Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,966,573	T→G	D621A (GAT→GCT)	ptsP ←	PEP‑protein phosphotransferase enzyme I; GAF domain containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,966,573	0	T	G	100.0%	327.1 / NA	81	D621A (GAT→GCT)	ptsP	PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (39/42);  total (39/42)

CACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCAC  >  NC_000913/2966427‑2966714                                                                                                                                                   |                                                                                                                                              cACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGtt                                                                                                                                           <  1:472967/151‑1 (MQ=255)    cGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGt                                                                                                                                        >  2:207196/1‑151 (MQ=255)     gCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTg                                                                                                                                       >  2:1157290/1‑151 (MQ=255)        caAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGcc                                                                                                                                     >  1:953835/1‑150 (MQ=255)          aaCGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAAc                                                                                                                                  >  2:797943/1‑151 (MQ=255)             ggAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACaga                                                                                                                               <  1:861352/151‑1 (MQ=255)             ggAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACaga                                                                                                                               <  1:467859/151‑1 (MQ=255)              gagaTCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACagag                                                                                                                              <  2:545676/151‑1 (MQ=255)               agaTCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACag                                                                                                                                >  2:396561/1‑148 (MQ=255)               agaTCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAgaga                                                                                                                             <  2:444047/151‑1 (MQ=255)                gaTCGATTCCATGAATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACag                                                                                                                                >  2:1105979/1‑147 (MQ=255)                  tCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATg                                                                                                                           >  2:1223879/1‑150 (MQ=255)                  tCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATg                                                                                                                           >  1:276385/1‑150 (MQ=255)                    gATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGaa                                                                                                                         >  1:1240172/1‑150 (MQ=255)                     aTTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACGTTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAAt                                                                                                                       <  2:450643/151‑1 (MQ=255)                     aTTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAAt                                                                                                                       <  1:1154593/151‑1 (MQ=255)                       tCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGATGGTGCCAACCGAGATGAAATc                                                                                                                      <  1:1167862/150‑1 (MQ=255)                        ccATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGATGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCg                                                                                                                     <  1:393237/150‑1 (MQ=255)                         cATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGAc                                                                                                                   >  2:945242/1‑151 (MQ=255)                          aTGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGAcc                                                                                                                  >  1:827216/1‑151 (MQ=255)                           tGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGAccc                                                                                                                 >  2:797890/1‑151 (MQ=255)                            gTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCg                                                                                                                <  1:204275/151‑1 (MQ=255)                              aTTTCCGCTTCCCGAGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGc                                                                                                               >  2:165765/1‑150 (MQ=255)                                 tCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTg                                                                                                           >  1:108853/1‑151 (MQ=255)                                  ccGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTg                                                                                                           <  2:438434/150‑1 (MQ=255)                                    gCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGcc                                                                                                         >  2:795237/1‑150 (MQ=255)                                      ttCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGcc                                                                                                         >  2:275417/1‑148 (MQ=255)                                       tCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAg                                                                                                       <  1:119676/149‑1 (MQ=255)                                        cccGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGAt                                                                                                     <  2:942683/150‑1 (MQ=255)                                         ccGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGc                                                                                                   <  2:146062/151‑1 (MQ=255)                                             gCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgca                                                                                               <  1:832767/151‑1 (MQ=255)                                             gCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgca                                                                                               <  2:418421/151‑1 (MQ=255)                                              cGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgcag                                                                                              <  2:1034818/151‑1 (MQ=255)                                              cGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgcag                                                                                              <  1:343501/151‑1 (MQ=255)                                               gATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGgcagc                                                                                             <  2:696049/151‑1 (MQ=255)                                                      gCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAAt                                                                                       >  2:315985/1‑150 (MQ=255)                                                        cAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATAcc                                                                                    >  1:730552/1‑151 (MQ=255)                                                             cTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTg                                                                                >  1:491663/1‑150 (MQ=255)                                                                 tAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGc                                                                           >  1:309090/1‑151 (MQ=255)                                                                     aTTGCAGGATGAAGACTGGCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACtt                                                                       >  1:468505/1‑151 (MQ=255)                                                                          aGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGc                                                                  <  2:655716/151‑1 (MQ=255)                                                                             aTGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCat                                                                >  2:1213709/1‑150 (MQ=255)                                                                              tGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCatg                                                               >  1:591437/1‑150 (MQ=255)                                                                                aaGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCatgatg                                                            >  1:564664/1‑151 (MQ=255)                                                                                  gACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGcc                                                          <  2:582985/151‑1 (MQ=255)                                                                                   aCTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCg                                                         <  2:566027/151‑1 (MQ=255)                                                                                      gTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGAt                                                       >  2:237464/1‑150 (MQ=255)                                                                                       tCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATAc                                                     >  1:522994/1‑151 (MQ=255)                                                                                        cATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACg                                                    >  2:858512/1‑151 (MQ=255)                                                                                         aTAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGt                                                   <  2:513755/151‑1 (MQ=255)                                                                                           aaaTGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTgg                                                 <  2:1122981/151‑1 (MQ=255)                                                                                           aaaTGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTgg                                                 >  2:826563/1‑151 (MQ=255)                                                                                           aaaTGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTg                                                  <  2:529789/150‑1 (MQ=255)                                                                                            aaTGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGt                                                <  2:1093543/151‑1 (MQ=255)                                                                                              tGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGttt                                              >  2:607619/1‑151 (MQ=255)                                                                                              tGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGttt                                              >  1:882286/1‑151 (MQ=255)                                                                                               gTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGttt                                              <  2:79650/150‑1 (MQ=255)                                                                                                  cGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTggg                                           <  2:171540/150‑1 (MQ=255)                                                                                                    ccACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAt                                        <  2:1220032/151‑1 (MQ=255)                                                                                                     cACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAtt                                       >  1:557215/1‑151 (MQ=255)                                                                                                      aCCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAAttt                                      >  2:904817/1‑151 (MQ=255)                                                                                                         cGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGt                                   >  1:547957/1‑151 (MQ=255)                                                                                                          gggTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTa                                  <  1:95676/151‑1 (MQ=255)                                                                                                          gggTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTa                                  <  2:172764/151‑1 (MQ=255)                                                                                                          gggTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTa                                  <  2:154937/151‑1 (MQ=255)                                                                                                           ggTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTaa                                 <  1:1080231/151‑1 (MQ=255)                                                                                                              aTTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCg                              <  2:970033/151‑1 (MQ=255)                                                                                                                 gttgCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatc                           <  2:930483/151‑1 (MQ=255)                                                                                                                 gttgCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGat                            <  1:657575/150‑1 (MQ=255)                                                                                                                   tgCGATCAACGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatcat                         >  2:404542/1‑151 (MQ=255)                                                                                                                   tgCGATCAACGACCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGatcat                         >  2:1069706/1‑151 (MQ=255)                                                                                                                            cGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCATCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGAc                <  1:165761/151‑1 (MQ=255)                                                                                                                            cGGCCAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCAATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGAc                <  2:106651/151‑1 (MQ=255)                                                                                                                               ccAGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTc             >  2:930364/1‑151 (MQ=255)                                                                                                                                 aGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCAc           >  2:1016282/1‑151 (MQ=255)                                                                                                                                 aGAATGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCAc           <  1:1157200/151‑1 (MQ=255)                                                                                                                                   aaTGTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGt         >  2:1243956/1‑151 (MQ=255)                                                                                                                                      gTATTGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTcc       <  1:1210273/150‑1 (MQ=255)                                                                                                                                         ttGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGc    >  2:245402/1‑150 (MQ=255)                                                                                                                                          tGAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCa   <  1:1223784/150‑1 (MQ=255)                                                                                                                                           gAGTCAGAGCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCAc  <  2:1243780/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                              CACCGCACAAACGGAGATCGATTCCATGTATTTCCGCTTCCCGGGCGATCATCGCCAGAGCTCGTAACATTGCAGGATGAAGACTGTCATAAATGTTCGCCACCCGGGTATTGTTGCGATCAACGGCCAGAATGTATTGAGTCAGATCGTTGGTGCCAACAGAGATGAAATCGACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCAC  >  NC_000913/2966427‑2966714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑