Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 73,413 | 0 | A | C | 38.9% | 82.3 / 16.0 | 55 | V370G (GTG→GGG) | thiP | thiamine/thiamine pyrophosphate ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base A (25/8); new base C (0/21); total (25/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.12e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCAC > NC_000913/73271‑73561 | gATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGAccc > 1:152714/1‑151 (MQ=255) gATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGAccc < 2:1094402/151‑1 (MQ=255) gCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGCGTTGTTGAGCAGGAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGcc > 1:272454/1‑150 (MQ=255) ccATTAACGCATTGGTGAAAATCACAATCCCGTCGGCAGATGGTGGCAGCCCGATAGTGTTTTGGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCCCCAGCACCTGACCCGCCa < 2:843154/150‑1 (MQ=255) gCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCt > 1:1137063/1‑151 (MQ=255) ggTGAAAATCAAAATGCCGTCAGCAGTTTGGGGCAGGCCGATAGTTTTTTTGAGAAGAAAAAAGAAGCCGTTAGCCAGCACAACCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCcg < 2:48332/150‑1 (MQ=255) ggTAAAAAACAAAGTGCCGCAAGCAGTTTGGGCAAGGCCGATCGGGTAGTTGAGAAGAAAAAAGAAGCGGGTAGCCACCACAACCCCGGGC‑ATGGCGAGG‑ATCAACAT‑GCCCCTCATCCCCAGCACCTGACCCGCCAGCATTTTCTg < 2:67649/147‑1 (MQ=255) ataaaaACACAGTGCCGAAAGGGGAGTGTGGCAGGCCAATAGTGTTTTGGAGAAGTAACAAGACGCGGGTACCCTTCCCATCCCCCGGC‑TGGTGGAGG‑ACCAAACG‑GCCGCCTCTCCCCACCACCGCAGCCGCCAGCATTTTCTGCcgcg < 2:1110955/148‑1 (MQ=255) aaaTCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGccc > 2:6362/1‑150 (MQ=255) aTCACAATGCCGTCAGCAGATTGTGGCAGGCGGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCa < 1:168428/151‑1 (MQ=255) tGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTcgc > 2:1003589/1‑150 (MQ=255) ggctgTCACCATATTGTGGCGGGCCAATTGTTTTTTTGCGCAGTAAAAAAGGACCGGTGCCCCTCACCCCCCCGGTC‑CTGGCGGGT‑AACACTGT‑GCCACCCATCCCCACCATCCTTCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTcg < 2:1144546/145‑1 (MQ=255) gCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTcgcg > 2:28764/1‑150 (MQ=255) ccGTCAGCAGATTGGGGCAGGCCGATAGTGTTGTTGAGCAGAAAAAAGAAGCCGGTAGCCAGCACAACCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGAc < 1:896626/151‑1 (MQ=255) cgtgggaagATTGTGGCGGGCGGTAAGTGTTTTTGACCGTAAAAAAGAAGCCGGTAGCCACCCCAATCCCCGGC‑TGGGCGAGG‑TTAAACTT‑GCCGCTCTTCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCa < 1:693579/134‑1 (MQ=255) gTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTg > 1:510821/1‑151 (MQ=255) tCAGCAGATTGTGGCAGGCCGATAGTGCTGTTGAGCAGTAAAAAGAAGCCGGGAGCCAGCACAATCCCCGGC‑GTGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGc > 2:235639/1‑151 (MQ=255) gATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCAGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCAt > 2:494429/1‑150 (MQ=255) ttGTGGCGGGCCGATAGTTTTGTTGAGCATAAAAAAGAAGCCGGTAGCCAGCACAACCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCAt < 1:432071/148‑1 (MQ=255) ttGGGGCAGGCGGTTAGTTTTGTGGAGCATAAAAAAGAGCCCGGTACCCACCACACCCCCCGGC‑ATGGGGAGG‑ATCACCAT‑GCCGCTCATCTCCAGCCCCTAACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCAt < 2:1016551/148‑1 (MQ=255) ggcgggcgGATAGTGTTGTGGAGCAGAAAAAAGAGGCCGGTAGCCACCACAACCCCGGGC‑AGGGCGAGG‑ATCAACAT‑GCCGCTCATCCCCAGCCCCGGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTg < 2:628521/138‑1 (MQ=255) ggCAGGCCGATAGTGTTTTTGAGCAGAAAAAAGAAGCCGGTACCCAGCACAATCCCCGGC‑TTGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagca < 1:410629/148‑1 (MQ=255) ggCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑AGGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATagcagc < 1:581268/150‑1 (MQ=255) gATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTc > 2:1097362/1‑151 (MQ=255) gtgtTGTTGAGCAAAAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCa < 1:1083606/148‑1 (MQ=255) gttgttGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACt > 2:819128/1‑151 (MQ=255) gttgtgGAGCAGAAAAAAGAAGCCGGTGGCCAGCACAACCCCCGGC‑TTGGCGAGG‑ATAAACAG‑GCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAg < 2:261554/147‑1 (MQ=255) ttgGGCAGAAAAAAGAAGCCGGTACCCACCACAACCCCCGGC‑ATGGGGAGG‑ATCAACAG‑GCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTAc < 1:850557/149‑1 (MQ=255) gAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGcac > 2:796957/1‑151 (MQ=255) gAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑AGGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCCCCGGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc < 1:220380/149‑1 (MQ=255) aaaaataaaaaaaaaGCCGTCCGCCAGCAC‑ACCCCCGGA‑CTTGCCAGGAATAAACTG‑CCCGCGCATTTCCAGCTCCCGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGca < 2:176375/143‑1 (MQ=255) gCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGcaca > 2:16431/1‑150 (MQ=255) cgggaaaaaaGAAGCGGCTGGCAAGCAC‑ATCCCCGGCAAGGGCGAGG‑A‑CAAAATCGCCATAATTCCACAGCCCCAGGCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACt < 2:594251/138‑1 (MQ=255) aaaaaGAACCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCCCCACCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATacac < 1:901197/151‑1 (MQ=255) aaaaGAACCCGGTAGCCAGCACAACCCCCGGC‑ATGGCGAGG‑ATCAACAG‑GCCGCTCACCCCCAGCACCGACCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACAcc < 1:364546/151‑1 (MQ=255) agCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTgccgc > 2:1051114/1‑151 (MQ=255) gCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTgccgcc > 1:1098422/1‑151 (MQ=255) ccGGTAGCCGCCACAATCCCCGTG‑ATGGCGGGG‑ACAACCAT‑GCCGACCATCACCAGCCCCGGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCa < 2:441474/151‑1 (MQ=255) ccGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCa < 1:1075900/151‑1 (MQ=255) tAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAgcgc > 1:468534/1‑151 (MQ=255) aGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAgcgc > 2:1096079/1‑150 (MQ=255) ccaccaCAACCCCGGGC‑AGGGCGAGC‑ACCAACAT‑CCCCCCCTCCCCCGCCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAgcgc < 2:416524/148‑1 (MQ=255) aGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTCCGCACAATACACCTGCCGCCAGCGCAATAc > 1:690113/1‑151 (MQ=255) accccccGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGc < 1:1152297/145‑1 (MQ=255) aaTCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAAc > 2:1062964/1‑151 (MQ=255) cccgGGC‑AGGGCGAGA‑ATCAACAT‑GCCGCTCACCTCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAg < 1:743023/151‑1 (MQ=255) tGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCcac > 2:615945/1‑149 (MQ=255) ggggAGG‑ATCAACAG‑GCCGCTCATCCCCAGCCCCGGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCcaca < 2:875541/146‑1 (MQ=255) ggCGAGG‑ACCAACAG‑GCCGCTCACCCCCAGCCCCGGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCcac < 2:98919/148‑1 (MQ=255) cGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAgcgc > 2:224410/1‑151 (MQ=255) gg‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTg > 2:210607/1‑151 (MQ=255) gg‑ATCAACAT‑GCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCt < 1:471050/150‑1 (MQ=255) cGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCgg > 1:161948/1‑151 (MQ=255) gCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGt > 1:1046588/1‑151 (MQ=255) gCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCAc > 2:370543/1‑151 (MQ=255) | GATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGC‑ATGGCGAGG‑ATCAACAT‑GCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCAC > NC_000913/73271‑73561 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |