Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 940,856 A→G intergenic (+136/‑103) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913940,8560AG97.8% 329.9 / ‑3.8 90intergenic (+136/‑103)serS/dmsAseryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (61/26);  total (62/28)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTATTGGCTGCTGAGGTG  >  NC_000913/940708‑941000
                                                                                                                                                    |                                                                                                                                                
atatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAgtt                                                                                                                                                >  2:364398/1‑151 (MQ=255)
 tatatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAgtt                                                                                                                                                >  2:764066/1‑150 (MQ=255)
  atatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAgttat                                                                                                                                              >  2:533857/1‑151 (MQ=255)
   tatTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAgttatt                                                                                                                                             >  2:620534/1‑151 (MQ=255)
    atTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTc                                                                                                                                            >  2:148013/1‑151 (MQ=255)
     ttGGCTAATACCCAATTTTTCTTAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATTTACCTATCTCTTTGATTTTCAAGTTATTCg                                                                                                                                           >  1:121215/1‑151 (MQ=255)
      tGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCg                                                                                                                                           <  2:1267763/150‑1 (MQ=255)
       ggCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGAt                                                                                                                                         <  2:1368212/151‑1 (MQ=255)
           aaTACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGta                                                                                                                                      <  2:1424787/150‑1 (MQ=255)
            aTACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGtat                                                                                                                                     >  2:1032337/1‑150 (MQ=255)
             tACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGAATAc                                                                                                                                   >  2:550659/1‑151 (MQ=255)
                ccAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAg                                                                                                                                <  2:1261648/151‑1 (MQ=255)
                  aaTTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGcc                                                                                                                              <  2:978240/151‑1 (MQ=255)
                       ttCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCtata                                                                                                                          <  2:629742/150‑1 (MQ=255)
                        tCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTTCCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCtatat                                                                                                                         <  1:506328/150‑1 (MQ=255)
                        tCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCtatat                                                                                                                         >  1:690749/1‑150 (MQ=255)
                        tCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCtatat                                                                                                                         >  2:499447/1‑150 (MQ=255)
                        tCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCtatat                                                                                                                         >  2:1116831/1‑150 (MQ=255)
                         cTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCtatat                                                                                                                         >  2:1018780/1‑149 (MQ=255)
                           gAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGc                                                                                                                      <  2:211914/150‑1 (MQ=255)
                             aTCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCTTTTGATACCGAACAATAATTCCTCCTCACTTACCCATAATTCTGCTTTCGAAGGAAAATCTACCTATCTCTGTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGa                                                                                                                    <  1:1248241/150‑1 (MQ=255)
                             aTCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGa                                                                                                                    >  1:393838/1‑150 (MQ=255)
                                 aaaaaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAATTTATTCGATGTATACAAGCCTATATAGCGAACt                                                                                                                 <  1:1170285/149‑1 (MQ=255)
                                 aaaaaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGc                                                                                                               >  2:247475/1‑151 (MQ=255)
                                  aaaaGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCt                                                                                                              >  1:977326/1‑151 (MQ=255)
                                    aaGCGACTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAACGAACTGCt                                                                                                              >  1:1119853/1‑149 (MQ=255)
                                     aGCGCCTGCGGGCGCTTTTTTTATCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGACGTATACAAGCCTATATAGCGAACTGCtat                                                                                                            >  2:780096/1‑150 (MQ=255)
                                      gcgcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCtat                                                                                                            >  2:327402/1‑149 (MQ=255)
                                      gcgcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAg                                                                                                          <  2:43860/151‑1 (MQ=255)
                                       cgacTGCGGGCGCTTTTTTTGTCTCCCTTCGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCtat                                                                                                            >  1:596680/4‑148 (MQ=255)
                                        gcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTCCCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGaa                                                                                                        <  2:390016/151‑1 (MQ=255)
                                        gcCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGaa                                                                                                        <  2:353073/151‑1 (MQ=255)
                                         ccTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGaaa                                                                                                       <  2:358357/151‑1 (MQ=255)
                                           tGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaat                                                                                                      >  1:160441/1‑150 (MQ=255)
                                             cGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAaataat                                                                                                   >  2:409069/1‑151 (MQ=255)
                                               ggCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAAtt                                                                                                  >  1:1146976/1‑150 (MQ=255)
                                                    tttttttGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTAcaca                                                                                             >  1:771227/1‑150 (MQ=255)
                                                    tttttttGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTAcaca                                                                                             >  1:84665/1‑150 (MQ=255)
                                                     ttttttGTCTCCCTTTGATCCCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTAcaca                                                                                             <  1:1100768/149‑1 (MQ=255)
                                                     ttttttGTCGCCCTTTGATACCGCACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGCGAAAATCTACCGATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGGGCTGCTATAGAAATAATTAtaca                                                                                             >  1:1173544/1‑149 (MQ=255)
                                                          tGTCTCCCTTTGATACCGAACAATAATTACCCCTCACTTACACTTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATATATACAAGCCTATATCGCGAACTGCTATAGAAATAATTACACAATAc                                                                                         <  1:1412652/148‑1 (MQ=255)
                                                           gTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGt                                                                                      >  1:401634/1‑150 (MQ=255)
                                                            tctcCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGtt                                                                                     <  1:508688/150‑1 (MQ=255)
                                                            tctcCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGtt                                                                                     <  1:1394543/150‑1 (MQ=255)
                                                              tcCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGt                                                                                  >  1:325726/1‑151 (MQ=255)
                                                               cccTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTTCCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGttt                                                                                    <  1:1391301/148‑1 (MQ=255)
                                                               cccTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGtt                                                                                 >  1:128044/1‑151 (MQ=255)
                                                                ccTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGtt                                                                                 >  1:375848/1‑150 (MQ=255)
                                                                   ttGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTAc                                                                               <  1:579435/149‑1 (MQ=255)
                                                                      aTACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTgg                                                                            <  2:1095173/149‑1 (MQ=255)
                                                                      aTACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGa                                                                           >  2:603549/1‑150 (MQ=255)
                                                                          cGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGaatca                                                                       >  2:196729/1‑150 (MQ=255)
                                                                           gatcAATAATAACTCCTGACTGGAAAGGAAGACTAATTTCGAGGGAAGCTCTACCTATCTATTAGATATACAAATTATTCGATGTATACAAGCCTATATAGCGAACTGATATAGAAATAATTACACAATACGGTTTGCTACTGGaatcaat                                                                     >  1:1048943/4‑151 (MQ=255)
                                                                           gAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGaatcaat                                                                     >  1:245666/1‑151 (MQ=255)
                                                                            aaCAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGaatcaatc                                                                    >  2:274670/1‑151 (MQ=255)
                                                                              cAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGCATACAAGCCTATATAGAGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCg                                                                   >  1:1029277/1‑150 (MQ=255)
                                                                               aataatTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTg                                                                 <  2:1384321/151‑1 (MQ=255)
                                                                                  aatTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAg                                                               >  1:1099255/1‑150 (MQ=255)
                                                                                  aatTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAg                                                               <  1:818663/150‑1 (MQ=255)
                                                                                  aatTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGc                                                              >  2:1182415/1‑151 (MQ=255)
                                                                                  aatTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGc                                                              >  2:180747/1‑151 (MQ=255)
                                                                                    ttACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCa                                                             >  2:333198/1‑150 (MQ=255)
                                                                                     tACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCa                                                             >  1:489883/1‑149 (MQ=255)
                                                                                              cTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTtgagtg                                                   >  2:1407298/1‑150 (MQ=255)
                                                                                                  cacGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCa                                              <  1:577937/151‑1 (MQ=255)
                                                                                                   acGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCAt                                             >  2:164953/1‑151 (MQ=255)
                                                                                                       aaTACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATg                                         <  2:496681/151‑1 (MQ=255)
                                                                                                         tactacTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGa                                        >  2:627773/1‑150 (MQ=255)
                                                                                                         tactacTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGa                                        >  1:1279072/1‑150 (MQ=255)
                                                                                                             actTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAAc                                    <  2:545665/150‑1 (MQ=255)
                                                                                                             actTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACg                                   >  1:1418274/1‑151 (MQ=255)
                                                                                                               tttCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGa                                  >  1:246482/1‑150 (MQ=255)
                                                                                                                  cGAGTGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAAt                              >  1:94813/1‑151 (MQ=255)
                                                                                                                    aGTGAGAATCTACCTAACTCTTTGTTTTTCACGGTATTCGCTGTATACAAGACTATATAGCGAAATCCTATAGAAATAATTACACAATACGGTTTGTTACTGGAACCTATCGTGAGCAATCATGAGTGATCCATTATGAAACCGAAAATc                             >  1:980361/1‑150 (MQ=255)
                                                                                                                      tGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCt                          >  2:267351/1‑151 (MQ=255)
                                                                                                                      tGAAAATCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTCGAGTGAGCCATTATGAAAACGAAAATCCCt                          >  2:785855/1‑151 (MQ=255)
                                                                                                                        aaaaTCTACCTATCTCTTTGATTTTCAAGCTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCAATTATGAAAACGAAAATCCCTg                         >  1:931542/1‑150 (MQ=255)
                                                                                                                         aaaTCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGAt                       <  2:1418790/151‑1 (MQ=255)
                                                                                                                          aaTCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATg                      >  1:1420637/1‑151 (MQ=255)
                                                                                                                           aTCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGc                     >  1:428367/1‑151 (MQ=255)
                                                                                                                            tCTACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCg                    >  1:391597/1‑151 (MQ=255)
                                                                                                                              tACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACGATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCgg                   >  1:401773/1‑150 (MQ=255)
                                                                                                                              tACCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGt                  >  1:203035/1‑151 (MQ=255)
                                                                                                                               aCCTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGt                  >  2:886450/1‑150 (MQ=255)
                                                                                                                                 cTATCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTAtt               >  2:185176/1‑151 (MQ=255)
                                                                                                                                   aTCTCTTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTATTgg             >  1:1128663/1‑151 (MQ=255)
                                                                                                                                     ctctTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTATTGgct           >  2:255044/1‑151 (MQ=255)
                                                                                                                                       ctTTGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTATTGgctgc         >  2:96529/1‑151 (MQ=255)
                                                                                                                                        ttttATTTTCAAGTCATTCGAAGTATACAAGCCTATATAGCGATATGCTATAGCAATAATTAAACAATAAGATATCTTACTGGAATCAATCGTGAGCAAACTTGAGTGATCCATTATGAAAACGATACTCCCTGATGTGGAATTGgctgct        >  1:331176/1‑151 (MQ=255)
                                                                                                                                        tttGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTATTGgctgc         >  2:1105294/1‑150 (MQ=255)
                                                                                                                                         ttGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTATTGGctgctg       >  2:360081/1‑151 (MQ=255)
                                                                                                                                          tGATTTTCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTATTGGctgctg       <  2:1208507/150‑1 (MQ=255)
                                                                                                                                              tttCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTATTGGCTGCTGAGGTg  >  1:387847/1‑151 (MQ=255)
                                                                                                                                               ttCAAGTTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTATTGGCTGCTGAGGTg  >  2:491076/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ATATATTGGCTAATACCCAATTTTTCTGAATCTAAAAAGCGCCTGCGGGCGCTTTTTTTGTCTCCCTTTGATACCGAACAATAATTACTCCTCACTTACACGTAATACTACTTTCGAGTGAAAATCTACCTATCTCTTTGATTTTCAAATTATTCGATGTATACAAGCCTATATAGCGAACTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGAGCAAGCTTGAGTGAGCCATTATGAAAACGAAAATCCCTGATGCGGTATTGGCTGCTGAGGTG  >  NC_000913/940708‑941000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: