Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,198,201 C→T E13K (GAA→AAA)  ymfE ← e14 prophage; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,198,2010CT97.7% 311.8 / ‑1.2 88E13K (GAA→AAA) ymfEe14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (46/40);  total (46/42)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

CCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCG  >  NC_000913/1198054‑1198348
                                                                                                                                                   |                                                                                                                                                   
cccAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTAtt                                                                                                                                                  >  1:476030/1‑151 (MQ=255)
 ccAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTttatta                                                                                                                                                 <  1:944547/151‑1 (MQ=255)
  cAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTtattat                                                                                                                                                <  2:924955/151‑1 (MQ=255)
   aaTTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATGCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATtata                                                                                                                                               <  2:1147328/151‑1 (MQ=255)
    aTTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATtata                                                                                                                                               >  2:41580/1‑150 (MQ=255)
      taataaAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAt                                                                                                                                             <  1:653279/150‑1 (MQ=255)
       aataaAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAttt                                                                                                                                           >  2:184955/1‑151 (MQ=255)
       aataaAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAtt                                                                                                                                            <  1:837729/150‑1 (MQ=255)
       aataaAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAAtt                                                                                                                                            <  2:321407/150‑1 (MQ=255)
           aaGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTgg                                                                                                                                       >  1:1392664/1‑151 (MQ=255)
           aaGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTgg                                                                                                                                       <  1:594916/151‑1 (MQ=255)
             gCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTgggg                                                                                                                                     >  1:5841/1‑151 (MQ=255)
                  cATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTgggggg                                                                                                                                   <  2:219872/148‑1 (MQ=255)
                      aTCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAAc                                                                                                                            >  2:554874/1‑151 (MQ=255)
                          ttAATAGGTGGGACATACTTGGTTGGTAATGTTATTACAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACatat                                                                                                                        >  2:1342649/1‑151 (MQ=255)
                          ttAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGaa                                                                                                                             <  1:1303633/146‑1 (MQ=255)
                             aTAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACAtattat                                                                                                                     >  1:594986/1‑151 (MQ=255)
                                  tGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCAcc                                                                                                                >  2:111989/1‑151 (MQ=255)
                                  tGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCAcc                                                                                                                >  2:598661/1‑151 (MQ=255)
                                   gggACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCt                                                                                                               >  2:694631/1‑151 (MQ=255)
                                   gggACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCt                                                                                                               >  2:1099794/1‑151 (MQ=255)
                                        aTACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTGGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTc                                                                                                          >  1:686552/1‑151 (MQ=255)
                                          aCTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCAc                                                                                                        <  2:293270/151‑1 (MQ=255)
                                            ttggttggTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCAc                                                                                                        >  1:278287/1‑149 (MQ=255)
                                             tggttggtAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGc                                                                                                      <  1:1344758/150‑1 (MQ=255)
                                              ggttggtAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCtt                                                                                                    <  2:432197/151‑1 (MQ=255)
                                              ggttggtAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCt                                                                                                     <  2:888307/150‑1 (MQ=255)
                                                 tggtAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTa                                                                                                  <  1:918512/150‑1 (MQ=255)
                                                 tggtAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTa                                                                                                  <  2:1286686/150‑1 (MQ=255)
                                                 tggtAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTa                                                                                                  <  1:797738/150‑1 (MQ=255)
                                                     aaTGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGAt                                                                                              >  2:1229958/1‑150 (MQ=255)
                                                     aaTGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGAt                                                                                              <  2:1201344/150‑1 (MQ=255)
                                                     aaTGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATa                                                                                             <  2:901358/151‑1 (MQ=255)
                                                      aTGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAg                                                                                            <  2:832443/151‑1 (MQ=255)
                                                      aTGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAg                                                                                            <  2:124813/151‑1 (MQ=255)
                                                        gTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAgt                                                                                           <  2:55604/150‑1 (MQ=255)
                                                         ttattaAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAgtgt                                                                                         >  1:1166135/1‑151 (MQ=255)
                                                          tattaAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAgtgt                                                                                         >  2:1206280/1‑150 (MQ=255)
                                                          tattaAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTAACGCTTTAGGATAgtgt                                                                                         >  2:454435/1‑150 (MQ=255)
                                                           attaAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTa                                                                                        <  1:446244/150‑1 (MQ=255)
                                                           attaAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTa                                                                                        <  1:711257/150‑1 (MQ=255)
                                                             taAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAAt                                                                                      <  2:280514/150‑1 (MQ=255)
                                                             taAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATg                                                                                     >  1:216981/1‑151 (MQ=255)
                                                              aaaGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATgg                                                                                    >  2:1190596/1‑151 (MQ=255)
                                                                aGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATgg                                                                                    >  2:1248076/1‑149 (MQ=255)
                                                                aGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGt                                                                                   >  2:1211681/1‑150 (MQ=255)
                                                                  ggATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAat                                                                                <  1:802561/151‑1 (MQ=255)
                                                                  ggATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAat                                                                                >  2:161017/1‑151 (MQ=255)
                                                                      gTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATAttt                                                                            <  2:125397/151‑1 (MQ=255)
                                                                         ttCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaat                                                                         >  1:1065910/1‑151 (MQ=255)
                                                                          tCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaat                                                                         <  2:900349/150‑1 (MQ=255)
                                                                           cGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATttaatt                                                                        <  2:1398972/150‑1 (MQ=255)
                                                                              aaaTAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAg                                                                     <  1:1304296/150‑1 (MQ=255)
                                                                              aaaTAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAg                                                                     <  1:559009/150‑1 (MQ=255)
                                                                               aaTAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAgtg                                                                   >  1:1071172/1‑151 (MQ=255)
                                                                                      aGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGTATAGTGTAATGGTAATATTTAATTAAGTGTCAtt                                                              <  1:388875/149‑1 (MQ=255)
                                                                                      aGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCAtt                                                              <  2:885349/149‑1 (MQ=255)
                                                                                          gTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATAc                                                        >  1:584890/1‑151 (MQ=255)
                                                                                            aTGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACtt                                                      <  1:818104/151‑1 (MQ=255)
                                                                                                aaTATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCa                                                  <  2:728150/151‑1 (MQ=255)
                                                                                                 atgtagtAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAGCATTTTATTATAATTTGTTGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATAGtt                                                      <  1:565020/140‑1 (MQ=255)
                                                                                                 atatatTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTc                                                   >  2:608700/1‑149 (MQ=255)
                                                                                                    tatTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGt                                                >  1:451274/1‑149 (MQ=255)
                                                                                                    tatTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTAGTATAATTTTTGGGGGGTTCGAACATATTATTCTCCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTa                                               <  2:888401/150‑1 (MQ=255)
                                                                                                    aatTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAg                                                 >  1:865480/2‑148 (MQ=255)
                                                                                                     atTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAgg                                             >  1:285267/1‑151 (MQ=255)
                                                                                                     atTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAg                                              <  1:1049017/150‑1 (MQ=255)
                                                                                                      ttAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgt                                            >  2:1335892/1‑151 (MQ=255)
                                                                                                        aGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgtta                                          <  1:948076/151‑1 (MQ=255)
                                                                                                         gCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgttag                                         <  2:182533/151‑1 (MQ=255)
                                                                                                          cGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCATGCTTTAGGATAGTGTATTGGTACTATTTAATTAAGTGTCATTATATACTTTTCAGTAGgttagt                                        >  1:970378/1‑151 (MQ=255)
                                                                                                          cGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgttagt                                        >  2:222709/1‑151 (MQ=255)
                                                                                                           gTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGgttagtt                                       <  1:274651/151‑1 (MQ=255)
                                                                                                             aTTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACGTTTTATTATAATTTTTGGTGGGTGCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAACATTTAATTAAGTGTCATTATATGCTTTTCAATAGtttagtta                                      <  1:165353/150‑1 (MQ=255)
                                                                                                             aTTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTAc                                     >  2:750325/1‑151 (MQ=255)
                                                                                                               ttAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACa                                    <  2:739476/150‑1 (MQ=255)
                                                                                                                tAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAAt                                  >  1:1354035/1‑151 (MQ=255)
                                                                                                                 aaGAAAGTTGCTTTATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAAtt                                 >  2:13683/1‑151 (MQ=255)
                                                                                                                             ttATGAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGt                     >  1:345440/1‑151 (MQ=255)
                                                                                                                                 gAAGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCa                  <  2:1102817/150‑1 (MQ=255)
                                                                                                                                  aaGTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAg                 >  2:1247205/1‑150 (MQ=255)
                                                                                                                                    gTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCt              >  2:1029463/1‑151 (MQ=255)
                                                                                                                                    gTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCt              >  1:1381384/1‑151 (MQ=255)
                                                                                                                                    gTTTAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCt              >  2:1125188/1‑151 (MQ=255)
                                                                                                                                      ttAGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTg             >  1:715507/1‑150 (MQ=255)
                                                                                                                                        aGGCAACATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGAcc          >  1:684428/1‑151 (MQ=255)
                                                                                                                                             aCATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCt     >  2:214893/1‑151 (MQ=255)
                                                                                                                                              cATTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCtt    >  2:886687/1‑151 (MQ=255)
                                                                                                                                               aTTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTc   <  1:357032/151‑1 (MQ=255)
                                                                                                                                               aTTTTATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTc   >  2:253165/1‑151 (MQ=255)
                                                                                                                                                ttttATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCg  >  2:1138001/1‑151 (MQ=255)
                                                                                                                                                ttttATGATAATTTTTGGGGGGATCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATATGTTCAGGCTGACCTAGATTc   >  1:1390341/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTTTAGGCAACATTTCATTATAATTTTTGGGGGGTTCGAACATATTATTCACCTGAGTCACGCTTTAGGATAGTGTAATGGTAATATTTAATTAAGTGTCATTATATACTTTTCAGTAGGTTAGTTACAATTTTTTGTATCTGTTCAGGCTGACCTAGCTTCG  >  NC_000913/1198054‑1198348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: