Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,951,681 T→C pseudogene (140/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,6810TC98.8% 307.5 / ‑4.0 86pseudogene (140/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (50/35);  total (50/36)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

AAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGT  >  NC_000913/3951534‑3951825
                                                                                                                                                   |                                                                                                                                                
aaaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGACCATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACACCTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCggg                                                                                                                                               <  2:910699/151‑1 (MQ=255)
  aTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGgc                                                                                                                                             <  2:1368355/151‑1 (MQ=255)
  aTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGgc                                                                                                                                             <  1:939692/151‑1 (MQ=255)
  aTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGgc                                                                                                                                             >  1:130522/1‑151 (MQ=255)
      aTGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGgcagc                                                                                                                                          >  1:1297266/1‑150 (MQ=255)
       tGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCAc                                                                                                                                        >  2:644935/1‑151 (MQ=255)
        gACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCAcc                                                                                                                                       >  2:1277889/1‑151 (MQ=255)
          cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCAcc                                                                                                                                       >  2:1072114/1‑149 (MQ=255)
          cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCAcc                                                                                                                                       >  1:576643/1‑149 (MQ=255)
          cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCAcc                                                                                                                                       >  1:135083/1‑149 (MQ=255)
              cagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGAtg                                                                                                                                  >  1:459480/1‑150 (MQ=255)
               agcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGAtgtg                                                                                                                                >  2:122772/1‑151 (MQ=255)
                  aaCACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGc                                                                                                                             >  1:891834/1‑151 (MQ=255)
                    cacTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTg                                                                                                                           <  1:862120/151‑1 (MQ=255)
                        gcgcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTgcgc                                                                                                                        <  1:476097/150‑1 (MQ=255)
                        gcgcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTgcg                                                                                                                         <  2:815150/149‑1 (MQ=255)
                          gcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCgcag                                                                                                                      >  2:897083/1‑150 (MQ=255)
                            gcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGcac                                                                                                                   >  1:1072855/1‑151 (MQ=255)
                             cAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGcaca                                                                                                                  <  1:67193/151‑1 (MQ=255)
                              aGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACAt                                                                                                                 >  2:382607/1‑151 (MQ=255)
                              aGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACAt                                                                                                                 <  2:1229538/151‑1 (MQ=255)
                                 tccgcgatcaACATTCCTGGCGTTACGACCATCCCGGCGACGCTATCTACGCGCCGCTGTTGTTAAGACAACTGCCGGATCGCAAACCTGCGGATCGCGTCGTGACCAAAGATGCGAGGGAGCACCAGTTGTGGGCTTCgcagc                                                                                                                     >  1:155426/6‑144 (MQ=255)
                                 tGCGTGATGAACATTACTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGCTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGc                                                                                                              >  1:281547/1‑151 (MQ=255)
                                           aCATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGc                                                                                                    <  2:331345/151‑1 (MQ=255)
                                             aTTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGccc                                                                                                  <  1:211546/151‑1 (MQ=255)
                                             aTTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGccc                                                                                                  >  1:511638/1‑151 (MQ=255)
                                              ttCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGCTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCg                                                                                                 >  2:422324/1‑151 (MQ=255)
                                                ccTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCgg                                                                                                >  1:1253387/1‑150 (MQ=255)
                                                  tGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGaa                                                                                              >  2:1170839/1‑150 (MQ=255)
                                                   ggCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGaaaa                                                                                            <  2:842230/151‑1 (MQ=255)
                                                   ggCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGaa                                                                                              <  2:1113032/149‑1 (MQ=255)
                                                        tACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTtc                                                                                        >  2:678853/1‑150 (MQ=255)
                                                          cGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTtcatc                                                                                     <  2:283882/151‑1 (MQ=255)
                                                                 cccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTcc                                                                               >  2:447613/1‑150 (MQ=255)
                                                                 cccGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTcc                                                                               <  1:432644/150‑1 (MQ=255)
                                                                         cGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGttt                                                                       >  2:1156973/1‑150 (MQ=255)
                                                                            tATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAg                                                                     <  2:728940/149‑1 (MQ=255)
                                                                            tATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGt                                                                   >  1:398402/1‑151 (MQ=255)
                                                                              tCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTAc                                                                 >  1:1170498/1‑151 (MQ=255)
                                                                               cTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTAcc                                                                >  2:765161/1‑151 (MQ=255)
                                                                                 acgcatcggtgttgttAAAACGACTGTCGGATCGGAAACCTGCGGATTGGTTCGTGACCACAGATGCGGGGCAGAACCAGATGTGGGCTGCGCAGCACATCGCCCACTCTCGCCCGGAGAATTTCATCACCTCCAGCGGTTTAGGTACCa                                                               <  1:1238570/141‑1 (MQ=255)
                                                                                 aCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTAcc                                                                <  1:497977/149‑1 (MQ=255)
                                                                                  cgcgCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATg                                                             >  1:674664/1‑151 (MQ=255)
                                                                                  cgcgCCGTTGTTGTTAAAACAACTGTCGGAGCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATg                                                             <  2:796120/151‑1 (MQ=255)
                                                                                    cgcCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATggg                                                           <  2:45044/151‑1 (MQ=255)
                                                                                     gcCGTTGTTGTTAAAACAACAGTCGGATCGTAAACCTGCGGATTGCGGCGTGAAAACAGATACGGGGCAGCACCAGATGGCGTCGGCGGAGCACATCGCACACACTCGCCCGGAAAATTTCATCACATCCAGCGGTTTAGGTACCATgg                                                            >  1:864670/1‑149 (MQ=255)
                                                                                      ccGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTTGGCTGCACAGCACATCGCCCACACTCGACCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGt                                                          >  1:457929/1‑150 (MQ=255)
                                                                                         ttgttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTg                                                      >  2:204575/1‑151 (MQ=255)
                                                                                          tgttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTg                                                      <  2:1118391/150‑1 (MQ=255)
                                                                                           gttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGt                                                    >  1:962337/1‑151 (MQ=255)
                                                                                           gttgttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACGCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTgg                                                     <  1:265661/150‑1 (MQ=255)
                                                                                               ttAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCCCACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTa                                                 <  2:686273/150‑1 (MQ=255)
                                                                                                tAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTAcc                                               >  2:1076981/1‑151 (MQ=255)
                                                                                                tAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTAc                                                <  2:830473/150‑1 (MQ=255)
                                                                                                 aaaaCAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcg                                              >  1:122583/1‑151 (MQ=255)
                                                                                                 aaaaCAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcg                                              >  2:828936/1‑151 (MQ=255)
                                                                                                  aaaCAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcgg                                             >  1:434/1‑151 (MQ=255)
                                                                                                   aacaacTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcggc                                            >  2:1077669/1‑151 (MQ=255)
                                                                                                    acaacTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcggcg                                           >  2:435094/1‑151 (MQ=255)
                                                                                                      aacTGTCGTATCGTAAACCTGCGGACTGCGTCGTGACCACAGATGCGGCGCAGCACCAGATGTGGGCTGCGCAGCACATCGCGCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGGTTTGGTTTACccgcggc                                         >  1:782590/1‑151 (MQ=255)
                                                                                                      aacTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCGCCAGATGTGGGCTGCGCAGCACAGCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcggcggc                                         <  1:1177087/151‑1 (MQ=255)
                                                                                                      aacTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcggcggc                                         >  1:130757/1‑151 (MQ=255)
                                                                                                      aacTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcggcggc                                         <  2:169335/151‑1 (MQ=255)
                                                                                                        cTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACcggcggcgg                                       >  2:170677/1‑151 (MQ=255)
                                                                                                          gTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGtt                                     <  1:1061162/151‑1 (MQ=255)
                                                                                                          gTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGtt                                     <  2:1260041/151‑1 (MQ=255)
                                                                                                            cGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTg                                    <  2:795516/150‑1 (MQ=255)
                                                                                                                   tAAACCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTTGCGcaca                             >  2:1005798/1‑150 (MQ=255)
                                                                                                                    aaaCCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTTGGGCACaa                            >  1:1370384/1‑150 (MQ=255)
                                                                                                                    aaaCCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACaa                            <  1:50751/150‑1 (MQ=255)
                                                                                                                    aaaCCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGGTTTGGTTTACCGGCGGCGGGGGGCGTACaa                            >  1:98405/1‑150 (MQ=255)
                                                                                                                     aaCCTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGt                          <  2:46697/151‑1 (MQ=255)
                                                                                                                       ccTGCTGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCAGATCGCCCACCCTCGCCCGGAAAATTTCATCACTTCCAGCGGTTTAGGTCCTATGGGTTTTGGTGTACCGGCGGCGGTTGGCGCACAAGTc                         <  1:1101099/150‑1 (MQ=255)
                                                                                                                       ccTGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTcg                        >  2:1075669/1‑151 (MQ=255)
                                                                                                                         tGCGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTcgcg                      >  2:468809/1‑151 (MQ=255)
                                                                                                                           cGGATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTcgcgc                     >  1:663241/1‑150 (MQ=255)
                                                                                                                            ggATTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGACCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGGTGGCGCACAAGTcgcgcg                    >  2:1165379/1‑150 (MQ=255)
                                                                                                                              aTTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGAcc                 >  2:411752/1‑151 (MQ=255)
                                                                                                                              aTTGCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGAcc                 <  1:1407460/151‑1 (MQ=255)
                                                                                                                                 gCGTCGTGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGaa              <  2:1051044/151‑1 (MQ=255)
                                                                                                                                      gtGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATa         <  2:141508/151‑1 (MQ=255)
                                                                                                                                       tGACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATa         >  1:1397393/1‑150 (MQ=255)
                                                                                                                                        gACCACAGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATAc        <  1:447476/150‑1 (MQ=255)
                                                                                                                                            acaGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTTGCGCACAAGTCGCGCGACCGAACGATACCGtt    >  1:750145/1‑150 (MQ=255)
                                                                                                                                             caGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGt  >  2:195465/1‑151 (MQ=255)
                                                                                                                                             caGATGCGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGt  <  2:87714/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAATTTCATCACCTCCAGCGGTTTAGGTACCATGGGTTTTGGTTTACCGGCGGCGGTTGGCGCACAAGTCGCGCGACCGAACGATACCGTTGT  >  NC_000913/3951534‑3951825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: