Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9180AC43.5% 99.4 / 12.8 71intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base A (32/7);  new base C (0/30);  total (32/39)
Fisher's exact test for biased strand distribution p-value = 3.31e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGG  >  NC_000913/2001769‑2002065
                                                                                                                                                       |                                                                                                                                                     
aGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAAGTCCAGGCAGAAAAAAAc                                                                                                                                                     <  1:795760/151‑1 (MQ=255)
 gCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcc                                                                                                                                                    >  1:961564/1‑151 (MQ=255)
   ggTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcccc                                                                                                                                                 >  2:1151354/1‑151 (MQ=255)
   ggTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcccc                                                                                                                                                 >  1:1220083/1‑151 (MQ=255)
   ggTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAcccc                                                                                                                                                 >  1:258665/1‑151 (MQ=255)
      ataGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCccgcc                                                                                                                                              >  1:218686/1‑151 (MQ=255)
            tGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTgg                                                                                                                                         >  2:660032/1‑150 (MQ=255)
                   aCGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGa                                                                                                                                   >  1:1135843/1‑149 (MQ=255)
                    cGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAg                                                                                                                                 >  1:339592/1‑150 (MQ=255)
                      aTAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCa                                                                                                                               >  1:986017/1‑150 (MQ=255)
                       tAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACg                                                                                                                             >  2:1157708/1‑151 (MQ=255)
                        aaccgccccTGGTTTATATGAGTTATGGGCATGATTATCCTTTTCTGCGGGGTTTTTAATGGGACGATTGGGGGGTAAAATGGGGGTTTATTTGGGGGTTAAAGGAAATTTCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGc                                                                                                                                <  1:831357/141‑1 (MQ=255)
                        aaaCAGCCCTGCTTTATATGATTTTTCGGCATGATTTTCCGTTTCTGCAGGTTTTTTAATGGGAGGTTTAGGGGGTAAAATGGGGGTTTATTGGGGGGTTACAGGTAAATTCCAGGAAAAAAAAAACCCCGCCGGTGGCGGGGAAGc                                                                                                                                <  2:318237/147‑1 (MQ=255)
                        aaaCAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCAc                                                                                                                              >  1:741383/1‑149 (MQ=255)
                            cgccctgtttattatGAGTTATCGGCATGATAGTCCGTTTCTGGGGGGTTTTAAATCGGACTATTAGTGGGGTAAATGGGGGGTTATTTGGGGGTTGCAGGAAACTTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCt                                                                                                                        <  2:1102688/139‑1 (MQ=255)
                                  gCGTTATATGTGTTAGCGGCATGATTATCCTTTTCTGGGGGGTTTTAAAGGGGACGATTAGGGGGTAAAGTGAGGGTTTATTGGGGGGTTACAGTTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCt                                                                                                                        <  2:470543/145‑1 (MQ=255)
                                    tttATATGATTGAGCGGATTGTTTATCCTTTTCTGCGTGGTTTTTAACCGGACAATGAGGGGGAAAAAGGAGGGGTTATTTGGGGGTTACAGATAATTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                                 <  1:556994/149‑1 (MQ=255)
                                     taatatGGTTTATCGGCTTGATTTTCCTTTTCGGGGGGGTTTTAAATGGGACAATTAGGGGGAAAAAGGGGGGTTTTTTTGGGGGTTGGGGGAAAATACAAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaa                                                                                                                   <  2:529704/145‑1 (MQ=255)
                                      tataTGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGGGGGGTTATTTGGGGGATACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                               >  2:138015/1‑150 (MQ=255)
                                        tatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGGTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcgc                                                                                                            >  1:583000/1‑151 (MQ=255)
                                             gTTATCGGCATAATTATCCGTTTCTGAAGGTTTTTTAATCGGACGATTGGGGGGTAAAAGGGGGGGTTTTTTGGGGGTAACAGGAAATTCCCAGAAAGAAAAAAGCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTa                                                                                                        <  1:335576/150‑1 (MQ=255)
                                             gTTACCGGCATGATTACCCTTTTCTGCAGGTTTTTTAATCGGACGATTAGTGGGTAAAAGGAGGGTTTTTTTGGGGTTAACAGTAAAATCCCAGAAAAAAAAACACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                         <  1:208067/149‑1 (MQ=255)
                                              ttacgggCATGTTTTTCCTTTTCTGCAGGTTTTTTAAGCGGAGGTTTGGTGGGGGAAATGGGGGTTTTTTGGGGGTTTACGGGAAAATCCCGGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                       <  2:893463/145‑1 (MQ=255)
                                                aTCGGCATGATTATCCTTTTCTGCAGGTTTTTTAATCGGACTATTAGTGGGGGAAAGGAGGGTTTATTTGGGGGTTACAGGTAAATTCCAGGAAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGt                                                                                                     <  2:1134485/150‑1 (MQ=255)
                                                  cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGGTg                                                                                                   >  1:576268/1‑150 (MQ=255)
                                                  cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGAGGGTGAAATGAGGGGTTCTTTGGGGGCTACAGGTAAATTCCAGGCAGAAAAAAACCACGCCGGCGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTctgctgt                                                                                                  >  2:814953/1‑150 (MQ=255)
                                                   ggCATGATTATCCGTTTCGGCGGGTTTTTTAATCGGAGTATTAGGGGGTAAAATGAGGGGTTTTTTGGGGGTAACAGGAAAATCCCAGGAAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTg                                                                                                   <  1:927804/149‑1 (MQ=255)
                                                    gCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGcc                                                                                                 >  1:1162434/1‑150 (MQ=255)
                                                       tGATTCTCGGTTTGTGCGGGTTTTTTAACGGGCCAATTGGGGGGGAAAAAGGGGGGTTGTTGGGGGGTTACAGAAAAATTCCTGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCg                                                                                                <  2:488195/148‑1 (MQ=255)
                                                          ttATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                                          >  2:175049/1‑151 (MQ=255)
                                                                 tttCGGCAGGTTTTTTAATGGGACGATTAGGGGGAAAAAGGAGGGTTTATTGGGGGGTTAAAGTTAAATCCCGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggcg                                                                                       <  2:756339/147‑1 (MQ=255)
                                                                  ttCTGCAGGTTTTTAAAGCGGACGATTGGGGGGTGAAATGAGGGGTTATTTGGGGGTTAAAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaaa                                                                                   <  1:221522/150‑1 (MQ=255)
                                                                     tGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGcc                                                                               >  1:3177/1‑151 (MQ=255)
                                                                     tGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTCAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCt                                                                              >  1:816270/1‑151 (MQ=255)
                                                                          gttttttAATCGGAGGATTGGTGGGTGAAATGGGGGTTTTTTTGGGGGTAAAAGTAAAATCCCAGGAAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                                 <  1:636740/142‑1 (MQ=255)
                                                                          ggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                          >  2:915130/1‑151 (MQ=255)
                                                                            tttttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                        >  2:350172/1‑151 (MQ=255)
                                                                            tttttAAGCGGACGATTGGGGGGTAAAAGGGGGGGTTTTTTGGGGGTTACAGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                          <  1:152263/149‑1 (MQ=255)
                                                                            ttttaAAACGGACGATTAGTGGGGGAAATGAGGGTTTATTTGGGGTTTACGGGAAAATACGAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                         <  2:43951/150‑1 (MQ=255)
                                                                              tttatggggACGATTGGGGGGGAAAAGGGGGGTTTTTTTGGGGTTTACAGGAAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                       <  1:140002/143‑1 (MQ=25)
                                                                              tttAATCGGACGATTAGTGGGTAAAATGGGGGTTTATTGGGGGTTTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                       <  2:35121/150‑1 (MQ=38)
                                                                              tttAATCGGACGATTAGGGGGGAAAATGGGGGTTTTTTTGGGGGTAACAGTTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                       <  1:487243/150‑1 (MQ=37)
                                                                               ttatccGGACGTTTAGGGGGAAAAATGAGGGTTTTTGGGGGGTTAACAGTTAATTCCCGGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                      <  2:468180/145‑1 (MQ=25)
                                                                                       aCGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                              >  2:721749/1‑150 (MQ=255)
                                                                                        cGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                            >  2:596052/1‑151 (MQ=255)
                                                                                         gATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                           >  1:708627/1‑151 (MQ=255)
                                                                                           ttggggggTAAAAGGGGGGTTTATTGGGGGTTTACAGATAAATCCCAGACAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                            <  2:888695/143‑1 (MQ=25)
                                                                                           ttggggggTAAAAGGAGGGTTTATTTGGGGGTTACAGGTAAATTCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                            <  2:54025/143‑1 (MQ=37)
                                                                                              gggggTAAAATGGGGGTTTTTTTGGGGTTTAAAGGTAAATTCAAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgtg                                                         <  1:467061/146‑1 (MQ=37)
                                                                                              gTGGGTGAAATGGGGGTTTTTTGGGGGGTTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgtg                                                         <  2:923696/148‑1 (MQ=38)
                                                                                               tGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaa                                                      >  1:505539/1‑150 (MQ=255)
                                                                                                  gggAAAAGGGGGGTTTTTTGGGGGTTTAAAGGAAATTCCAGGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt                                                   <  2:1021886/149‑1 (MQ=25)
                                                                                                   tGAAATTAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                                  >  2:1108072/1‑150 (MQ=255)
                                                                                                    gAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCa                                                 >  1:616871/1‑150 (MQ=255)
                                                                                                    aaaaggggggtTTATTTGGGGGTAGAAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTc                                                  <  2:860363/142‑1 (MQ=25)
                                                                                                     aaaTGGGGGTTTATTGGGGGTTTACAGGAAAATCCCGGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCa                                                 <  2:447412/149‑1 (MQ=37)
                                                                                                       aTGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                              >  2:1038973/1‑150 (MQ=255)
                                                                                                        tGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaa                                             >  1:897302/1‑150 (MQ=255)
                                                                                                           ggggTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                                          >  1:1129971/1‑150 (MQ=255)
                                                                                                              tttATTGGGGGTTTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                       <  2:20775/149‑1 (MQ=25)
                                                                                                               ttttttGGGGGTTACAGATAAATCCCAGACAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                                      <  1:333946/147‑1 (MQ=37)
                                                                                                               ttATTTGGGGGTTAAAGTAAAACTCCGGGTAGAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                                      <  2:717263/150‑1 (MQ=25)
                                                                                                                  tttGGGGTTAACAGTAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                                   <  1:563972/150‑1 (MQ=25)
                                                                                                                  tttGGGGGTTAAAGTTAATTCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt                                     <  1:626178/148‑1 (MQ=37)
                                                                                                                  tgtGGGGGTTAAAGTTAAATCCCAGACAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                    <  2:933287/147‑1 (MQ=37)
                                                                                                                   ttGGGGTTAACGGGAAATTTCGAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                                  <  1:1216858/150‑1 (MQ=25)
                                                                                                                                   taactccaagaaaGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                         <  1:578772/133‑1 (MQ=255)
                                                                                                                                    aactcccAGGGAAAAAAATGCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg                     <  1:1128465/143‑1 (MQ=25)
                                                                                                                                     aattccgagaaaaaaaaaaCCGCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt                      <  1:319457/135‑1 (MQ=25)
                                                                                                                                     aaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATaa                 <  2:897367/151‑1 (MQ=255)
                                                                                                                                        tCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcg              >  1:400973/1‑151 (MQ=255)
                                                                                                                                               aaaaaaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagc          <  2:832547/146‑1 (MQ=255)
                                                                                                                                               aaaaaaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcg              <  2:45973/142‑1 (MQ=255)
                                                                                                                                                gAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAt      >  1:939692/1‑151 (MQ=255)
                                                                                                                                                    aaaaCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAgg  >  2:929782/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
AGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGG  >  NC_000913/2001769‑2002065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: