Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,378,331 A→G I205T (ATT→ACT)  ampC ← penicillin‑binding protein; beta‑lactamase, intrinsically weak

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,378,3310AG98.5% 258.4 / ‑6.5 67I205T (ATT→ACT) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (38/27);  total (40/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATTGCTTTGCACCCAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCA  >  NC_000913/4378184‑4378477
                                                                                                                                                   |                                                                                                                                                  
aaaTTGCTTTGCACCCAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTcc                                                                                                                                                 <  1:660722/151‑1 (MQ=255)
aaaTTGCTTTGCACCCAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTcc                                                                                                                                                 <  2:272925/151‑1 (MQ=255)
aaaTTGCTTTGCACCCAGCGGGCCATATCTTCAAGGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTcc                                                                                                                                                 <  2:120368/151‑1 (MQ=255)
 aaTTGCTTTGCACCCAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTGCATTAGTcc                                                                                                                                                 >  2:1083334/1‑150 (MQ=255)
    tGCTTTGCACCCAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGt                                                                                                                                             >  2:1136763/1‑151 (MQ=255)
    tGCTTTGCACCCAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGt                                                                                                                                             >  2:378651/1‑151 (MQ=255)
           cACCCAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGttg                                                                                                                                      >  2:477270/1‑151 (MQ=255)
              ccAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGttga                                                                                                                                     <  1:302974/149‑1 (MQ=255)
                        atatCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCt                                                                                                                         >  1:750450/1‑151 (MQ=255)
                              tCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTgg                                                                                                                       <  2:329119/147‑1 (MQ=255)
                               cAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGac                                                                                                                  >  2:183525/1‑151 (MQ=255)
                                    gTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGa                                                                                                              <  2:1072813/150‑1 (MQ=255)
                                    gTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAg                                                                                                             <  1:205552/151‑1 (MQ=255)
                                    gTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGaa                                                                                                                     >  2:700269/1‑143 (MQ=255)
                                       gACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGttt                                                                                                          >  2:888514/1‑151 (MQ=255)
                                       gACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGttt                                                                                                          >  2:530008/1‑151 (MQ=255)
                                          ttCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGc                                                                                                        >  2:1077806/1‑150 (MQ=255)
                                            cacaCCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATc                                                                                                     >  1:808555/1‑151 (MQ=255)
                                             actccATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGGACTGCCTTTCCTTCGTGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACCCGAGTTTGCag                                                                                                      <  1:750374/146‑2 (MQ=255)
                                             acacCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCg                                                                                                    >  2:1237614/1‑151 (MQ=255)
                                                ccATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCt                                                                                                 <  1:189584/151‑1 (MQ=255)
                                                 cATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTg                                                                                                >  2:114713/1‑151 (MQ=255)
                                                  aTAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGc                                                                                               >  2:936553/1‑151 (MQ=255)
                                                  aTAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGc                                                                                               <  1:873604/151‑1 (MQ=255)
                                                           aGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTc                                                                                      >  2:891754/1‑151 (MQ=255)
                                                                  aaCGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAg                                                                               <  1:170288/151‑1 (MQ=255)
                                                                    cGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAATCTCAAACCAGAc                                                                             <  1:716341/151‑1 (MQ=255)
                                                                    cGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGAc                                                                             >  2:725769/1‑151 (MQ=255)
                                                                     gCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACg                                                                            >  2:782472/1‑151 (MQ=255)
                                                                         cAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCtt                                                                        >  1:125001/1‑151 (MQ=255)
                                                                         cAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGATGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGc                                                                          <  1:180569/149‑1 (MQ=255)
                                                                          aGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTc                                                                       >  2:509941/1‑151 (MQ=255)
                                                                          aGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTc                                                                       >  1:259419/1‑151 (MQ=255)
                                                                           ggCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTca                                                                      <  1:644570/151‑1 (MQ=255)
                                                                                aaCATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGcc                                                                 >  1:1142040/1‑151 (MQ=255)
                                                                                aaCATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGcc                                                                 >  2:46376/1‑151 (MQ=255)
                                                                                  cATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAg                                                               <  1:607195/151‑1 (MQ=255)
                                                                                   aTGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCTGTACATTATTCTACTTATGGTTGAGATTGAGTGGCTGGAAGACACTAGATTGCGTCGCCTGCTCAAAACTTATACCAGGCGGCTTTACAGCCAg                                                               >  1:717186/1‑150 (MQ=255)
                                                                                   aTGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGt                                                              >  1:904895/1‑151 (MQ=255)
                                                                                           ccTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGcc                                                         <  2:420779/148‑1 (MQ=255)
                                                                                           ccTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGaa                                                      <  2:510473/151‑1 (MQ=255)
                                                                                               aCCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACaa                                                   <  1:227451/150‑1 (MQ=255)
                                                                                               aCCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACaa                                                   >  2:861248/1‑150 (MQ=255)
                                                                                                ccTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACaaa                                                  <  2:29890/150‑1 (MQ=255)
                                                                                                ccTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAAc                                                 >  2:253245/1‑151 (MQ=255)
                                                                                                     gcgATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATa                                            <  1:403897/151‑1 (MQ=255)
                                                                                                      cgATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATAc                                           >  2:916095/1‑151 (MQ=255)
                                                                                                         tatCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTgg                                        >  1:375135/1‑151 (MQ=255)
                                                                                                          atCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTgg                                        <  1:285200/150‑1 (MQ=255)
                                                                                                             cccAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGtt                                    <  2:904960/151‑1 (MQ=255)
                                                                                                                aGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGc                                 >  2:578248/1‑151 (MQ=255)
                                                                                                                 ggCGTAATTCTTTTCTTCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGc                                 >  2:308514/1‑150 (MQ=255)
                                                                                                                        ttcttttcttCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGAc                         >  2:1070460/1‑151 (MQ=255)
                                                                                                                         tcttttcttCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGAc                         <  2:1152367/150‑1 (MQ=255)
                                                                                                                          cttttcttCTGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGt                       >  1:1082788/1‑151 (MQ=255)
                                                                                                                              tcttctGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTtgt                    <  2:80052/150‑1 (MQ=255)
                                                                                                                                 tctGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTgtgt                  <  2:750494/149‑1 (MQ=255)
                                                                                                                                  ctGCGGGCGGTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTcc               >  1:249702/1‑151 (MQ=255)
                                                                                                                                     cgggcggTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTgg            >  2:1077274/1‑151 (MQ=255)
                                                                                                                                     cgggcggTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTgg            >  2:959530/1‑151 (MQ=255)
                                                                                                                                     cgggcggTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAACACACGCGTTTGCATCGCCTTCTCAAAACTCAAATCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCt              >  1:37152/1‑149 (MQ=255)
                                                                                                                                      gggcggTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTg             >  2:942053/1‑149 (MQ=255)
                                                                                                                                         cggTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGcc        <  2:308436/151‑1 (MQ=255)
                                                                                                                                         cggTACATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGcc        >  1:278927/1‑151 (MQ=255)
                                                                                                                                          ggTACATTAGTCCACGTATGGTTGAGTTTTAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGccc       >  2:782520/1‑151 (MQ=255)
                                                                                                                                              cATTAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGc   >  2:73398/1‑151 (MQ=255)
                                                                                                                                                ttAGTCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCa  <  2:1231978/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAATTGCTTTGCACCCAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCA  >  NC_000913/4378184‑4378477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: