BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,410,605	C→T	A18V (GCG→GTG)	yjfJ →	PspA/IM30 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,410,605	0	C	T	97.4%	394.9 / ‑2.5	115	A18V (GCG→GTG)	yjfJ	PspA/IM30 family protein
Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (58/53); total (61/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGATAAGCTGAAAGATTTACG  >  NC_000913/4410458‑4410752

atatCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTgg                                                                                                                                                   >  1:856322/1‑150 (MQ=255)

    ccTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGgaaga                                                                                                                                               <  1:541842/150‑1 (MQ=255)

       gCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATcc                                                                                                                                           <  1:840323/151‑1 (MQ=255)

        cTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAAAATc                                                                                                                                            <  1:1062960/149‑1 (MQ=255)

         ttGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCAt                                                                                                                                         >  2:630574/1‑151 (MQ=255)

         ttGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCAt                                                                                                                                         >  2:1234442/1‑151 (MQ=255)

          tGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCa                                                                                                                                          <  2:1037829/149‑1 (MQ=255)

          tGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCAtt                                                                                                                                        >  2:1249696/1‑151 (MQ=255)

              aTAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTgaag                                                                                                                                    <  2:1167510/151‑1 (MQ=255)

                 aCCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGaaa                                                                                                                                 <  1:760999/151‑1 (MQ=255)

                   cTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAAcc                                                                                                                               >  1:1370832/1‑151 (MQ=255)

                     cGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAAccc                                                                                                                              <  2:472919/150‑1 (MQ=255)

                     cGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAAccc                                                                                                                              <  1:621592/150‑1 (MQ=255)

                         ggTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGc                                                                                                                         <  1:474578/151‑1 (MQ=255)

                           tAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGt                                                                                                                       >  2:1140739/1‑151 (MQ=255)

                            aGATAACGCGTCGGATATGGGTGAGCTTACAGAAGAATATTCACGCTAATTACAAGGAGGGGTATTTATGGGAATTTTAAAAAGTTAATTCACGCTGGGGAAATGGTTTATCTCCAAGGGGGAAGAATCCATTGAAGAAAGATAGGGCGt                                                                                                                       >  1:50354/1‑150 (MQ=255)

                            aGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTg                                                                                                                      <  2:571176/151‑1 (MQ=255)

                                   gCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGc                                                                                                               >  2:622488/1‑151 (MQ=255)

                                       tGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTgg                                                                                                            >  2:134559/1‑150 (MQ=255)

                                            cTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGc                                                                                                      >  2:807812/1‑151 (MQ=255)

                                              ggCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGTCGTGCGCATGCTGGAACAGc                                                                                                      <  1:337514/149‑1 (MQ=255)

                                              ggCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGTCGTGCGCATGCTGGAACAGCat                                                                                                    >  1:141376/1‑151 (MQ=255)

                                              ggCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCat                                                                                                    <  1:109962/151‑1 (MQ=255)

                                                 tGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATAtt                                                                                                 >  1:14875/1‑151 (MQ=255)

                                                  gAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATAtt                                                                                                 <  1:339305/150‑1 (MQ=255)

                                                  gAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATAtt                                                                                                 <  2:943988/150‑1 (MQ=255)

                                                   aaaTTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCg                                                                                               >  2:107039/1‑151 (MQ=255)

                                                   aaaTTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCg                                                                                               >  2:1243719/1‑151 (MQ=255)

                                                     aTTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTg                                                                                             <  2:535246/151‑1 (MQ=255)

                                                     aTTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTg                                                                                             <  1:545595/151‑1 (MQ=255)

                                                       tACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGAc                                                                                           >  1:1038670/1‑151 (MQ=255)

                                                         cAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGc                                                                                         >  1:790026/1‑151 (MQ=255)

                                                          agaagaATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCt                                                                                        <  1:631289/151‑1 (MQ=255)

                                                          agaagaATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCt                                                                                        >  1:1158788/1‑151 (MQ=255)

                                                           gaagaaTATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCt                                                                                        >  2:194937/1‑150 (MQ=255)

                                                             agaaTATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTaaa                                                                                     <  2:698877/151‑1 (MQ=255)

                                                                atatTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCt                                                                                  >  2:434842/1‑151 (MQ=255)

                                                                   ttCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGa                                                                                <  1:717991/150‑1 (MQ=255)

                                                                    tCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGaa                                                                               >  1:1116043/1‑150 (MQ=255)

                                                                     cacaCTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACt                                                                             <  2:320348/151‑1 (MQ=255)

                                                                     cacaCTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACt                                                                             <  2:102482/151‑1 (MQ=255)

                                                                        acTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCg                                                                           >  2:695415/1‑150 (MQ=255)

                                                                         cTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGAt                                                                         >  1:280095/1‑151 (MQ=255)

                                                                           aaTTACAAGGACGGGTAATTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGACGAGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATa                                                                        >  1:191599/1‑150 (MQ=255)

                                                                               aCAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGcc                                                                   >  2:1175120/1‑151 (MQ=255)

                                                                               aCAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGc                                                                    <  1:255644/150‑1 (MQ=255)

                                                                                cAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCg                                                                  >  2:974605/1‑151 (MQ=255)

                                                                                 aaGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCgg                                                                 <  1:1353109/151‑1 (MQ=255)

                                                                                 aaGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCgg                                                                 >  2:754238/1‑151 (MQ=255)

                                                                                   ggACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGaa                                                               <  2:462850/151‑1 (MQ=255)

                                                                                   ggACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGaa                                                               <  1:1221989/151‑1 (MQ=255)

                                                                                    gACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGaa                                                               >  2:669497/1‑150 (MQ=255)

                                                                                     aCGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGaaa                                                              >  1:906093/1‑150 (MQ=255)

                                                                                     aCGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGaaa                                                              >  2:621551/1‑150 (MQ=255)

                                                                                      cGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAAt                                                            <  2:1063032/151‑1 (MQ=255)

                                                                                      cGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAAt                                                            >  1:817544/1‑151 (MQ=255)

                                                                                      cGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAAt                                                            <  2:1163652/151‑1 (MQ=255)

                                                                                      cGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAAt                                                            <  2:343770/151‑1 (MQ=255)

                                                                                      cGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAAt                                                            >  2:778008/1‑151 (MQ=255)

                                                                                       gggTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAAtc                                                           >  1:807785/1‑151 (MQ=255)

                                                                                         gTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAAtctc                                                         <  1:697003/151‑1 (MQ=255)

                                                                                         gTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAAtct                                                          <  1:1316263/150‑1 (MQ=255)

                                                                                          tATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTcg                                                        >  2:1114229/1‑151 (MQ=255)

                                                                                          tATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTcg                                                        >  1:1384014/1‑151 (MQ=255)

                                                                                          tATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTcg                                                        >  2:128677/1‑151 (MQ=255)

                                                                                             ttATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGCTAAAGCCGGAAAATCTCGCGt                                                     >  1:1118325/1‑151 (MQ=255)

                                                                                               aTGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTg                                                   <  1:1280051/151‑1 (MQ=255)

                                                                                                 gggAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGAt                                                 >  2:313174/1‑151 (MQ=255)

                                                                                                  ggAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATc                                                >  2:9783/1‑151 (MQ=255)

                                                                                                   gAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATct                                               <  2:459852/151‑1 (MQ=255)

                                                                                                    aaTTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAATCCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATctg                                              <  2:937589/151‑1 (MQ=255)

                                                                                                        ttAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTgg                                          >  2:760197/1‑151 (MQ=255)

                                                                                                        ttAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTgg                                          >  2:193836/1‑151 (MQ=255)

                                                                                                          aaaaaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGAT‑AAGCCGGAAAATCTCGCGTTGATCTGCTGgcg                                        <  1:1048530/150‑1 (MQ=255)

                                                                                                             aaGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCgg                                     >  1:988574/1‑151 (MQ=255)

                                                                                                              aGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCggg                                    <  2:566401/151‑1 (MQ=255)

                                                                                                                tttatttaCGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTg                                  >  1:349258/1‑151 (MQ=255)

                                                                                                                 ttatttaCGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTg                                  >  1:756509/1‑150 (MQ=255)

                                                                                                                  tatttaCGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGACAATCTCGCGTTGATCTGCTGGCGCGGGTGa                                 >  1:574999/1‑150 (MQ=255)

                                                                                                                  tatttaCGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGa                                 <  2:1225570/150‑1 (MQ=255)

                                                                                                                   atttaCGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGaa                                >  1:832935/1‑150 (MQ=255)

                                                                                                                     ttaCGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAAtt                             >  1:157194/1‑151 (MQ=255)

                                                                                                                     ttaCGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAAtt                             >  1:285657/1‑151 (MQ=255)

                                                                                                                        cGCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTa                            >  2:1258066/1‑149 (MQ=255)

                                                                                                                         gCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCCTTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTa                            >  1:1061757/1‑148 (MQ=255)

                                                                                                                         gCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGt                         >  2:238419/1‑151 (MQ=255)

                                                                                                                         gCTGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGt                         <  1:1234360/151‑1 (MQ=255)

                                                                                                                           tGGGGAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTc                        >  1:1002244/1‑150 (MQ=255)

                                                                                                                            ggggAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCAc                      <  2:428334/151‑1 (MQ=255)

                                                                                                                            ggggAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGAGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGGGGGTGAAATTAAGTCa                       <  1:875511/150‑1 (MQ=255)

                                                                                                                              ggAAATCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGa                    <  1:352192/151‑1 (MQ=255)

                                                                                                                               gAAATCGTTTATCTCCCAGGCGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGTATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGAt                   <  2:190491/151‑1 (MQ=255)

                                                                                                                                  aTCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGATa                  >  2:1090174/1‑149 (MQ=255)

                                                                                                                                  aTCGTTTATCTCCCAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGAGAAATTAAGTCACGa                    >  2:544181/1‑147 (MQ=255)

                                                                                                                                    cGTTTATCCCCCAGGTGGAAGAACCCATTGAAGAACCCCAGGCCGTGCACAAGCTTGAGCAGCTTATTCGCGGCGCTAAAGCTGATCTCGATAAAGCCGGAAACTCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGATAAGc               <  1:593754/150‑1 (MQ=255)

                                                                                                                                             cccAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGATAAGCTGAAAGAttt     <  1:534547/151‑1 (MQ=255)

                                                                                                                                              ccAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGATAAGCTGAAAGAttt     >  2:387259/1‑150 (MQ=255)

                                                                                                                                               cAGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGATAAGCTGAAAGATTTAc   <  2:856360/151‑1 (MQ=255)

                                                                                                                                                aGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGATAAGCTGAAAGATTTACg  <  2:610326/151‑1 (MQ=255)

                                                                                                                                                aGGTGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGATAAGCTGAAAGATTTACg  <  2:1050979/151‑1 (MQ=255)

ATATCCTGCTTGAGATAACCTCGCTGGTAGATAACGCATTGGATCTGGCTGAAATTACAGAAGAATATTCACACTAATTACAAGGACGGGTATTTATGGGAATTTTAAAAAGTTTATTTACGCTGGGGAAATCGTTTATCTCCCAGGCGGAAGAATCCATTGAAGAAACCCAGGGCGTGCGCATGCTGGAACAGCATATTCGTGACGCTAAAGCTGAACTCGATAAAGCCGGAAAATCTCGCGTTGATCTGCTGGCGCGGGTGAAATTAAGTCACGATAAGCTGAAAGATTTACG  >  NC_000913/4410458‑4410752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`