Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,268	0	T	A	48.4%	54.7 / 46.4	64	L24F (TTA→TTT)	emrK	multidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (25/8);  new base A (0/31);  total (25/39)
Fisher's exact test for biased strand distribution p-value = 3.65e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGC  >  NC_000913/2483118‑2483417                                                                                                                                                         |                                                                                                                                                       gTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑aataaat                                                                                                                                                        >  2:703617/1‑151 (MQ=255)     gTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGGGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AAtaaataaa                                                                                                                                                     >  1:415496/1‑150 (MQ=255)      tATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑c                                                                                                                                                  >  1:879725/1‑151 (MQ=255)           ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑Ct                                                                                                                                                 <  2:951482/147‑1 (MQ=255)                    aCGACAGTGACCCTACTTGAGATTTGGGAAAAATTTGGATCTGCATTCCCCGTGACATAGGCGTCTTCTGTACTAATCTTGTTTTATAATTCCATAGCCC‑AATAGGAAGAGCCCCCTAAAAACAA‑AAAAAAAAAAA‑CTACCGCCAATAAAg                                                                                                                                    <  2:765915/151‑1 (MQ=255)                      gACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGa                                                                                                                                   >  1:237172/1‑150 (MQ=255)                          gTGACCCTCCCTGAGACTTGTGAAAAAATTGGATCTGCATCCCCCGTGACATAGGCTTCATCTGTACTAATCATTTTTTCAAATCCCATTGCCA‑AAAAGAAAAAGGCCCCTAAAAACAC‑AAAAAAAAAAC‑CTACCGCCAATAAAGAAAAGt                                                                                                                              <  2:787586/151‑1 (MQ=255)                                 tACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTttct                                                                                                                       >  2:706931/1‑151 (MQ=255)                                    cagaattttGTGCAGAAATTGGATCTGCTTCCCCGGAAACAGCGGCATTTCTTCTACAAATCTTGTCTTCTAATTCCATCAACC‑AATGGGAATAGTCACTAAAAACCAA‑AATAAATAAAA‑GTACCGCCAATAAAGAAAAGTATTttct                                                                                                                       <  2:649995/141‑1 (MQ=255)                                     tGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTg                                                                                                                   >  1:631774/1‑151 (MQ=255)                                       agttttGTGAAAAAATTGGATCTGCATTCCCCGTAACAAAGGCGTAATCTGTACTAATAATTTCTTCTAATCCCATTCCCA‑AAAAGACATAGCCACCTAAAACCAA‑AAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTg                                                                                                                   <  2:8102/145‑1 (MQ=255)                                       agctttGTGAAAAAATGGGCTTTGCTTTCCCCGTGAACTAGCGTTCATGTATACAAATCAGGCCTTATAATCCCATTCACC‑AAAAGGCTTAGCCCCTTAAAAGCAC‑AATAAAAAAAA‑CTGCCGCCAATAAAGAAAAGTATTTtcttct                                                                                                                    <  1:65788/144‑1 (MQ=255)                                           ttgtaaaaaaaTTGGCTCTCCATTCCCCGTGAAATAGGCGTCATCTTTATTAATCATGTCTATTAATCCCATTGACA‑AAAAGGCAGAGGC‑CCAAAAAACCCAAAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTa                                                                                                                <  1:42151/140‑1 (MQ=255)                                            tgtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt                                                                                                            >  2:869464/1‑151 (MQ=255)                                             gtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑GATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATg                                                                                                           >  2:1051914/1‑151 (MQ=255)                                             gtgAAGAAATTGGATCCGCTTCCCCCGTGCCAAAGGCGTCTTTTGAACAAATCATGTTTTTTAATCCCATTGACC‑AATAGGCAAAGGCCCATAAAACCAA‑AAAAAATAAAT‑CTGCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa                                                                                                             <  1:633533/149‑1 (MQ=255)                                                   aaaTTGGTTCTCCATCCCCCGTAACATGGGTGTCATTTTTACTAATCATTTCTTATAATTCCATTACCA‑AAAAGAAATAGCCACTTAAAAACGA‑AAAAAAAAAAA‑TTGCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttttt                                                                                                      <  2:429177/150‑1 (MQ=255)                                                    aaTTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttttt                                                                                                     >  1:161740/1‑150 (MQ=255)                                                    aaTTGGATCTGCATTCCCCGTAACATAGGCGTCTTCTGTCCTAATCTTGTCTTCAAATCCAATTGACA‑AATGGGCATGGGCACCTAAAAACGA‑AATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa                                                                                                    <  1:51087/151‑1 (MQ=255)                                                        gtaTCTTCTTTCCCCGTGAAATAGGCGTCATCTGTACAAATCATTTTTTCAAATTCCATTGCCA‑AAAAGGAATAGGCCCTTAAAAACGA‑AATAAAAAAAT‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAtt                                                                                                  <  2:551525/147‑1 (MQ=255)                                                        ggATCTGCTTTCCCCGTGACATAGGCGTCATCTTTCCAAATCATGTTTTTTAATTCCATTGACC‑AAAAGGCAAAGGCCCCAAAAAACGC‑AATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTg                                                                                                <  1:406904/151‑1 (MQ=255)                                                        ggATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTg                                                                                                >  2:800669/1‑151 (MQ=255)                                                        ggATCTGCATTCCCCGTGAAATGGGCGTCATCTGTACTAATCATGTTTTCTAATTCCATTGACA‑AAAAGGCATAGGCACCTAAAAACGC‑AAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAttt                                                                                                 <  1:1142180/150‑1 (MQ=255)                                                        gaaTCTCCATTCCCGGTAACATAGGGTTCATTTGTACTAATCATGTTTTCAAATTCCATTGACA‑AAAAGGCATGGCCCCCTAAAAACAA‑AATAAATAAAC‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAttt                                                                                                 <  1:1043513/148‑1 (MQ=255)                                                                atcccccGTAACAAAGGCGTCCTCTGTACTAATCATGTTTTCAAATCCCATTGACC‑AATAGGAATAGGCACTTAAAACCGC‑AAAAAAAAAAT‑TTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAtt                                                                                            <  2:37014/144‑1 (MQ=255)                                                                aTTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAAt                                                                                         >  1:196226/1‑150 (MQ=255)                                                                 ttCCCCGTGACATAGGCGTCATCTGTACTAATCATTTCTTCCAATTCCATTAACA‑AATAGGCATAGGCACCTAAAAACGC‑AAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa                                                                                           <  1:427277/147‑1 (MQ=255)                                                                 tcccccGTACCATGGGCGCCATCTGTCCTAATAATTTTTTCTAATTCCATTAACA‑AATAGGCATAGGCCCCTAAAACCGA‑AAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGa                                                                                               <  1:115078/141‑1 (MQ=255)                                                                  tCCCCGTGACATAGGCGTCATCTGAACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt                                                                                       >  2:446362/1‑150 (MQ=255)                                                                   ccccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt                                                                                     >  2:863715/1‑151 (MQ=255)                                                                   ccccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTAAAAACGA‑AATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTg                                                                                      <  1:186320/150‑1 (MQ=255)                                                                   ccccGTAACATAGGCTTCATTTGTACTAAACATTTCTTCT‑ATTCCAATTACCAAAAAGAAATAGCCACCTAAAAACGA‑AAAAAAAAAAT‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTaa                                                                                          <  2:1053606/146‑1 (MQ=255)                                                                   ccccGCGACATAGGCATCATCTTTACTAACCATTTCTTCAAATCCCTTACACA‑AATAGGCAT‑GGCCCCCAAAAACCC‑AAAAAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt                                                                                     <  1:329467/151‑1 (MQ=255)                                                                       gTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTcc                                                                                  >  2:81329/1‑150 (MQ=255)                                                                         gACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt                                                                               >  1:1191391/1‑151 (MQ=255)                                                                          aCATGGGCTTCATCTGTACTAATCTTTTTTTCTAATTCCATTGACA‑AATAGGCATAGGCACCTGAAAACGC‑AATAAAAAAAA‑TTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACta                                                                              <  1:1149061/151‑1 (MQ=255)                                                                          aCATAGGCGTCATCTGTCTTAATCATTTTTTCTAATCCCATTCACA‑AATAGGCATAGGCACCTGAAAACAC‑AATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTcc                                                                                  <  1:1097552/147‑1 (MQ=255)                                                                          aCATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCAATTAACA‑AATAGGCATAGCCCCCTAAAAACGA‑AATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACta                                                                              <  1:555288/151‑1 (MQ=255)                                                                               ggCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtc                                                                         >  2:866225/1‑151 (MQ=255)                                                                                 cGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctc                                                                       >  1:893447/1‑151 (MQ=255)                                                                                  gTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctct                                                                      >  2:1160826/1‑151 (MQ=255)                                                                                        tGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc                                                                >  1:1035101/1‑151 (MQ=255)                                                                                             tAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                            >  1:1037869/1‑150 (MQ=255)                                                                                              aataatGTCTTCTAATTCCATTGCCC‑AATAGGCATAGGCACGAGAAATCGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAg                                                          <  1:750034/151‑1 (MQ=255)                                                                                                 cATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg                                                       >  2:1183739/1‑151 (MQ=255)                                                                                                  attttttctAATTCCATTGCCA‑AATAGGCATAGGCCCCTAAAAACGA‑AAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGAt                                                        <  2:754006/144‑1 (MQ=255)                                                                                                  aTGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg                                                       >  1:1020233/1‑150 (MQ=255)                                                                                                   tGTTTTCTAATTCCATTGACA‑AAAAGGCAAAGCCACCTAAAAACGA‑AAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa                                                      <  1:274969/150‑1 (MQ=255)                                                                                                   tGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa                                                      >  2:377896/1‑150 (MQ=255)                                                                                                   tGTCTTCTAATCCCATTGACC‑AAAAGGCATAGGCACCTGAAACCGC‑AATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa                                                      <  1:1164313/150‑1 (MQ=255)                                                                                                   tGTCTTAAAATTCCATTGACA‑AAAAGGAATAGCCACTGAAAAACGA‑AAAAAAAAAAC‑TTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa                                                      <  2:448631/150‑1 (MQ=255)                                                                                                        tcaaaTCCCATCGACC‑AATAGGAATAGGCACCAGAAACCCA‑AATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAt                                                   <  2:415483/145‑1 (MQ=255)                                                                                                           aaTTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtg                                              >  2:848336/1‑150 (MQ=255)                                                                                                           aaTTCCATTGACC‑AAAAGGAATAGGCACCTAAAAACGA‑AAAAAAAAAAT‑TTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAt                                                   <  2:982376/145‑1 (MQ=255)                                                                                                           aaTCCCATTGACC‑AATAGGCATAGGCACCTAAAAACGA‑AAAAAAAAAAA‑CAACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtga                                             <  1:703642/151‑1 (MQ=255)                                                                                                               ccATTGACA‑AATAGGCATAGGCACCTGAAAACGC‑AATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaatt                                          <  1:3985/150‑1 (MQ=255)                                                                                                                cATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattg                                         >  1:597143/1‑150 (MQ=255)                                                                                                                     aCC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatga                                    >  1:394394/1‑150 (MQ=255)                                                                                                                      cc‑AAAAGGCATAGGCCCCTGAAACCGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattgaat                                      <  1:740474/147‑1 (MQ=255)                                                                                                                      acaaaaagaaaaaggcaCTTGAAAACTA‑AAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattgaat                                      <  2:196222/136‑1 (MQ=255)                                                                                                                         aaTAGGCATAGGCACCTGAGAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa                                  >  2:348308/1‑149 (MQ=255)                                                                                                                                          aaaaaCGA‑AAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc                  <  2:535771/147‑1 (MQ=255)                                                                                                                                          aaaaaCAA‑AAAAAATAAAA‑TTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCata               <  2:326244/150‑1 (MQ=255)                                                                                                                                           aaaaCGC‑AAAAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat                <  1:864691/149‑1 (MQ=255)                                                                                                                                           aaaaCAC‑AAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat                <  2:830113/149‑1 (MQ=255)                                                                                                                                           aaaaCAA‑AAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTGGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCat                <  1:965654/149‑1 (MQ=255)                                                                                                                                                 aaaaaaaataaaA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAac         <  1:986790/146‑1 (MQ=255)                                                                                                                                                   aataaataAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac       >  1:576548/1‑151 (MQ=255)                                                                                                                                                   aaaaaaaaaaC‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAac         <  1:56448/146‑1 (MQ=255)                                                                                                                                                     taaataaaA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac       >  1:1000878/1‑149 (MQ=255)                                                                                                                                                        ataaaA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGc  >  2:930959/1‑151 (MQ=255)                                                                                                                                                         taaaA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGc  >  1:429237/1‑150 (MQ=255)                                                                                                                                                         |                                                                                                                                                       GTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACC‑AATAGGCATAGGCACCTGAAAACGC‑AATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTGC  >  NC_000913/2483118‑2483417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑