Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,310,266	C→A	E301D (GAG→GAT)	pnp ←	polynucleotide phosphorylase/polyadenylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,310,266	0	C	A	100.0%	326.3 / NA	83	E301D (GAG→GAT)	pnp	polynucleotide phosphorylase/polyadenylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/51);  total (32/51)

GCGCCTGCGTTTCACCACGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTCTCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTGATGCGGTAAGCATCGCTCAGGCG  >  NC_000913/3310118‑3310412                                                                                                                                                     |                                                                                                                                                   gcgcCTGCGTTTCACCACGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTtatc                                                                                                                                                  <  2:802455/151‑1 (MQ=255)  cgcCTGCGTTTCACCACGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCg                                                                                                                                                 <  2:978543/151‑1 (MQ=255)   gcCTGCGTTTCACCACGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCg                                                                                                                                                 >  1:878801/1‑150 (MQ=255)    ccTGCGTTTCACCACGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCg                                                                                                                                                 <  1:879377/149‑1 (MQ=255)     cTGCGTTTCACCACGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATc                                                                                                                                              >  1:341050/1‑151 (MQ=255)     cTGCGTTTCACCACGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATc                                                                                                                                              >  1:82664/1‑151 (MQ=255)       gCGTTTCACCACGGGTGAACAGCGCAGAACCGTGAGTCCGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTatcgatcg                                                                                                                                             <  2:902291/150‑1 (MQ=255)        cGTTTCACCACGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATcgcg                                                                                                                                           >  2:820092/1‑151 (MQ=255)          tttCACCACGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTg                                                                                                                                         >  2:627654/1‑151 (MQ=255)             caccacGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAg                                                                                                                                      <  2:1016640/151‑1 (MQ=255)               ccccGGGTGAACAGCGCAGACCCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGaa                                                                                                                                    <  1:761210/148‑1 (MQ=255)               ccacGGGTGAACGGCGCAGAACCGGGAGGCCGCGGCAGCCCGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCCCGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGa                                                                                                                                     <  1:690287/150‑1 (MQ=255)                   gggTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTc                                                                                                                                >  2:81616/1‑151 (MQ=255)                     gTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCAc                                                                                                                              <  2:199904/151‑1 (MQ=255)                        aaCAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCa                                                                                                                           <  2:1061638/151‑1 (MQ=255)                         aCAGCGAAGAACCGTGAGTACGCGGCACCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAg                                                                                                                          <  2:86293/151‑1 (MQ=255)                          cAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGt                                                                                                                         >  1:366675/1‑151 (MQ=255)                                aGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGttt                                                                                                                   >  1:40308/1‑151 (MQ=255)                                 gAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGtttt                                                                                                                  >  2:959462/1‑151 (MQ=255)                                  aaCCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGtttt                                                                                                                  <  2:390814/150‑1 (MQ=255)                                  aaCCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGtt                                                                                                                    <  1:230132/148‑1 (MQ=255)                                   aCCGTGAGTACGAGGCAGCACGCCAGTACGCACATCCAGCCCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCg                                                                                                                <  2:365611/151‑1 (MQ=255)                                    ccGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGt                                                                                                               >  2:361701/1‑151 (MQ=255)                                     cGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCg                                                                                                                <  1:1155245/149‑1 (MQ=255)                                     cGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTc                                                                                                              <  2:1009139/151‑1 (MQ=255)                                      gTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGCCCACGGATCATATCTTTTTCCCGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTc                                                                                                              <  1:1027842/150‑1 (MQ=255)                                      gTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTcc                                                                                                             >  1:131868/1‑151 (MQ=255)                                      gTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTcc                                                                                                             <  2:551332/151‑1 (MQ=255)                                            aCGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGt                                                                                                        <  1:188237/150‑1 (MQ=255)                                                ggCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTc                                                                                                     <  1:1077477/149‑1 (MQ=255)                                                    gcaCGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACACAGTTCGTTTTCGTCCAGGGTTTCGTCTTc                                                                                               >  2:718309/1‑151 (MQ=255)                                                      aCGCCAGTACGCACATCCAGACCACGTATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCa                                                                                              <  1:572412/150‑1 (MQ=255)                                                       cGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCagc                                                                                            <  1:1078070/151‑1 (MQ=255)                                                        gCCAGTACGCACATCCAGCCCACGGATCATATTTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCagc                                                                                            <  1:903931/150‑1 (MQ=255)                                                         ccAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCagcaa                                                                                          <  2:445668/151‑1 (MQ=255)                                                          cAGTACGCACATCCAGACCACGGTTCATATCTTTTTCACGACCGTCGATACCCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCagca                                                                                           <  1:18883/149‑1 (MQ=255)                                                           aGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATCCGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCagcaagc                                                                                        <  1:51130/151‑1 (MQ=255)                                                           aGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCagcaag                                                                                         <  1:47651/150‑1 (MQ=255)                                                            gTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCagcaagca                                                                                       <  2:788802/151‑1 (MQ=255)                                                            gTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCagcaagca                                                                                       <  2:1128326/151‑1 (MQ=255)                                                             tACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCagcaagc                                                                                        >  2:43855/1‑149 (MQ=255)                                                              aCGCCCATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAagcagc                                                                                     <  1:81970/151‑1 (MQ=255)                                                              aCGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAagcagc                                                                                     >  2:524189/1‑151 (MQ=255)                                                              aCGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAagcag                                                                                      <  2:426844/150‑1 (MQ=255)                                                               cGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCagcaagca                                                                                       <  2:892185/148‑1 (MQ=255)                                                                        aGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATgg                                                                           >  1:56739/1‑151 (MQ=255)                                                                        aGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATgg                                                                           <  1:1038657/151‑1 (MQ=255)                                                                           ccACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGttt                                                                        >  2:91666/1‑151 (MQ=255)                                                                            cACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGttt                                                                        >  1:568251/1‑150 (MQ=255)                                                                            cACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTc                                                                       <  2:999338/151‑1 (MQ=255)                                                                            cACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTc                                                                       >  1:552665/1‑151 (MQ=255)                                                                                 aTCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGAtt                                                                  >  1:414181/1‑151 (MQ=255)                                                                                  tCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGAttt                                                                 >  2:620665/1‑151 (MQ=255)                                                                                   cATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTtg                                                                >  2:191924/1‑151 (MQ=255)                                                                                       tCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTtgatg                                                             >  1:1008093/1‑150 (MQ=255)                                                                                       tCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTtgatg                                                             >  2:347075/1‑150 (MQ=255)                                                                                         tttttCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGAc                                                           >  1:393030/1‑150 (MQ=255)                                                                                           tttCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACAt                                                         <  2:936543/150‑1 (MQ=255)                                                                                                cGACCGTCGATTCGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCaa                                                      <  1:84806/148‑1 (MQ=255)                                                                                                cGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCt                                                   >  1:1235297/1‑151 (MQ=255)                                                                                                  aCCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCt                                                   <  1:439015/149‑1 (MQ=255)                                                                                                   ccGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTgcg                                                <  1:255313/151‑1 (MQ=255)                                                                                                    cGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTgcgc                                               >  1:911148/1‑151 (MQ=255)                                                                                                     gTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTgcgc                                               >  2:817639/1‑150 (MQ=255)                                                                                                      tCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCAt                                             >  2:781406/1‑151 (MQ=255)                                                                                                       cGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCAt                                             >  1:558679/1‑150 (MQ=255)                                                                                                       cGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCAt                                             <  2:1184435/150‑1 (MQ=255)                                                                                                       cGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATa                                            <  1:428417/151‑1 (MQ=255)                                                                                                         aTACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAAc                                          <  2:1142647/151‑1 (MQ=255)                                                                                                         aTACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAAc                                          <  2:847278/151‑1 (MQ=255)                                                                                                               ggTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCtt                                    <  2:195502/151‑1 (MQ=255)                                                                                                                   cGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCttgttt                                <  1:689273/151‑1 (MQ=255)                                                                                                                   cGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCttgt                                  <  1:529312/149‑1 (MQ=255)                                                                                                                   cGCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCttgt                                  <  2:930217/149‑1 (MQ=255)                                                                                                                    gCCTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCttgtttg                               <  2:354169/151‑1 (MQ=255)                                                                                                                     ccTGCCAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCttgttt                                <  1:358712/149‑1 (MQ=255)                                                                                                                          cAGTACGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTg                         <  1:473848/151‑1 (MQ=255)                                                                                                                              aCGCGGCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTGATGc                     <  2:1032156/151‑1 (MQ=255)                                                                                                                                   gCTACGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTGATGCGGTaa                <  1:671269/151‑1 (MQ=255)                                                                                                                                      aCGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTGATGCGGTAAGc              <  1:485007/150‑1 (MQ=255)                                                                                                                                       cGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTGATGCGGTAAGCAt            >  1:79536/1‑151 (MQ=255)                                                                                                                                       cGAACAACGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTGATGCGGTAAGCAt            <  2:1008167/151‑1 (MQ=255)                                                                                                                                           caacGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTGATGCGGTAAGCATCGCt        <  1:931478/151‑1 (MQ=255)                                                                                                                                              cGTTTTTATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTGATGCGGTAAGCATCGCTc       >  1:1099624/1‑149 (MQ=255)                                                                                                                                                  tttATCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTGATGCGGTAAGCATCGCTCAGGCg  >  2:1082161/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GCGCCTGCGTTTCACCACGGGTGAACAGCGCAGAACCGTGAGTACGCGGCAGCACGCCAGTACGCACATCCAGACCACGGATCATATCTTTTTCACGACCGTCGATACGCGGTTCGCCTGCCAGTACGCGGCTACGAACAACGTTTTTCTCGATCGCGTGCAGAATTTCACCCAGTTCGTTTTCGTCCAGGGTTTCGTCTTCAGCAAGCAGCGTCGCGATGGTTTCAGATTTGATGACATCAACCTGCGCATAACGCTCTTGTTTGTCGGTGATGCGGTAAGCATCGCTCAGGCG  >  NC_000913/3310118‑3310412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑