Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 757,037 T→C D377D (GAT→GAC sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913757,0370TC95.5% 310.9 / 0.7 90D377D (GAT→GACsdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base C (42/43);  total (43/47)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAG  >  NC_000913/756898‑757184
                                                                                                                                           |                                                                                                                                                   
tGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTcc                                                                                                                                          >  2:863053/1‑151 (MQ=255)
  gAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCgg                                                                                                                                        <  2:1226919/151‑1 (MQ=255)
    gCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCggg                                                                                                                                       >  1:455588/1‑150 (MQ=255)
      tgtCCCGTCCCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAGCCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCggg                                                                                                                                       <  1:1177226/146‑1 (MQ=255)
        tCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGt                                                                                                                                  >  2:508493/1‑151 (MQ=255)
         cccGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGtt                                                                                                                                 <  1:283186/151‑1 (MQ=255)
            gTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGc                                                                                                                              >  2:663334/1‑151 (MQ=255)
            gTACCTTCGCTCACGTCGATCCAATGAAAGAGACGATTCCGGGTACCCAAACCTGCCACTACCTGATGGGCGGCATTCCGAGCAAACTTAGCAATCACGCACTGACCGTGAATGAGAAACGCGAAGACGAGGAAGTTCCGGCAcaactcg                                                                                                                               >  1:1290239/1‑144 (MQ=255)
             tACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGcc                                                                                                                             >  2:302576/1‑151 (MQ=255)
             tACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGcc                                                                                                                             >  2:381460/1‑151 (MQ=255)
              aCCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCg                                                                                                                            >  2:253797/1‑151 (MQ=255)
              aCCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCg                                                                                                                            >  2:398413/1‑151 (MQ=255)
              aCCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCg                                                                                                                            <  1:341217/151‑1 (MQ=255)
                  tCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGtt                                                                                                                          <  2:213528/149‑1 (MQ=255)
                  tCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGtt                                                                                                                          <  2:919458/149‑1 (MQ=255)
                  tCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTg                                                                                                                         <  1:1050684/150‑1 (MQ=255)
                   cGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGt                                                                                                                       <  2:73413/151‑1 (MQ=255)
                      tCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGa                                                                                                                     >  1:166141/1‑150 (MQ=255)
                      tCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGa                                                                                                                     >  2:442489/1‑150 (MQ=255)
                        aCGGCGATTCGGTGAAAGAGCCGATTCCGGTTATCCCATCCTGTCACTACATGATGGGCGGTATTCCGACCAAATTTACCTGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGGTGGTGAAAt                                                                                                                  <  1:831745/151‑1 (MQ=255)
                         cGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATc                                                                                                                 >  1:1107541/1‑151 (MQ=255)
                         cGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATc                                                                                                                 <  1:1123505/151‑1 (MQ=255)
                            cGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCt                                                                                                              <  2:1105850/151‑1 (MQ=255)
                             gATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGACGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGAGAAATCGCtt                                                                                                             >  1:762231/1‑151 (MQ=255)
                             gATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCtt                                                                                                             >  1:811854/1‑151 (MQ=255)
                              aTCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtg                                                                                                            >  1:1139155/1‑151 (MQ=255)
                                ccGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtgt                                                                                                           <  2:581771/150‑1 (MQ=255)
                                 cGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTtgtg                                                                                                          >  1:1283246/1‑150 (MQ=255)
                                  ggTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTgtgt                                                                                                         >  2:486085/1‑150 (MQ=255)
                                   gTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTAt                                                                                                       <  1:212406/151‑1 (MQ=255)
                                    tGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATc                                                                                                      >  1:603190/1‑151 (MQ=255)
                                         gagCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTac                                                                                                 >  1:1126880/1‑151 (MQ=255)
                                         gagCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTac                                                                                                 >  1:882032/1‑151 (MQ=255)
                                          agCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTaca                                                                                                <  1:365419/151‑1 (MQ=255)
                                              gATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGACGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGgc                                                                                            >  1:742795/1‑151 (MQ=255)
                                               aTTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGCCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGgcg                                                                                           >  1:187758/1‑151 (MQ=255)
                                                   cGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTa                                                                                        >  1:354956/1‑150 (MQ=255)
                                                   cGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTa                                                                                        >  1:909716/1‑150 (MQ=255)
                                                     gTTATCCCAGCCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCt                                                                                         <  1:53449/147‑1 (MQ=255)
                                                      ttATCCCAACCTTTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGCCTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAAcc                                                                                     <  1:531404/150‑1 (MQ=255)
                                                      ttATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCg                                                                                    >  2:1260117/1‑151 (MQ=255)
                                                        aTCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGt                                                                                   <  1:459107/150‑1 (MQ=255)
                                                          cccAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGGTACCGGTCTGGCACTGACTGTGAATGAGAAACGCGAAGACGTGGTTGTTGCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGtgt                                                                                 <  1:962693/150‑3 (MQ=255)
                                                           ccAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTgg                                                                               <  2:195710/151‑1 (MQ=255)
                                                             aaCCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGggc                                                                             <  1:334402/151‑1 (MQ=255)
                                                                  gTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCa                                                                         <  2:67558/150‑1 (MQ=255)
                                                                  gTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCa                                                                         <  1:221814/150‑1 (MQ=255)
                                                                    cACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACt                                                                      <  2:374775/151‑1 (MQ=255)
                                                                    cACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACt                                                                      <  1:1394112/151‑1 (MQ=255)
                                                                    cACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACt                                                                      <  2:41014/151‑1 (MQ=255)
                                                                      cTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCg                                                                    >  1:829069/1‑151 (MQ=255)
                                                                        aCATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCgct                                                                  <  2:260664/151‑1 (MQ=255)
                                                                        aCATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCgct                                                                  >  1:545044/1‑151 (MQ=255)
                                                                         cATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCgctg                                                                 <  2:98149/151‑1 (MQ=255)
                                                                         cATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCgctg                                                                 <  1:403782/151‑1 (MQ=255)
                                                                         cATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCgctg                                                                 >  1:1145775/1‑151 (MQ=255)
                                                                          atgatgGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCgctg                                                                 >  1:752741/1‑150 (MQ=255)
                                                                            gatgGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGctgctg                                                              <  2:1408635/151‑1 (MQ=255)
                                                                                    gTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCtggt                                                      >  2:1279646/1‑151 (MQ=255)
                                                                                          cGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCtt                                                >  2:799279/1‑151 (MQ=255)
                                                                                          cGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCtt                                                >  1:318573/1‑151 (MQ=255)
                                                                                           gACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCttt                                               <  2:1169048/151‑1 (MQ=255)
                                                                                           gACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCttt                                               <  2:297905/151‑1 (MQ=255)
                                                                                             ccAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTgg                                             <  2:1276188/151‑1 (MQ=255)
                                                                                             ccAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTgg                                             <  2:1413312/151‑1 (MQ=255)
                                                                                                 aGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTcgc                                         <  1:275135/151‑1 (MQ=255)
                                                                                                   ttACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTcgcg                                        >  1:1111485/1‑150 (MQ=255)
                                                                                                    tACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTcgcgcg                                      >  2:96889/1‑151 (MQ=255)
                                                                                                     aCCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTcgcgc                                       <  2:808535/149‑1 (MQ=255)
                                                                                                     aCCGGTCAGGCACTGACTGGGAATGAGAAAGGCGAACAAGTGGTTGTTCCGGGACTCGTTGCCGTTGGTGAAATCGCTTGTGTAACGGTACACGGCGCTAACCGTCTGGGCCGCAACGCGCTGCTGGACCTGATGGTCTTTGGTcc                                          <  1:157856/146‑2 (MQ=255)
                                                                                                       cGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCa                                   <  2:1145849/151‑1 (MQ=255)
                                                                                                         gTCAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAgg                                 >  1:919510/1‑151 (MQ=255)
                                                                                                           cAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtc                               <  2:941260/151‑1 (MQ=255)
                                                                                                           cAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtc                               <  2:318971/151‑1 (MQ=255)
                                                                                                           cAGGCACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtc                               >  2:1033573/1‑151 (MQ=255)
                                                                                                               cACTGACTGTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTGCACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgca                           <  1:186627/151‑1 (MQ=255)
                                                                                                                actgactgTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgcat                          >  2:639843/1‑151 (MQ=255)
                                                                                                                 ctgactgTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgcat                          >  2:381454/1‑150 (MQ=255)
                                                                                                                   gactgTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgcatctg                       <  1:663302/151‑1 (MQ=255)
                                                                                                                   gactgTGAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgcatctg                       >  2:5823/1‑151 (MQ=255)
                                                                                                                        tgAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgcatctgca                     >  2:136907/1‑148 (MQ=255)
                                                                                                                        tgAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAag                   <  2:352215/150‑1 (MQ=255)
                                                                                                                         gAATGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCACCTCGCTGCTGGACCTGGTGGTCTTTGGTCGCTCGGCAGGTCTGCATCTGCAaga                  <  1:195626/150‑1 (MQ=255)
                                                                                                                            tGAGAAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTc               >  1:546146/1‑150 (MQ=255)
                                                                                                                             gagaAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCt              <  2:1159368/150‑1 (MQ=255)
                                                                                                                              agaAAGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTa             <  1:715959/150‑1 (MQ=255)
                                                                                                                                aaaGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGGCTGCATCTGCAAGAGTCTATCg          >  1:1108428/1‑151 (MQ=255)
                                                                                                                                 aaGGCGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGc         >  2:814729/1‑151 (MQ=255)
                                                                                                                                     cGAAGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAg     <  2:456030/151‑1 (MQ=255)
                                                                                                                                        aGACGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAg  <  1:858925/151‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
TGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAG  >  NC_000913/756898‑757184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: