  2:874970/151‑1 (MQ=255)
                                 gACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCa                                                                                                                  <  1:831538/150‑1 (MQ=255)
                                 gACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCAc                                                                                                                 >  2:516915/1‑151 (MQ=255)
                                  aCGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCAcc                                                                                                                >  2:308249/1‑151 (MQ=255)
                                    gTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCat                                                                                                              <  2:1303173/151‑1 (MQ=255)
                                     ttCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCat                                                                                                              >  1:728821/1‑150 (MQ=255)
                                         ccATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCg                                                                                                          <  1:350963/150‑1 (MQ=255)
                                           aTCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTc                                                                                                       <  2:1392204/151‑1 (MQ=255)
                                             ccAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCa                                                                                                      <  2:1057342/150‑1 (MQ=255)
                                              cAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGTGCAACAAAGCGATTTCATCTTCCGGCATCTGCCCTTTCAGCGCGTAGAAACGAACTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGtt                                                                                                        >  1:1417174/1‑147 (MQ=255)
                                              cAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTc                                                                                                       >  2:548296/1‑148 (MQ=255)
                                               aGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCag                                                                                                     >  1:737420/1‑149 (MQ=255)
                                               aGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCGCACCATATCGTTCag                                                                                                     >  2:1093502/1‑149 (MQ=255)
                                                 ggCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCagagag                                                                                                 <  2:1179728/151‑1 (MQ=255)
                                                  gCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAgg                                                                                                >  2:246727/1‑151 (MQ=255)
                                                  gCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAgg                                                                                                >  2:967697/1‑151 (MQ=255)
                                                   cTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGc                                                                                               >  2:1404887/1‑151 (MQ=255)
                                                    tGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGc                                                                                               >  1:93248/1‑150 (MQ=255)
                                                         ccTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAgc                                                                                         <  2:703656/151‑1 (MQ=255)
                                                         ccTGAAGTTTAACCACTGAGTCGACCTGATATTCTTCGGGCATCAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCAACAGCTCACCATATCGTTCAGAGAGGCaaaa                                                                                           <  2:267/149‑1 (MQ=255)
                                                             aaGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCgg                                                                                     <  1:143725/151‑1 (MQ=255)
                                                              aGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGc                                                                                    >  2:912219/1‑151 (MQ=255)
                                                               gTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCt                                                                                   <  2:270779/151‑1 (MQ=255)
                                                               gTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCt                                                                                   <  1:1112021/151‑1 (MQ=255)
                                                                tttAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCt                                                                                   >  1:799988/1‑150 (MQ=255)
                                                                 ttAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTa                                                                                  >  2:38718/1‑150 (MQ=255)
                                                                       aCTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGc                                                                           >  2:693249/1‑151 (MQ=255)
                                                                          gATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCa                                                                         <  1:1372988/150‑1 (MQ=255)
                                                                          gATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCAt                                                                        >  2:196269/1‑151 (MQ=255)
                                                                           actCGACCTGATATTCTTCGGGCAATAAAGCGATTTCATCTTCCGGCAGTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTCACCAGGAAGTTGGTGGCTCCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCAt                                                                        <  1:334434/148‑1 (MQ=255)
                                                                           aTTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCGAATTACGCCATc                                                                       >  2:1132026/1‑151 (MQ=255)
                                                                              cGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCaa                                                                     <  1:143352/150‑1 (MQ=255)
                                                                               gACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGGAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAAt                                                                   >  1:872939/1‑151 (MQ=255)
                                                                                aCCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATg                                                                  <  1:944422/151‑1 (MQ=255)
                                                                                     atatTCTTCGGGCAACAAAGCGATGTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATggcggc                                                             <  1:587609/151‑1 (MQ=255)
                                                                                         tcttcGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGctct                                                          <  1:264273/150‑1 (MQ=255)
                                                                                          cttcGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGctct                                                          >  2:143689/1‑149 (MQ=255)
                                                                                          cttcGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTg                                                         >  2:829949/1‑150 (MQ=255)
                                                                                             cGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAg                                                      <  2:651696/150‑1 (MQ=255)
                                                                                                gCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGaaa                                                  <  1:1021898/151‑1 (MQ=255)
                                                                                                 caacaaAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAAc                                                 <  1:368954/151‑1 (MQ=255)
                                                                                                  aacaaAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAAct                                                <  1:293003/151‑1 (MQ=255)
                                                                                                   acaaAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCGCCAGCTCACCATATGGTGCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGaaa                                                  <  2:520972/148‑1 (MQ=255)
                                                                                                   acaaAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAAct                                                <  1:1339112/150‑1 (MQ=255)
                                                                                                    caaAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAActct                                              >  2:339367/1‑151 (MQ=255)
                                                                                                             ttCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCt                                      <  2:1028861/150‑1 (MQ=255)
                                                                                                             ttCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCt                                      <  2:1169704/150‑1 (MQ=255)
                                                                                                                 tCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGctct                                 >  1:1303981/1‑151 (MQ=255)
                                                                                                                    tCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTg                                <  2:471414/149‑1 (MQ=255)
                                                                                                                    tCCGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTg                                <  1:141784/149‑1 (MQ=255)
                                                                                                                      cGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTTGCCCCAGCTCACTATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGt                               <  2:538568/148‑1 (MQ=255)
                                                                                                                      cGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACt                            >  1:627860/1‑151 (MQ=255)
                                                                                                                      cGGCATTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACt                            >  2:768645/1‑151 (MQ=255)
                                                                                                                          aTTTGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGtt                        >  1:635499/1‑151 (MQ=255)
                                                                                                                            ttGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTc                       <  2:243660/150‑1 (MQ=255)
                                                                                                                             tGCCCTTTCAGCGCGTAGACACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCa                      <  1:1219163/150‑1 (MQ=255)
                                                                                                                             tGCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCa                      <  1:985990/150‑1 (MQ=255)
                                                                                                                              gCCCTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCaa                     <  1:269076/150‑1 (MQ=255)
                                                                                                                                ccTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAatat                  <  2:1150824/151‑1 (MQ=255)
                                                                                                                                ccTTTCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAatat                  >  2:1142563/1‑151 (MQ=255)
                                                                                                                                   ttCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATAtt                 >  1:835270/1‑149 (MQ=255)
                                                                                                                                   ttCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATAtt                 <  2:1398844/149‑1 (MQ=255)
                                                                                                                                   ttCAGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATtc                <  2:221785/150‑1 (MQ=255)
                                                                                                                                      aGCGCGTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCa            <  1:914645/151‑1 (MQ=255)
                                                                                                                                         gcgTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGtt         >  2:617752/1‑151 (MQ=255)
                                                                                                                                          cgTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGttt        >  1:268588/1‑151 (MQ=255)
                                                                                                                                           gTAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGACATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTa       <  1:142989/151‑1 (MQ=255)
                                                                                                                                            tAGAAACGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTa       >  1:1148782/1‑150 (MQ=255)
                                                                                                                                               aaaCGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCt   <  1:693217/151‑1 (MQ=255)
                                                                                                                                                aaCGACCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTc  <  1:886423/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTCACCAGGAAGATGGTGGCACCAGCTCACCATATCGTTCAGAGAGGCAAAAGCGCGGCTAATTACGCCATCAAATGGCGGCTCTGAAGGAAACTCTTCTACCCTGCTCTGTACTGGTTCAATATTCTCCAGTTTAAGCTC  >  NC_000913/3923056‑3923350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: