Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 266,349 | 0 | C | A | 39.0% | 126.7 / 20.4 | 59 | V69F (GTT→TTT) | yafY | lipoprotein, inner membrane; degP regulator; CP4‑6 prophage |
Reads supporting (aligned to +/- strand): ref base C (7/29); new base A (23/0); total (30/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.74e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATG > NC_000913/266205‑266495 | ccGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCaa < 1:346119/151‑1 (MQ=255) ccGTCACCTCCTGGTATTCCAGTTTGATGTGATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCaa < 1:649791/151‑1 (MQ=255) cGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAg < 2:605924/151‑1 (MQ=255) gTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAA‑GT‑AAAAACTGCaa > 1:703768/1‑149 (MQ=255) cctcctGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCCTCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTggg < 1:95034/151‑1 (MQ=255) cctcctGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GT‑AAAAACCGCAAGGTggg > 1:1161246/1‑151 (MQ=255) ctcctGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GG‑AAAACACGCAAgggg > 2:205451/1‑146 (MQ=255) ctcctGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GT‑AAAAACCGCAAgggggg > 1:322884/1‑150 (MQ=255) ctcctGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑AT‑AAAAACACCAAgggggg > 2:117139/1‑150 (MQ=255) ctGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GG‑AAAAACTGCCAAGGGGGAcc > 1:1080199/1‑150 (MQ=255) ttCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGGA‑AAAACCTCCAAGG‑GGGGCCGGGTaa > 1:937657/1‑150 (MQ=255) ccAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAATGGCCGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAa‑gta < 2:302469/151‑1 (MQ=255) tttGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAAACTGCCAGGGGGGGCCGGaaaaag > 2:559177/1‑144 (MQ=255) aTGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCGGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCt < 2:1094801/151‑1 (MQ=255) tGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAAACCGCCAGGGGGGGCCGGGTAA‑ATAGTTaa > 1:307040/1‑147 (MQ=255) aTCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GG‑AAAAACTGCAAAGTGGGGGCCGGTAA‑GGAGGCAACCttt > 2:713403/1‑146 (MQ=255) acacAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCAttcttc < 1:441017/150‑1 (MQ=255) acacAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCg < 2:358994/151‑1 (MQ=255) cacaGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GG‑AAAAACTGCAAGGGGGGGCCGGGTAA‑AGAGTCAACCTGTGACCAttctt > 1:558688/1‑148 (MQ=255) caGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGGC‑GT‑GAAAACCGCGAGGGGGGAGCCGCAAA‑GGAGTCAAACTGGGAACATTTTTTGCTGATAACATCCttg > 1:221857/1‑149 (MQ=255) ggCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GT‑AAAAACCCCGAGGGGGGAGCGGGTAA‑AGAGTACAAATGGGACCAATCTTTCCTGGCATCAACCcggggg > 1:542947/1‑150 (MQ=255) tGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAA‑GT‑AAAAACCGCCAAGCGGGGCCGGAGAA‑GTAGTGGAACTGTGGACAATCTTCGCTTACATCATCCttgtgg > 1:94259/1‑147 (MQ=255) ggACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTg < 2:331873/149‑1 (MQ=255) aCGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACca < 2:846462/151‑1 (MQ=255) aCGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GT‑AAAAACCCCAAGGTGGGGGCGGGTAA‑GAAGGGAAACTGTGACCATTTTTCCCTGCCCTCATTCTGGGGGGTGGcc > 1:10524/1‑150 (MQ=255) ggTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATTGGGCGACTGGCGGGGAA‑GG‑AAAAAATGCCAGGGGGGGCCGGGAAA‑GAAGGTAACCTGTGACCAATCTTTGCCGACATCCTCCCGGTGGGTGACca > 1:67888/1‑149 (MQ=255) ttttttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GG‑AAAAACTGCAAGGGGGGGCCGGATAA‑ATAGTCAAACCGTGGCCCATTTTTGCTGACATCATCCTGGGGGGTGagc > 2:957334/1‑144 (MQ=255) tttttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GT‑AAAAACCGCAAAGGGGGGCCGGATAA‑ATAGGTAAACCGTGACCATTTTTCGCTGACATCATCCTGGGGGGTGAcc > 1:927007/1‑145 (MQ=255) ttttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GTGAAAACCGCCAAGG‑GGGGCCGGGGAA‑AAAGTCAAACCGTGACCATTCCTCTCTGACAGCATCCTTGTGGGTGGCcaca > 2:668570/1‑147 (MQ=255) tttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCAc < 1:404845/150‑1 (MQ=255) ttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACt < 1:1022057/150‑1 (MQ=255) tCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTg < 1:211918/150‑1 (MQ=255) cATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAAcgc < 2:693339/151‑1 (MQ=255) cATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GT‑AAAAACCGCAAGGGGGGGCCGGGTAA‑GTAGGCAAACCGTGACCATTTTTTGCTGACATCATCCTGGGGGGTGACCCCATCACTGaaa > 1:1054556/1‑148 (MQ=255) cATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GT‑AAAAACCGCAAAGGGGGGTCGGGTAA‑GTAGGTCACCCGTTGCCATTCTTCTCTGTCATTATCCTGGGGGCGGACCCAAACACTGa > 2:312325/1‑146 (MQ=255) atCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGt < 2:109766/151‑1 (MQ=255) ccACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAA‑GG‑AAAAACCGCAAAGGGGGGCCGGATAA‑ATAGTTCACCCGTGACCATTCTTCCCTGGCATCCTCCTGGTGGCGGGCCACCTCACTGAAAAGCGgggg > 1:1169151/1‑151 (MQ=255) cACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTggg < 1:821490/151‑1 (MQ=255) cGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGt < 1:836702/151‑1 (MQ=255) cGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGAAC‑GG‑TAAACACGCCAGGTGGGGGCGGGTAAGGAAGCCAACCT‑TGAACCCTCTTCCCTGAACACATCCTGGTGGGCGGCCACAACACCGAAAAGCGGAgggg > 1:634124/1‑150 (MQ=255) gtagtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAgg < 1:776870/151‑1 (MQ=255) agtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCtt < 1:156955/151‑1 (MQ=255) agtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCt < 1:606052/150‑1 (MQ=255) cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTTC‑GT‑AAAAACCGCAAGGGGGGACCGGATAA‑ATAGGGAACCCGTGACCCTTTTTCGCTGACATCATCCTGGTGGCTGACCACATCACCGGAAAGCGgggggggggggggccttaa > 1:366042/1‑136 (MQ=255) cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTAGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAAt < 2:593098/150‑1 (MQ=255) tGCCATCCTCGCAGCCATCGGGCGACTGGCGGGGAC‑GG‑AAAAACCGCAAGGGGGGGCCGGGTAA‑AGAGTCAACCCGTGACCATTCTTCGCTGACCTCATCCTTGTGGCTGACCCCAACACTGAAACGCGGGGGGGGGGGGGCCTTAAAttt > 2:483331/1‑151 (MQ=255) gCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAAtt < 1:78281/149‑1 (MQ=255) ccATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTAc < 2:937609/151‑1 (MQ=255) cTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGa < 2:806749/149‑1 (MQ=255) gcGCAGCCATCGGGCGACTGGCGGGGAC‑GT‑AAAAACTTCCAGGGGGGGCCGGATAA‑GAAGTTAACCCGTGACCCTTCTTCTCTTACATCATCCTTGTGGGTGACCACATCACTGAAAAGCGGGGGGGGGGGGGGCTTTAAttt > 2:217462/2‑143 (MQ=255) cGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGt < 1:1096403/150‑1 (MQ=255) gggCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtc < 2:51352/150‑1 (MQ=255) gggCGACTGGCGGGGAC‑GG‑AAAAACTGCAAGGGGGGGCCGGGTAA‑GGAGTCAACCTGTGGCCATTTTTCGCTGACATCATCCTGGTGGGTGACCACATCACTGAAACGCGGGGGGGGGGGGTCCTTAAATTTACTGAAGGCCTTCAgggc > 2:184364/1‑148 (MQ=255) ggCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtcat < 1:50228/151‑1 (MQ=255) tGGCGGGGAC‑GT‑AAAACCTGCAAGGGGGGAACGGATAA‑GTAGTCAACCTGTGACCATTTTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGGGGGGTGGGGTCCTTAAATTTACTGATGGCCGTCAGGTCATCACtt > 2:162696/1‑150 (MQ=255) ggCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTc < 1:396050/150‑1 (MQ=255) gT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGc > 2:977276/1‑150 (MQ=255) taaaCCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATg > 2:1169872/2‑151 (MQ=255) aaaaCCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAt < 1:939013/150‑1 (MQ=255) | CCGTCACCTCCTGGTATTCCAGTTTGATGTTATCGGCAATACACAGCGCATTCATCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTAC‑GT‑AAAACCTGCAAGGTGGGACCGGATAA‑GTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATG > NC_000913/266205‑266495 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |